Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,908 T→A 5.4% intergenic (+14/‑116) trkA → / → mscL NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,9080TA 5.4% 479.0 / 11.0 161intergenic (+14/‑116)trkA/mscLNAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Reads supporting (aligned to +/- strand):  ref base T (81/71);  new base A (5/4);  total (86/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 164 total aligned reads displayed.

ACGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGA  >  NC_000913/3437759‑3438057
                                                                                                                                                     |                                                                                                                                                     
aCGTGGAAACGACGTGATGATTGCCAATGACACTCTGCGCATTGAGCAAGGCGATCACGTAATGATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTACCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCt                                                                                                                                                       <  1:255527/150‑1 (MQ=255)
 cGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTa                                                                                                                                                      >  2:865266/1‑150 (MQ=255)
   tGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAAt                                                                                                                                                    >  1:1479576/1‑150 (MQ=255)
     gAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATgc                                                                                                                                                  >  2:505637/1‑149 (MQ=255)
      aaaCGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGAc                                                                                                                                                 <  1:889826/150‑1 (MQ=255)
          gacgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCt                                                                                                                                             <  2:410418/150‑1 (MQ=255)
           acgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCtt                                                                                                                                            <  1:1006154/150‑1 (MQ=255)
             gttattatTGCCAATGACAATCTGCTCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttat                                                                                                                                          <  1:292347/144‑1 (MQ=255)
             gTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGCCGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttat                                                                                                                                          <  1:1082796/150‑1 (MQ=255)
             gTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttat                                                                                                                                          >  2:873162/1‑150 (MQ=255)
              tgatgaTTGCCAATGACAATCTGCGGATTGAGCAAGGCGTTCACGTAATTATGTTCCTCTCAGATTAAATGTTTATTACCGACGTCGAAAGACTCTTCCAGGAAATCCATTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                         <  1:1347829/150‑1 (MQ=255)
               gatgatTGCCAATGACAATCTGCGCATTGAGCAAGGCGATTACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCttatta                                                                                                                                        <  2:1633705/150‑1 (MQ=255)
                     tGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTccc                                                                                                                                  <  1:941062/150‑1 (MQ=255)
                         aaTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTg                                                                                                                              <  2:132648/150‑1 (MQ=255)
                          aTGACAATCTGAGCATTGAGCAAGGCGATCACGTAATTATGTTCCTGACAGATAAAAAGGTTATTAGCGACGGCGAATGACTCTTCGAGCCAAGCCCTTTCTTCTCGTAATTAATAAGGCTTCTAATGACGCCTTATTATTTCCCTTTGa                                                                                                                             <  1:849008/150‑1 (MQ=255)
                               aaTCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATc                                                                                                                        <  1:916469/150‑1 (MQ=255)
                                  cTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAg                                                                                                                     <  1:954626/150‑1 (MQ=255)
                                  cTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAg                                                                                                                     <  1:783408/150‑1 (MQ=255)
                                           gAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatta                                                                                                            <  1:1012794/150‑1 (MQ=255)
                                             gCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTaaa                                                                                                          >  2:1239880/1‑150 (MQ=255)
                                             gCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTAACGACGTCGAAAGACTCTTCAGCCAAGCCCTTTCTTCTTGTAATTAATAATGAGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAat                                                                                                         <  1:514235/150‑1 (MQ=255)
                                              cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAat                                                                                                         <  1:373321/150‑1 (MQ=255)
                                               aaGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAATCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAatt                                                                                                        <  2:201046/150‑1 (MQ=255)
                                                aGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattc                                                                                                       <  1:1343027/150‑1 (MQ=255)
                                                    gATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattcatta                                                                                                   >  1:1586122/1‑149 (MQ=255)
                                                    gATAACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCATCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGTCGTCTAAAGACGCCTTATTATTTCCCTTTGATTATCAATGATTAATTAAattcatta                                                                                                   >  2:801094/1‑149 (MQ=255)
                                                       cACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAg                                                                                                                     <  1:1561584/129‑1 (MQ=255)
                                                       cACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAg                                                                                                                     >  2:1561584/1‑129 (MQ=255)
                                                       cACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTg                                                                                                >  2:263570/1‑150 (MQ=255)
                                                                  gTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATg                                                                                     <  1:1239880/150‑1 (MQ=255)
                                                                  gTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATg                                                                                     <  2:606029/150‑1 (MQ=255)
                                                                    tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGc                                                                                   <  1:1622483/150‑1 (MQ=255)
                                                                    tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGc                                                                                   <  1:194978/150‑1 (MQ=255)
                                                                     ccTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCt                                                                                  >  2:255992/1‑150 (MQ=255)
                                                                      cTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGTAAAATGGCtt                                                                                 >  1:844274/1‑150 (MQ=255)
                                                                      cTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCtt                                                                                 <  2:34914/150‑1 (MQ=255)
                                                                        cacaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTaa                                                                               >  2:31647/1‑150 (MQ=255)
                                                                        cacaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGAATTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTaa                                                                               >  2:812198/1‑150 (MQ=255)
                                                                        cacaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCAAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTaa                                                                               <  1:41298/150‑1 (MQ=255)
                                                                          caGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACa                                                                             >  1:749692/1‑150 (MQ=255)
                                                                          caGATAAAAAGTTTAATACCGACGTCGAAAGACTCTTCCATCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACa                                                                             <  2:219216/150‑1 (MQ=255)
                                                                            gATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAtt                                                                           <  2:647803/150‑1 (MQ=255)
                                                                            gATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAtt                                                                           >  2:1003489/1‑150 (MQ=255)
                                                                                 aaaGTTTATTTCCGACGTCGAAAGCCTCTTCCAGCCAAGCCCGTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTACCTTTGATTATCAAGGAGTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAGCATTTGttt                                                                      >  2:1231683/1‑149 (MQ=255)
                                                                                 aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                      <  2:372749/150‑1 (MQ=255)
                                                                                 aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                      >  1:354604/1‑150 (MQ=255)
                                                                                    gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTAATTCCTGGCAGGAAAATGGCTTAACATATGTTAGAc                                                                   >  1:1263886/1‑150 (MQ=255)
                                                                                      ttattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACtt                                                                 >  2:1726681/1‑150 (MQ=255)
                                                                                       tattaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTa                                                                >  1:935059/1‑150 (MQ=255)
                                                                                        attaCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTAt                                                               >  2:889689/1‑150 (MQ=255)
                                                                                           aacgtcGTCGAAATACTCTTCCTGACAAGACCTTTCTTCTTGGAATTAATAAGGCGTCTATTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTTAATTCATTCCTGGCAGGAAATTGGCTTAACATTTGTTAGACTTATGGt                                                            <  1:1634649/145‑1 (MQ=255)
                                                                                           aCCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGt                                                            <  1:758588/150‑1 (MQ=255)
                                                                                                cGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCg                                                       <  1:793053/150‑1 (MQ=255)
                                                                                                 gTCGAAAGCCTCTTCCAGCCAAGCCCGTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAAGTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACGTATTGTTGTCgg                                                      >  2:939378/1‑150 (MQ=255)
                                                                                                  tCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGTCTTAACATTTGTTAGACTTATGGTTGTCGGc                                                     >  1:812871/1‑150 (MQ=255)
                                                                                                   cGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCt                                                    <  1:879939/150‑1 (MQ=255)
                                                                                                   cGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCt                                                    >  2:1451506/1‑150 (MQ=255)
                                                                                                    gAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                   >  2:677507/1‑150 (MQ=255)
                                                                                                     aaaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGAGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTc                                                  <  2:428781/150‑1 (MQ=255)
                                                                                                       aGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCAt                                                <  2:1544740/150‑1 (MQ=255)
                                                                                                        gACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATa                                               <  2:1655494/150‑1 (MQ=255)
                                                                                                        gACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATa                                               >  2:1583386/1‑150 (MQ=255)
                                                                                                        gACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATa                                               <  1:558629/150‑1 (MQ=255)
                                                                                                          ctctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAgg                                             >  2:739247/1‑150 (MQ=255)
                                                                                                          ctctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAgg                                             <  1:1112997/150‑1 (MQ=255)
                                                                                                            ctTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGga                                           >  1:1519164/1‑150 (MQ=255)
                                                                                                             ttCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGgag                                          <  2:1173519/150‑1 (MQ=255)
                                                                                                                cAGCCAAGCCGTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAGATTCATTCCTGGCAGGAAAATGGATTAACATTTGTTAGACATATGGTTTTCGGCTTCATAGGGAGAAt                                       >  1:342503/1‑150 (MQ=255)
                                                                                                                cAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAAt                                       >  1:683673/1‑150 (MQ=255)
                                                                                                                 aGCAAATCCCTTTCTTATTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATa                                      <  1:339703/150‑1 (MQ=255)
                                                                                                                   ccAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATaaa                                    >  1:592296/1‑149 (MQ=255)
                                                                                                                    cAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACa                                   >  2:1283685/1‑150 (MQ=255)
                                                                                                                       gCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGa                                >  2:381628/1‑150 (MQ=255)
                                                                                                                         ccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGc                              >  2:10625/1‑150 (MQ=255)
                                                                                                                           tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCat                            <  2:1489606/150‑1 (MQ=255)
                                                                                                                              cttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGATTAACATGAGCattat                         >  2:1083794/1‑150 (MQ=255)
                                                                                                                              cttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattat                         >  1:549339/1‑150 (MQ=255)
                                                                                                                              cttcttGTAATTAATAAGGCGTCTAATGAAGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCATGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGTAGAATAACATGAGCattat                         >  1:1369605/1‑150 (MQ=255)
                                                                                                                               ttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCATGAATATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattatt                        <  1:1092544/150‑1 (MQ=255)
                                                                                                                               ttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattatt                        <  1:263570/150‑1 (MQ=255)
                                                                                                                                tcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATa                                      <  2:1643353/135‑1 (MQ=255)
                                                                                                                                tcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCAttatta                       >  1:1236842/1‑150 (MQ=255)
                                                                                                                                  ttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaaa                     <  1:889689/150‑1 (MQ=255)
                                                                                                                                   tGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAg                    <  1:987118/150‑1 (MQ=255)
                                                                                                                                    gTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGa                   >  1:322803/1‑150 (MQ=255)
                                                                                                                                       attaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAtt                >  2:633441/1‑150 (MQ=255)
                                                                                                                                       aataataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAtt                >  2:1168447/3‑150 (MQ=255)
                                                                                                                                        ataataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAAttt               >  2:963939/2‑150 (MQ=255)
                                                                                                                                         taataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTc              >  2:561566/1‑150 (MQ=255)
                                                                                                                                          aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg             <  2:837580/150‑1 (MQ=255)
                                                                                                                                           ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgg            >  2:670849/1‑149 (MQ=255)
                                                                                                                                           ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc            >  1:485716/1‑150 (MQ=255)
                                                                                                                                           ataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgc            >  1:381709/1‑150 (MQ=255)
                                                                                                                                           ataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcga            >  2:1513317/1‑149 (MQ=255)
                                                                                                                                            taaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgcg           >  2:937136/1‑150 (MQ=255)
                                                                                                                                                 cGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAttt      >  2:1179503/1‑150 (MQ=255)
                                                                                                                                                  gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg     >  2:1205538/1‑150 (MQ=255)
                                                                                                                                                  gTCTAATGACGCCTTATTATTTACCTTTTATTATCAAGTATTTATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAATTGTTAGACTTATGGTTGTCGGCCTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg     <  1:1564240/150‑1 (MQ=255)
                                                                                                                                                    cTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCg   <  2:195144/150‑1 (MQ=255)
                                                                                                                                                     tAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATCGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGa  <  1:316602/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGA  >  NC_000913/3437759‑3438057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: