BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	422,540	C→A	5.6%	P8Q (CCG→CAG)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,540	0	C	A	5.6%	334.4 / 7.1	115	P8Q (CCG→CAG)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base C (48/60); new base A (3/4); total (51/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 116 total aligned reads displayed.

TCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCG  >  NC_000913/422393‑422688

tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCACGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                                                                                                                                    <  2:250652/150‑1 (MQ=255)

 ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                                                                                                                                   <  1:1548608/150‑1 (MQ=255)

  cTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGTGCTCCTCTAGCAATTATAGTTCAGATTATGAGTGATCCTGCATTAGTCTATGGCTAGGGGATCTGTTTTTCATCACTGGGGAATTATGATGTTAAATGCATGGCAGCTGCCGGTg                                                                                                                                                  <  1:43200/150‑1 (MQ=255)

      tCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcccc                                                                                                                                              <  2:723125/150‑1 (MQ=255)

       ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCCGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatg                                                                                                                                             <  2:460934/150‑4 (MQ=255)

        cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                                                                                                                                            <  1:1170861/150‑1 (MQ=255)

         ggCTATTCCGAAAGTAATCCGCCCCCGTCCTCCTCACCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatgca                                                                                                                                           <  2:72200/150‑6 (MQ=255)

          gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                                                                                                                                          <  2:1345585/150‑1 (MQ=255)

           cTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatgcatt                                                                                                                                         <  1:646148/150‑8 (MQ=255)

               tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                                                                                                                                     <  1:605424/150‑1 (MQ=255)

               tCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAAAAAGCTACAGAAGAAGAGAGATCAGGCATTAGGGAATGTCATTGTGATGAGATTTTAAAAACATGTGAATTATGATTTTAAATTAATGTAACATAACGGTGGCCCCaaatgtat                                                                                                                                     >  2:1618266/1‑143 (MQ=255)

               tCCGAAAGTTATCCGCCCCCGGCCTCCGCCCGCAAAAATACTTCAGATGATGAATGCTACTGCATTAGGCGATGGCAAGGGGTAAAGATTTTGATATCAGGGGAGTAATGATGTTAAATGCATGGAACCATACCGTGCCCCCAGATGTTa                                                                                                                                     >  2:1624629/1‑150 (MQ=255)

                ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                                                                                                                                    <  1:1350014/150‑1 (MQ=255)

                ccGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCGTCAGATGATGAGTGATCCTGCATTAGGCTTTGGGTAGGTGGTCAGATTTTCATAACAGGGGAATTATGATGTTTAATGAATGGCACCTCCCGGTGCCCCCATTTGTTat                                                                                                                                    >  2:166365/1‑149 (MQ=255)

                 ctataGTTATCCGCCCCCGTCCTCCTGCCCCAAATATCCTTCACATGATGAGTGATCCTGCATTCGGCTATGGCAAGGTGAACAGATTTTCATACCAGGTGAATTATGATGTTAAATGCTTGGCACCTGCCGGTGCCCCCATTTGTTaaa                                                                                                                                   <  2:1081282/146‑1 (MQ=255)

                  gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                                                                                                                                  <  2:1598680/150‑1 (MQ=255)

                  gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                                                                                                                                  <  2:1521980/150‑1 (MQ=255)

                  gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                                                                                                                                  <  2:633392/150‑1 (MQ=255)

                      tttATCCGCCCCCGTCCTCCTCCCCAAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGGGCCCCCATTTGTTAAAcaaag                                                                                                                              <  2:193513/149‑1 (MQ=255)

                         aTCCGCCGCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGGATGGCAAGGTGAGCAGATTTTCAACAAAAGGGAATTATGATGTTAAATGCAATGCACCTGCCGGTGCCCCCAGGAGTTAAAcaaagcaa                                                                                                                           >  2:1128583/1‑150 (MQ=255)

                           ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag                                                                                                                         <  2:584012/150‑1 (MQ=255)

                              cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCCGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc                                                                                                                      <  2:1481258/150‑1 (MQ=255)

                              cccccGTCCTCCTCCCCCAAATATCATTCAGATGATGAGTGATCCTGCCTTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTTTTAAACAAAGCAAAGATc                                                                                                                      >  1:96714/1‑150 (MQ=255)

                              cccccGTCCTCCTCCCCCAAAAATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc                                                                                                                      >  2:970093/1‑150 (MQ=255)

                                cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCACTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa                                                                                                                    >  1:833478/1‑150 (MQ=255)

                                     cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATAACAGTGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAACCAAAGCAAAGATCAACTGCt                                                                                                               <  1:283242/150‑1 (MQ=255)

                                      ctcctccCCCAAATTTCCTTCAGATGATGAGTGATCCGGCATTCGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc                                                                                                              <  2:270871/150‑1 (MQ=255)

                                      ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACA‑GGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCa                                                                                                             <  2:124641/150‑1 (MQ=255)

                                           cccccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTac                                                                                                         <  1:1297789/150‑1 (MQ=255)

                                           cccccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTac                                                                                                         <  1:1218772/150‑1 (MQ=255)

                                            ccccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTaca                                                                                                        >  1:812429/1‑150 (MQ=255)

                                            ccccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTaca                                                                                                        >  2:1206333/1‑150 (MQ=255)

                                             cccAAATATCCTTCAGATGATGAGTGATCCTTCATTAGGCTATGGCAAGGTTATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTacac                                                                                                       <  2:1127558/150‑1 (MQ=255)

                                             cccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATATTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTacac                                                                                                       >  2:1094743/1‑150 (MQ=255)

                                             cccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGATATGGCAAGGTGATCATATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTacac                                                                                                       <  1:609537/150‑1 (MQ=255)

                                              ccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACt                                                                                                      <  2:1526313/150‑1 (MQ=255)

                                                 aaTATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTTATCAGATTTTCATCACAGGTTAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACtgtg                                                                                                   <  1:1607010/150‑1 (MQ=255)

                                                 aaTATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAGGGCAAGGTGATCAGATGTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCACCATATGTTAAACAAAGCAAAGATCCACTGCGAATTACACtggg                                                                                                   >  2:819809/1‑148 (MQ=255)

                                                  atatCCTTCAGATGATGAGTGATGCTGCATTTGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTgg                                                                                                  <  2:677606/150‑1 (MQ=255)

                                                  atatCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTgg                                                                                                  <  2:894319/150‑1 (MQ=255)

                                                  atatCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTCATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTgg                                                                                                  <  2:903258/150‑1 (MQ=255)

                                                       cTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGAc                                                                                             >  2:374205/1‑150 (MQ=255)

                                                       cTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGAc                                                                                             <  1:773188/150‑1 (MQ=255)

                                                          cAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACggg                                                                                          <  2:84227/150‑1 (MQ=255)

                                                          cAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACggg                                                                                          >  1:1265645/1‑150 (MQ=255)

                                                          cAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACggg                                                                                          <  2:1705701/150‑1 (MQ=255)

                                                          cAGATGATGACTGATCCTGCATTAGGATATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACggg                                                                                          <  1:277142/150‑1 (MQ=255)

                                                           aGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGc                                                                                         >  2:374929/1‑150 (MQ=255)

                                                            gatgatGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCACCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCg                                                                                        >  1:234599/1‑150 (MQ=255)

                                                            gatgagGACGGATCCAGCAGTAGCCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACACAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCg                                                                                        <  2:1130966/150‑1 (MQ=255)

                                                              tgatgaGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGaa                                                                                      >  2:62945/1‑150 (MQ=255)

                                                               gatgaGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAg                                                                                     <  1:970093/150‑1 (MQ=255)

                                                               gatgaGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAg                                                                                     <  1:362561/150‑1 (MQ=255)

                                                                 tgaGTGATCCTGCACTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGAc                                                                                   <  1:1398223/150‑1 (MQ=255)

                                                                      gATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCAcc                                                                              >  1:171689/1‑150 (MQ=255)

                                                                       aTCCTGCATTAGGCTATGGCAAGGTGATCAGATTTCCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCg                                                                             >  2:201602/1‑150 (MQ=255)

                                                                           tgccatAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAgc                                                                         <  1:142851/145‑1 (MQ=255)

                                                                            gCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAgcg                                                                        <  1:1544512/150‑1 (MQ=255)

                                                                              aTTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGAACCTGCCGGTGACCCCATTTGTTAAACAAAGAAAAGATCAACTGCTCATTACACTGTGGCTGAAGGGCGATGACCCACCGAAGCGCa                                                                      >  1:1558449/1‑150 (MQ=255)

                                                                                  ggCTATGGCAAGGTGATCAGATATTCATCACAGGGTAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGTCTGACGGGCGAATACCCACCGCAGCGCATTAt                                                                  <  1:639330/150‑1 (MQ=255)

                                                                                    cTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTAtgc                                                                >  2:1659185/1‑150 (MQ=255)

                                                                                      aTGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGCCCCACCGCAGCGCATTAtgctg                                                              >  2:676025/1‑150 (MQ=255)

                                                                                       tGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGCCGGGCGAAGACCCACCGCAGCGCATTAtgctgc                                                             >  2:250635/1‑150 (MQ=255)

                                                                                           aatttGATCAGATTTTCATCACAGGGTAATTATGATTTTAAATGAATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACTGGCGAAGACCCACCGCAGCGCATTATGCTGCGTAc                                                         <  1:1469278/146‑1 (MQ=255)

                                                                                             ggTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAg                                                       <  1:995669/150‑1 (MQ=255)

                                                                                             ggTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAg                                                       >  1:1659943/1‑150 (MQ=255)

                                                                                             ggTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACAGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAg                                                       <  1:1632282/150‑1 (MQ=255)

                                                                                                tatatgATTTTCATCA‑ACGGGTAATATGATGTTAAATGCATGTCACCTGCCGGTGCCCACATTTGTTAAACAAAGCAAATATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAaca                                                   <  2:376113/145‑1 (MQ=255)

                                                                                                gATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAac                                                    >  1:871028/1‑150 (MQ=255)

                                                                                                 aTCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAaca                                                   <  1:177809/150‑1 (MQ=255)

                                                                                                     gATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTAt                                                                  >  1:1663432/1‑131 (MQ=255)

                                                                                                     gATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTTCAGAACACGAt                                               >  1:800988/1‑150 (MQ=255)

                                                                                                       atttCATCACAGGCGAATAATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAATCAAAGCAAAGATGAACTGCTCATTACACTGTGGCTGACTGTCGATGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATaa                                             <  1:157553/149‑1 (MQ=255)

                                                                                                          tcatcaCAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAGGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACga                                          <  1:452744/150‑1 (MQ=255)

                                                                                                            atcaCAGGGTAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAGCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACgaag                                        <  2:440140/150‑1 (MQ=255)

                                                                                                            atcaCAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACgaag                                        >  2:937642/1‑150 (MQ=255)

                                                                                                                caGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAt                                    <  1:996139/150‑1 (MQ=255)

                                                                                                                caGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAt                                    >  2:896240/1‑150 (MQ=255)

                                                                                                                caGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAt                                    >  1:1606781/1‑150 (MQ=255)

                                                                                                                  ggggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGt                                  >  1:1550508/1‑150 (MQ=255)

                                                                                                                  ggggAATTATGATGTTAAATACATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCCCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGt                                  >  1:1434185/1‑150 (MQ=255)

                                                                                                                   gggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTc                                 >  1:1447513/1‑150 (MQ=255)

                                                                                                                     gAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGTCGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAg                               <  1:1258340/150‑1 (MQ=255)

                                                                                                                        ttATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTAc                            >  2:1391820/1‑150 (MQ=255)

                                                                                                                         tATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTAcc                           >  2:1002983/1‑150 (MQ=255)

                                                                                                                          atgatgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCg                          >  2:1613076/1‑150 (MQ=255)

                                                                                                                                 tAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATa                   <  1:1455348/150‑1 (MQ=255)

                                                                                                                                 tAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATa                   <  2:614745/150‑1 (MQ=255)

                                                                                                                                 tAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATa                   <  1:713152/150‑1 (MQ=255)

                                                                                                                                  aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATaa                  >  2:1005872/1‑150 (MQ=255)

                                                                                                                                    aTGCACGGCACCTGCCGGTGCCACCATTTGTTAAACAAAGCAACGATCAACTGCTCATTACACTGTTGCTTACGGGCGAAGACCCACCGCAGCGCACTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagc                <  2:708742/150‑1 (MQ=255)

                                                                                                                                       ggaggCACCGGCAGCTGCCCCCATTTCATAAACAAAGCAAAGATCAACTGCGCATTACACTGTGGCTGACGGTCCAAGTCCCAACCCAGCGCATTAAGATGCGTAAATAACACGAAACCGAATAAATGTACTTACAAATGCAAAagcagc             >  2:1386525/4‑150 (MQ=255)

                                                                                                                                       cATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagcagc             >  2:423569/1‑150 (MQ=255)

                                                                                                                                        ggggCACCGGCAGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAgcg            >  1:1512644/3‑150 (MQ=255)

                                                                                                                                        aTGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAgcg            >  2:806148/1‑150 (MQ=255)

                                                                                                                                          ggCACCTGCCGGTGCCCGCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCa          >  1:1124248/1‑150 (MQ=255)

                                                                                                                                              ccTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCa      >  2:79242/1‑150 (MQ=255)

                                                                                                                                              ccTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGAGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCa      <  1:293936/150‑1 (MQ=255)

                                                                                                                                                tGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGc    >  2:1650759/1‑150 (MQ=255)

                                                                                                                                                  ccGGTGCCCCCATTTGTTAAACAATGCAAAGATCACCTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCCGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCg  <  1:1609686/150‑1 (MQ=255)

TCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCG  >  NC_000913/422393‑422688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`