Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,361 T→G 5.3% intergenic (‑32/+39) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3610TG 5.3% 335.7 / 8.2 126intergenic (‑32/+39)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base T (53/65);  new base G (4/3);  total (58/68)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

Only 100 of 126 total aligned reads displayed.

GGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCA  >  NC_000913/3471213‑3471506
                                                                                                                                                     |                                                                                                                                                 
ggTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATACCTTACTATAGTGCTCTCCCCTTc                                                                                                                                                  >  1:929312/1‑150 (MQ=255)
   tttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGAGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                                                                                                               >  1:988618/1‑150 (MQ=255)
       gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCATTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                           >  1:1242729/1‑150 (MQ=255)
       gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTCTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                           >  1:740529/1‑150 (MQ=255)
       gCCAGTACGGTGGTGATTGAAGCGGGCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                           <  1:799569/150‑1 (MQ=255)
        ccAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagaga                                                                                                                                          >  1:1383493/1‑150 (MQ=255)
         cAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                                                                         >  2:102900/1‑150 (MQ=255)
         cAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTACagaagacgc                                                                                                                                         >  2:1580033/1‑147 (MQ=255)
             ccGGAGGTGCTTGCAGCGGTCAGAGGAGTTTTACCGTGGTCAACGTGGCCGAACGTACCAACGCTAACGTGCGGTTTGGTACGTTCAACTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCACTCCTGAAGGGGAGAGCAct                                                                                                                                     <  1:42876/149‑2 (MQ=255)
             aCGGGGGTGATTGCAGCGGGCAGAGTAGGTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACg                                                                                                                                     <  2:1525676/150‑1 (MQ=255)
              cGGTGGTGATTGCAGCGGTCAGTGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCATCGTTAACGTGCGGTTTTGTTCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                    <  2:367674/150‑1 (MQ=255)
              cGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                    >  1:554313/1‑150 (MQ=255)
                gtggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGa                                                                                                                                  >  2:1130584/1‑150 (MQ=255)
                    tgATTGCAGCGGTCAGAGGAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTATCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                                                              <  1:1010278/150‑1 (MQ=255)
                    ggcttGCAGCCGTCAGAGTCGTTTTACCGTGGTCCACGTGGCCGATCGTACCCACGTTAACCTGCGGGTTTGTACGTTCAAATTTTTCTTTAGACCCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                                                              <  2:19838/147‑1 (MQ=255)
                      aTTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                                                                                                            >  2:1361614/1‑150 (MQ=255)
                        tGCCGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTCCCAACGTTAAAGTGCGGTTTTGTACGTTACAATTTTTCTTTAGCCACGGCTATATTCCTTACTATAGAGCGCTCCCCTTCAGTCGTGAGGACGGGACTTAGGTa                                                                                                                          >  1:305328/1‑150 (MQ=255)
                          cAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACCCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                                                                        <  2:1479357/150‑1 (MQ=255)
                          cAGCGGTCAGAGATGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGCGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACAATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                                                                        <  1:193397/150‑1 (MQ=255)
                           aGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTa                                                                                                                       >  1:623370/1‑150 (MQ=255)
                              gggcAGAGTAGTTTTACCGGGGACAACGTGGCCGATAGAACCAACGTTAACGTGCGGTTTTGTACGTACAAATTTTTCTTTAGACACGGCTATATTCCTAACTATAGTGCTCCCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                                                                    <  1:371916/147‑1 (MQ=37)
                                 cAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                                                                                 <  2:1214947/150‑1 (MQ=255)
                                 cAGAGTAGTTTTACCGTGGCCAACGTGGCCGATAGTACAAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTAAGACACGGCTATATTCCTTACTATAGTGCGCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                                                                                 <  1:733542/150‑1 (MQ=255)
                                  agagTAGGTTTACCGTGGGCAACTTGGCCGATAGTACCAACGTAAACGTGCGGTTTTGTACGTTTAAATTATTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTACTAACCCTTa                                                                                                                <  1:902610/150‑1 (MQ=255)
                                   gagTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                                                                               >  2:388075/1‑150 (MQ=255)
                                    agtagtTTTACCGTGGTCAACGTGGCCTATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                                                              <  2:1636130/150‑1 (MQ=255)
                                       agtTTTACCGTGGTCAACTTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTAAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCtt                                                                                                           <  2:1663988/150‑1 (MQ=255)
                                        gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                          >  2:762560/1‑150 (MQ=255)
                                        gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                          >  2:645648/1‑150 (MQ=255)
                                          tttACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAtt                                                                                                        <  2:1127424/150‑1 (MQ=255)
                                           ttACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                                                                       <  2:263925/150‑1 (MQ=255)
                                             aCCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                                                                     >  1:156143/1‑150 (MQ=255)
                                             aCCGTGGGCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTAGTTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCGCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                                                                     <  1:495817/150‑1 (MQ=255)
                                              ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAcc                                                                                                    >  1:275525/1‑150 (MQ=255)
                                              ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCGTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGTTATTAACCCTTAGGCTTATTTAcc                                                                                                    >  2:119825/1‑150 (MQ=255)
                                                gTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                                                                  <  1:497273/150‑1 (MQ=255)
                                                  ggTCAACGTGGCCGATAGTACCAACGTAAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                                                                <  1:550317/150‑1 (MQ=255)
                                                   gTCAACGTGTCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg                                                                                               <  1:54530/150‑1 (MQ=255)
                                                   gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg                                                                                               >  1:813428/1‑150 (MQ=255)
                                                    tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                              >  2:1448900/1‑150 (MQ=255)
                                                    tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                              <  1:899379/150‑1 (MQ=255)
                                                    tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTGTTGTACGTTCAAATTTTTCTTTAGACACGGCTTTATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                              <  1:1190869/150‑1 (MQ=255)
                                                    tCAACATGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                              <  2:914934/150‑1 (MQ=255)
                                                     cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCt                                                                                             >  1:1616171/1‑150 (MQ=255)
                                                     cAACGTGGCCGATAGTACCAACGTTAACGTGCGGTATTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCATACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGCCTTATTTACCACGGGCt                                                                                             >  1:18524/1‑150 (MQ=255)
                                                      atcGTGGCCTAAACTACCACCTTTAACGTGCCGTTTTGAACGTCCAAATTTTTCTTAACACAGGCCAAAATACCTTACTATTGTCCTCACCCCTTCCGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCtt                                                                                            <  2:165318/148‑1 (MQ=255)
                                                        cGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                                                                          <  2:1204019/150‑1 (MQ=255)
                                                         gTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                                                                         >  1:436997/1‑150 (MQ=255)
                                                          tGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                                                                         >  2:659408/1‑150 (MQ=255)
                                                               gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                                                                   <  1:195439/150‑1 (MQ=255)
                                                               gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                                                                   <  1:297970/150‑1 (MQ=255)
                                                                aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                                                                  >  2:1624542/1‑150 (MQ=255)
                                                                 tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACTCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                                                                 <  2:929868/150‑1 (MQ=255)
                                                                 tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                                                                 <  2:504131/150‑1 (MQ=255)
                                                                    tACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACAACGGGCTTCAATTACGGCCTGa                                                                              >  1:1647304/1‑150 (MQ=255)
                                                                    tACCAACGTTAACGTGCGGATTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTATGCTTATTTACCACGGGCTTCAATTACGGCCTGa                                                                              >  2:1218310/1‑150 (MQ=255)
                                                                     aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCGATATTCCTTACAATAGAGCTCTCCCCATAATGAGAGAGCACTGGACTTTGGTATTAACCCATAGGCTTATTTACCAAGGGATTCAATTACGGCCTgcg                                                                             >  2:1047281/1‑148 (MQ=37)
                                                                      ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                                                                            <  1:1626745/150‑1 (MQ=255)
                                                                          cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                                                                        >  1:1223228/1‑150 (MQ=255)
                                                                              aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTAc                                                                    <  2:1616171/150‑1 (MQ=255)
                                                                              aaCGGGTGGTTTTGAACGTTAAAATTTTTCTTTACACCCGGCAATATTCCTTACTATTGTGCTCTCCCCTTCTGTATATAGAACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTAc                                                                    <  1:363115/150‑1 (MQ=255)
                                                                                    cGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGgcg                                                              >  2:1021735/1‑150 (MQ=255)
                                                                                      gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt                                                            >  2:186918/1‑150 (MQ=255)
                                                                                        tttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc                                                          >  1:1275413/1‑150 (MQ=255)
                                                                                         ttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca                                                         <  1:1000737/150‑1 (MQ=255)
                                                                                           gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGTGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                       <  1:843753/150‑1 (MQ=255)
                                                                                           gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCTCGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                       <  2:1242729/150‑1 (MQ=255)
                                                                                               gTTCAAATTTTGCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc                                                   <  2:481635/150‑1 (MQ=255)
                                                                                                    aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAg                                              <  2:1596674/150‑1 (MQ=255)
                                                                                                    aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCACTTCTGGAGAGAGCACGGGACTTTGGTATTAACCATTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAg                                              >  1:115408/1‑150 (MQ=255)
                                                                                                    aaTTTTTCTTTAGACACGGCTATATTCATTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAg                                              <  1:645648/150‑1 (MQ=255)
                                                                                                     aTTTTCCTTTCGACACGCCTGTATACCTTCCTCTCGGGGTCACCCCTTCTTGTCAGAGCACGGGACGTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAgg                                             <  2:185463/150‑1 (MQ=255)
                                                                                                        tttCTTAAGACACGGCTATATTCCTTACTATTGTGCTCTCCCCTTCAGGATAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAAt                                          <  1:1513900/150‑1 (MQ=255)
                                                                                                         ttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAAtt                                         >  1:1009502/1‑150 (MQ=255)
                                                                                                          tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTc                                        <  2:783325/150‑1 (MQ=255)
                                                                                                            tttAGACACGGCTATATTCCTTACTATAGCGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCa                                      <  1:1690069/150‑1 (MQ=255)
                                                                                                             ttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCAt                                     >  1:1265110/1‑150 (MQ=255)
                                                                                                             ttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCAt                                     >  1:530742/1‑150 (MQ=255)
                                                                                                             ttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACATCAGCAATTCCaa                                     >  2:1434881/1‑149 (MQ=255)
                                                                                                                 acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtg                                 <  1:102900/150‑1 (MQ=255)
                                                                                                                  cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtgt                                >  2:902126/1‑150 (MQ=255)
                                                                                                                  cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtgt                                >  1:385167/1‑150 (MQ=255)
                                                                                                                   acGGCTATATTCCTTACTATAGTGCGCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGTAATTCCATAGTGTa                               <  2:851381/150‑1 (MQ=255)
                                                                                                                      gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatga                            <  2:1107516/150‑1 (MQ=255)
                                                                                                                      gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatga                            >  1:1565996/1‑150 (MQ=255)
                                                                                                                      gCTATATTCCTTACAATAGTGCTCTCCCCTGCAGGAGAGAGCCCGGGACTGTGGTATTAACCCTTAGGCTTATTTACCACGGGATTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCCTAGTGTatga                            >  2:1478482/1‑150 (MQ=255)
                                                                                                                        tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatgatg                          <  2:554313/150‑1 (MQ=255)
                                                                                                                            ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACg                      <  2:275525/150‑1 (MQ=255)
                                                                                                                                ttACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCt                  <  1:1336019/150‑1 (MQ=255)
                                                                                                                                    tataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTgg              >  1:718227/1‑150 (MQ=255)
                                                                                                                                     ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGt             >  2:1718709/1‑150 (MQ=255)
                                                                                                                                      taGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc            <  2:618231/150‑1 (MQ=255)
                                                                                                                                      ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc            >  2:569169/3‑150 (MQ=255)
                                                                                                                                      ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc            >  1:1109267/3‑150 (MQ=255)
                                                                                                                                        ttGCTCACCCCTTCAGGAGAGAGCACGGGACTTTGGTAATAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCag          <  2:854963/149‑1 (MQ=255)
                                                                                                                                          gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCagag        <  2:662426/150‑1 (MQ=255)
                                                                                                                                          gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCagag        >  1:857980/1‑150 (MQ=255)
                                                                                                                                              tcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACg    <  2:1613717/150‑1 (MQ=255)
                                                                                                                                                ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGc   >  1:983584/1‑150 (MQ=255)
                                                                                                                                                 cccTTCAGGAGAGAGCACGGGACTTTGGTATTACCCCTTAGGCTAATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTCCTCGGCGCTTCATCATACTTCAGGAATTACATAGTGTATGAAGCACGACCTGTGGTCAGAGAACGCa  >  1:209743/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCA  >  NC_000913/3471213‑3471506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: