Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,471,366 A→G 5.0% intergenic (‑37/+34) tufA ← / ← fusA translation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,3660AG 5.0% 349.9 / 6.7 120intergenic (‑37/+34)tufA/fusAtranslation elongation factor EF‑Tu 1/protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base A (49/63);  new base G (3/3);  total (53/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

Only 100 of 126 total aligned reads displayed.

GCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGT  >  NC_000913/3471220‑3471513
                                                                                                                                                   |                                                                                                                                                   
gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTCTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                                                                                                                  >  1:740529/1‑150 (MQ=255)
  cAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGagagag                                                                                                                                                >  2:102900/1‑150 (MQ=255)
  cAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTACagaagacgc                                                                                                                                                >  2:1580033/1‑147 (MQ=255)
   aGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGc                                                                                                                                               <  1:779217/150‑1 (MQ=255)
     tACGGGGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCTCCTGAAGGGGAGAGCAc                                                                                                                                             <  1:1350478/150‑1 (MQ=21)
      ccGGAGGTGCTTGCAGCGGTCAGAGGAGTTTTACCGTGGTCAACGTGGCCGAACGTACCAACGCTAACGTGCGGTTTGGTACGTTCAACTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCACTCCTGAAGGGGAGAGCAct                                                                                                                                            <  1:42876/149‑2 (MQ=255)
      aCGGGGGTGATTGCAGCGGGCAGAGTAGGTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACg                                                                                                                                            <  2:1525676/150‑1 (MQ=255)
       cGGTGGTGATTGCAGCGGTCAGTGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCATCGTTAACGTGCGGTTTTGTTCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                           <  2:367674/150‑1 (MQ=255)
       cGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACgg                                                                                                                                           >  1:554313/1‑150 (MQ=255)
        ggtggtGATTGCAGCGGTCAGAGTAGATTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAAATTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACggg                                                                                                                                          <  1:1227232/150‑1 (MQ=255)
         gtggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGa                                                                                                                                         >  2:1130584/1‑150 (MQ=255)
             tgATTGCAGCGGTCAGAGGAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTATCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                                                                     <  1:1010278/150‑1 (MQ=255)
             ggcttGCAGCCGTCAGAGTCGTTTTACCGTGGTCCACGTGGCCGATCGTACCCACGTTAACCTGCGGGTTTGTACGTTCAAATTTTTCTTTAGACCCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACttt                                                                                                                                     <  2:19838/147‑1 (MQ=255)
                 tGCCGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTCCCAACGTTAAAGTGCGGTTTTGTACGTTACAATTTTTCTTTAGCCACGGCTATATTCCTTACTATAGAGCGCTCCCCTTCAGTCGTGAGGACGGGACTTAGGTa                                                                                                                                 >  1:305328/1‑150 (MQ=255)
                   cAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACCCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                                                                               <  2:1479357/150‑1 (MQ=255)
                   cAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                                                                               >  1:891285/1‑150 (MQ=255)
                   cAGCGGTCAGAGATGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGCGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACAATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAtt                                                                                                                               <  1:193397/150‑1 (MQ=255)
                    aGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTa                                                                                                                              >  1:623370/1‑150 (MQ=255)
                       gggcAGAGTAGTTTTACCGGGGACAACGTGGCCGATAGAACCAACGTTAACGTGCGGTTTTGTACGTACAAATTTTTCTTTAGACACGGCTATATTCCTAACTATAGTGCTCCCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                                                                                                                           <  1:371916/147‑1 (MQ=37)
                          cAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                                                                                        <  2:1214947/150‑1 (MQ=255)
                          cAGAGTAGTTTTACCGTGGCCAACGTGGCCGATAGTACAAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTAAGACACGGCTATATTCCTTACTATAGTGCGCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCtt                                                                                                                        <  1:733542/150‑1 (MQ=255)
                           agagTAGGTTTACCGTGGGCAACTTGGCCGATAGTACCAACGTAAACGTGCGGTTTTGTACGTTTAAATTATTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTACTAACCCTTa                                                                                                                       <  1:902610/150‑1 (MQ=255)
                            gagTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                                                                                                                      >  2:388075/1‑150 (MQ=255)
                             agtagtTTTACCGTGGTCAACGTGGCCTATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                                                                                                                     <  2:1636130/150‑1 (MQ=255)
                               tagtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCt                                                                                                                   <  1:952422/150‑1 (MQ=255)
                                agtTTTACCGTGGTCAACTTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTAAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCtt                                                                                                                  <  2:1663988/150‑1 (MQ=255)
                                 gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                                 >  2:645648/1‑150 (MQ=255)
                                 gtTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                                                                                                                 >  2:762560/1‑150 (MQ=255)
                                    ttACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAttt                                                                                                              <  2:263925/150‑1 (MQ=255)
                                      aCCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                                                                            >  1:156143/1‑150 (MQ=255)
                                      aCCGTGGGCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTAGTTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCGCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTAc                                                                                                            <  1:495817/150‑1 (MQ=255)
                                       ccGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCGTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGTTATTAACCCTTAGGCTTATTTAcc                                                                                                           >  2:119825/1‑150 (MQ=255)
                                         gTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCAc                                                                                                         <  1:497273/150‑1 (MQ=255)
                                           ggTCAACGTGGCCGATAGTACCAACGTAAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                                                                       <  1:550317/150‑1 (MQ=255)
                                           ggTCAACGTGGCAGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg                                                                                                       <  1:225056/150‑1 (MQ=255)
                                            gTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg                                                                                                      >  1:813428/1‑150 (MQ=255)
                                             tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                                     >  2:1448900/1‑150 (MQ=255)
                                             tCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTGTTGTACGTTCAAATTTTTCTTTAGACACGGCTTTATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                                     <  1:1190869/150‑1 (MQ=255)
                                             tCAACATGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGc                                                                                                     <  2:914934/150‑1 (MQ=255)
                                               atcGTGGCCTAAACTACCACCTTTAACGTGCCGTTTTGAACGTCCAAATTTTTCTTAACACAGGCCAAAATACCTTACTATTGTCCTCACCCCTTCCGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCtt                                                                                                   <  2:165318/148‑1 (MQ=255)
                                                 cGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa                                                                                                 <  2:1204019/150‑1 (MQ=255)
                                                   tGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa                                                                                                >  2:659408/1‑150 (MQ=255)
                                                        gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                                                                          <  1:297970/150‑1 (MQ=255)
                                                        gATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACgg                                                                                          <  1:195439/150‑1 (MQ=255)
                                                         aTAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGc                                                                                         >  2:1624542/1‑150 (MQ=255)
                                                          tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACTCGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                                                                        <  2:929868/150‑1 (MQ=255)
                                                          tAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGcc                                                                                        <  2:504131/150‑1 (MQ=255)
                                                             tACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACAACGGGCTTCAATTACGGCCTGa                                                                                     >  1:1647304/1‑150 (MQ=255)
                                                             tACCAACGTTAACGTGCGGATTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTATGCTTATTTACCACGGGCTTCAATTACGGCCTGa                                                                                     >  2:1218310/1‑150 (MQ=255)
                                                              aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAg                                                                                    >  2:1047277/1‑150 (MQ=255)
                                                              aCCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCGATATTCCTTACAATAGAGCTCTCCCCATAATGAGAGAGCACTGGACTTTGGTATTAACCCATAGGCTTATTTACCAAGGGATTCAATTACGGCCTgcg                                                                                    >  2:1047281/1‑148 (MQ=37)
                                                               ccAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGc                                                                                   <  1:1626745/150‑1 (MQ=255)
                                                                   cGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACg                                                                               >  1:1223228/1‑150 (MQ=255)
                                                                    tttAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGt                                                                              <  1:530837/149‑1 (MQ=255)
                                                                       aaCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTAc                                                                           <  2:1616171/150‑1 (MQ=255)
                                                                               gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt                                                                   >  2:186918/1‑150 (MQ=255)
                                                                               gTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCt                                                                   >  2:1233529/1‑150 (MQ=255)
                                                                                 tttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtc                                                                 >  1:1275413/1‑150 (MQ=255)
                                                                                  ttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtca                                                                <  1:1000737/150‑1 (MQ=255)
                                                                                   tttaccgtCAAATTTTTTATTAGCCACGGCTAATTTCCTTAAAAAAGGGCCCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcat                                                               <  1:1580033/143‑1 (MQ=255)
                                                                                    gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGTGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                              <  1:843753/150‑1 (MQ=255)
                                                                                    gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCTCGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                              <  2:1242729/150‑1 (MQ=255)
                                                                                    gTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTtcatc                                                              <  1:63349/150‑1 (MQ=255)
                                                                                        gTTCAAATTTTGCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATAc                                                          <  2:481635/150‑1 (MQ=255)
                                                                                             aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAg                                                     <  2:1596674/150‑1 (MQ=255)
                                                                                             aaTTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCACTTCTGGAGAGAGCACGGGACTTTGGTATTAACCATTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAg                                                     >  1:115408/1‑150 (MQ=255)
                                                                                              aTTTTCCTTTCGACACGCCTGTATACCTTCCTCTCGGGGTCACCCCTTCTTGTCAGAGCACGGGACGTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAgg                                                    <  2:185463/150‑1 (MQ=255)
                                                                                                 tttCTTAAGACACGGCTATATTCCTTACTATTGTGCTCTCCCCTTCAGGATAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAAt                                                 <  1:1513900/150‑1 (MQ=255)
                                                                                                  ttCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAAtt                                                >  1:1009502/1‑150 (MQ=255)
                                                                                                   tCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTc                                               <  2:783325/150‑1 (MQ=255)
                                                                                                     tttAGACACGGCTATATTCCTTACTATAGCGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCa                                             <  1:1690069/150‑1 (MQ=255)
                                                                                                      ttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCAt                                            <  1:431155/150‑1 (MQ=255)
                                                                                                      ttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCAt                                            >  1:1265110/1‑150 (MQ=255)
                                                                                                      ttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCAt                                            >  1:530742/1‑150 (MQ=255)
                                                                                                      ttAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACATCAGCAATTCCaa                                            >  2:1434881/1‑149 (MQ=255)
                                                                                                        aGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAg                                          >  2:145107/1‑150 (MQ=255)
                                                                                                          acacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtg                                        <  1:102900/150‑1 (MQ=255)
                                                                                                           cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtgt                                       >  1:385167/1‑150 (MQ=255)
                                                                                                           cacGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAgtgt                                       >  2:902126/1‑150 (MQ=255)
                                                                                                            acGGCTATATTCCTTACTATAGTGCGCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGTAATTCCATAGTGTa                                      <  2:851381/150‑1 (MQ=255)
                                                                                                               gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatga                                   <  2:1107516/150‑1 (MQ=255)
                                                                                                               gCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatga                                   >  1:1565996/1‑150 (MQ=255)
                                                                                                                 tataTTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTatgatg                                 <  2:554313/150‑1 (MQ=255)
                                                                                                                     ttCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACg                             <  2:275525/150‑1 (MQ=255)
                                                                                                                       ccTTACTATAGTGCTCTCCCCTTCAGGAGAGAGAACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAATACGGCCTGAGCCACGTTACTCGGCGCTTCATCATACGTCAGGCATTACATAGTGTAAGATTCACGAc                           >  1:315308/1‑150 (MQ=255)
                                                                                                                         ttACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCt                         <  1:1336019/150‑1 (MQ=255)
                                                                                                                             tataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTgg                     >  1:718227/1‑150 (MQ=255)
                                                                                                                              ataGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGt                    >  2:1718709/1‑150 (MQ=255)
                                                                                                                               taGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGGTGCACGACCTTTGGTc                   <  2:1147014/150‑1 (MQ=255)
                                                                                                                               taGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc                   <  2:618231/150‑1 (MQ=255)
                                                                                                                               ccgTGCTCTCTCCTGAAGGGGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTc                   >  2:569169/3‑150 (MQ=255)
                                                                                                                                 ttGCTCACCCCTTCAGGAGAGAGCACGGGACTTTGGTAATAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCag                 <  2:854963/149‑1 (MQ=255)
                                                                                                                                   gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCagag               <  2:662426/150‑1 (MQ=255)
                                                                                                                                   gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCagag               >  1:857980/1‑150 (MQ=255)
                                                                                                                                     tctcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGaa             >  1:1690686/1‑150 (MQ=255)
                                                                                                                                       tcCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACg           <  2:1613717/150‑1 (MQ=255)
                                                                                                                                         ccccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGc          >  1:983584/1‑150 (MQ=255)
                                                                                                                                          cccTTCAGGAGAGAGCACGGGACTTTGGTATTACCCCTTAGGCTAATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTCCTCGGCGCTTCATCATACTTCAGGAATTACATAGTGTATGAAGCACGACCTGTGGTCAGAGAACGCa         >  1:209743/1‑150 (MQ=255)
                                                                                                                                                aGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAg   >  2:1467964/1‑150 (MQ=255)
                                                                                                                                                 ggAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTAAGAGAACGCAGCTGAGt  >  2:1570338/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGT  >  NC_000913/3471220‑3471513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: