Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,470,108 A→C 8.0% intergenic (‑255/+37) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,470,1080AC 8.0% 423.7 / 23.8 150intergenic (‑255/+37)chiA/tufAperiplasmic endochitinase/translation elongation factor EF‑Tu 1
Reads supporting (aligned to +/- strand):  ref base A (68/69);  new base C (5/7);  total (74/76)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-02

Only 100 of 154 total aligned reads displayed.

AAGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCATT  >  NC_000913/3469959‑3470256
                                                                                                                                                     |                                                                                                                                                     
aaGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGa                                                                                                                                                       >  2:1035261/1‑150 (MQ=255)
 aGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGaa                                                                                                                                                      >  2:749246/1‑150 (MQ=255)
  taaaaaaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAg                                                                                                                                                     <  1:412644/149‑1 (MQ=255)
      aaaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGTATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGcc                                                                                                                                                 <  1:50791/150‑1 (MQ=255)
       aaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGccc                                                                                                                                                <  1:543753/150‑1 (MQ=255)
          ccTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCTCttt                                                                                                                                             <  2:1377141/150‑1 (MQ=255)
          ccTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCttt                                                                                                                                             <  1:742258/150‑1 (MQ=255)
          ccTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCttt                                                                                                                                             >  2:1131322/1‑150 (MQ=255)
            tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTGCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCttttt                                                                                                                                           <  1:1328552/150‑2 (MQ=255)
            tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATACGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttc                                                                                                                                           <  1:53807/150‑1 (MQ=255)
             taCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttca                                                                                                                                          <  1:182160/149‑1 (MQ=255)
              aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACGGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                         <  1:1624453/150‑4 (MQ=255)
              aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                         <  1:477608/150‑4 (MQ=255)
              aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                         <  1:1115340/150‑4 (MQ=255)
               cAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaat                                                                                                                                        >  2:1362993/1‑150 (MQ=255)
               cAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGGTCTGACAGTACGAAGAAGATATTCCGTCAAAAAATGCAAAAAGGTCGCCGTAGCGACCTTttcaat                                                                                                                                        >  2:273799/1‑150 (MQ=255)
                aaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaatt                                                                                                                                       <  1:924372/150‑1 (MQ=255)
                aaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTttcaattc                                                                                                                                      >  2:365297/1‑150 (MQ=255)
                 aaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaattc                                                                                                                                      >  2:568379/1‑150 (MQ=255)
                    taaGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaa                                                                                                                                   <  1:387191/149‑1 (MQ=255)
                    gAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaa                                                                                                                                   >  2:1676175/1‑150 (MQ=255)
                     aaGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaaa                                                                                                                                  >  1:233830/1‑150 (MQ=255)
                      aGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAAc                                                                                                                                 <  2:257931/150‑1 (MQ=255)
                        cATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTa                                                                                                                               >  2:1696255/1‑150 (MQ=255)
                        cATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTa                                                                                                                               >  2:827924/1‑150 (MQ=255)
                         aTTTTAAAAACAGAAACATTCATATTTAATATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTAGTTCTGAAAGTACGAATAAGATATGCAGTCAACAAATGCAAAAAGGGCGACGAAGCGCCCTTTTCAATTCAAAACTaa                                                                                                                              >  1:1001532/1‑150 (MQ=255)
                             tAAAAACAGAAACATTCATATTTAAAATTTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTaa                                                                                                                         <  1:1657085/150‑1 (MQ=255)
                              aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                        <  2:1017136/150‑1 (MQ=255)
                              aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                        <  2:1577325/150‑1 (MQ=255)
                              aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                        >  2:95295/1‑150 (MQ=255)
                              aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAAAAAATGAAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                        >  1:719874/1‑150 (MQ=255)
                               aaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACg                                                                                                                       <  1:488690/150‑1 (MQ=255)
                                 aaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgtt                                                                                                                     >  1:402222/1‑149 (MQ=255)
                                 aaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgtg                                                                                                                     <  1:537325/150‑1 (MQ=255)
                                  aCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgtgt                                                                                                                    <  2:1079787/150‑1 (MQ=255)
                                   cAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGTATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTa                                                                                                                   >  2:664791/1‑150 (MQ=255)
                                     taaaCATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAAt                                                                                                                 <  1:325115/149‑1 (MQ=255)
                                       aaCATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGAAAGTACGAATAATATATGCAGTCAACAAATGCAAAAAGGGCGCCGAATCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTa                                                                                                               <  1:1376978/150‑1 (MQ=255)
                                         cATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGTCGCAGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGc                                                                                                             >  1:1231265/1‑150 (MQ=255)
                                         cATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTAGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGc                                                                                                             >  1:1167256/1‑150 (MQ=255)
                                           ttCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                           <  1:1012931/150‑1 (MQ=255)
                                           ttCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                           <  1:20479/150‑1 (MQ=255)
                                           ttCAGATTAAAAATGCTAAATTGAAGTGATAAAATAAAAAGGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                           <  2:81222/150‑1 (MQ=255)
                                            tCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTACACGTTAATTAgtttt                                                                                                          <  1:141995/150‑5 (MQ=255)
                                             aatatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATTATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAg                                                                                                         <  1:1608336/149‑1 (MQ=255)
                                              atatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGa                                                                                                        <  2:498921/150‑1 (MQ=255)
                                               tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                       <  1:52157/150‑1 (MQ=255)
                                               tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                       >  2:1418182/1‑150 (MQ=255)
                                                atTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCTTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAAc                                                                                                      >  1:243629/1‑150 (MQ=255)
                                                   tAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACttt                                                                                                   <  1:909625/150‑1 (MQ=255)
                                                     aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGTGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAg                                                                                                 <  2:1660462/150‑1 (MQ=255)
                                                     aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAg                                                                                                 >  1:849184/1‑150 (MQ=255)
                                                      aaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGc                                                                                                <  2:688076/150‑1 (MQ=255)
                                                       aTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGca                                                                                               <  2:1349214/150‑1 (MQ=255)
                                                       aTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGca                                                                                               <  2:1342522/150‑1 (MQ=255)
                                                            aaaTTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCaacaac                                                                                          >  2:1188105/1‑150 (MQ=255)
                                                                tgaattgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGccc                                                                                      >  2:412383/1‑150 (MQ=255)
                                                                   attgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCcgcg                                                                                   >  2:1408880/1‑150 (MQ=255)
                                                                   attgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCcgcc                                                                                   <  1:1724347/150‑2 (MQ=255)
                                                                     tgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGcc                                                                                 >  1:1701449/1‑150 (MQ=255)
                                                                     tgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGcc                                                                                 <  2:632000/150‑1 (MQ=255)
                                                                                    gAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccgcc                                                                  >  2:130927/1‑150 (MQ=255)
                                                                                      ataatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCtt                                                                >  1:731795/1‑150 (MQ=255)
                                                                                       taatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAAGAACGCCCGCGCCAACGGTACGGCCGCCTTc                                                               <  2:1083851/150‑1 (MQ=255)
                                                                                        aatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAAATAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCa                                                              >  1:1045740/1‑150 (MQ=255)
                                                                                        aatTTATTCGTTCTGACAGTACGAATAAGATAAGCCGTCAACAAATGCAAAAAGGTAGCCGAAGCTCCCTTATCAATTAAAAACTAATTAAAGTCTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGTTACGGCCGCCTTCa                                                              >  1:1478475/1‑150 (MQ=255)
                                                                                          tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACg                                                            <  1:1286430/150‑1 (MQ=255)
                                                                                          tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACg                                                            <  2:233830/150‑1 (MQ=255)
                                                                                           ttATTCGTACTGTCAGAACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACgg                                                           >  2:191002/1‑150 (MQ=255)
                                                                                            tATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTCGCAACAACGCCCGCGCCAGCGGTACGGCCGCCTTCACggg                                                          >  2:1709501/1‑149 (MQ=255)
                                                                                             aTTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAt                                                         >  2:438759/1‑150 (MQ=255)
                                                                                              ttCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAtt                                                        >  1:1318810/1‑150 (MQ=255)
                                                                                                    cTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAAGTGTAATTAGACCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGaaa                                                  >  1:559393/1‑150 (MQ=255)
                                                                                                     tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                                 <  2:530776/150‑1 (MQ=255)
                                                                                                     tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                                 >  2:551402/1‑150 (MQ=255)
                                                                                                     tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCACGCGCCAAAGGTACGGCCGCCATCACTGATTGCGaaaa                                                 >  1:1337031/1‑149 (MQ=255)
                                                                                                      gACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACg                                                >  2:1224604/1‑150 (MQ=255)
                                                                                                        cAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCa                                              <  1:1696115/150‑1 (MQ=255)
                                                                                                           tACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGAc                                           <  1:1459422/150‑1 (MQ=255)
                                                                                                             cGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcg                                         >  1:1216545/1‑150 (MQ=255)
                                                                                                              gAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgt                                        <  2:748244/150‑1 (MQ=255)
                                                                                                                 tAAGATATGCCTTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTAAATTCAAAACTATTTAACGTGTAATTAGCCCAGAACTTTAGAATAAACGCCCGCGCCAACGGTACGGCCGCCTGCACGGATTGCGAAACGAAGACcgtcgt                                     <  1:447354/150‑1 (MQ=37)
                                                                                                                   aGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTcc                                   >  2:1357683/1‑150 (MQ=255)
                                                                                                                     atatGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCag                                 >  1:354222/1‑149 (MQ=255)
                                                                                                                          ccGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAACCGCAGACCGTCGTCCATCGCGa                            >  1:316612/1‑150 (MQ=255)
                                                                                                                             tCAACAACTGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCg                         <  2:1538011/150‑1 (MQ=255)
                                                                                                                              caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTACTTAACGTGTAATTAGCCCAGAACTTTAGCATCAACGCCCGCGCCAACGGTTCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCgg                        >  1:1072539/1‑150 (MQ=255)
                                                                                                                              caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGAAGACCGTCGTCCATCGCGATCgg                        >  1:138570/1‑150 (MQ=255)
                                                                                                                              caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGAAATTATCCCAGAACTTTAGAAACAACGCCCGCGCCAACGGTAAGGACGCCGACACGGATTGCGAAACGCAGACCGTCGGCCATCGCGATCgg                        >  2:1140535/1‑150 (MQ=25)
                                                                                                                               aacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCggg                       >  2:1123226/1‑150 (MQ=255)
                                                                                                                                acaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAACCTAATTAACGTGTAATTAGCCCAGAACTTTAGCATCAACGCCCGCGCCAACGGTCCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGt                      <  1:104043/150‑1 (MQ=255)
                                                                                                                                   gaattgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTCCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCGTCGCGATCGGGTGGa                   >  2:154962/7‑150 (MQ=18)
                                                                                                                                     tGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATc                 >  1:1533323/1‑150 (MQ=255)
                                                                                                                                      ttgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCa                >  1:886170/4‑150 (MQ=18)
                                                                                                                                       tgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCAAGAACTTTAGCAACAACGCCCGAGCCAACTGTACGGCCGCCTTCACGGATTGAGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAg               >  1:1271475/3‑150 (MQ=18)
                                                                                                                                        gaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAgg              >  2:1258810/2‑150 (MQ=255)
                                                                                                                                        aaaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGCCCGTCGTCCATCGCGATCGGGTGGATCAgg              >  1:1177510/1‑150 (MQ=255)
                                                                                                                                         aaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAggg             <  1:1676175/150‑1 (MQ=255)
                                                                                                                                          aaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGt            <  2:719400/150‑1 (MQ=255)
                                                                                                                                                  ccGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCa    <  2:1167256/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCATT  >  NC_000913/3469959‑3470256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: