Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,483,245 G→C 6.7% S15T (AGT→ACT) ‡ yheT → UPF0017 family putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,483,2450GC 6.7% 358.0 / 10.4 113S15T (AGT→ACT) ‡yheTUPF0017 family putative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (47/57);  new base C (3/5);  total (50/63)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 123 total aligned reads displayed.

TGCAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGAAA  >  NC_000913/3483096‑3483394
                                                                                                                                                     |                                                                                                                                                     
tgcAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTcagcagcag                                                                                                                                                       >  1:1213670/1‑150 (MQ=255)
    aCAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCt                                                                                                                                                   >  2:1227056/1‑150 (MQ=255)
     cAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAACTCAAATGATGACGAAGATAACGACGAACGATGCCAATTAATTCAGCAGCAGTGCTg                                                                                                                                                  >  2:290017/1‑150 (MQ=255)
     cAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGCTGGCGCAGCGACCGCCGCCCGATGCCAATGAATTCAGCAGCAGTGCTg                                                                                                                                                  >  1:831966/1‑150 (MQ=255)
     cAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTg                                                                                                                                                  <  1:617357/150‑1 (MQ=255)
       gCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGaa                                                                                                                                                >  1:1603115/1‑150 (MQ=255)
       gCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGAAACTGATGGCGCAGATAACGCCGACCGATGCCAATGAATTCAGCAGCAGTGCTGaa                                                                                                                                                >  1:500267/1‑150 (MQ=255)
          aGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTc                                                                                                                                             <  1:330334/150‑1 (MQ=255)
          aGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTc                                                                                                                                             >  2:1187115/1‑150 (MQ=255)
           cccAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCa                                                                                                                                            <  1:148997/149‑1 (MQ=255)
              aGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCAttg                                                                                                                                         <  2:291294/150‑3 (MQ=255)
              aGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCACTGCTGCTGAATTCAttg                                                                                                                                         <  2:389134/150‑3 (MQ=255)
                   cAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATg                                                                                                                                    <  1:595785/150‑1 (MQ=255)
                         cGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGc                                                                                                                              >  1:316572/1‑150 (MQ=255)
                            ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                           >  1:791925/1‑150 (MQ=255)
                            ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                           <  2:295286/150‑1 (MQ=255)
                            ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGAGGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                           <  2:1225337/150‑1 (MQ=255)
                            ccTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAACAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCttt                                                                                                                           <  2:1726195/150‑1 (MQ=255)
                             cTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTa                                                                                                                          >  2:571269/1‑150 (MQ=255)
                              tGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAg                                                                                                                         <  2:1062877/150‑1 (MQ=255)
                              tGGAAGAGTGCGAAATGGCATGGCGGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCGGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAg                                                                                                                         <  2:967927/150‑1 (MQ=255)
                                  agagTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAAt                                                                                                                     <  1:1191775/150‑1 (MQ=255)
                                       gCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGCTGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCa                                                                                                                >  1:1262341/1‑150 (MQ=255)
                                        cGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCAt                                                                                                               >  1:1688165/1‑150 (MQ=255)
                                        cGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCAt                                                                                                               >  2:54932/1‑150 (MQ=255)
                                         gAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCAAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATc                                                                                                              <  2:1431181/150‑1 (MQ=255)
                                          aaaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCt                                                                                                             <  1:398140/150‑1 (MQ=255)
                                           aaTGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTg                                                                                                            <  2:171965/150‑1 (MQ=255)
                                           aaTGGCATGGCTGCAAGCCCAGGTGCAGCTTGTGCAGATTCTGATGGAAGGCCAAAGCAAATGATGTAGAAGATAACGACGACCGTTGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTg                                                                                                            <  2:1079330/150‑1 (MQ=255)
                                            ttggcatggcGGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGc                                                                                                           <  2:143082/149‑1 (MQ=255)
                                            atggcatggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGc                                                                                                           <  1:981895/150‑1 (MQ=255)
                                            atggcatggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCGGGAAGGCCAAAGCAACTGATGGTGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGc                                                                                                           <  1:713431/150‑1 (MQ=255)
                                                 atggcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAAcc                                                                                                      >  1:101039/1‑150 (MQ=255)
                                                    gcTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTTGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACAGATGCCAATGTATTCAGCAGCATTTCTGTATTCATCCCCATGCGCGGTTTTAGAAATTGTCATCTGCAAACCAtg                                                                                                   <  2:160308/150‑1 (MQ=255)
                                                      tGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCAtgct                                                                                                 <  1:637154/150‑1 (MQ=255)
                                                          aGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCcg                                                                                             >  2:1236551/1‑150 (MQ=255)
                                                             ccAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATACTGCCGCGt                                                                                          >  2:1290685/1‑150 (MQ=255)
                                                              cAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTc                                                                                         <  2:1297505/150‑1 (MQ=255)
                                                              cAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTc                                                                                         <  1:1283670/150‑1 (MQ=255)
                                                              cAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGATGAATTCATCCCCATGCGCGGCTTAAGCAATTGTCATCTGCAAACCATGCTGCCGCGTc                                                                                         >  1:1443852/1‑150 (MQ=255)
                                                               tggAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATTGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCt                                                                                        <  1:1500471/149‑1 (MQ=255)
                                                                    cagcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtc                                                                                   <  2:1603115/150‑1 (MQ=255)
                                                                      gcTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTtcgt                                                                                 >  2:1441468/1‑150 (MQ=255)
                                                                           aGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCa                                                                            <  2:572730/150‑1 (MQ=255)
                                                                           aGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTAAGCAATTGACATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCa                                                                            >  2:727426/1‑150 (MQ=255)
                                                                             cAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAgg                                                                          <  1:1133188/150‑1 (MQ=255)
                                                                               gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATTACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATCCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                        <  2:706851/150‑1 (MQ=255)
                                                                               gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                        <  2:574304/150‑1 (MQ=255)
                                                                               gATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTg                                                                        <  2:712995/150‑1 (MQ=255)
                                                                                  gctgctGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGaaa                                                                     >  2:1287942/1‑150 (MQ=255)
                                                                                   ctgctgGAAGGCCAAAGCTACTGATGGCGCAGATAAGGACGACCGATGCCAATGAATTCAGCAGCTGTGCTGAATTCATCCCCATGCGCTGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCTTCTGTTTCGTCGTCAGGTGAAAt                                                                    <  1:216218/150‑1 (MQ=255)
                                                                                     gctgGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTc                                                                  <  1:230510/150‑1 (MQ=255)
                                                                                        ggAAGGCCAAAGCAACTGATGGCTCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAcc                                                               <  2:1592756/150‑1 (MQ=255)
                                                                                        ggAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTcccc                                                               >  1:329028/1‑150 (MQ=255)
                                                                                        ggAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAcc                                                               >  1:639944/1‑150 (MQ=255)
                                                                                        ggAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAcc                                                               <  1:18570/150‑1 (MQ=255)
                                                                                         gAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAccc                                                              <  2:244640/150‑1 (MQ=255)
                                                                                         gAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCAGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCAccc                                                              >  2:820278/1‑150 (MQ=255)
                                                                                           aGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCg                                                            >  2:1063773/1‑150 (MQ=255)
                                                                                               cAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACt                                                        <  1:122626/150‑1 (MQ=255)
                                                                                                aaaGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTg                                                       <  2:928485/150‑1 (MQ=255)
                                                                                                  aGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGc                                                     <  1:1677128/150‑1 (MQ=255)
                                                                                                  aGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGc                                                     <  1:517708/150‑1 (MQ=255)
                                                                                                      aCTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCg                                                 >  1:1360031/1‑150 (MQ=255)
                                                                                                         gATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCAGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCt                                              >  2:1389044/1‑150 (MQ=255)
                                                                                                            ggCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTCCTGGCAGCGGCTGGa                                           >  1:276911/1‑150 (MQ=255)
                                                                                                            ggCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGa                                           >  2:731929/1‑150 (MQ=255)
                                                                                                             gcgcAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCGTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAg                                          <  1:844444/150‑1 (MQ=255)
                                                                                                              cgcATAAAACGAAGTCCGATGCCCAATTATTCATCAGCCCGGCTGTATTCATCCCCCTGCGCGGCGTTTTCAATTGGCATCCGCCAACCCAGCCGGCGCGTCGGGTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGt                                         <  2:913937/150‑1 (MQ=255)
                                                                                                                 aGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGc                                      <  2:79426/150‑1 (MQ=255)
                                                                                                                  taTAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGcc                                     <  2:156688/149‑1 (MQ=255)
                                                                                                                    tAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCg                                   <  1:577167/150‑1 (MQ=255)
                                                                                                                     aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGa                                  >  2:366067/1‑150 (MQ=255)
                                                                                                                     aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCACgc                                  >  1:305800/1‑149 (MQ=255)
                                                                                                                     aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTAGCCCGa                                  >  2:912440/1‑150 (MQ=255)
                                                                                                                     aaCGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGAGGCTGGAGTTGCCCGa                                  >  2:235712/1‑150 (MQ=255)
                                                                                                                      acgacgACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGAc                                 <  1:1045448/150‑1 (MQ=255)
                                                                                                                         acgacCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGc                              >  2:1211036/1‑150 (MQ=255)
                                                                                                                           gacCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGa                            <  2:1347323/150‑1 (MQ=255)
                                                                                                                            acagATGACACTCAATTCAGAATCAGTGCTGAATTCATCCCCATGAGCGGCTGTATCAATTGTCATCTGCAAACCATGCTGCCTCGTCTGTTACGTCGTCAGGTGAAAAGCACCCCGTCCTGGCAGCGGCTGGCGTTGCCCGACGGCGAt                           <  1:251907/147‑1 (MQ=255)
                                                                                                                                  gCCAATGAATTCATCAGCAGTGCTGAATCCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTc                     <  1:1396641/150‑1 (MQ=255)
                                                                                                                                   ccAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCTTGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCg                    <  2:838140/150‑1 (MQ=255)
                                                                                                                                    ggaTGAATTCAGCACTGCTGCTGAATTCATACCCATGCGCGGCTTTAGCAATTGTCATCTGATAACCATGATTCCGCGTGTGTTTCGTCGTCAGGTGAAATTCAACCCGTACTGGAAGCGGCTGTAGTTTCCGGACGGCGATTTTGtata                   >  1:230656/3‑147 (MQ=255)
                                                                                                                                     aaTGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAACCCATGCTGCCGCGTCTGTTTCGTAGTCAGGTTAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt                  >  1:63115/1‑150 (MQ=255)
                                                                                                                                     aaTGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt                  >  1:297248/1‑150 (MQ=255)
                                                                                                                                     aaTGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTAACACCGTACTGGCTGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGAt                  >  2:489224/1‑150 (MQ=255)
                                                                                                                                       tGAATTCAGCACTGCTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGTAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCt                >  2:1126965/1‑150 (MQ=255)
                                                                                                                                        gAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCtt               <  1:1613862/150‑1 (MQ=255)
                                                                                                                                          aTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGc             <  2:329028/150‑1 (MQ=255)
                                                                                                                                          aTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGc             >  1:753415/1‑150 (MQ=255)
                                                                                                                                          aTTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTAGCCCGACGGCGATTTTGTCGATCTTGc             >  1:425571/1‑150 (MQ=255)
                                                                                                                                             cagcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTg          <  2:689167/150‑1 (MQ=255)
                                                                                                                                             cagcagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTg          >  2:402289/1‑150 (MQ=255)
                                                                                                                                                cagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCCGCGGCTGGAGTTGCCCGACGGCGAGTTTGTCGATCTTGCGTGGAg       >  1:718733/1‑150 (MQ=255)
                                                                                                                                                cagcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAg       >  1:725159/1‑150 (MQ=255)
                                                                                                                                                 agcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGt      <  2:316572/150‑1 (MQ=255)
                                                                                                                                                 agcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGt      >  2:843166/1‑150 (MQ=255)
                                                                                                                                                  gcagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTg     >  1:533334/1‑150 (MQ=255)
                                                                                                                                                   cagTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGa    <  1:857935/150‑1 (MQ=255)
                                                                                                                                                     gTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGGCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGaaa  <  1:1063773/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCAACAGCAAGCCAGCGCCAAATCCGGCCTGGAAGAGTGCGAAATGGCATGGCTGGAAGCCCAGGAGCAGCTTGAGCAGATGCTGCTGGAAGGCCAAAGCAACTGATGGCGCAGATAACGACGACCGATGCCAATGAATTCAGCAGCAGTGCTGAATTCATCCCCATGCGCGGCTTTAGCAATTGTCATCTGCAAACCATGCTGCCGCGTCTGTTTCGTCGTCAGGTGAAATTCACCCCGTACTGGCAGCGGCTGGAGTTGCCCGACGGCGATTTTGTCGATCTTGCGTGGAGTGAAA  >  NC_000913/3483096‑3483394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: