Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,439,588 G→C 9.5% intergenic (‑79/+28) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,5880GC 9.5% 409.3 / 27.2 135intergenic (‑79/+28)yhdN/rplQDUF1992 family protein/50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base G (57/65);  new base C (9/4);  total (66/69)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

Only 100 of 143 total aligned reads displayed.

AGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCG  >  NC_000913/3439439‑3439737
                                                                                                                                                        |                                                                                                                                                     
aGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACccgccg                                                                                                                                                       >  2:1044750/1‑150 (MQ=255)
aGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACccgccg                                                                                                                                                       >  2:390844/1‑150 (MQ=255)
 ggTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCgg                                                                                                                                                      >  2:940448/1‑150 (MQ=255)
  gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCggg                                                                                                                                                     >  2:423749/1‑150 (MQ=255)
  gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAACCCGCCgggg                                                                                                                                                    <  2:1704666/150‑1 (MQ=255)
   ttATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCgggg                                                                                                                                                    >  2:1368268/1‑150 (MQ=255)
    tATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGc                                                                                                                                                   <  1:717548/150‑1 (MQ=255)
    tATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGc                                                                                                                                                   <  1:1276312/150‑1 (MQ=255)
    tATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGc                                                                                                                                                   <  1:1578759/150‑1 (MQ=255)
    tATCAAACTCACCTTTCGCTAGCGCTGCTGCTATATGCCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGc                                                                                                                                                   <  2:1055196/150‑1 (MQ=255)
     aTCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCg                                                                                                                                                  >  2:362293/1‑150 (MQ=255)
       cAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCggg                                                                                                                                                <  2:1220481/150‑1 (MQ=255)
       cAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCggg                                                                                                                                                <  2:896025/150‑1 (MQ=255)
       cAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCAGGGGCggg                                                                                                                                                >  1:1502678/1‑150 (MQ=255)
       aaaaTTCAGCTTGCGCTTGCGCTTCTGCTATATCGCGCTCGGCCCAGTGGTAAATAAACAACATAAATCCTCCCGGGCTTGACCAAAACGTGTACAGTTATTTTAGTAAAGTGGGTATATTAGGGTTAT‑‑AAAAAAACCCGCCGGGGCggg                                                                                                                                                <  2:469433/149‑1 (MQ=255)
         aaCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGtt                                                                                                                                              >  1:1015477/1‑150 (MQ=255)
          aCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGttt                                                                                                                                             <  1:512545/150‑1 (MQ=255)
             cACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                          <  1:1359823/150‑1 (MQ=255)
             cACCTTTCGCTAGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAA‑‑AAAAAAACCCGCCGGGGCGGGtttttt                                                                                                                                          <  1:48707/150‑1 (MQ=255)
              aCCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCCCGGCGGGttttttt                                                                                                                                         <  1:816101/150‑1 (MQ=255)
                cattCGCGTGCGCTTCTGCTATCTGGCGCTCTGCCCACTGGGCAAGTAACCACATAAATACTCCCGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAA‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTAc                                                                                                                                       <  1:1408071/148‑1 (MQ=255)
                cTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAACTACTCCAGGGGCTGAACAAAACGATTACAGCTATTGTAGATAAGTGTGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCTGGTTTTTTTAc                                                                                                                                       <  1:1401063/150‑1 (MQ=255)
                         gcgcTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCa                                                                                                                              <  1:1490042/150‑1 (MQ=255)
                               cTGCTATATGGCGCTCAGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATCAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAc                                                                                                                        <  1:604066/150‑1 (MQ=255)
                               cTGATATATGGCGCTCTGCCCACTGGTCAAGGAACCACATAAATACTCCAGGGGATGAACAAAAAGAGGACAGCTATTGTAGATAAGTGGGGATCCGACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGGTGTTGGTCGTTGCTTCAGATGAc                                                                                                                        >  1:131105/1‑150 (MQ=255)
                                tGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGTGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAct                                                                                                                       <  2:195768/150‑1 (MQ=255)
                                tGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACAAAAATAATCCAGGGGATTAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAct                                                                                                                       <  2:210282/150‑1 (MQ=255)
                                 gCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTActc                                                                                                                      >  1:541966/1‑150 (MQ=255)
                                 gCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTActc                                                                                                                      >  1:1177504/1‑150 (MQ=255)
                                 gCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTAAGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGATTTTTTACGTTGCTTCAGATGActc                                                                                                                      >  1:1331738/1‑150 (MQ=255)
                                     tatGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgca                                                                                                                  >  1:1242394/1‑150 (MQ=255)
                                       tGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcagc                                                                                                                >  2:769926/1‑150 (MQ=255)
                                        ggCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGTTGAACAAAAAGAGTACAGCTATTGTAGATAAGGGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcagca                                                                                                               <  1:875364/150‑1 (MQ=255)
                                           gcTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTGCAGAGTAATCTgaagcaacg                                                                                                            <  1:1044750/150‑4 (MQ=255)
                                                 cccACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGGTTTTTTACGTTGCGTCAGATTACTCTGCAGCAGCTTCTGCt                                                                                                      >  2:1120553/1‑150 (MQ=255)
                                                      tGGTCAAGTAACCACATAAATACTCCCGGGGATGAACAAAAAGAGTCCAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtctc                                                                                                 <  2:892716/150‑1 (MQ=255)
                                                      tGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtctc                                                                                                 <  1:736797/150‑1 (MQ=255)
                                                       ggTCAAGTAACCACATAAATATTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                                                                                >  1:1102210/1‑150 (MQ=255)
                                                       ggTCAAGTAACAACATAAATACTCCAGGGGATTAACAAAAATAGTACAGCTATTGTAGATAATTGTGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                                                                                <  2:270508/150‑1 (MQ=255)
                                                        gTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTg                                                                                               <  2:1527527/150‑1 (MQ=255)
                                                          cAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGaa                                                                                             <  1:643742/150‑1 (MQ=255)
                                                            aGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACg                                                                                           >  1:1144758/1‑150 (MQ=255)
                                                                aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCa                                                                                       <  2:1271916/150‑1 (MQ=255)
                                                                   acaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAcc                                                                                    <  2:1618675/150‑1 (MQ=255)
                                                                   acaTAAATACTCAAGGGGATTAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCTGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAcc                                                                                    <  2:1316810/150‑1 (MQ=255)
                                                                    caTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCa                                                                                   >  2:451941/1‑150 (MQ=255)
                                                                      tAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCCGc                                                                                 >  2:159130/1‑150 (MQ=255)
                                                                             tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGt                                                                          <  1:1249933/150‑1 (MQ=255)
                                                                              ccAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTa                                                                         >  2:731420/1‑150 (MQ=255)
                                                                                aGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTAGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAg                                                                       <  2:1144384/150‑1 (MQ=255)
                                                                                 ggggATGAACATAAAGAGTACAGTTTTTGTAGTTTAGTTGGGTTACTACGGGTTT‑‑AAAATAACCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAATCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAg                                                                       <  1:1530935/150‑1 (MQ=255)
                                                                                       gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg                                                                >  2:1383140/1‑150 (MQ=255)
                                                                                       gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg                                                                >  2:1348536/1‑150 (MQ=255)
                                                                                         aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg                                                                >  1:1555063/1‑150 (MQ=255)
                                                                                         aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                              >  2:461681/1‑150 (MQ=255)
                                                                                          cAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcgc                                                             >  1:1176575/1‑150 (MQ=255)
                                                                                           aaaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGcgcg                                                            >  1:724320/1‑150 (MQ=255)
                                                                                             aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGtt                                                          >  2:952705/1‑150 (MQ=255)
                                                                                              aaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAATCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTg                                                         <  1:626052/150‑1 (MQ=255)
                                                                                                    aCAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCt                                                   >  2:565704/1‑150 (MQ=255)
                                                                                                     cAGCTTTTGCAGATAAGTGGGGTTACTACGGGTAT‑‑AAAATAACCGGGCGGGGCGGGTTTTTTTACGTTGGTTCAGATTTCTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCATCGTGCTCGATGTAAGCCATCGGCGCGTTGTCGCGTg                                                  <  2:1603756/150‑1 (MQ=255)
                                                                                                     cAGCTATTGTAGTTAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTg                                                  <  1:220535/150‑1 (MQ=255)
                                                                                                      aGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGc                                                 >  1:1153104/1‑150 (MQ=255)
                                                                                                      aGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGc                                                 <  2:1233819/150‑1 (MQ=255)
                                                                                                      aGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGc                                                 <  1:362293/150‑1 (MQ=255)
                                                                                                       gCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCa                                                >  2:1390779/1‑150 (MQ=255)
                                                                                                         tATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACg                                              >  1:1038416/1‑150 (MQ=255)
                                                                                                            tGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAGCAGATCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGaa                                           <  2:848555/150‑1 (MQ=255)
                                                                                                             gTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTGTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAg                                          >  1:337004/1‑150 (MQ=255)
                                                                                                               aGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTACTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGcc                                        >  1:1673530/1‑150 (MQ=255)
                                                                                                                gATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGccc                                       >  2:607939/1‑149 (MQ=255)
                                                                                                                 aTAAGTGGGGATACTACGGGTAT‑‑AAAAACACCCGCCGGGGCGGGTTTTTTTACGTTGCGTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCcac                                      <  1:295060/150‑1 (MQ=255)
                                                                                                                 aTAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGcccc                                      >  2:860788/1‑148 (MQ=255)
                                                                                                                    aGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACt                                   >  2:404418/1‑150 (MQ=255)
                                                                                                                     gTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACtt                                  >  2:1382628/1‑150 (MQ=255)
                                                                                                                      gggggATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTc                                 <  2:1456444/149‑1 (MQ=255)
                                                                                                                        gggATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAg                               <  2:296480/150‑1 (MQ=255)
                                                                                                                         ggATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACCCTTCAGa                              >  2:1329916/1‑150 (MQ=255)
                                                                                                                          gATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGaa                             <  1:1383140/150‑1 (MQ=255)
                                                                                                                          gATACTACGGGTAA‑‑AAAAATACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGaa                             <  2:725795/150‑1 (MQ=255)
                                                                                                                            tactacGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATa                           <  2:1102210/150‑1 (MQ=255)
                                                                                                                                ccGGCTAT‑‑ACAAAAACCCGCCGGGGCGGGTTCTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCTCCTGCACGGAAGCCACACTTCAGAATACGAg                       <  2:1134604/149‑1 (MQ=255)
                                                                                                                                acGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTTCTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCCTCGGCGCGTTGTCGCCTGCACGGAAGCCGCACTTCAGAATACGAg                       >  1:94311/1‑150 (MQ=255)
                                                                                                                                  gggTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAgtg                     <  1:584597/150‑1 (MQ=255)
                                                                                                                                   aacgt‑‑aAAAAAACCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCGTCTGCTTTCTCTGAACGCTCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGGCTGCACGGAAGCCACACTTCTGAATACGAgtgt                    >  1:1467142/5‑150 (MQ=255)
                                                                                                                                    acgt‑‑aAAAAAACCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTTCACGGAAGCCACACTTCAGAATACGAGTGTa                   >  1:39934/4‑150 (MQ=255)
                                                                                                                                    acgt‑‑aAAAAAACCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTa                   >  1:1714578/4‑150 (MQ=255)
                                                                                                                                       t‑‑aAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTCGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacc                >  2:1352117/1‑150 (MQ=255)
                                                                                                                                          aaaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGACCGATCATCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGACTGCAAGGAAGCCACCCTGCAGAATAAGAGTGTCaccc               >  1:1667051/1‑149 (MQ=255)
                                                                                                                                          aaaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacca               >  2:1169534/1‑150 (MQ=255)
                                                                                                                                          aaaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacca               >  2:527130/1‑150 (MQ=255)
                                                                                                                                           aaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCCTCGGCGCGTTGTCGCCTGCACGGAAGCCCCACTGCAGAATACGAGTGTAacccc              >  2:1578403/1‑148 (MQ=255)
                                                                                                                                           aaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacctc              >  1:11222/1‑148 (MQ=255)
                                                                                                                                           aaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac              >  2:789497/1‑150 (MQ=255)
                                                                                                                                           aaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac              >  2:812270/1‑150 (MQ=255)
                                                                                                                                            aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAGCGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGGAcccacc             >  1:265487/1‑150 (MQ=255)
                                                                                                                                               aaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCgg           <  1:99479/150‑1 (MQ=255)
                                                                                                                                                  cccGCCGTGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCTATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCAc        <  1:291463/150‑1 (MQ=255)
                                                                                                                                                  cccGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCAc        >  1:434348/1‑150 (MQ=255)
                                                                                                                                                        ggggCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCACCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGACGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTcg  >  2:1698595/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
AGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCG  >  NC_000913/3439439‑3439737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: