Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,439,589 G→C 9.5% intergenic (‑80/+27) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,5890GC 9.5% 388.8 / 27.3 136intergenic (‑80/+27)yhdN/rplQDUF1992 family protein/50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base G (58/64);  new base C (9/4);  total (67/69)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-02

Only 100 of 142 total aligned reads displayed.

GGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGC  >  NC_000913/3439440‑3439738
                                                                                                                                                       |                                                                                                                                                      
ggTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCgg                                                                                                                                                        >  2:940448/1‑150 (MQ=255)
 gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCggg                                                                                                                                                       >  2:423749/1‑150 (MQ=255)
 gTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAACCCGCCgggg                                                                                                                                                      <  2:1704666/150‑1 (MQ=255)
  ttATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCgggg                                                                                                                                                      >  2:1368268/1‑150 (MQ=255)
   tATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGc                                                                                                                                                     <  1:1578759/150‑1 (MQ=255)
   tATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGc                                                                                                                                                     <  1:1276312/150‑1 (MQ=255)
    aTCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCg                                                                                                                                                    >  2:362293/1‑150 (MQ=255)
      cAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCggg                                                                                                                                                  <  2:1220481/150‑1 (MQ=255)
      cAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCggg                                                                                                                                                  <  2:896025/150‑1 (MQ=255)
      cAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCAGGGGCggg                                                                                                                                                  >  1:1502678/1‑150 (MQ=255)
      aaaaTTCAGCTTGCGCTTGCGCTTCTGCTATATCGCGCTCGGCCCAGTGGTAAATAAACAACATAAATCCTCCCGGGCTTGACCAAAACGTGTACAGTTATTTTAGTAAAGTGGGTATATTAGGGTTAT‑‑AAAAAAACCCGCCGGGGCggg                                                                                                                                                  <  2:469433/149‑1 (MQ=255)
        aaCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGtt                                                                                                                                                >  1:1015477/1‑150 (MQ=255)
        aaCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGtt                                                                                                                                                <  2:1388252/150‑1 (MQ=255)
        aaCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTACAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACACGCCGGGGCGGGtt                                                                                                                                                >  1:876194/1‑150 (MQ=255)
         aCTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGttt                                                                                                                                               <  1:512545/150‑1 (MQ=255)
          cTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCCCGGCGGGtttt                                                                                                                                              <  2:408802/150‑1 (MQ=255)
           tCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAACTACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGGGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCCCGGCGGGttttt                                                                                                                                             <  1:1511572/150‑1 (MQ=255)
               cattCGCGTGCGCTTCTGCTATCTGGCGCTCTGCCCACTGGGCAAGTAACCACATAAATACTCCCGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAA‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTAc                                                                                                                                        <  1:1408071/148‑1 (MQ=255)
               cTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAACTACTCCAGGGGCTGAACAAAACGATTACAGCTATTGTAGATAAGTGTGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCTGGTTTTTTTAc                                                                                                                                        <  1:1401063/150‑1 (MQ=255)
                tttCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGGCAATTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AATAAAACCCGCCGGGGCGGGTTTTTTTACg                                                                                                                                       <  1:1161958/150‑1 (MQ=255)
                     cTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACTAAAAGAGTACAGCTATTGTAGATAAGTGGGGATAGTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTGTTTTAACGTTGCt                                                                                                                                  >  2:102100/1‑150 (MQ=255)
                              cTGCTATATGGCGCTCAGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATCAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAc                                                                                                                         <  1:604066/150‑1 (MQ=255)
                              cTGATATATGGCGCTCTGCCCACTGGTCAAGGAACCACATAAATACTCCAGGGGATGAACAAAAAGAGGACAGCTATTGTAGATAAGTGGGGATCCGACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGGTGTTGGTCGTTGCTTCAGATGAc                                                                                                                         >  1:131105/1‑150 (MQ=255)
                               tGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGTGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAct                                                                                                                        <  2:195768/150‑1 (MQ=255)
                                gCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTActc                                                                                                                       >  1:1177504/1‑150 (MQ=255)
                                gCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTActc                                                                                                                       <  1:700119/150‑1 (MQ=255)
                                gCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTActc                                                                                                                       >  1:541966/1‑150 (MQ=255)
                                gCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTAAGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGATTTTTTACGTTGCTTCAGATGActc                                                                                                                       >  1:1331738/1‑150 (MQ=255)
                                   atatGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATCCTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgt                                                                                                                    <  2:1174919/150‑2 (MQ=255)
                                    tatGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgca                                                                                                                   >  1:1242394/1‑150 (MQ=255)
                                      tGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcagc                                                                                                                 >  2:769926/1‑150 (MQ=255)
                                       ggCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGTTGAACAAAAAGAGTACAGCTATTGTAGATAAGGGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcagca                                                                                                                <  1:875364/150‑1 (MQ=255)
                                          gcTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTGCAGAGTAATCTgaagcaacg                                                                                                             <  1:1044750/150‑4 (MQ=255)
                                               gCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGc                                                                                                        <  1:443436/150‑1 (MQ=255)
                                                  cACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGAGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCttt                                                                                                     >  2:1355940/1‑150 (MQ=255)
                                                     tGGTCAAGTAACCACATAAATACTCCCGGGGATGAACAAAAAGAGTCCAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtctc                                                                                                  <  2:892716/150‑1 (MQ=255)
                                                     tGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtctc                                                                                                  <  1:736797/150‑1 (MQ=255)
                                                      ggTCAAGTAACCACATAAATATTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                                                                                 >  1:1102210/1‑150 (MQ=255)
                                                      ggTCAAGTAACAACATAAATACTCCAGGGGATTAACAAAAATAGTACAGCTATTGTAGATAATTGTGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                                                                                 <  2:270508/150‑1 (MQ=255)
                                                       gTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTg                                                                                                <  2:1527527/150‑1 (MQ=255)
                                                         cAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGaa                                                                                              <  1:643742/150‑1 (MQ=255)
                                                          aaGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAACCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAAc                                                                                             <  1:739390/150‑1 (MQ=255)
                                                           aGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACg                                                                                            >  1:1144758/1‑150 (MQ=255)
                                                           aGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACg                                                                                            <  1:621651/150‑1 (MQ=255)
                                                               aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCa                                                                                        <  2:1271916/150‑1 (MQ=255)
                                                                  acaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAcc                                                                                     <  2:1618675/150‑1 (MQ=255)
                                                                   caTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCa                                                                                    >  2:451941/1‑150 (MQ=255)
                                                                     tAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCCGc                                                                                  >  2:159130/1‑150 (MQ=255)
                                                                            tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGt                                                                           <  1:1249933/150‑1 (MQ=255)
                                                                             ccAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTa                                                                          >  2:731420/1‑150 (MQ=255)
                                                                                      gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg                                                                 >  2:1383140/1‑150 (MQ=255)
                                                                                        aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg                                                                 >  1:1555063/1‑150 (MQ=255)
                                                                                        aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCTTCGgcg                                                               <  1:227571/150‑1 (MQ=255)
                                                                                        aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                               >  2:461681/1‑150 (MQ=255)
                                                                                         cAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTATACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcg                                                               >  2:834407/1‑150 (MQ=255)
                                                                                          aaaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGcgcg                                                             >  1:724320/1‑150 (MQ=255)
                                                                                          aaaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGcgcg                                                             >  2:1590317/1‑150 (MQ=255)
                                                                                            aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGtt                                                           >  2:1134921/1‑150 (MQ=255)
                                                                                            aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGtt                                                           >  2:952705/1‑150 (MQ=255)
                                                                                             aatagTACAGCTCTTGTAGTTAAGTGGGGATACTACGGGTAT‑‑AAAAAACCCCGCCGGCGCGGGTTTTTTTACGTTGATTCTGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGTCGCGTTg                                                          <  2:1331738/147‑1 (MQ=255)
                                                                                              agagTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGt                                                         >  1:1487481/1‑150 (MQ=255)
                                                                                                   aCAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCt                                                    >  2:565704/1‑150 (MQ=255)
                                                                                                    cAGCTATTGTAGTTAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTg                                                   <  1:220535/150‑1 (MQ=255)
                                                                                                     aGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGc                                                  >  1:1153104/1‑150 (MQ=255)
                                                                                                     aGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGc                                                  <  1:362293/150‑1 (MQ=255)
                                                                                                       cTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCAc                                                >  2:1289246/1‑150 (MQ=255)
                                                                                                        tATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACg                                               >  1:1038416/1‑150 (MQ=255)
                                                                                                        tATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACg                                               <  1:565704/150‑1 (MQ=255)
                                                                                                           tGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAGCAGATCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGaa                                            <  2:848555/150‑1 (MQ=255)
                                                                                                            gTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAg                                           >  1:393733/1‑150 (MQ=255)
                                                                                                            gTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTGTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAg                                           >  1:337004/1‑150 (MQ=255)
                                                                                                              aGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGcc                                         >  2:184638/1‑150 (MQ=255)
                                                                                                              aGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTACTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGcc                                         >  1:1673530/1‑150 (MQ=255)
                                                                                                               gATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGccc                                        >  2:607939/1‑149 (MQ=255)
                                                                                                                aTAAGTGGGGATACTACGGGTAT‑‑AAAAACACCCGCCGGGGCGGGTTTTTTTACGTTGCGTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCcac                                       <  1:295060/150‑1 (MQ=255)
                                                                                                                aTAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGcccc                                       >  2:860788/1‑148 (MQ=255)
                                                                                                                     tGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTc                                  <  1:1348536/150‑1 (MQ=255)
                                                                                                                     gggggATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTc                                  <  2:1456444/149‑1 (MQ=255)
                                                                                                                        ggATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACCCTTCAGa                               >  2:1329916/1‑150 (MQ=255)
                                                                                                                         gATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGaa                              <  1:1383140/150‑1 (MQ=255)
                                                                                                                         gATACTACGGGTAA‑‑AAAAATACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGaa                              <  2:725795/150‑1 (MQ=255)
                                                                                                                           tactacGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATa                            <  2:1102210/150‑1 (MQ=255)
                                                                                                                            actacGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTATCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATAc                           >  2:385396/1‑150 (MQ=255)
                                                                                                                               ccGGCTAT‑‑ACAAAAACCCGCCGGGGCGGGTTCTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCTCCTGCACGGAAGCCACACTTCAGAATACGAg                        <  2:1134604/149‑1 (MQ=255)
                                                                                                                               acGGGTAT‑‑AAAAAAACCCGCCGGGGAGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAg                        <  2:1116248/150‑1 (MQ=255)
                                                                                                                                 gggTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAgtg                      <  2:1513507/150‑1 (MQ=255)
                                                                                                                                  aacgt‑‑aAAAAAACCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCGTCTGCTTTCTCTGAACGCTCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGGCTGCACGGAAGCCACACTTCTGAATACGAgtgt                     >  1:1467142/5‑150 (MQ=255)
                                                                                                                                   gTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAg                                <  1:1696766/138‑1 (MQ=255)
                                                                                                                                   gTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAg                                >  2:1696766/1‑138 (MQ=255)
                                                                                                                                      t‑‑aAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacc                 >  2:23500/1‑150 (MQ=255)
                                                                                                                                      t‑‑aAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTCGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacc                 >  2:1352117/1‑150 (MQ=255)
                                                                                                                                         aaaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGACCGATCATCCAGCTCGATGTAAGCCATCGGCGCGTTGTCGACTGCAAGGAAGCCACCCTGCAGAATAAGAGTGTCaccc                >  1:1667051/1‑149 (MQ=255)
                                                                                                                                         aaaaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacca                >  2:1169534/1‑150 (MQ=255)
                                                                                                                                          aaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGTAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac               >  2:560667/1‑150 (MQ=255)
                                                                                                                                          aaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAacctc               >  1:11222/1‑148 (MQ=255)
                                                                                                                                          aaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAaccac               >  2:789497/1‑150 (MQ=255)
                                                                                                                                             aaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCgg            <  1:99479/150‑1 (MQ=255)
                                                                                                                                                cccGCCGTGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCTATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCAc         <  1:291463/150‑1 (MQ=255)
                                                                                                                                                cccGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCAc         >  1:434348/1‑150 (MQ=255)
                                                                                                                                                       gggCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTcgc  <  2:1109407/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
GGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTAT‑‑AAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGC  >  NC_000913/3439440‑3439738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: