Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,470,109 A→G 8.0% intergenic (‑256/+36) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,470,1090AG 8.0% 429.4 / 19.3 150intergenic (‑256/+36)chiA/tufAperiplasmic endochitinase/translation elongation factor EF‑Tu 1
Reads supporting (aligned to +/- strand):  ref base A (69/69);  new base G (5/7);  total (74/76)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

AGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCATTTT  >  NC_000913/3469960‑3470258
                                                                                                                                                     |                                                                                                                                                      
aGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGaa                                                                                                                                                        >  2:749246/1‑150 (MQ=255)
 taaaaaaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAg                                                                                                                                                       <  1:412644/149‑1 (MQ=255)
         ccTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCTCttt                                                                                                                                               <  2:1377141/150‑1 (MQ=255)
         ccTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCttt                                                                                                                                               >  2:1131322/1‑150 (MQ=255)
         ccTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCttt                                                                                                                                               <  1:742258/150‑1 (MQ=255)
           tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttc                                                                                                                                             <  2:1175381/150‑1 (MQ=255)
           tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTGCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCttttt                                                                                                                                             <  1:1328552/150‑2 (MQ=255)
           tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATACGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttc                                                                                                                                             <  1:53807/150‑1 (MQ=255)
            taCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttca                                                                                                                                            <  1:182160/149‑1 (MQ=255)
             aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACGGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                           <  1:1624453/150‑4 (MQ=255)
             aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                           <  1:1115340/150‑4 (MQ=255)
             aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttttgc                                                                                                                                           <  1:477608/150‑4 (MQ=255)
              cAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGGTCTGACAGTACGAAGAAGATATTCCGTCAAAAAATGCAAAAAGGTCGCCGTAGCGACCTTttcaat                                                                                                                                          >  2:273799/1‑150 (MQ=255)
               aaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaatt                                                                                                                                         <  1:924372/150‑1 (MQ=255)
               aaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTttcaattc                                                                                                                                        >  2:365297/1‑150 (MQ=255)
                aaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaattc                                                                                                                                        >  2:568379/1‑150 (MQ=255)
                aaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaattc                                                                                                                                        >  2:1140025/1‑150 (MQ=255)
                   taaGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaa                                                                                                                                     <  1:387191/149‑1 (MQ=255)
                   gAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaa                                                                                                                                     >  2:1676175/1‑150 (MQ=255)
                    aaGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaaa                                                                                                                                    >  1:233830/1‑150 (MQ=255)
                       cATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTa                                                                                                                                 >  2:827924/1‑150 (MQ=255)
                        aTTTTAAAAACAGAAACATTCATATTTAATATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTAGTTCTGAAAGTACGAATAAGATATGCAGTCAACAAATGCAAAAAGGGCGACGAAGCGCCCTTTTCAATTCAAAACTaa                                                                                                                                >  1:1001532/1‑150 (MQ=255)
                        aTTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATTCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTaa                                                                                                                                <  1:539310/150‑1 (MQ=255)
                          tttAAAAACAGAAACATTCATATTTAAAAAGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTAGTTCAGACATTACGAATAAGATATGCCGTCAACAAATGCAAAAAAGGCGCCGAAGCGCCCTATTAAATTAAAAACTAAtt                                                                                                                              >  2:1620402/1‑150 (MQ=255)
                            tAAAAACAGAAACATTCATATTTAAAATTTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTaa                                                                                                                           <  1:1657085/150‑1 (MQ=255)
                             aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                          <  2:1017136/150‑1 (MQ=255)
                             aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                          >  2:95295/1‑150 (MQ=255)
                             aaaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAAAAAATGAAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                          >  1:719874/1‑150 (MQ=255)
                              aaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACg                                                                                                                         <  1:488690/150‑1 (MQ=255)
                              aaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTAAGAATAAGATATGCCTTCAACAAATGCAAAAAGGGCGCCGAAGATCACTTTTCAATTCAAAAATAATTAACg                                                                                                                         >  1:531058/1‑150 (MQ=255)
                                aaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgtt                                                                                                                       >  1:402222/1‑149 (MQ=255)
                                  cAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGTATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTa                                                                                                                     >  2:664791/1‑150 (MQ=255)
                                    taaaCATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAAt                                                                                                                   <  1:325115/149‑1 (MQ=255)
                                      aaCATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGAAAGTACGAATAATATATGCAGTCAACAAATGCAAAAAGGGCGCCGAATCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTa                                                                                                                 <  1:1376978/150‑1 (MQ=255)
                                        cATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGTCGCAGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGc                                                                                                               >  1:1231265/1‑150 (MQ=255)
                                        cATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTAGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGc                                                                                                               >  1:1167256/1‑150 (MQ=255)
                                          ttCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                             <  1:1012931/150‑1 (MQ=255)
                                          ttCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                             <  1:20479/150‑1 (MQ=255)
                                          ttCAGATTAAAAATGCTAAATTGAAGTGATAAAATAAAAAGGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                             <  2:81222/150‑1 (MQ=255)
                                           tCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTACACGTTAATTAgtttt                                                                                                            <  1:141995/150‑5 (MQ=255)
                                            aatatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATTATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAg                                                                                                           <  1:1608336/149‑1 (MQ=255)
                                             atatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGa                                                                                                          <  2:498921/150‑1 (MQ=255)
                                              tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                         <  1:52157/150‑1 (MQ=255)
                                              tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                         >  2:1418182/1‑150 (MQ=255)
                                               atTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCTTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAAc                                                                                                        >  1:243629/1‑150 (MQ=255)
                                                  tAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACttt                                                                                                     <  1:909625/150‑1 (MQ=255)
                                                    aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAg                                                                                                   <  2:1360402/150‑1 (MQ=255)
                                                    aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAg                                                                                                   >  1:849184/1‑150 (MQ=255)
                                                     aaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGc                                                                                                  <  2:688076/150‑1 (MQ=255)
                                                      aTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGca                                                                                                 <  2:1342522/150‑1 (MQ=255)
                                                        gTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGcaac                                                                                               >  2:134691/1‑150 (MQ=255)
                                                              ttgaattgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGcc                                                                                         <  2:1512809/150‑1 (MQ=255)
                                                               tgaattgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGccc                                                                                        >  2:412383/1‑150 (MQ=255)
                                                                  attgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCcgcc                                                                                     <  1:1724347/150‑2 (MQ=255)
                                                                    tgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGcc                                                                                   <  2:632000/150‑1 (MQ=255)
                                                                    tgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGcc                                                                                   >  1:1701449/1‑150 (MQ=255)
                                                                              aaTATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACgg                                                                         <  2:1392892/150‑1 (MQ=255)
                                                                                     ataatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCtt                                                                  >  1:731795/1‑150 (MQ=255)
                                                                                      taatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAAGAACGCCCGCGCCAACGGTACGGCCGCCTTc                                                                 <  2:1083851/150‑1 (MQ=255)
                                                                                       aatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAAATAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCa                                                                >  1:1045740/1‑150 (MQ=255)
                                                                                       aatTTATTCGTTCTGACAGTACGAATAAGATAAGCCGTCAACAAATGCAAAAAGGTAGCCGAAGCTCCCTTATCAATTAAAAACTAATTAAAGTCTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGTTACGGCCGCCTTCa                                                                >  1:1478475/1‑150 (MQ=255)
                                                                                         tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACg                                                              <  2:233830/150‑1 (MQ=255)
                                                                                         tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACg                                                              <  1:1286430/150‑1 (MQ=255)
                                                                                           tATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTCGCAACAACGCCCGCGCCAGCGGTACGGCCGCCTTCACggg                                                            >  2:1709501/1‑149 (MQ=255)
                                                                                           tATTCGTACTGTCAGAACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGa                                                            >  2:1694126/1‑150 (MQ=255)
                                                                                            aTTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAt                                                           >  2:438759/1‑150 (MQ=255)
                                                                                             ttCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAtt                                                          >  1:1318810/1‑150 (MQ=255)
                                                                                                   cTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAAGTGTAATTAGACCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGaaa                                                    >  1:559393/1‑150 (MQ=255)
                                                                                                    tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                                   <  2:530776/150‑1 (MQ=255)
                                                                                                    tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                                   >  2:551402/1‑150 (MQ=255)
                                                                                                    tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                                   <  2:1724421/150‑1 (MQ=255)
                                                                                                    tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCACGCGCCAAAGGTACGGCCGCCATCACTGATTGCGaaaa                                                   >  1:1337031/1‑149 (MQ=255)
                                                                                                       cAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCa                                                <  1:1696115/150‑1 (MQ=255)
                                                                                                          tACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGAc                                             <  1:1459422/150‑1 (MQ=255)
                                                                                                            cGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcg                                           >  1:1216545/1‑150 (MQ=255)
                                                                                                            cGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcg                                           >  2:1555450/1‑150 (MQ=255)
                                                                                                             gAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgt                                          <  2:748244/150‑1 (MQ=255)
                                                                                                                tAAGATATGCCTTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTAAATTCAAAACTATTTAACGTGTAATTAGCCCAGAACTTTAGAATAAACGCCCGCGCCAACGGTACGGCCGCCTGCACGGATTGCGAAACGAAGACcgtcgt                                       <  1:447354/150‑1 (MQ=37)
                                                                                                                  aGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTcc                                     >  2:1357683/1‑150 (MQ=255)
                                                                                                                    atatGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCag                                   >  1:354222/1‑149 (MQ=255)
                                                                                                                         ccGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAACCGCAGACCGTCGTCCATCGCGa                              >  1:316612/1‑150 (MQ=255)
                                                                                                                             caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTACTTAACGTGTAATTAGCCCAGAACTTTAGCATCAACGCCCGCGCCAACGGTTCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCgg                          >  1:1072539/1‑150 (MQ=255)
                                                                                                                             caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGAAGACCGTCGTCCATCGCGATCgg                          >  1:138570/1‑150 (MQ=255)
                                                                                                                              aacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCggg                         <  2:243629/150‑1 (MQ=255)
                                                                                                                               acaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAACCTAATTAACGTGTAATTAGCCCAGAACTTTAGCATCAACGCCCGCGCCAACGGTCCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGt                        <  1:104043/150‑1 (MQ=255)
                                                                                                                                gaaaTGCTAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTATCGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTg                       <  2:1071945/149‑1 (MQ=255)
                                                                                                                                 aaaTGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTgg                      >  2:1195390/1‑150 (MQ=255)
                                                                                                                                 aaaTGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTgg                      >  2:1301656/1‑150 (MQ=255)
                                                                                                                                  gaattgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTCCGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCGTCGCGATCGGGTGGa                     >  2:154962/7‑150 (MQ=18)
                                                                                                                                    tGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATc                   >  1:1533323/1‑150 (MQ=255)
                                                                                                                                     ttgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCa                  >  1:886170/4‑150 (MQ=18)
                                                                                                                                      tgaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCAAGAACTTTAGCAACAACGCCCGAGCCAACTGTACGGCCGCCTTCACGGATTGAGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAg                 >  1:1271475/3‑150 (MQ=18)
                                                                                                                                       gaaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAgg                >  2:1258810/2‑150 (MQ=255)
                                                                                                                                       aaaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGCCCGTCGTCCATCGCGATCGGGTGGATCAgg                >  1:1177510/1‑150 (MQ=255)
                                                                                                                                        aaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAggg               <  1:1676175/150‑1 (MQ=255)
                                                                                                                                         aaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACCGATTGACAAAATCAGCCCGTCAGCGAACGCCATCGGGTGCATCAggct              >  2:1116117/1‑148 (MQ=18)
                                                                                                                                         aaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGt              <  2:719400/150‑1 (MQ=255)
                                                                                                                                                 ccGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCa      <  2:1167256/150‑1 (MQ=255)
                                                                                                                                                     aGCTCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCAtttt  <  2:296164/150‑1 (MQ=255)
                                                                                                                                                     aGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCAtttt  <  1:568379/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCATTTT  >  NC_000913/3469960‑3470258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: