Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 431,109 A→T 8.0% intergenic (+157/+20) yajD → / ← tsx HNH nuclease family protein/nucleoside channel, receptor of phage T6 and colicin K

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913431,1090AT 8.0% 381.7 / 18.0 138intergenic (+157/+20)yajD/tsxHNH nuclease family protein/nucleoside channel, receptor of phage T6 and colicin K
Reads supporting (aligned to +/- strand):  ref base A (58/69);  new base T (6/5);  total (64/74)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

Only 100 of 139 total aligned reads displayed.

AAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACA  >  NC_000913/430963‑431258
                                                                                                                                                  |                                                                                                                                                     
aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCGATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAAt                                                                                                                                                    >  1:577883/1‑150 (MQ=255)
   ttGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATccc                                                                                                                                                 <  2:875261/150‑1 (MQ=255)
    tGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCg                                                                                                                                                <  1:471285/150‑1 (MQ=255)
    tGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCg                                                                                                                                                <  1:150123/150‑1 (MQ=255)
     gCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATTCCGGGAATAAATCCCgc                                                                                                                                               >  2:658067/1‑149 (MQ=255)
       cTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCa                                                                                                                                             >  2:375989/1‑150 (MQ=255)
       cTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCa                                                                                                                                             <  1:1200080/150‑1 (MQ=255)
       cTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATAAATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTAAACAATCAGAAATGCCGGGATTTATTCCCGGCa                                                                                                                                             <  1:1545465/150‑1 (MQ=25)
        tGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCTCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCACGGCAt                                                                                                                                            >  2:159277/1‑150 (MQ=255)
        tGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAt                                                                                                                                            <  1:1467611/150‑1 (MQ=255)
        tGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCAt                                                                                                                                            <  2:797503/150‑1 (MQ=255)
         gATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtt                                                                                                                                           <  2:1116934/150‑1 (MQ=255)
         gATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtt                                                                                                                                           <  2:1221434/150‑1 (MQ=255)
         gATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCAtt                                                                                                                                           >  2:961252/1‑150 (MQ=255)
           tGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAGGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCAtttc                                                                                                                                         <  1:1314289/150‑2 (MQ=255)
           tGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCAtttt                                                                                                                                         <  2:222624/150‑1 (MQ=255)
             cgcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtttctg                                                                                                                                       <  1:1619915/150‑4 (MQ=255)
             cgcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCa                                                                                                                                       >  1:441545/1‑150 (MQ=255)
             cgcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCa                                                                                                                                       >  2:608242/1‑150 (MQ=255)
                  cGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATc                                                                                                                                  >  2:1255634/1‑150 (MQ=255)
                    cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCTGACAAAGCGTTTACGCCGCATACGGCATGAACAAATCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCACGGCATTATCATAATCAg                                                                                                                                >  1:805075/1‑150 (MQ=255)
                    cTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAg                                                                                                                                >  1:1682651/1‑150 (MQ=255)
                      tATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGaa                                                                                                                              >  1:189199/1‑150 (MQ=255)
                         cAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCTCACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGtt                                                                                                                           >  1:934007/1‑150 (MQ=255)
                          aGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACATAGCGTTTACGCCGCATCCGGCATTATCAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAACTCCCGGCATTTTCATAATCAGAAGTTg                                                                                                                          <  1:1222840/150‑1 (MQ=255)
                          aGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTg                                                                                                                          >  2:1495355/1‑150 (MQ=255)
                          aGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTg                                                                                                                          >  2:8284/1‑150 (MQ=255)
                           ggCCTACGTAATTTCAGCAATATATTGAATTTACAAGCTTTTGTAGGCCGGACATAGCGTTTACGCCGCATCCGGCATGAACAAACCAAACGTTGTTAACAATCAGAAATGCAGGGAATAATTCCGGGCAATTTCAAAATCAGAATTTGt                                                                                                                         >  1:221562/1‑150 (MQ=25)
                               tACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAAcc                                                                                                                     <  2:707379/150‑1 (MQ=255)
                                 cGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACcta                                                                                                                   <  2:1337533/150‑1 (MQ=255)
                                  gTTATTTAAGCAATATATTGAATTTACGGGCTTTTGTTTGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACctac                                                                                                                  <  2:1005250/150‑1 (MQ=255)
                                    tATTTCAGCATTATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTTCGCCGCATCCGGAATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGCAGTCGTAACctacta                                                                                                                >  1:254951/1‑150 (MQ=255)
                                     aTTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCtactac                                                                                                               >  1:1511504/1‑150 (MQ=255)
                                       ttCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCa                                                                                                             <  2:445120/150‑1 (MQ=255)
                                        tCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAg                                                                                                            <  1:1614214/150‑1 (MQ=255)
                                         cAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAgg                                                                                                           <  1:60367/150‑1 (MQ=255)
                                          aGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGt                                                                                                          <  2:716844/150‑1 (MQ=255)
                                          aGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGt                                                                                                          >  2:754756/1‑150 (MQ=255)
                                           gCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTa                                                                                                         >  1:1033042/1‑150 (MQ=255)
                                           gCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTa                                                                                                         >  1:649319/1‑150 (MQ=255)
                                           gCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTa                                                                                                         >  1:1606756/1‑150 (MQ=255)
                                            cAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTaa                                                                                                        <  2:1608008/150‑1 (MQ=255)
                                             aaTATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAac                                                                                                       <  2:3848/150‑1 (MQ=255)
                                                atatTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTTCCAGGTAaccac                                                                                                    <  1:1586328/150‑1 (MQ=255)
                                                  atTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCAccc                                                                                                  <  1:60789/150‑1 (MQ=255)
                                                   ttGAATTTTCGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCAcccc                                                                                                 <  2:1544305/150‑1 (MQ=255)
                                                    tGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCa                                                                                                <  2:1412136/150‑1 (MQ=255)
                                                       aTTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGcc                                                                                             <  1:1310282/150‑1 (MQ=255)
                                                          tACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGt                                                                                          <  1:724627/150‑1 (MQ=255)
                                                            cGTGCTTTTGTTGGCCGGACAAAGCGTTTACGGCGCCTCCGGCATGTACAAAGCACACGTTGTTACCAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTag                                                                                        <  2:225867/150‑1 (MQ=255)
                                                               gCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGc                                                                                     >  2:257601/1‑150 (MQ=255)
                                                                cTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTaga                                                                                       >  1:611082/1‑147 (MQ=255)
                                                                cTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGACGGTAGAGCg                                                                                    >  1:1051124/1‑150 (MQ=255)
                                                                        ggCCTGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGTAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGa                                                                            <  1:210381/150‑1 (MQ=255)
                                                                            ggACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgtt                                                                        <  2:321706/150‑1 (MQ=255)
                                                                            ggACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgtt                                                                        >  2:761893/1‑150 (MQ=255)
                                                                               cAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgcc                                                                     >  1:686890/1‑150 (MQ=255)
                                                                               aaaaGCGTTTACGCCGCATCCGGCATGAACATAGCACACGTTGTTATCAATCAGAAATGCCGGGAATAAATCCCTGCATTTTCATAATCAGAAGTTGTAACGTACTACCATGTAACCTCCCCAGCCGGTAGAGCGAACGTTGAAgttggc                                                                     <  1:658067/149‑3 (MQ=255)
                                                                                 aaGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgt                                                                   >  1:115980/1‑150 (MQ=255)
                                                                                  aGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGcc                                                                                             <  2:1065192/123‑1 (MQ=255)
                                                                                  aGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGcc                                                                                             >  1:1065192/1‑123 (MQ=255)
                                                                                  aGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATATTCATAATCAGAAGTTGTAACATACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgtt                                                                  >  1:1457392/1‑150 (MQ=255)
                                                                                   gCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgttg                                                                 <  2:1606756/150‑1 (MQ=255)
                                                                                    cGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgttgc                                                                <  1:1255634/150‑1 (MQ=255)
                                                                                    cGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTCGATgttgccgttgc                                                                >  1:136624/1‑150 (MQ=255)
                                                                                     gTTTACGCGGCATCCGTCTTGAACAAAGGACACGTTGTTAACAATCAGAAATGCCGGTAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgttgcc                                                               <  2:98179/150‑1 (MQ=255)
                                                                                     gTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgttgcc                                                               <  2:1144287/150‑1 (MQ=255)
                                                                                       ttACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGa                                                             <  2:293598/150‑1 (MQ=255)
                                                                                       ttACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGa                                                             <  1:983588/150‑1 (MQ=255)
                                                                                        tACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGaa                                                            >  1:375519/1‑150 (MQ=255)
                                                                                        tACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGaa                                                            >  2:242687/1‑150 (MQ=255)
                                                                                         aCGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAag                                                           <  1:831426/150‑1 (MQ=255)
                                                                                              gcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttcag                                                      >  1:1521348/1‑150 (MQ=255)
                                                                                                ccccGCCAGGAACACAGCACACCTTGCTAACAATCAGAAAGGCCGGGAATACACCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttcagtt                                                    <  2:1615548/148‑1 (MQ=255)
                                                                                                  ccGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCt                                                  >  1:1264482/1‑150 (MQ=255)
                                                                                                  ccGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCt                                                  >  1:268325/1‑150 (MQ=255)
                                                                                                    ggCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGc                                                <  2:13485/150‑1 (MQ=255)
                                                                                                      cATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCaa                                              >  1:1473245/1‑149 (MQ=255)
                                                                                                       aTGAACAAAGCACTCGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtc                                             <  2:858647/150‑1 (MQ=255)
                                                                                                        tGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtcg                                            <  2:566230/150‑1 (MQ=255)
                                                                                                        tGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtcg                                            <  2:577883/150‑1 (MQ=255)
                                                                                                         gAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtcgt                                           <  1:257601/150‑1 (MQ=255)
                                                                                                          aaCAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtcgtc                                          >  1:491224/1‑150 (MQ=255)
                                                                                                              aaGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTc                                      <  1:1395008/150‑1 (MQ=255)
                                                                                                                 cacaCGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCAc                                   <  2:816681/150‑1 (MQ=255)
                                                                                                                         gttAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGaccacc                           <  2:1587905/150‑1 (MQ=255)
                                                                                                                            aaCAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTAACAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTACAGTTCTGCATCGTCGTTCCACTGACCACcgtc                        >  2:1208814/1‑150 (MQ=255)
                                                                                                                                 tCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGcc                   <  2:1682651/150‑1 (MQ=255)
                                                                                                                                      aaTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTaa              >  2:1421856/1‑150 (MQ=255)
                                                                                                                                      aaTGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGCCCACCGTCGTGCCAGTaa              >  1:458107/1‑150 (MQ=255)
                                                                                                                                       aTGCCGTGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCAGGTAGAGCGAACGTTGAAGTAGCCGTTGCCGAACTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAAc             >  1:80167/1‑150 (MQ=255)
                                                                                                                                       aTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGGTCTGCCTCGTCGTTCCACTGACCTCCGTCGTGCCAGTAAc             >  2:134237/1‑150 (MQ=255)
                                                                                                                                       aTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACATAATACCAGGTAACCACACCAGCAGGTAGAGCTAACCTTGAAGATGCCGTTGCCGAAGTTCAGGTCTGCATCGTCGTTCCACTGACCACCGTCGTGGCAGTAAc             >  1:148612/1‑150 (MQ=255)
                                                                                                                                       aTGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAAc             <  1:1372445/150‑1 (MQ=255)
                                                                                                                                          ccGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAg          <  1:907290/150‑1 (MQ=255)
                                                                                                                                          ccGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACAACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCTTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAg          <  1:901222/150‑1 (MQ=255)
                                                                                                                                           cGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGc         <  2:1525496/150‑1 (MQ=255)
                                                                                                                                            gggAATAAATACCGGCATTTTAATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCt        <  1:159277/150‑1 (MQ=255)
                                                                                                                                               aaTAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACg     >  2:1080587/1‑150 (MQ=255)
                                                                                                                                                aTAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGa    <  1:1721158/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACA  >  NC_000913/430963‑431258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: