Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,491,705 A→C 6.4% intergenic (‑85/+20) yhfG ← / ← ppiA putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,491,7050AC 6.4% 252.0 / 4.3 94intergenic (‑85/+20)yhfG/ppiAputative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
Reads supporting (aligned to +/- strand):  ref base A (25/60);  new base C (3/4);  total (30/64)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAAT  >  NC_000913/3491559‑3491854
                                                                                                                                                   |                                                                                                                                                       
aaaTTAAGCTTAAGCGGAATCTCCCAGCTACGGGTCTTTTGCTTATCGGTGTTGTTCTTCAATTGATTCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTg                                                                                                                                                      <  2:1032276/150‑1 (MQ=255)
 aaTTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCACGGGCAAGTTTGc                                                                                                                                                     >  2:102927/1‑150 (MQ=255)
    tacgattacgCTGAAGCTCCCAGAGCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcg                                                                                                                                                  <  1:1695495/150‑1 (MQ=255)
      cgattacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcg                                                                                                                                                >  2:207102/1‑150 (MQ=255)
       tattacgCTGAAGGTCCCAGAGACGGGACTTTTGCTTATCGTGGAGTTTCTTCACTTGATGCGTCCCTGAACGTGTCCATTTGCCACTAGTATAATCGGCAAATCCGGGTTACGGCGGGAGAGCTGAAGCGCGGGCAATCTTGCCcgcgc                                                                                                                                               <  2:1327153/149‑1 (MQ=255)
        attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCTCCGGCAAAATTTGCcccgcg                                                                                                                                              >  2:494280/1‑150 (MQ=255)
        attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcgct                                                                                                                                              <  1:1683220/150‑2 (MQ=255)
        attacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCAATTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAATCTTGCCcgcgct                                                                                                                                              <  1:843700/150‑2 (MQ=255)
         ttacgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCcgcgcgg                                                                                                                                             >  1:900221/1‑149 (MQ=255)
           acgCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCgaga                                                                                                                                           <  1:494280/150‑1 (MQ=255)
             gCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGCAGAATGGCCGCGCGAGtaa                                                                                                                                         >  1:1068758/1‑150 (MQ=255)
                  cgCTCCCAGAGCCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAtt                                                                                                                                    <  1:1080323/149‑1 (MQ=255)
                  aGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCAtt                                                                                                                                    <  1:92439/150‑1 (MQ=255)
                    cTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGGCAGATTTACCCCCCGAGaaaaaaaata                                                                                                                                  >  1:1396728/1‑143 (MQ=255)
                      cccAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAACTACGGGTTACTCCGGGAGAGCAGAAGCGCGGGCCAGATTGCCCGCGCGAGCACTCATTACgg                                                                                                                                >  2:93229/1‑150 (MQ=255)
                           agaCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGACAAAACAATAAGGCAGGa                                                                                                                           >  2:215901/1‑150 (MQ=255)
                             aCGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACt                                                                                                                         <  2:338767/150‑1 (MQ=255)
                               gggACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACttt                                                                                                                       <  1:1314181/150‑1 (MQ=255)
                                 gACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAg                                                                                                                     <  1:1034508/150‑1 (MQ=255)
                                  aCTTTTCCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGGGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGc                                                                                                                    <  1:1376491/150‑1 (MQ=255)
                                    ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                                  <  2:1222359/150‑1 (MQ=255)
                                    ttttGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGGGTCAATTTGCCCCACGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                                  <  2:1463533/150‑1 (MQ=255)
                                    ttttGCTTATCGGAGAGTTTCTTCACTTGATGCCTCCCTGACCGTGTCCATTTGCCACAATTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAGTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCgg                                                                                                                  <  2:867734/150‑1 (MQ=255)
                                     tttGCTTCTCTGGGAGTTTCTTCACTTTATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCTGCAACTCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGa                                                                                                                 <  2:906441/150‑1 (MQ=255)
                                         cTTCTCGGGGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCCCAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAgg                                                                                                             <  2:1126867/150‑1 (MQ=255)
                                           tATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGAt                                                                                                           <  1:431442/150‑1 (MQ=255)
                                            aTCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAGTCATTACGGCAGGACTTTAGCGGAAAGGATa                                                                                                          <  2:28792/150‑1 (MQ=255)
                                             tCGGTGAGTTTCTTCACTTGATGCCTCCCTGACCGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATaa                                                                                                         <  1:543567/150‑1 (MQ=255)
                                               ggTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCCCAAGTATAAGAGGCAAATCCGGGTTACGCCGGGTGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACt                                                                                                       <  1:333948/150‑1 (MQ=255)
                                                  gAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTAcc                                                                                                  <  2:509595/150‑1 (MQ=255)
                                                    gTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGGAACAATGGCCCCCCGAGAAAAAAATACCGCACGTCGTTATCGGATAGGATAACtcaccg                                                                                                  >  2:707004/1‑145 (MQ=255)
                                                      ttcttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCCAGCTTTGCCCCGCCGCAAAACAATACCGCAGGGCGTGTGAGGCTACGATAACTACCGGtg                                                                                              >  1:866690/1‑149 (MQ=255)
                                                        cttcACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGtttt                                                                                            <  2:809923/150‑1 (MQ=255)
                                                            aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGGCCGCGCGAGAAATAATTACGGGAGGACTTTTTAGGAAAGGATATCTAACGGTTTTGACg                                                                                        >  2:1070191/1‑150 (MQ=255)
                                                            aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACg                                                                                        <  2:751682/150‑1 (MQ=255)
                                                            aCTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACg                                                                                        <  1:1046672/150‑1 (MQ=255)
                                                             cTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACgg                                                                                       <  2:967329/150‑1 (MQ=255)
                                                              ttGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGc                                                                                      <  2:1559438/150‑1 (MQ=255)
                                                               tGATGCCTCCCTGAACGTGTCCATTTGCCACATGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGca                                                                                     <  1:448687/150‑1 (MQ=255)
                                                                gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGcac                                                                                    <  2:796622/150‑1 (MQ=255)
                                                                gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGcac                                                                                    <  2:61181/150‑1 (MQ=255)
                                                                gATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGcac                                                                                    <  1:959051/150‑1 (MQ=255)
                                                                  tGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACAt                                                                                  <  1:207102/150‑1 (MQ=255)
                                                                   gCCTCCCTGCACGTGGCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTGACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACAtt                                                                                 <  1:284738/150‑1 (MQ=255)
                                                                    ccTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTc                                                                                <  1:1269850/150‑1 (MQ=255)
                                                                               gtgtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAcc                                                                     <  2:1699465/150‑1 (MQ=255)
                                                                               gtgtCCATTTGCCACAAGTATAACCGGCAAATCCGGGTTACGCCGGGAGAGCAGCAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGTACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAcc                                                                     <  1:438624/150‑1 (MQ=255)
                                                                               aagtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGAcc                                                                     <  2:1522198/148‑1 (MQ=255)
                                                                                 gtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCaa                                                                   <  2:260250/150‑1 (MQ=255)
                                                                                 gtCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCaa                                                                   <  1:815292/150‑1 (MQ=255)
                                                                                   ccATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCGCGCGCGAGAAATCATTACGGCAGGCCTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCTCATTATGGTACGGCCCAAcc                                                                 >  2:1722351/1‑149 (MQ=255)
                                                                                   ccATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCCCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACg                                                                 >  2:979864/1‑150 (MQ=255)
                                                                                        tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGTGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATg                                                            <  2:1592572/150‑1 (MQ=255)
                                                                                        tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACTGACCAACGTCATg                                                            <  2:185085/150‑1 (MQ=255)
                                                                                        tGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATg                                                            <  2:1409729/150‑1 (MQ=255)
                                                                                         gCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGAATTCCCGCGCGAGAAATAAATAACGCCGGACATTAGGGGGAAAGGTAACAACCCGGTTTGTCGGCCCAATAAGCTACGGCCCAAcatcatta                                                           >  2:650884/1‑148 (MQ=255)
                                                                                                    aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTg                                                <  1:522995/150‑1 (MQ=255)
                                                                                                    aaGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTg                                                <  1:165182/150‑1 (MQ=255)
                                                                                                      gCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGGGGGGAAGATTTGCCCCCCGCGCAAAAAATAAGGCAGGGCGTTTTAGGAAAGGATAAAAACACGGTTTTTCGGGACAATAAGGTTAGGACCAAACTCAAGAGTCGGGACCTggg                                              >  2:1494091/1‑150 (MQ=255)
                                                                                                       cGGCAAATCCGGGTTACGCCGGGAGCGCAGAAGCGCGGGGCAAAATTACCCCCCGCGAAATAATTTCGGGACGCCCTTATAGGTAAGGCTGACAACCCGGTTTTTACGCACATTATGGTACCGAGCACCGTCCAGCGTGGGCACCCgggg                                             >  2:719805/1‑149 (MQ=255)
                                                                                                        ggCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGGAATGATTAAGGCAGGACTTTAGCGGAAAGGATAACAAACGGTTTTGACGGCCCATTCAGGTACGGACCAACGTCAAGAGTGCGGACCAGGGaa                                            >  2:78455/1‑150 (MQ=255)
                                                                                                        ggCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCCCGCGCGAAATCATTACGGCAGGACATTAACGGAAAAGATAAAAACCGGTTTTGACCGCACAATATGGGAACGACCAACGTCAAGACTCGGCACCAGGGaa                                            >  2:901710/1‑150 (MQ=255)
                                                                                                          cAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGAGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAAt                                          <  1:901710/150‑1 (MQ=255)
                                                                                                              tCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCTAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTa                                      <  2:952307/150‑1 (MQ=255)
                                                                                                              tCCGGGGTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGAAAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTa                                      <  2:328920/150‑1 (MQ=255)
                                                                                                               ccGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTAt                                     <  2:1068758/150‑1 (MQ=255)
                                                                                                                cGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATc                                    <  1:215901/150‑1 (MQ=255)
                                                                                                                  ggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGTTTGCCCGCGGGAGCAAACATTTCCGCCGGTCTTTAGGGGGAAGGGTAACGACCGGGTTTGCCGGGCCCGTCGGGTACGGAGCACCGTGATGAGTCGGCAGCTGGGGAATCttttggg                                  >  2:118915/1‑145 (MQ=255)
                                                                                                                  ggTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCgg                                  <  1:678255/150‑1 (MQ=255)
                                                                                                                   gTTACGCCGGGAGATCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGc                                 <  2:162926/150‑1 (MQ=255)
                                                                                                                   gTTACGCCGGGAGAGCAGAAGCGCGGGCCAGATTTGCCCCGCCGGAAAAAATTACAGCAGGACTTTATAGGTAAAGATAAAAAAACGTTTTTACGGCACAATATTGTTACGAAAAACCTCATGAATCGGCACCAGGGAAAAATTAtattc                                 >  2:618545/1‑146 (MQ=255)
                                                                                                                    ttACGCCGGGAGAGCAGAAGCGCGGGCAAGATTTCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGGTTTGACGGCACATTCTGGTACGGCCCACCGTCATGAGTCGGCCCCTGGGAAATCTTATCGGCa                                >  1:1126639/1‑150 (MQ=255)
                                                                                                                     tACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCaa                               <  2:826811/150‑1 (MQ=255)
                                                                                                                       cGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACATTAGCGGAAAGGATAACTACCGGGTTTGCCGGCACAATAAGGTAAGGACCCACGTCATGAGTCGGCACCAGGGAAATCTTATCGGCAACg                             >  2:1005530/1‑150 (MQ=255)
                                                                                                                            ggAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCAt                        <  1:218090/150‑1 (MQ=255)
                                                                                                                            ggAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAAACATTACGGCAGGACTTTAGCGGTAAGGATAACTACCGGTTTTGACGGCACATTAAGGTACGGGCCCACGTCATTAGTCGGCACCAGGGAAATATTATCGGCCACGTccct                        >  2:199223/1‑148 (MQ=255)
                                                                                                                              agagCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGc                      <  2:1396728/150‑1 (MQ=255)
                                                                                                                               gagCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGcc                     <  2:1429700/150‑1 (MQ=255)
                                                                                                                                 gCAGAAGCGCGGGCAATATTGCCCGCGCGAGAAATCATTACGGCAGGTCATTAGCGGTAAGGATAACAACCGGTTTTTTCGGCACAATATGGTACGGCCCAACCTAATTAGTCGGCACCAGGGAAATCATATAGGCAACGTCCCTGccat                   >  2:969097/1‑148 (MQ=255)
                                                                                                                                 gCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATACCTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCtt                   <  1:331006/150‑1 (MQ=255)
                                                                                                                                 gCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCtt                   <  1:960204/150‑1 (MQ=255)
                                                                                                                                  cAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACTGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCttt                  <  1:1461169/150‑1 (MQ=255)
                                                                                                                                   tctcgcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc                 >  1:1208097/5‑150 (MQ=255)
                                                                                                                                   tctcgcgcGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTc                 >  1:1201743/5‑150 (MQ=255)
                                                                                                                                    ctcgcgcGGGCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTGTGGTACGGACCAACGTCATGAGTCGGCACCTGGGTAATCTTATCGGCAACGTCCATGCCTTTca                >  1:671610/4‑150 (MQ=255)
                                                                                                                                         gcgGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACt           <  2:1401968/150‑1 (MQ=255)
                                                                                                                                             ggCAATCTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa        >  2:876071/1‑150 (MQ=255)
                                                                                                                                             ggCAAGATTGCCCGCTCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa        <  1:182271/150‑1 (MQ=255)
                                                                                                                                             ggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa        >  2:977008/1‑150 (MQ=255)
                                                                                                                                             ggCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTa        >  1:652140/1‑150 (MQ=255)
                                                                                                                                               cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc      <  1:969097/150‑1 (MQ=255)
                                                                                                                                               cAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTAcc      <  1:908268/150‑1 (MQ=255)
                                                                                                                                                aaGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCa     <  1:1040226/150‑1 (MQ=255)
                                                                                                                                                 aGATTGACCGCGCGAGAAATCATTAGGGCAGGTCTTTAGCTGAAAGCTTAACTACCGGTTTTGACTGCACATCCTGGTACGGACCAACTTCATGAGGCGGCAACTCGGAAATCTTATCTGCAACGTCCATGCCTTTCACCACTTTACCaa    <  1:102468/150‑1 (MQ=255)
                                                                                                                                                   aTTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAAt  >  1:331558/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                       
AAATTACGATTACGCTGAAGCTCCCAGAGACGGGACTTTTGCTTATCGGTGAGTTTCTTCACTTGATGCCTCCCTGAACGTGTCCATTTGCCACAAGTATAAGCGGCAAATCCGGGTTACGCCGGGAGAGCAGAAGCGCGGGCAAGATTGCCCGCGCGAGAAATCATTACGGCAGGACTTTAGCGGAAAGGATAACTACCGGTTTTGACGGCACATTCTGGTACGGACCAACGTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAAT  >  NC_000913/3491559‑3491854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: