Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,491,704 | G→T | 7.3% | intergenic (‑84/+21) | yhfG ← / ← ppiA | putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A) |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,491,704 | 0 | G | T | 7.3% | 235.3 / 6.3 | 94 | intergenic (‑84/+21) | yhfG/ppiA | putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A) |
Reads supporting (aligned to +/- strand): ref base G (24/61); new base T (3/3); total (30/64) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01 |
CTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA > NC_000913/3491555‑3491852 | cTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAg < 1:1139384/150‑1 (MQ=255) aaaTTAAGCTTAAGCGGAATCTCCCAGCTACGGGTCTTTTGCTTATCGGTGTTGTTCTTCAATTGATTCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTg < 2:1032276/150‑1 (MQ=255) aaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCAC‑GGGCAAGTTTGc > 2:102927/1‑150 (MQ=255) tacgattacgCTGAAGCTCCCAGAGCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCcg < 1:1695495/150‑1 (MQ=255) cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCcgcg > 2:207102/1‑150 (MQ=255) tattacgCTGAAGGTCCCAGAGACGGGACTTTTGCTTATCGTGGAGTTTCTTCACTTGATGCGTCCCTGAACGTGTCCATTTGCCACTAGTATAATCGGCAAATCCGGGTTACGGCGGGAGAGCTGAAGCGC‑GGGCAATCTTGCCcgcgc < 2:1327153/149‑1 (MQ=255) attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCTC‑CGGCAAAATTTGCcccgcg > 2:494280/1‑150 (MQ=255) attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAATCTTGCCcgcgct < 1:1683220/150‑2 (MQ=255) attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCAATTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAATCTTGCCcgcgct < 1:843700/150‑2 (MQ=255) ttacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCcgcgcgg > 1:900221/1‑149 (MQ=255) acgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCgaga < 1:494280/150‑1 (MQ=255) gCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGGCAGAATGGCCGCGCGAGtaa > 1:1068758/1‑150 (MQ=255) cgCTCCCAGAGCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCAtt < 1:1080323/149‑1 (MQ=255) aGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCAtt < 1:92439/150‑1 (MQ=255) cTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGG‑GGGGCAGATTTACCCCCCGAGaaaaaaaata > 1:1396728/1‑143 (MQ=255) cccAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAACTACGGGTTACTCCGGGAGAGCAGAAGCGC‑GGGCCAGATTGCCCGCGCGAGCACTCATTACgg > 2:93229/1‑150 (MQ=255) agaCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGACAAAACAATAAGGCAGGa > 2:215901/1‑150 (MQ=255) aCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACt < 2:338767/150‑1 (MQ=255) gggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACttt < 1:1314181/150‑1 (MQ=255) gACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAg < 1:1034508/150‑1 (MQ=255) aCTTTTCCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGGGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGc < 1:1376491/150‑1 (MQ=255) ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg < 2:1222359/150‑1 (MQ=255) ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGGGTCAATTTGCCCCACGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg < 2:1463533/150‑1 (MQ=255) ttttGCTTATCGGAGAGTTTCTTCACTTGATGCCTCCCTGACCGTGTCCATTTGCCACAATTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGAGTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg < 2:867734/150‑1 (MQ=255) tttGCTTCTCTGGGAGTTTCTTCACTTTATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCTGCAACTCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGa < 2:906441/150‑1 (MQ=255) cTTCTCGGGGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCCCAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAgg < 2:1126867/150‑1 (MQ=255) tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGAt < 1:431442/150‑1 (MQ=255) aTCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAGTCATTACGGCAGGACTTTAGCGGAAAGGATa < 2:28792/150‑1 (MQ=255) tCGGTGAGTTTCTTCACTTGATGCCTCCCTGACCGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATaa < 1:543567/150‑1 (MQ=255) ggTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCCCAAGTATAAGAGGCAAATCCGGGTTACGCCGGGTGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACt < 1:333948/150‑1 (MQ=255) gAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑c‑c < 2:509595/150‑1 (MQ=255) gTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGGAACAATGGCCCCCCGAGAAAAAAATACCGCACGTCGTTATCGGATAGGATAACtcaccg > 2:707004/1‑145 (MQ=255) ttcttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCCAGCTTTGCCCCGCCGCAAAACAATACCGCAGGGCGTGTGAGGCTACGATAACTA‑C‑CGGtg > 1:866690/1‑149 (MQ=255) cttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGtttt < 2:809923/150‑1 (MQ=255) aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGGCCGCGCGAGAAATAATTACGGGAGGACTTTTTAGGAAAGGATATCTA‑A‑CGGTTTTGACg > 2:1070191/1‑150 (MQ=255) aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACg < 2:751682/150‑1 (MQ=255) aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACg < 1:1046672/150‑1 (MQ=255) cTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACgg < 2:967329/150‑1 (MQ=255) ttGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGc < 2:1559438/150‑1 (MQ=255) tGATGCCTCCCTGAACGTGTCCATTTGCCACATGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGca < 1:448687/150‑1 (MQ=255) gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGcac < 2:61181/150‑1 (MQ=255) gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGcac < 1:959051/150‑1 (MQ=255) gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGcac < 2:796622/150‑1 (MQ=255) tGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACAt < 1:207102/150‑1 (MQ=255) gCCTCCCTGCACGTGGCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTGACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACAtt < 1:284738/150‑1 (MQ=255) ccTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTc < 1:1269850/150‑1 (MQ=255) gtgtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGAcc < 2:1699465/150‑1 (MQ=255) gtgtCCATTTGCCACAAGTATAACCGGCAAATCCGGGTTACGCCGGGAGAGCAGCAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGTACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGAcc < 1:438624/150‑1 (MQ=255) aagtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGAcc < 2:1522198/148‑1 (MQ=255) gtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCaa < 1:815292/150‑1 (MQ=255) gtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCaa < 2:260250/150‑1 (MQ=255) ccATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCGCGCGCGAGAAATCATTACGGCAGGCCTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCTCATTATGGTACGGCCCAAcc > 2:1722351/1‑149 (MQ=255) ccATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCCCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACg > 2:979864/1‑150 (MQ=255) tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GTGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATg < 2:1592572/150‑1 (MQ=255) tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACTGACCAACGTCATg < 2:185085/150‑1 (MQ=255) tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATg < 2:1409729/150‑1 (MQ=255) gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGAATTCCCGCGCGAGAAATAAATAACGCCGGACATTAGGGGGAAAGGTAACAA‑C‑CCGGTTTGTCGGCCCAATAAGCTACGGCCCAAcatcatta > 2:650884/1‑148 (MQ=255) aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTg < 1:522995/150‑1 (MQ=255) aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTg < 1:165182/150‑1 (MQ=255) gCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGG‑GGGGAAGATTTGCCCCCCGCGCAAAAAATAAGGCAGGGCGTTTTAGGAAAGGATAAAAA‑CACGGTTTT‑TCGGGACAATAAGGTTAGGACCAAACTCAAGAGTCGGGACCTggg > 2:1494091/1‑150 (MQ=255) cGGCAAATCCGGGTTACGCCGGGAGCGCAGAAGCGCGGGGCAAAATTACCC‑CCCGCGAAATAATTTCGGGACGCCCTTATAGGTAAGGCTGACAACC‑CGGTTTTTAC‑GCACATTATGGTACCGAGCACCGTCCAGCGTGGGCACCCgggg > 2:719805/1‑149 (MQ=255) ggCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGGAATGATTAAGGCAGGACTTTAGCGGAAAGGATAACAA‑A‑CGGTTTTGACGGCCCATTCAGGTACGGACCAACGTCAAGAGTGCGGACCAGGGaa > 2:78455/1‑150 (MQ=255) ggCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCCCGCGCGAAATCATTACGGCAGGACATTAACGGAAAAGATAAAAA‑C‑CGGTTTTGACCGCACAATATGGGAACGACCAACGTCAAGACTCGGCACCAGGGaa > 2:901710/1‑150 (MQ=255) cAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGAGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAAt < 1:901710/150‑1 (MQ=255) tCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCTAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTa < 2:952307/150‑1 (MQ=255) tCCGGGGTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGAAAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTa < 2:328920/150‑1 (MQ=255) ccGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTAt < 2:1068758/150‑1 (MQ=255) cGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc < 1:215901/150‑1 (MQ=255) ggTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGTTTGCCCGCGGGAGCAAACATTTCCGCCGGTCTTTAGGGGGAAGGGTAACGA‑C‑CGGGTTTGCCGGGCCCGTCGGGTACGGAGCACCGTGATGAGTCGGCAGCTGGGGAATCttttggg > 2:118915/1‑145 (MQ=255) ggTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCgg < 1:678255/150‑1 (MQ=255) gTTACGCCGGGAGATCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGc < 2:162926/150‑1 (MQ=255) gTTACGCCGGGAGAGCAGAAGCGC‑GGGCCAGATTTGCCCCGCCGGAAAAAATTACAGCAGGACTTTATAGGTAAAGATAAAAA‑A‑ACGTTTTTACGGCACAATATTGTTACGAAAAACCTCATGAATCGGCACCAGGGAAAAATTAtattc > 2:618545/1‑146 (MQ=255) ttACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTTCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGGTTTGACGGCACATTCTGGTACGGCCCACCGTCATGAGTCGGCCCCTGGGAAATCTTATCGGCa > 1:1126639/1‑150 (MQ=255) tACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCaa < 2:826811/150‑1 (MQ=255) cGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACATTAGCGGAAAGGATAACTA‑C‑CGGGTTTGCCGGCACAATAAGGTAAGGACCCACGTCATGAGTCGGCACCAGGGAAATCTTATCGGCAACg > 2:1005530/1‑150 (MQ=255) ggAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCAt < 1:218090/150‑1 (MQ=255) ggAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAAACATTACGGCAGGACTTTAGCGGTAAGGATAACTA‑C‑CGGTTTTGACGGCACATTAAGGTACGGGCCCACGTCATTAGTCGGCACCAGGGAAATATTATCGGCCACGTccct > 2:199223/1‑148 (MQ=255) agagCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGc < 2:1396728/150‑1 (MQ=255) gagCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGcc < 2:1429700/150‑1 (MQ=255) gCAGAAGCGC‑GGGCAATATTGCCCGCGCGAGAAATCATTACGGCAGGTCATTAGCGGTAAGGATAACAA‑C‑CGGTTTTTTCGGCACAATATGGTACGGCCCAACCTAATTAGTCGGCACCAGGGAAATCATATAGGCAACGTCCCTGccat > 2:969097/1‑148 (MQ=255) gCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATACCTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCtt < 1:331006/150‑1 (MQ=255) gCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCtt < 1:960204/150‑1 (MQ=255) cAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACTGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCttt < 1:1461169/150‑1 (MQ=255) tctcgcgc‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc > 1:1201743/5‑150 (MQ=255) tctcgcgc‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc > 1:1208097/5‑150 (MQ=255) ctcgcgc‑GGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTGTGGTACGGACCAACGTCATGAGTCGGCACCTGGGTAATCTTATCGGCAACGTCCATGCCTTTca > 1:671610/4‑150 (MQ=255) gc‑gGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACt < 2:1401968/150‑1 (MQ=255) ggCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa > 2:876071/1‑150 (MQ=255) ggCAAGATTGCCCGCTCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa < 1:182271/150‑1 (MQ=255) ggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa > 1:652140/1‑150 (MQ=255) ggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa > 2:977008/1‑150 (MQ=255) cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc < 1:908268/150‑1 (MQ=255) cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc < 1:969097/150‑1 (MQ=255) aaGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCa < 1:1040226/150‑1 (MQ=255) aGATTGACCGCGCGAGAAATCATTAGGGCAGGTCTTTAGCTGAAAGCTTAACTA‑C‑CGGTTTTGACTGCACATCCTGGTACGGACCAACTTCATGAGGCGGCAACTCGGAAATCTTATCTGCAACGTCCATGCCTTTCACCACTTTACCaa < 1:102468/150‑1 (MQ=255) | CTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGC‑GGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTA‑C‑CGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA > NC_000913/3491555‑3491852 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |