Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,470,106 C→T 7.8% intergenic (‑253/+39) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,470,1060CT 7.8% 450.6 / 18.0 153intergenic (‑253/+39)chiA/tufAperiplasmic endochitinase/translation elongation factor EF‑Tu 1
Reads supporting (aligned to +/- strand):  ref base C (73/67);  new base T (5/7);  total (79/74)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

Only 100 of 157 total aligned reads displayed.

ATAAGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCA  >  NC_000913/3469957‑3470254
                                                                                                                                                     |                                                                                                                                                    
aTAAGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGcc                                                                                                                                                      >  2:1033823/1‑150 (MQ=255)
aTAAGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGcc                                                                                                                                                      <  1:779103/150‑1 (MQ=255)
 tAAGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCg                                                                                                                                                     >  1:1356998/1‑150 (MQ=255)
  aaGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGa                                                                                                                                                    >  2:1035261/1‑150 (MQ=255)
   aGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCTTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGaa                                                                                                                                                   >  1:732197/1‑150 (MQ=255)
   aGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGaa                                                                                                                                                   >  2:749246/1‑150 (MQ=255)
        aaaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGTATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGcc                                                                                                                                              <  1:50791/150‑1 (MQ=255)
         aaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGccc                                                                                                                                             <  1:543753/150‑1 (MQ=255)
            ccTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCttt                                                                                                                                          <  1:742258/150‑1 (MQ=255)
              tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCttttt                                                                                                                                        <  1:129402/150‑2 (MQ=255)
              tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttc                                                                                                                                        <  2:1175381/150‑1 (MQ=255)
              tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttc                                                                                                                                        >  1:1724421/1‑150 (MQ=255)
              tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTGCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCttttt                                                                                                                                        <  1:1328552/150‑2 (MQ=255)
               taCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttca                                                                                                                                       <  1:182160/149‑1 (MQ=255)
               gACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttg                                                                                                                                       <  1:1248020/150‑3 (MQ=255)
                aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACGGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                      <  1:1624453/150‑4 (MQ=255)
                aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                      <  1:477608/150‑4 (MQ=255)
                aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                      <  1:1115340/150‑4 (MQ=255)
                 cAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaat                                                                                                                                     >  2:1362993/1‑150 (MQ=255)
                 cAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGGTCTGACAGTACGAAGAAGATATTCCGTCAAAAAATGCAAAAAGGTCGCCGTAGCGACCTTttcaat                                                                                                                                     >  2:273799/1‑150 (MQ=255)
                 aaaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgca                                                                                                                                     <  1:1477113/149‑5 (MQ=255)
                  aaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaatt                                                                                                                                    <  1:924372/150‑1 (MQ=255)
                   aaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaattc                                                                                                                                   >  2:1140025/1‑150 (MQ=255)
                   aaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaattc                                                                                                                                   >  2:568379/1‑150 (MQ=255)
                      taaGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaa                                                                                                                                <  1:387191/149‑1 (MQ=255)
                          cATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTa                                                                                                                            >  2:827924/1‑150 (MQ=255)
                           aTTTTAAAAACAGAAACATTCATATTTAATATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTAGTTCTGAAAGTACGAATAAGATATGCAGTCAACAAATGCAAAAAGGGCGACGAAGCGCCCTTTTCAATTCAAAACTaa                                                                                                                           >  1:1001532/1‑150 (MQ=255)
                           aTTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATTCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTaa                                                                                                                           <  1:539310/150‑1 (MQ=255)
                             tttAAAAACAGAAACATTCATATTTAAAAAGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTAGTTCAGACATTACGAATAAGATATGCCGTCAACAAATGCAAAAAAGGCGCCGAAGCGCCCTATTAAATTAAAAACTAAtt                                                                                                                         >  2:1620402/1‑150 (MQ=255)
                               tAAAAACAGAAACATTCATATTTAAAATTTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTaa                                                                                                                       <  1:1657085/150‑1 (MQ=255)
                                aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                      >  2:95295/1‑150 (MQ=255)
                                aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAAAAAATGAAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                      >  1:719874/1‑150 (MQ=255)
                                 aaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACg                                                                                                                     <  1:488690/150‑1 (MQ=255)
                                   aaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgtt                                                                                                                   >  1:402222/1‑149 (MQ=255)
                                   aaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgtg                                                                                                                   <  1:537325/150‑1 (MQ=255)
                                    aCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgtgt                                                                                                                  <  2:1079787/150‑1 (MQ=255)
                                     cAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGTATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTa                                                                                                                 >  2:664791/1‑150 (MQ=255)
                                       taaaCATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAAt                                                                                                               <  1:325115/149‑1 (MQ=255)
                                           cATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTAGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGc                                                                                                           >  1:1167256/1‑150 (MQ=255)
                                             ttCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                         <  1:1012931/150‑1 (MQ=255)
                                             ttCAGATTAAAAATGCTAAATTGAAGTGATAAAATAAAAAGGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                         <  2:81222/150‑1 (MQ=255)
                                                atatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGa                                                                                                      <  2:498921/150‑1 (MQ=255)
                                                 tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                     >  2:585820/1‑150 (MQ=255)
                                                 tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                     >  2:1418182/1‑150 (MQ=255)
                                                 tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                     >  2:331449/1‑150 (MQ=255)
                                                 tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                     <  1:52157/150‑1 (MQ=255)
                                                  atTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCTTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAAc                                                                                                    >  1:243629/1‑150 (MQ=255)
                                                       aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGTGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAg                                                                                               <  2:1660462/150‑1 (MQ=255)
                                                       aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAg                                                                                               <  2:1360402/150‑1 (MQ=255)
                                                        aaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGc                                                                                              <  2:688076/150‑1 (MQ=255)
                                                         aTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGca                                                                                             <  2:1349214/150‑1 (MQ=255)
                                                           gTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGcaac                                                                                           >  2:134691/1‑150 (MQ=255)
                                                              aaaTTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCaacaac                                                                                        >  2:1188105/1‑150 (MQ=255)
                                                                 ttgaattgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGcc                                                                                     <  2:1512809/150‑1 (MQ=255)
                                                                  tgaattgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGccc                                                                                    >  2:412383/1‑150 (MQ=255)
                                                                       tgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGcc                                                                               >  1:1701449/1‑150 (MQ=255)
                                                                           atTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACg                                                                           <  1:1582125/150‑1 (MQ=255)
                                                                                 aaTATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACgg                                                                     <  2:1392892/150‑1 (MQ=255)
                                                                                    atGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccg                                                                  <  1:423355/150‑1 (MQ=255)
                                                                                      gAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccgcc                                                                >  2:130927/1‑150 (MQ=255)
                                                                                          aatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAAATAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCa                                                            >  1:1045740/1‑150 (MQ=255)
                                                                                           atTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCTCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCAc                                                           <  1:279580/150‑1 (MQ=255)
                                                                                            tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACg                                                          <  2:233830/150‑1 (MQ=255)
                                                                                              tATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTCGCAACAACGCCCGCGCCAGCGGTACGGCCGCCTTCACggg                                                        >  2:1709501/1‑149 (MQ=255)
                                                                                              tATTCGTACTGTCAGAACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGa                                                        >  2:1694126/1‑150 (MQ=255)
                                                                                               aTTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAt                                                       >  2:438759/1‑150 (MQ=255)
                                                                                                ttCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAtt                                                      >  1:1318810/1‑150 (MQ=255)
                                                                                                  cGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGc                                                    <  1:664791/150‑1 (MQ=255)
                                                                                                  cGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTATAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGc                                                    >  1:99149/1‑150 (MQ=255)
                                                                                                   gTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAATGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCg                                                   <  2:531058/150‑1 (MQ=255)
                                                                                                       tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                               <  2:1724421/150‑1 (MQ=255)
                                                                                                       tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                               <  2:530776/150‑1 (MQ=255)
                                                                                                       tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                               >  1:216974/1‑150 (MQ=255)
                                                                                                       tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCACGCGCCAAAGGTACGGCCGCCATCACTGATTGCGaaaa                                               >  1:1337031/1‑149 (MQ=255)
                                                                                                        gACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACg                                              >  2:1224604/1‑150 (MQ=255)
                                                                                                          cAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCa                                            <  1:1696115/150‑1 (MQ=255)
                                                                                                             tACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGAc                                         <  1:1459422/150‑1 (MQ=255)
                                                                                                               cGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcg                                       >  1:1216545/1‑150 (MQ=255)
                                                                                                               cGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcg                                       >  2:1555450/1‑150 (MQ=255)
                                                                                                                gAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgt                                      <  2:748244/150‑1 (MQ=255)
                                                                                                                   tAAGATATGCCTTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTAAATTCAAAACTATTTAACGTGTAATTAGCCCAGAACTTTAGAATAAACGCCCGCGCCAACGGTACGGCCGCCTGCACGGATTGCGAAACGAAGACcgtcgt                                   <  1:447354/150‑1 (MQ=37)
                                                                                                                       atatGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCag                               >  1:354222/1‑149 (MQ=255)
                                                                                                                            ccGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAACCGCAGACCGTCGTCCATCGCGa                          >  1:316612/1‑150 (MQ=255)
                                                                                                                                caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTACTTAACGTGTAATTAGCCCAGAACTTTAGCATCAACGCCCGCGCCAACGGTTCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCgg                      >  1:1072539/1‑150 (MQ=255)
                                                                                                                                caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGAAGACCGTCGTCCATCGCGATCgg                      >  1:138570/1‑150 (MQ=255)
                                                                                                                                caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGAAATTATCCCAGAACTTTAGAAACAACGCCCGCGCCAACGGTAAGGACGCCGACACGGATTGCGAAACGCAGACCGTCGGCCATCGCGATCgg                      >  2:1140535/1‑150 (MQ=25)
                                                                                                                                 aacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCggg                     >  2:1123226/1‑150 (MQ=255)
                                                                                                                                 aacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCggg                     <  2:243629/150‑1 (MQ=255)
                                                                                                                                  acaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAACCTAATTAACGTGTAATTAGCCCAGAACTTTAGCATCAACGCCCGCGCCAACGGTCCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGt                    <  1:104043/150‑1 (MQ=255)
                                                                                                                                    aaaTGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTgg                  >  2:1301656/1‑150 (MQ=255)
                                                                                                                                     gaattgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTCCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCGTCGCGATCGGGTGGa                 >  2:154962/7‑150 (MQ=18)
                                                                                                                                       tGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATc               >  1:1533323/1‑150 (MQ=255)
                                                                                                                                        ttgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCa              >  1:886170/4‑150 (MQ=18)
                                                                                                                                         tgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCAAGAACTTTAGCAACAACGCCCGAGCCAACTGTACGGCCGCCTTCACGGATTGAGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAg             >  1:1271475/3‑150 (MQ=18)
                                                                                                                                          aaaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGCCCGTCGTCCATCGCGATCGGGTGGATCAgg            >  1:1177510/1‑150 (MQ=255)
                                                                                                                                           aaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAggg           <  1:1676175/150‑1 (MQ=255)
                                                                                                                                           aaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAggg           <  1:907942/150‑1 (MQ=255)
                                                                                                                                           aaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAggg           <  1:1418182/150‑1 (MQ=255)
                                                                                                                                            aaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACCGATTGACAAAATCAGCCCGTCAGCGAACGCCATCGGGTGCATCAggct          >  2:1116117/1‑148 (MQ=18)
                                                                                                                                            aaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGt          <  2:719400/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATAAGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCA  >  NC_000913/3469957‑3470254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: