Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,441,061 G→C 10.0% S205S (TCC→TCG) ‡ rpsD ← 30S ribosomal subunit protein S4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,441,0610GC10.0% 392.0 / 26.3 129S205S (TCC→TCG) ‡rpsD30S ribosomal subunit protein S4
Reads supporting (aligned to +/- strand):  ref base G (64/52);  new base C (8/5);  total (72/57)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

Only 100 of 130 total aligned reads displayed.

CAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTT  >  NC_000913/3440913‑3441209
                                                                                                                                                    |                                                                                                                                                    
        ggCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGc                                                                                                                                             <  1:48993/150‑1 (MQ=255)
         gCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCt                                                                                                                                            >  2:1415479/1‑150 (MQ=255)
          ccAAAGCCACGCTCTACAGGCTCAAGGGGCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTc                                                                                                                                           <  2:496250/150‑1 (MQ=255)
          ccAAAGCCACGCTCTAAAGGCTCAAGGGTCCCCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTc                                                                                                                                           <  2:1377021/150‑1 (MQ=255)
          ccAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTc                                                                                                                                           >  1:1026568/1‑150 (MQ=255)
           cAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTCCAAGTAAAGCtta                                                                                                                                          <  1:558849/150‑3 (MQ=255)
            aaaGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTCCAAGTAAAGCttag                                                                                                                                         <  1:529861/150‑4 (MQ=255)
             aaGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcgac                                                                                                                                        <  1:1613279/150‑1 (MQ=255)
              aGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcgacg                                                                                                                                       >  2:791825/1‑150 (MQ=255)
                 cACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCCAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCAAAGAACCCTGAATTGTGTCCTCTCTATGGTACTAAGCTATACTTGGAGTAAAGCTCGACGATc                                                                                                                                    >  2:1308204/1‑150 (MQ=255)
                    gCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAgg                                                                                                                                 <  1:1272994/150‑1 (MQ=255)
                    gCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGGCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAgg                                                                                                                                 <  2:244660/150‑1 (MQ=255)
                      gctAAAGGCGCCAGGGGCACCTTGGCGTGCGGCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGGCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgtg                                                                                                                               <  2:1344736/149‑1 (MQ=255)
                       ctAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGCACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgtgt                                                                                                                              >  2:1099782/1‑150 (MQ=255)
                       ctAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGgtgt                                                                                                                              <  2:778023/150‑1 (MQ=255)
                        tAAAGTCTCAAGTGGCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGtt                                                                                                                             <  1:708566/150‑1 (MQ=255)
                         aaaGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTc                                                                                                                            >  2:897530/1‑150 (MQ=255)
                         aaaGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTc                                                                                                                            <  2:210223/150‑1 (MQ=255)
                            ggCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGtt                                                                                                                         >  2:149946/1‑150 (MQ=255)
                            ggCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCGCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGGGTTCGtt                                                                                                                         >  2:311857/1‑150 (MQ=255)
                               tCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGAACACCTGATCGtcgag                                                                                                                      <  1:624860/150‑5 (MQ=255)
                                     gTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGc                                                                                                                <  1:375472/150‑1 (MQ=255)
                                         ccTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCATGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCATTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcagac                                                                                                            >  1:1188867/1‑150 (MQ=255)
                                         ccTTGGCGTGCGTCGAACTCACTAGCTCGATATCAACAAGGCGCGGTTTTAGAAACTCTGACACAGAACCCTGCAATGTGTCCTCTCATTGGTACTAAGCTTTAGTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcagac                                                                                                            >  2:940241/1‑150 (MQ=255)
                                           ttGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcagacag                                                                                                          >  1:1567635/1‑150 (MQ=255)
                                           ttGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcagacag                                                                                                          >  1:482407/1‑150 (MQ=255)
                                            tGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcagacaga                                                                                                         >  1:538241/1‑150 (MQ=255)
                                            tGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGcagacaga                                                                                                         >  1:132649/1‑150 (MQ=255)
                                                    gtCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACg                                                                                                 >  2:370301/1‑150 (MQ=255)
                                                        aaCTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTcc                                                                                             >  1:633986/1‑150 (MQ=255)
                                                         aCTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCg                                                                                            <  1:791825/150‑1 (MQ=255)
                                                         aCTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCg                                                                                            <  2:1469303/150‑1 (MQ=255)
                                                          cTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGACCGCTCCgg                                                                                           >  2:1614624/1‑150 (MQ=255)
                                                          cTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCgg                                                                                           >  2:1694036/1‑150 (MQ=255)
                                                           tCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGc                                                                                          <  2:494126/150‑1 (MQ=255)
                                                            cACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCt                                                                                         >  2:985423/1‑150 (MQ=255)
                                                                tGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACg                                                                                     >  2:893321/1‑150 (MQ=255)
                                                                    cGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTa                                                                                 <  1:464463/150‑1 (MQ=255)
                                                                    cGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTa                                                                                 >  1:917130/1‑150 (MQ=255)
                                                                       tatCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGAGGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAc                                                                              >  2:719985/1‑150 (MQ=255)
                                                                        atCAAGCAGGCGCGGTTTTAGAAACTCTGTCACAGAACGCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACg                                                                             <  1:296011/150‑1 (MQ=255)
                                                                         tCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGt                                                                            >  1:1406227/1‑150 (MQ=255)
                                                                         tCAACCAGGCGCGGGGTTAGAATCTCTGTCACAGCACACTGCATTGTGTCCTCTATTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCGTAAACGt                                                                            <  1:73937/150‑1 (MQ=255)
                                                                          cAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTa                                                                           >  2:1482748/1‑150 (MQ=255)
                                                                          cAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTa                                                                           >  1:203042/1‑150 (MQ=255)
                                                                               aGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTc                                                                      >  2:1023793/1‑150 (MQ=255)
                                                                                    gggTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCATTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCt                                                                 <  1:1584670/149‑1 (MQ=255)
                                                                                     ggTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCtt                                                                <  2:1033268/150‑1 (MQ=255)
                                                                                      gTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGATTAAAGATCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTg                                                               <  1:1308204/150‑1 (MQ=255)
                                                                                        tttAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGcc                                                             >  2:497797/1‑150 (MQ=255)
                                                                                          tAGAAACTCTGTCACAGAACCCTGCA‑TGTGTCCACGGGTTGGTACTAAGCATTACCTGGAGTTAAGCGCGCCGCTCCGGTGTTCCTTAATGTCCGCAGCCAGATCACAACGCTCCGGCTTACGCTTAAAAGTACCTTCCAACTAGCCAGc                                                          >  2:111736/1‑150 (MQ=255)
                                                                                           aGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGc                                                          >  2:826691/1‑150 (MQ=255)
                                                                                           aGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGc                                                          >  2:852915/1‑150 (MQ=255)
                                                                                            gAAACTCTGTCACATAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCa                                                         <  1:1447878/150‑1 (MQ=255)
                                                                                            gAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCa                                                         <  1:1284026/150‑1 (MQ=255)
                                                                                            gAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCa                                                         >  2:1574953/1‑150 (MQ=255)
                                                                                              aaCTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATc                                                       <  1:223331/150‑1 (MQ=255)
                                                                                                 tctGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAAc                                                    <  1:1023793/150‑1 (MQ=255)
                                                                                                 tctGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAAc                                                    <  2:702346/150‑1 (MQ=255)
                                                                                                  ctGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACt                                                   <  1:1087512/150‑1 (MQ=255)
                                                                                                   tGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACtt                                                  >  2:980099/1‑150 (MQ=255)
                                                                                                    gTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGAAAAGATCTACGATCAGGTGTTCTTTAATGTCCGCAGACAGATCAGAACCCACCGGCTTACGATAAAACTTACCTACCATCTTGCCAGAATCAACtac                                                 >  1:879469/1‑148 (MQ=255)
                                                                                                     tCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTAGACTATCATTTGTTCGTTAATTTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTcc                                                <  1:506842/150‑1 (MQ=255)
                                                                                                      cacaGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTcca                                               <  2:1334665/150‑1 (MQ=255)
                                                                                                      cacaGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGACAGCATCAACTTcca                                               >  2:1179583/1‑150 (MQ=255)
                                                                                                               cTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCACCTTCCAGCCAGGTTg                                      >  2:45362/1‑150 (MQ=255)
                                                                                                               cTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTg                                      <  1:1694036/150‑1 (MQ=255)
                                                                                                                tGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTgg                                     >  2:562912/1‑150 (MQ=255)
                                                                                                                      gtgtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCtttta                               >  2:307483/1‑149 (MQ=255)
                                                                                                                        gtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATGAACTTCCAGCCAGGTTGGCTTTTCAc                             <  2:937336/150‑1 (MQ=255)
                                                                                                                        gtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCAc                             >  1:1251981/1‑150 (MQ=255)
                                                                                                                        gtCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCAc                             <  1:1099782/150‑1 (MQ=255)
                                                                                                                          ccTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACgc                           >  2:965254/1‑150 (MQ=255)
                                                                                                                          ccTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACgc                           <  2:948987/150‑1 (MQ=255)
                                                                                                                           ctctctTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACgct                          <  2:1464098/150‑1 (MQ=255)
                                                                                                                            tctctTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGAGGCGCAGGTGTTCGTTTCTGGCCGCGGGCAGATCAGAACGCTCAGGCTTACGCTGGATCGTACCTTCCTTCTTGCCAGCATCAACTTCCAGGCAGGGTGGGTGTTCCCggtg                         >  2:300695/1‑150 (MQ=255)
                                                                                                                                   ggTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagcc                  <  1:370301/150‑1 (MQ=255)
                                                                                                                                   ggTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagcc                  <  1:549916/150‑1 (MQ=255)
                                                                                                                                    gTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagcca                 >  2:11845/1‑150 (MQ=255)
                                                                                                                                      gtcgaGCTTTACTCCAAGTAAAGCTTGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGCTTGGCTTTTCACGCTGCTcagccagc               >  2:1517149/5‑150 (MQ=255)
                                                                                                                                      gtcgaGCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTcagccagc               >  1:1486085/5‑150 (MQ=255)
                                                                                                                                      aCTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTAAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGATGGCTTTTCACGCTGCTcagccagc               >  1:965942/1‑150 (MQ=255)
                                                                                                                                        cgaGCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTc             >  1:1694994/3‑150 (MQ=255)
                                                                                                                                         gaGCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCCGCCAGCTcc            >  2:355404/2‑150 (MQ=255)
                                                                                                                                         aaGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCCGCCAGCTcc            >  2:1300334/1‑150 (MQ=255)
                                                                                                                                         aaGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTcc            >  1:1170919/1‑150 (MQ=255)
                                                                                                                                         aaGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTcc            >  1:1722535/1‑150 (MQ=255)
                                                                                                                                          aGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTccc           >  2:799714/1‑149 (MQ=255)
                                                                                                                                          aGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCa           >  2:1602482/1‑150 (MQ=255)
                                                                                                                                          aGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGATCCGGCTTACGCTTAAACGTACGGTCGATCTTGCCCGCATCATCTTCCAGCGAGGTTGGCTTTTCACGCTGCTCAGCGAGCTCCa           >  2:243314/1‑150 (MQ=255)
                                                                                                                                          aGCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCa           >  2:1007923/1‑150 (MQ=255)
                                                                                                                                           gCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCCTCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCag          >  1:1042195/1‑150 (MQ=255)
                                                                                                                                           gCTTTACTCCAAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCag          >  2:1232029/1‑150 (MQ=255)
                                                                                                                                            cTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGACTTTTCACGCTGCTCAGCCAGCTCCaga         >  1:1534072/1‑150 (MQ=255)
                                                                                                                                              ttACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGc       >  2:1627650/1‑150 (MQ=255)
                                                                                                                                               tACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGATGCTCAGCCAGCTCCagagag      >  1:962755/1‑148 (MQ=255)
                                                                                                                                                aCTTGGAGTAAAGCTCGAAGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCgg     <  1:220137/150‑1 (MQ=255)
                                                                                                                                                 cTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCagagtgat    >  1:1649696/1‑146 (MQ=255)
                                                                                                                                                 cTTGGAGTAAAGCGCGACGAGCAGGAGTTCGTTAATGTCCCCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGc    <  2:727231/150‑1 (MQ=255)
                                                                                                                                                   tGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCtt  >  1:1380358/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCTTTTCACGCTGCTCAGCCAGCTCCAGAGCGGCTT  >  NC_000913/3440913‑3441209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑