Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,491,704 G→T 7.3% intergenic (‑84/+21) yhfG ← / ← ppiA putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,491,7040GT 7.3% 235.3 / 6.3 94intergenic (‑84/+21)yhfG/ppiAputative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
Reads supporting (aligned to +/- strand):  ref base G (24/61);  new base T (3/3);  total (30/64)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

CTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA  >  NC_000913/3491555‑3491852
                                                                                                                                                      |                                                                                                                                                      
cTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAg                                                                                                                                                        <  1:1139384/150‑1 (MQ=255)
    aaaTTAAGCTTAAGCGGAATCTCCCAGCTACGGGTCTTTTGCTTATCGGTGTTGTTCTTCAATTGATTCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTg                                                                                                                                                    <  2:1032276/150‑1 (MQ=255)
     aaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCACGGGCAAGTTTGc                                                                                                                                                   >  2:102927/1‑150 (MQ=255)
        tacgattacgCTGAAGCTCCCAGAGCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcg                                                                                                                                                <  1:1695495/150‑1 (MQ=255)
          cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcg                                                                                                                                              >  2:207102/1‑150 (MQ=255)
           tattacgCTGAAGGTCCCAGAGACGGGACTTTTGCTTATCGTGGAGTTTCTTCACTTGATGCGTCCCTGAACGTGTCCATTTGCCACTAGTATAATCGGCAAATCCGGGTTACGGCGGGAGAGCTGAAGCGCGGGCAATCTTGCCcgcgc                                                                                                                                             <  2:1327153/149‑1 (MQ=255)
            attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCTCCGGCAAAATTTGCcccgcg                                                                                                                                            >  2:494280/1‑150 (MQ=255)
            attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcgct                                                                                                                                            <  1:1683220/150‑2 (MQ=255)
            attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCAATTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcgct                                                                                                                                            <  1:843700/150‑2 (MQ=255)
             ttacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcgcgg                                                                                                                                           >  1:900221/1‑149 (MQ=255)
               acgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCgaga                                                                                                                                         <  1:494280/150‑1 (MQ=255)
                 gCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGCAGAATGGCCGCGCGAGtaa                                                                                                                                       >  1:1068758/1‑150 (MQ=255)
                      cgCTCCCAGAGCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAtt                                                                                                                                  <  1:1080323/149‑1 (MQ=255)
                      aGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAtt                                                                                                                                  <  1:92439/150‑1 (MQ=255)
                        cTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGGCAGATTTACCCCCCGAGaaaaaaaata                                                                                                                                >  1:1396728/1‑143 (MQ=255)
                          cccAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAACTACGGGTTACTCCGGGAGAGCAGAAGCGCGGGCCAGATTGCCCGCGCGAGCACTCATTACgg                                                                                                                              >  2:93229/1‑150 (MQ=255)
                               agaCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGACAAAACAATAAGGCAGGa                                                                                                                         >  2:215901/1‑150 (MQ=255)
                                 aCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACt                                                                                                                       <  2:338767/150‑1 (MQ=255)
                                   gggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACttt                                                                                                                     <  1:1314181/150‑1 (MQ=255)
                                     gACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAg                                                                                                                   <  1:1034508/150‑1 (MQ=255)
                                      aCTTTTCCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGGGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGc                                                                                                                  <  1:1376491/150‑1 (MQ=255)
                                        ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                                <  2:1222359/150‑1 (MQ=255)
                                        ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGGGTCAATTTGCCCCACGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                                <  2:1463533/150‑1 (MQ=255)
                                        ttttGCTTATCGGAGAGTTTCTTCACTTGATGCCTCCCTGACCGTGTCCATTTGCCACAATTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAGTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                                <  2:867734/150‑1 (MQ=255)
                                         tttGCTTCTCTGGGAGTTTCTTCACTTTATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCTGCAACTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGa                                                                                                               <  2:906441/150‑1 (MQ=255)
                                             cTTCTCGGGGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCCCAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAgg                                                                                                           <  2:1126867/150‑1 (MQ=255)
                                               tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGAt                                                                                                         <  1:431442/150‑1 (MQ=255)
                                                aTCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAGTCATTACGGCAGGACTTTAGCGGAAAGGATa                                                                                                        <  2:28792/150‑1 (MQ=255)
                                                 tCGGTGAGTTTCTTCACTTGATGCCTCCCTGACCGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATaa                                                                                                       <  1:543567/150‑1 (MQ=255)
                                                   ggTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCCCAAGTATAAGAGGCAAATCCGGGTTACGCCGGGTGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACt                                                                                                     <  1:333948/150‑1 (MQ=255)
                                                      gAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTAcc                                                                                                <  2:509595/150‑1 (MQ=255)
                                                        gTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGAACAATGGCCCCCCGAGAAAAAAATACCGCACGTCGTTATCGGATAGGATAACtcaccg                                                                                                >  2:707004/1‑145 (MQ=255)
                                                          ttcttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGCTTTGCCCCGCCGCAAAACAATACCGCAGGGCGTGTGAGGCTACGATAACTACCGGtg                                                                                            >  1:866690/1‑149 (MQ=255)
                                                            cttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGtttt                                                                                          <  2:809923/150‑1 (MQ=255)
                                                                aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGGCCGCGCGAGAAATAATTACGGGAGGACTTTTTAGGAAAGGATATCTAACGGTTTTGACg                                                                                      >  2:1070191/1‑150 (MQ=255)
                                                                aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACg                                                                                      <  2:751682/150‑1 (MQ=255)
                                                                aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACg                                                                                      <  1:1046672/150‑1 (MQ=255)
                                                                 cTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACgg                                                                                     <  2:967329/150‑1 (MQ=255)
                                                                  ttGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGc                                                                                    <  2:1559438/150‑1 (MQ=255)
                                                                   tGATGCCTCCCTGAACGTGTCCATTTGCCACATGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGca                                                                                   <  1:448687/150‑1 (MQ=255)
                                                                    gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGcac                                                                                  <  2:61181/150‑1 (MQ=255)
                                                                    gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGcac                                                                                  <  1:959051/150‑1 (MQ=255)
                                                                    gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGcac                                                                                  <  2:796622/150‑1 (MQ=255)
                                                                      tGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACAt                                                                                <  1:207102/150‑1 (MQ=255)
                                                                       gCCTCCCTGCACGTGGCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTGACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACAtt                                                                               <  1:284738/150‑1 (MQ=255)
                                                                        ccTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTc                                                                              <  1:1269850/150‑1 (MQ=255)
                                                                                   gtgtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAcc                                                                   <  2:1699465/150‑1 (MQ=255)
                                                                                   gtgtCCATTTGCCACAAGTATAACCGGCAAATCCGGGTTACGCCGGGAGAGCAGCAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGTACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAcc                                                                   <  1:438624/150‑1 (MQ=255)
                                                                                   aagtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAcc                                                                   <  2:1522198/148‑1 (MQ=255)
                                                                                     gtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCaa                                                                 <  1:815292/150‑1 (MQ=255)
                                                                                     gtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCaa                                                                 <  2:260250/150‑1 (MQ=255)
                                                                                       ccATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCGCGCGCGAGAAATCATTACGGCAGGCCTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCTCATTATGGTACGGCCCAAcc                                                               >  2:1722351/1‑149 (MQ=255)
                                                                                       ccATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCCCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACg                                                               >  2:979864/1‑150 (MQ=255)
                                                                                            tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGTGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATg                                                          <  2:1592572/150‑1 (MQ=255)
                                                                                            tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACTGACCAACGTCATg                                                          <  2:185085/150‑1 (MQ=255)
                                                                                            tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATg                                                          <  2:1409729/150‑1 (MQ=255)
                                                                                             gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAATTCCCGCGCGAGAAATAAATAACGCCGGACATTAGGGGGAAAGGTAACAACCCGGTTTGTCGGCCCAATAAGCTACGGCCCAAcatcatta                                                         >  2:650884/1‑148 (MQ=255)
                                                                                                        aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTg                                              <  1:522995/150‑1 (MQ=255)
                                                                                                        aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTg                                              <  1:165182/150‑1 (MQ=255)
                                                                                                          gCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGGAAGATTTGCCCCCCGCGCAAAAAATAAGGCAGGGCGTTTTAGGAAAGGATAAAAACACGGTTTTTCGGGACAATAAGGTTAGGACCAAACTCAAGAGTCGGGACCTggg                                            >  2:1494091/1‑150 (MQ=255)
                                                                                                           cGGCAAATCCGGGTTACGCCGGGAGCGCAGAAGCGCGGGGCAAAATTACCCCCCGCGAAATAATTTCGGGACGCCCTTATAGGTAAGGCTGACAACCCGGTTTTTACGCACATTATGGTACCGAGCACCGTCCAGCGTGGGCACCCgggg                                           >  2:719805/1‑149 (MQ=255)
                                                                                                            ggCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGGAATGATTAAGGCAGGACTTTAGCGGAAAGGATAACAAACGGTTTTGACGGCCCATTCAGGTACGGACCAACGTCAAGAGTGCGGACCAGGGaa                                          >  2:78455/1‑150 (MQ=255)
                                                                                                            ggCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCCCGCGCGAAATCATTACGGCAGGACATTAACGGAAAAGATAAAAACCGGTTTTGACCGCACAATATGGGAACGACCAACGTCAAGACTCGGCACCAGGGaa                                          >  2:901710/1‑150 (MQ=255)
                                                                                                              cAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGAGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAAt                                        <  1:901710/150‑1 (MQ=255)
                                                                                                                  tCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCTAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTa                                    <  2:952307/150‑1 (MQ=255)
                                                                                                                  tCCGGGGTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGAAAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTa                                    <  2:328920/150‑1 (MQ=255)
                                                                                                                   ccGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTAt                                   <  2:1068758/150‑1 (MQ=255)
                                                                                                                    cGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                  <  1:215901/150‑1 (MQ=255)
                                                                                                                      ggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGTTTGCCCGCGGGAGCAAACATTTCCGCCGGTCTTTAGGGGGAAGGGTAACGACCGGGTTTGCCGGGCCCGTCGGGTACGGAGCACCGTGATGAGTCGGCAGCTGGGGAATCttttggg                                >  2:118915/1‑145 (MQ=255)
                                                                                                                      ggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCgg                                <  1:678255/150‑1 (MQ=255)
                                                                                                                       gTTACGCCGGGAGATCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGc                               <  2:162926/150‑1 (MQ=255)
                                                                                                                       gTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTTGCCCCGCCGGAAAAAATTACAGCAGGACTTTATAGGTAAAGATAAAAAAACGTTTTTACGGCACAATATTGTTACGAAAAACCTCATGAATCGGCACCAGGGAAAAATTAtattc                               >  2:618545/1‑146 (MQ=255)
                                                                                                                        ttACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGGTTTGACGGCACATTCTGGTACGGCCCACCGTCATGAGTCGGCCCCTGGGAAATCTTATCGGCa                              >  1:1126639/1‑150 (MQ=255)
                                                                                                                         tACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCaa                             <  2:826811/150‑1 (MQ=255)
                                                                                                                           cGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACATTAGCGGAAAGGATAACTACCGGGTTTGCCGGCACAATAAGGTAAGGACCCACGTCATGAGTCGGCACCAGGGAAATCTTATCGGCAACg                           >  2:1005530/1‑150 (MQ=255)
                                                                                                                                ggAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCAt                      <  1:218090/150‑1 (MQ=255)
                                                                                                                                ggAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAAACATTACGGCAGGACTTTAGCGGTAAGGATAACTACCGGTTTTGACGGCACATTAAGGTACGGGCCCACGTCATTAGTCGGCACCAGGGAAATATTATCGGCCACGTccct                      >  2:199223/1‑148 (MQ=255)
                                                                                                                                  agagCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGc                    <  2:1396728/150‑1 (MQ=255)
                                                                                                                                   gagCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGcc                   <  2:1429700/150‑1 (MQ=255)
                                                                                                                                     gCAGAAGCGCGGGCAATATTGCCCGCGCGAGAAATCATTACGGCAGGTCATTAGCGGTAAGGATAACAACCGGTTTTTTCGGCACAATATGGTACGGCCCAACCTAATTAGTCGGCACCAGGGAAATCATATAGGCAACGTCCCTGccat                 >  2:969097/1‑148 (MQ=255)
                                                                                                                                     gCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATACCTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCtt                 <  1:331006/150‑1 (MQ=255)
                                                                                                                                     gCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCtt                 <  1:960204/150‑1 (MQ=255)
                                                                                                                                      cAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACTGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCttt                <  1:1461169/150‑1 (MQ=255)
                                                                                                                                       tctcgcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc               >  1:1201743/5‑150 (MQ=255)
                                                                                                                                       tctcgcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc               >  1:1208097/5‑150 (MQ=255)
                                                                                                                                        ctcgcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTGTGGTACGGACCAACGTCATGAGTCGGCACCTGGGTAATCTTATCGGCAACGTCCATGCCTTTca              >  1:671610/4‑150 (MQ=255)
                                                                                                                                             gcgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACt         <  2:1401968/150‑1 (MQ=255)
                                                                                                                                                 ggCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa      >  2:876071/1‑150 (MQ=255)
                                                                                                                                                 ggCAAGATTGCCCGCTCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa      <  1:182271/150‑1 (MQ=255)
                                                                                                                                                 ggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa      >  1:652140/1‑150 (MQ=255)
                                                                                                                                                 ggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa      >  2:977008/1‑150 (MQ=255)
                                                                                                                                                   cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc    <  1:908268/150‑1 (MQ=255)
                                                                                                                                                   cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc    <  1:969097/150‑1 (MQ=255)
                                                                                                                                                    aaGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCa   <  1:1040226/150‑1 (MQ=255)
                                                                                                                                                     aGATTGACCGCGCGAGAAATCATTAGGGCAGGTCTTTAGCTGAAAGCTTAACTACCGGTTTTGACTGCACATCCTGGTACGGACCAACTTCATGAGGCGGCAACTCGGAAATCTTATCTGCAACGTCCATGCCTTTCACCACTTTACCaa  <  1:102468/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CTGAAAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAA  >  NC_000913/3491555‑3491852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: