Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,512,306 G→C 5.1% I13M (ATC→ATG yhfZ ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,512,3060GC 5.1% 514.7 / 14.1 157I13M (ATC→ATGyhfZputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (79/69);  new base C (4/4);  total (83/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

Only 100 of 169 total aligned reads displayed.

CGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAA  >  NC_000913/3512157‑3512455
                                                                                                                                                     |                                                                                                                                                     
cGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGtg                                                                                                                                                       >  2:1603931/1‑150 (MQ=255)
  ttCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGtgat                                                                                                                                                     <  2:607199/150‑1 (MQ=255)
   tCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGtgatg                                                                                                                                                    >  2:578120/1‑150 (MQ=255)
      aTCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATgacg                                                                                                                                                 >  1:58349/1‑150 (MQ=255)
        ccGTATCGCTCCGATTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGGCGTGATgacgac                                                                                                                                               <  1:1308823/150‑1 (MQ=255)
          gTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGc                                                                                                                                             <  1:71576/150‑1 (MQ=255)
          gTATCGCTCCGCTTGATTCCAGCGGTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTTCCAGCTCATCTATGGGTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGGGATGACGACGc                                                                                                                                             <  1:1416611/150‑1 (MQ=255)
            aTCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCct                                                                                                                                           <  1:1281143/150‑1 (MQ=255)
              cGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTCATCACGACGctggc                                                                                                                                         <  2:540796/150‑5 (MQ=255)
              cGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTAACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCctta                                                                                                                                         >  1:1622463/1‑149 (MQ=255)
               gCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTCATCACGACGctggcc                                                                                                                                        <  2:616161/150‑6 (MQ=255)
               gCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTCATCACGACGctggcc                                                                                                                                        <  2:453251/150‑6 (MQ=255)
               gCTCCGCTTGAGTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTCATCACGACGctggcc                                                                                                                                        <  2:446810/150‑6 (MQ=255)
                    gCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCtttttt                                                                                                                                   <  1:819754/150‑1 (MQ=255)
                    gCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCtttttt                                                                                                                                   <  1:1459274/150‑1 (MQ=255)
                     cTTGATTCCAGCGTTTTCAACGGGGCCTGCGGCAGGCCATCGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTg                                                                                                                                  <  2:1482803/150‑1 (MQ=255)
                     cTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTg                                                                                                                                  <  2:802626/150‑1 (MQ=255)
                     cTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACACAACAAATAACGGGCCAGCGTCGTGATGCCGACGCCTTCTTTTTTg                                                                                                                                  >  1:1512247/1‑150 (MQ=255)
                       tGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGAt                                                                                                                                <  2:1456620/150‑1 (MQ=255)
                        gATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGACGTGATGACGACGCCTTCTTTTTTGATa                                                                                                                               >  1:317513/1‑150 (MQ=255)
                            ccAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACg                                                                                                                           >  2:1585952/1‑150 (MQ=255)
                            ccAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCGTCTTTTTTGATAAACg                                                                                                                           >  2:1156985/1‑150 (MQ=255)
                              aGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACACAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGtt                                                                                                                         >  2:1164459/1‑150 (MQ=255)
                                cGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCg                                                                                                                       <  1:984771/150‑1 (MQ=255)
                                      cAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCat                                                                                                                 >  1:547116/1‑150 (MQ=255)
                                      cAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCat                                                                                                                 >  2:1508740/1‑150 (MQ=255)
                                             gCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCCCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTATTTTTTGATAAACGTTCGACGCATAATAAAt                                                                                                          >  1:1227424/1‑150 (MQ=255)
                                              ccTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCCGCGTCGTGATGCCGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAAtt                                                                                                         >  2:1495963/1‑150 (MQ=255)
                                              ccTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCAATTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAAtt                                                                                                         <  2:1515514/150‑1 (MQ=255)
                                               cTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCACCAAATAAAGGCCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGAAGCATAATAAAttt                                                                                                        >  1:1088972/1‑150 (MQ=255)
                                                tGCGGCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAAtttt                                                                                                       <  1:1621357/150‑1 (MQ=255)
                                                  cGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCa                                                                                                     >  1:1247911/1‑150 (MQ=255)
                                                  cGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCa                                                                                                     <  2:20579/150‑1 (MQ=255)
                                                     cAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTa                                                                                                  <  1:1123862/150‑1 (MQ=255)
                                                     cAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGAAGCATAATAAATTTTCAGTa                                                                                                  >  2:240503/1‑150 (MQ=255)
                                                         ccAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAtt                                                                                              >  2:1246463/1‑150 (MQ=255)
                                                         ccAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAAtt                                                                                              >  2:1625462/1‑150 (MQ=255)
                                                             cGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAat                                                                                          <  1:572337/150‑1 (MQ=255)
                                                                 tGAACGGCATTCATTTGCCAGATCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCTTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATAttt                                                                                      <  1:19705/150‑1 (MQ=255)
                                                                  gAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCTACGCATAATAAATTTTCAGTAAATTGAATttttt                                                                                     >  2:860301/1‑149 (MQ=255)
                                                                   aaCGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTtat                                                                                    <  2:1190798/150‑1 (MQ=255)
                                                                       gCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTatatat                                                                                >  2:1715488/1‑148 (MQ=255)
                                                                        cattcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCtt                                                                               >  2:1234497/1‑150 (MQ=255)
                                                                         attcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTc                                                                              <  2:588234/150‑1 (MQ=255)
                                                                          ttcattTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCa                                                                             <  2:1297067/150‑1 (MQ=255)
                                                                          ttcattTGCCAGCTCATCTATGGTTTTCAATCGATAACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCa                                                                             <  2:1588743/150‑1 (MQ=255)
                                                                                  ccAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTg                                                                     <  2:1090232/150‑1 (MQ=255)
                                                                                   cAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGa                                                                    <  2:1247911/150‑1 (MQ=255)
                                                                                     gCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAg                                                                  <  1:763395/150‑1 (MQ=255)
                                                                                     gCTCATCTATGGTTTTCAATCGATAACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTTATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAg                                                                  <  2:847567/150‑1 (MQ=255)
                                                                                           cTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAgg                                                            >  2:883310/1‑150 (MQ=255)
                                                                                           cTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAgg                                                            >  2:494690/1‑150 (MQ=255)
                                                                                            tATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGgc                                                           >  1:1223620/1‑150 (MQ=255)
                                                                                             aTGGTTTTCAATCGTTTTCCGCACTTTTCACCCTACAAATATCGGGACAGCGTCGTGATGACGTCGCCTTCTTTTTTGATAAACGTTCGACGCATAATATATTTTCTGTAAATTGTATATTTATATCTTCAGGAATTTGAAGATAAGgcg                                                          <  1:851025/150‑1 (MQ=255)
                                                                                                  tttCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACa                                                     >  1:531069/1‑150 (MQ=255)
                                                                                                     cAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCg                                                  >  1:423562/1‑150 (MQ=255)
                                                                                                     cAATCGATAACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTATTATGATAAACGATCGACGCATAATAAATTTTCAGTAAATTTAATATTTATATCTTCATTAATTTGAAGATAAGGCTCAACAGCg                                                  >  2:285751/1‑150 (MQ=255)
                                                                                                      aaTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACTACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCATGAATTTGAAGATAAGGCGCAACAGCga                                                 >  2:1443996/1‑150 (MQ=255)
                                                                                                      aaTCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCga                                                 >  1:1039969/1‑150 (MQ=255)
                                                                                                         cGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAt                                              <  1:1126852/150‑1 (MQ=255)
                                                                                                          gATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAtg                                             <  2:1382913/150‑1 (MQ=255)
                                                                                                           aTTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAtgt                                            >  2:580557/1‑150 (MQ=255)
                                                                                                            ttACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAtgtg                                           <  1:1246463/150‑1 (MQ=255)
                                                                                                            ttACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAtgtg                                           <  2:1127567/150‑1 (MQ=255)
                                                                                                            ttACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGAtgtg                                           <  1:388312/150‑1 (MQ=255)
                                                                                                             tACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTgg                                          <  2:326395/150‑1 (MQ=255)
                                                                                                              aCCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGa                                         >  1:1372380/1‑150 (MQ=255)
                                                                                                              aCCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGa                                         >  2:732938/1‑150 (MQ=255)
                                                                                                               ccGCACTTTTCACCCCACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGCCGCATAATAAAGTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGaa                                        >  2:81407/1‑150 (MQ=255)
                                                                                                               ccGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGaa                                        >  1:1456313/1‑150 (MQ=255)
                                                                                                               ccGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTATTTGATAAACGTTCGACGCATAATAAATTATAAGTAAATTGATTATTTTTATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGaa                                        >  2:291886/1‑150 (MQ=255)
                                                                                                               ccGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTATTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGaa                                        >  2:855882/1‑150 (MQ=255)
                                                                                                                 gCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGCCGCCTTCTTTTTTGATAAACGTTCGATGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAAc                                      >  2:265378/1‑150 (MQ=255)
                                                                                                                  cACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACg                                     <  1:794751/150‑1 (MQ=255)
                                                                                                                  cACTTTTCACCCAACAAATAAAGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACg                                     <  2:1286397/150‑1 (MQ=255)
                                                                                                                    cTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAAc                                      <  1:1018308/147‑1 (MQ=255)
                                                                                                                      tttCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGa                                 >  1:391474/1‑150 (MQ=255)
                                                                                                                        tCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAgg                               >  2:1418686/1‑150 (MQ=255)
                                                                                                                         cACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGc                              <  2:423562/150‑1 (MQ=255)
                                                                                                                          aCCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGcc                             >  2:1298508/1‑149 (MQ=255)
                                                                                                                             caacaaATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACtt                          >  2:365052/1‑150 (MQ=255)
                                                                                                                             caacaaATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACtt                          >  2:134808/1‑150 (MQ=255)
                                                                                                                                   aTAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACAtt                    >  1:1296425/1‑150 (MQ=255)
                                                                                                                                     aaCGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACAtttt                  >  2:1321839/1‑150 (MQ=255)
                                                                                                                                       cGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACAtttttt                >  1:359997/1‑150 (MQ=255)
                                                                                                                                        gggCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTcaca                      <  1:1546502/143‑1 (MQ=255)
                                                                                                                                        gggCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTc               <  2:972003/150‑1 (MQ=255)
                                                                                                                                        aagaaggCGTCGTCATCACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTc               >  1:63235/7‑150 (MQ=255)
                                                                                                                                         ggCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCg              >  1:154187/1‑150 (MQ=255)
                                                                                                                                         ggCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCg              <  2:351779/150‑1 (MQ=255)
                                                                                                                                         agaaggCGTCGTCATCACGACGCTTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCg              >  1:216905/6‑150 (MQ=255)
                                                                                                                                         agaaggCGTCGTCATCACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCg              >  1:1133573/6‑150 (MQ=255)
                                                                                                                                          gCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCgg             <  2:1055036/150‑1 (MQ=255)
                                                                                                                                            aggCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGat           >  2:1626557/3‑150 (MQ=255)
                                                                                                                                             aGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGatt          <  2:1404594/150‑1 (MQ=255)
                                                                                                                                                gtcgtGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCATTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGATATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGattatt       <  1:1596584/150‑1 (MQ=255)
                                                                                                                                                 tcgtGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTg      <  2:360355/150‑1 (MQ=255)
                                                                                                                                                 tcgtGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTg      <  1:1234497/150‑1 (MQ=255)
                                                                                                                                                   gtGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCa    >  1:971939/1‑150 (MQ=255)
                                                                                                                                                     gatgaCGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCaaa  >  1:1713761/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACCGCACTTTTCACCCAACAAATAACGGGCCAGCGTCGTGATGACGACGCCTTCTTTTTTGATAAACGTTCGACGCATAATAAATTTTCAGTAAATTGAATATTTATATCTTCAGGAATTTGAAGATAAGGCGCAACAGCGAGATGTGGAAACGGCGAGGCACTTCACATTTTTTCGGATTATTGCAAA  >  NC_000913/3512157‑3512455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: