Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,483,242 G→C 6.0% S14T (AGC→ACC) ‡ yheT → UPF0017 family putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,483,2420GC 6.0% 347.9 / 9.7 113S14T (AGC→ACC) ‡yheTUPF0017 family putative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (47/58);  new base C (3/4);  total (50/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

Only 100 of 123 total aligned reads displayed.

GCCTGCAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTG  >  NC_000913/3483093‑3483391
                                                                                                                                                     |                                                                                                                                                     
gcctgcAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTcagcag                                                                                                                                                       >  2:857935/1‑150 (MQ=255)
  ctgcAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTcagcagca                                                                                                                                                     >  2:503714/1‑150 (MQ=255)
       aCAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCt                                                                                                                                                >  2:1227056/1‑150 (MQ=255)
        cAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAACTCAAATGATGACGAAGATAACGACGAACGATGCCAATTAATTCAGCAGCAGTGCTg                                                                                                                                               >  2:290017/1‑150 (MQ=255)
        cAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGCTGGCGCAGCGACCGCCGCCCGATGCCAATGAATTCAGCAGCAGTGCTg                                                                                                                                               >  1:831966/1‑150 (MQ=255)
        cAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTg                                                                                                                                               <  1:617357/150‑1 (MQ=255)
           cAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGTCCGATGCCAATGAATTCAGCACTGCTGCTGAAt                                                                                                                                            <  1:789499/150‑1 (MQ=255)
             aGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTc                                                                                                                                          >  2:1187115/1‑150 (MQ=255)
             aGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTc                                                                                                                                          <  1:330334/150‑1 (MQ=255)
              cccAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCa                                                                                                                                         <  1:148997/149‑1 (MQ=255)
                 aGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATcc                                                                                                                                      >  2:1133188/1‑150 (MQ=255)
                            cGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGc                                                                                                                           >  1:316572/1‑150 (MQ=255)
                               ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                        >  1:689167/1‑150 (MQ=255)
                               ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                        <  2:295286/150‑1 (MQ=255)
                               ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGAGGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                        <  2:1225337/150‑1 (MQ=255)
                                cTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTa                                                                                                                       >  2:571269/1‑150 (MQ=255)
                                 tGGAAGAGTGCGAAATGGCATGGCGGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCGGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAg                                                                                                                      <  2:967927/150‑1 (MQ=255)
                                  ggAAGAGTGCGAAATCGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGc                                                                                                                     <  1:1715210/150‑1 (MQ=255)
                                     agagTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAAt                                                                                                                  <  1:1191775/150‑1 (MQ=255)
                                       agggCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTg                                                                                                                <  2:598356/147‑1 (MQ=255)
                                          gCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGCTGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCa                                                                                                             >  1:1262341/1‑150 (MQ=255)
                                          gCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCa                                                                                                             >  1:1196176/1‑150 (MQ=255)
                                           cGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCAt                                                                                                            >  1:1688165/1‑150 (MQ=255)
                                            gAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCAAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATc                                                                                                           <  2:1431181/150‑1 (MQ=255)
                                             aaaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCt                                                                                                          <  1:398140/150‑1 (MQ=255)
                                             aaaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCt                                                                                                          >  2:517708/1‑150 (MQ=255)
                                             aaaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCt                                                                                                          <  1:587950/150‑1 (MQ=255)
                                              aaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTg                                                                                                         <  2:171965/150‑1 (MQ=255)
                                              aaTGGCATGGCTGCAAGCCCAGGTGCAGCTTGTGCAGATTCTGATGGAAGGCCAAAGCAAATGATGTAGAAGATAACGACGACCGTTGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTg                                                                                                         <  2:1079330/150‑1 (MQ=255)
                                               ttggcatggcGGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGc                                                                                                        <  2:143082/149‑1 (MQ=255)
                                               atggcatggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGc                                                                                                        <  1:981895/150‑1 (MQ=255)
                                               atggcatggcGGGAAGCGCAGGAGCAGCTGGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGc                                                                                                        <  2:123202/150‑1 (MQ=255)
                                                    atggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAAcc                                                                                                   >  1:101039/1‑150 (MQ=255)
                                                       gcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTTGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACAGATGCCAATGTATTCAGCAGCATTTCTGTATTCATCCCCATGCGCGGTTTTAGAAATTGTCATCTGCAAACCAtg                                                                                                <  2:160308/150‑1 (MQ=255)
                                                         tGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtgct                                                                                              <  1:637154/150‑1 (MQ=255)
                                                             aGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCcg                                                                                          >  2:1236551/1‑150 (MQ=255)
                                                                ccAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATACTGCCGCGt                                                                                       >  2:1290685/1‑150 (MQ=255)
                                                                 cAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTc                                                                                      <  2:1297505/150‑1 (MQ=255)
                                                                 cAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTc                                                                                      <  1:1283670/150‑1 (MQ=255)
                                                                 cAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGATGAATTCATCCCCATGCGCGGCTTAAGCAATTGTCATCTGCAAACCATGCTGCCGCGTc                                                                                      >  1:1443852/1‑150 (MQ=255)
                                                                  tggAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATTGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCt                                                                                     <  1:1500471/149‑1 (MQ=255)
                                                                   ggAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTg                                                                                    <  2:25910/150‑1 (MQ=255)
                                                                       cagcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtc                                                                                <  2:1603115/150‑1 (MQ=255)
                                                                         gcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcgt                                                                              >  2:1441468/1‑150 (MQ=255)
                                                                          cTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcgtc                                                                             <  2:746443/150‑1 (MQ=255)
                                                                              aGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCa                                                                         <  2:572730/150‑1 (MQ=255)
                                                                              aGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTAAGCAATTGACATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCa                                                                         >  2:727426/1‑150 (MQ=255)
                                                                                cAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAgg                                                                       <  1:1133188/150‑1 (MQ=255)
                                                                                  gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATTACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATCCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                     <  2:706851/150‑1 (MQ=255)
                                                                                  gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                     <  2:574304/150‑1 (MQ=255)
                                                                                  gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                     <  2:712995/150‑1 (MQ=255)
                                                                                    tgctgcTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGaa                                                                   <  1:324314/150‑1 (MQ=255)
                                                                                     gctgctGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCCTCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGaaa                                                                  >  1:1543380/1‑150 (MQ=255)
                                                                                      ctgctgGAAGGCCAAAGCTACTGATGGCGCAGATAAGGACGACCGATGCCAATGAATTCAGCAGCTGTGCTGAATTCATCCCCATGCGCTGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCTTCTGTTTCGTCGTCAGGTGAAAt                                                                 <  1:216218/150‑1 (MQ=255)
                                                                                        gctgGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTc                                                               <  1:230510/150‑1 (MQ=255)
                                                                                           ggAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAcc                                                            >  1:639944/1‑150 (MQ=255)
                                                                                            gAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAccc                                                           <  2:244640/150‑1 (MQ=255)
                                                                                            gAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCAGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAccc                                                           >  2:820278/1‑150 (MQ=255)
                                                                                              aGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCg                                                         >  2:1063773/1‑150 (MQ=255)
                                                                                                gCCAAAGCAACTGATGGCGCAGATAAGGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTa                                                       >  1:23227/1‑150 (MQ=255)
                                                                                                  cAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACt                                                     <  1:122626/150‑1 (MQ=255)
                                                                                                   aaaGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTg                                                    <  2:928485/150‑1 (MQ=255)
                                                                                                     aGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGc                                                  <  1:1677128/150‑1 (MQ=255)
                                                                                                     aGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGc                                                  <  1:517708/150‑1 (MQ=255)
                                                                                                        aaCTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGc                                               <  2:1475106/150‑1 (MQ=255)
                                                                                                               ggCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTCCTGGCAGCGGCTGGa                                        >  1:276911/1‑150 (MQ=255)
                                                                                                               ggCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGa                                        >  2:731929/1‑150 (MQ=255)
                                                                                                                gcgcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCGTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAg                                       <  1:844444/150‑1 (MQ=255)
                                                                                                                 cgcATAAAACGAAGTCCGATGCCCAATTATTCATCAGCCCGGCTGTATTCATCCCCCTGCGCGGCGTTTTCAATTGGCATCCGCCAACCCAGCCGGCGCGTCGGGTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGt                                      <  2:913937/150‑1 (MQ=255)
                                                                                                                     taTAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGcc                                  <  2:156688/149‑1 (MQ=255)
                                                                                                                     gATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGGCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGcc                                  <  1:929611/150‑1 (MQ=255)
                                                                                                                       tAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCg                                <  1:577167/150‑1 (MQ=255)
                                                                                                                        aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGa                               >  2:366067/1‑150 (MQ=255)
                                                                                                                        aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCACgc                               >  1:305800/1‑149 (MQ=255)
                                                                                                                        aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTAGCCCGa                               >  2:912440/1‑150 (MQ=255)
                                                                                                                        aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGAGGCTGGAGTTGCCCGa                               >  2:235712/1‑150 (MQ=255)
                                                                                                                        aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCATCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGa                               >  2:1715161/1‑150 (MQ=255)
                                                                                                                         acgacgACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGAc                              <  1:1045448/150‑1 (MQ=255)
                                                                                                                          cgacgaCCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCCTCCCCATGCGCGGCTTTAGCATTTGTCATCTGCAAACCATGCTGCCGAGTCTGTTTTGTCGTCAGTTGAAATTCACCCCGTACTGGCAGCGGCTTGAGTTGCCCGACg                             <  2:355951/150‑1 (MQ=255)
                                                                                                                          cgacgaCCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCACGTACTGGCAGCGGCTGGAGTTGCCCGACg                             >  1:467910/1‑150 (MQ=255)
                                                                                                                              gacCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGa                         <  2:1347323/150‑1 (MQ=255)
                                                                                                                                     gCCAATGAATTCATCAGCAGTGCTGAATCCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTc                  <  1:1396641/150‑1 (MQ=255)
                                                                                                                                      ccAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCTTGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCg                 <  2:838140/150‑1 (MQ=255)
                                                                                                                                      ccAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCg                 >  2:1338310/1‑150 (MQ=255)
                                                                                                                                       ggaTGAATTCAGCACTGCTGCTGAATTCATACCCATGCGCGGCTTTAGCAATTGTCATCTGATAACCATGATTCCGCGTGTGTTTCGTCGTCAGGTGAAATTCAACCCGTACTGGAAGCGGCTGTAGTTTCCGGACGGCGATTTTGtata                >  1:230656/3‑147 (MQ=255)
                                                                                                                                        gaTGAATTCAGCACTGCTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt               >  1:1342846/2‑150 (MQ=255)
                                                                                                                                        aaTGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAACCCATGCTGCCGCGTCTGTTTCGTAGTCAGGTTAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt               >  1:63115/1‑150 (MQ=255)
                                                                                                                                        aaTGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt               >  1:297248/1‑150 (MQ=255)
                                                                                                                                        aaTGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTAACACCGTACTGGCTGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt               >  2:489224/1‑150 (MQ=255)
                                                                                                                                          tGAATTCAGCACTGCTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGTAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCt             >  2:1126965/1‑150 (MQ=255)
                                                                                                                                           gAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCtt            <  1:1613862/150‑1 (MQ=255)
                                                                                                                                             aTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGc          >  1:753415/1‑150 (MQ=255)
                                                                                                                                             aTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGc          <  2:329028/150‑1 (MQ=255)
                                                                                                                                             aTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTAGCCCGACGGCGATTTTGTCGATCTTGc          >  1:425571/1‑150 (MQ=255)
                                                                                                                                                cagcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTg       >  2:402289/1‑150 (MQ=255)
                                                                                                                                                   cagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCCGCGGCTGGAGTTGCCCGACGGCGAGTTTGTCGATCTTGCGTGGAg    >  1:718733/1‑150 (MQ=255)
                                                                                                                                                   cagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAg    >  1:725159/1‑150 (MQ=255)
                                                                                                                                                    agcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGt   <  2:316572/150‑1 (MQ=255)
                                                                                                                                                    agcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGt   >  2:843166/1‑150 (MQ=255)
                                                                                                                                                     gcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTg  >  1:533334/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTGCAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTG  >  NC_000913/3483093‑3483391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: