Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,515,787 G→T 10.5% L43I (CTC→ATC) ‡ dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,515,7870GT10.5% 318.7 / 15.2 129L43I (CTC→ATC) ‡damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (59/52);  new base T (7/9);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

ATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCTT  >  NC_000913/3515638‑3515935
                                                                                                                                                     |                                                                                                                                                    
aTAGTAAACCGCGGCGCACTTTGGTTCGGGAACAACCAGCGCGCGTGCGGCCGGGACGTACTCCTCCGTACGCATCTTCACAATGTTATAGAGACTGATCAGGGCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgag                                                                                                                                                      <  2:836264/150‑1 (MQ=255)
  aGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATTGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaa                                                                                                                                                    >  2:707323/1‑150 (MQ=255)
   gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaaa                                                                                                                                                   >  2:136064/1‑150 (MQ=255)
   gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTAATGATATCGTCAAGGATATAACGAGAAAAGTCGGTGTTGAGaaa                                                                                                                                                   >  2:1040482/1‑150 (MQ=255)
     aaaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAaca                                                                                                                                                 >  2:918823/1‑150 (MQ=255)
        ccTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCg                                                                                                                                              <  2:984833/150‑1 (MQ=255)
           cGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTCTCAACACCGAct                                                                                                                                           <  1:972265/150‑2 (MQ=255)
            ggCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTCTCAACACCGActt                                                                                                                                          <  2:602974/150‑3 (MQ=255)
            ggCGAAATTTGTTTCGGTAACAAACAGCTCGCGTGCGGCCTGTACGTACTCTTCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTCTCAACACCGActt                                                                                                                                          <  2:1073617/150‑3 (MQ=255)
             gcgcAATTTGTTTCGGTAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCg                                                                                                                                         <  1:1521007/150‑1 (MQ=255)
             gcgcAATTTGTTTCGGGACCAAACAGCTCGCGTGCGTCCTGTACGTAATCATCAGTACGCATCTTCACAATGGTATAGAGACTGATCAGGTCGCTAGAGATAGCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCg                                                                                                                                         <  1:313535/150‑1 (MQ=255)
              cgcAATTTGTTTCGGGAACAAACAGCTCCCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTAAAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGATAAACACAGACACgg                                                                                                                                        >  1:1562908/1‑150 (MQ=255)
                cAATTTGTTTCGGTAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCa                                                                                                                                      <  1:896263/150‑1 (MQ=255)
                 aaTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGATATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAc                                                                                                                                     >  2:966467/1‑150 (MQ=255)
                   tttGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCCGTACGCATCTTCACAAGGTAATAGAGCCTGCTCAGGTGGCGTTTGCTATCGGCAAGGATATAACGAGAAAAGTCGGTGTTCAGAAACACCGACCCgggccct                                                                                                                                   >  2:274231/1‑145 (MQ=255)
                          cGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAg                                                                                                                            >  2:873405/1‑150 (MQ=255)
                          cGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGGCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAg                                                                                                                            <  2:3707/150‑1 (MQ=255)
                            ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACATTGTTTTAGAGACTGATCAGGTCGCTATTGATATCTGCAAGGATATAACGAGAAACGTCGGTGTTGAGAAACACCGTCACGGCACCTACAAAAGGc                                                                                                                          >  2:187854/1‑150 (MQ=255)
                             gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCt                                                                                                                         <  2:1067783/150‑1 (MQ=255)
                             gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCt                                                                                                                         >  1:1434340/1‑150 (MQ=255)
                              aacaaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTc                                                                                                                        >  2:1217336/1‑150 (MQ=255)
                                  aacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTCCGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGAGAAAACAAGAACAGTCGCTGGTGCTAAACACCGAGCCGGCACCTACAAGAGCCTCCAcc                                                                                                                    >  1:89701/1‑150 (MQ=255)
                                  aacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAcc                                                                                                                    <  1:1601064/150‑1 (MQ=255)
                                   acaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCa                                                                                                                   <  2:37441/150‑1 (MQ=255)
                                           cgTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGCAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCg                                                                                                           >  1:224737/1‑150 (MQ=255)
                                            gTGCGGCGTGTACGTAGTCATAAGTAGGCATATTCAAAATGTTATTGAGACTGTTCTGGTGGCTTTTTATATCGGCAATGATATAACGACAAACGGCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGc                                                                                                          <  2:1307958/150‑1 (MQ=255)
                                            gTGCGGCCTGTACGTAATAATCAGTACGAATCTTCAAAATGTTATAGAGACTGATCAGGTCGCTATTTATATCGGAAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGc                                                                                                          <  1:1697056/150‑1 (MQ=255)
                                               cGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCt                                                                                                       >  1:1710627/1‑150 (MQ=255)
                                                   cTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                                                                                                   <  1:918823/150‑1 (MQ=255)
                                                       acgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGCAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTAGGGCaaa                                                                                               >  1:1338731/1‑150 (MQ=255)
                                                        cgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTTGCCCAAGGGCGAAt                                                                                              <  1:415958/150‑1 (MQ=255)
                                                          tacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGGCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGc                                                                                            <  1:718060/150‑1 (MQ=255)
                                                            cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                                                                          >  1:1147064/1‑150 (MQ=255)
                                                             tcatcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGt                                                                                         >  2:1242416/1‑150 (MQ=255)
                                                              catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt                                                                                        >  1:781937/1‑150 (MQ=255)
                                                              catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt                                                                                        >  2:1498703/1‑150 (MQ=255)
                                                              catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCAATTGATATCGGCAAGGATATAACGAGCAAAGTCGGAGTTGAGAAACACCGACCCGGCACCGACAAAAGGCTCAACCAGACATTCGCACTTGGGCAAATGCCGtt                                                                                        >  1:164957/1‑150 (MQ=255)
                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTa                                                                                      >  1:1400681/1‑150 (MQ=255)
                                                                   gTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTAGGGCAAATGCCGTTTAata                                                                                   >  2:1512694/1‑150 (MQ=255)
                                                                      cGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatca                                                                                >  2:466450/1‑150 (MQ=255)
                                                                      cGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatca                                                                                <  1:258881/150‑1 (MQ=255)
                                                                        cATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcatc                                                                              <  2:717047/150‑1 (MQ=255)
                                                                        cATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcatc                                                                              >  2:1448292/1‑150 (MQ=255)
                                                                           cTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAg                                                                           >  2:1229593/1‑150 (MQ=255)
                                                                            ttCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                                                                          <  1:768601/150‑1 (MQ=255)
                                                                            ttCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                                                                          <  2:777389/150‑1 (MQ=255)
                                                                            ttCACAATGTTATAGAGACTGATCAGGTCGATATTGATATCGGCAAGGATTTAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                                                                          <  2:658611/150‑1 (MQ=255)
                                                                               acaATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAACGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCACCCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                                                                       >  2:272239/1‑150 (MQ=255)
                                                                               aaaaTTTTATAGAGACTTATCAGGTCGCTTTTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                                                                       <  1:376075/148‑1 (MQ=255)
                                                                                caATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                                                                      >  1:1144584/1‑150 (MQ=255)
                                                                                caATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                                                                      <  1:934133/150‑1 (MQ=255)
                                                                                      tataGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACt                                                                >  1:1303061/1‑150 (MQ=255)
                                                                                          gagaCTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAATTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcc                                                            <  1:966467/150‑1 (MQ=255)
                                                                                          gagaCTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcc                                                            <  1:1217336/150‑1 (MQ=255)
                                                                                            gaCTGATCAGGGCGCTATTGATCTCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcccc                                                          <  2:103081/150‑1 (MQ=255)
                                                                                               tGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTg                                                       <  1:707323/150‑1 (MQ=255)
                                                                                                gATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGc                                                      >  1:396843/1‑150 (MQ=255)
                                                                                                gATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGc                                                      <  1:136064/150‑1 (MQ=255)
                                                                                                gATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGc                                                      >  1:540145/1‑150 (MQ=255)
                                                                                                  tCAGGGCGCTATTGATCTCGGCAAGGATATAACGAGAAACGTCGGGGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGccc                                                    <  1:868302/150‑1 (MQ=255)
                                                                                                   cAGTTCTCTATTGATATCTGCAAGGATATAACGAGAAAAGTAGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCa                                                   <  2:1148261/150‑1 (MQ=255)
                                                                                                      gTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACtt                                                >  2:275332/1‑150 (MQ=255)
                                                                                                       tCGCTATTGATATCGGCAAGTATATAACGAGAAAAGGCGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTc                                               <  1:1472213/150‑1 (MQ=255)
                                                                                                          cTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCaaa                                            >  2:197026/1‑150 (MQ=255)
                                                                                                           tATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCaaaa                                           >  2:196234/1‑150 (MQ=255)
                                                                                                            aTTGATATCGGCAAGGATATAACGAGAAAAGTCTGAGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCAGTTTAATATCATCAAGCAGGGTATACTTGCCCCCTGCCCACTTCaaaaa                                          <  1:1246164/150‑1 (MQ=255)
                                                                                                            aTTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCaaaaa                                          >  2:514090/1‑150 (MQ=255)
                                                                                                              tGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAg                                        <  2:1722538/150‑1 (MQ=255)
                                                                                                               tatatCGGCATGTATATAACTCGAAAAGTCGTTGTTGCTAACCACCGACCCGGCACCTACAAAATGCGCATCCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAgc                                       <  1:863887/149‑1 (MQ=255)
                                                                                                                atatCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAgcg                                      >  2:1507140/1‑150 (MQ=255)
                                                                                                                 tatCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAgcgc                                     >  2:1674834/1‑150 (MQ=255)
                                                                                                                  atCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAACCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCATGGGCAAAAGCCCTTTAATATCATCAAGCAGGGTATACTTGCCCCCTGCACACTTCAAAAAAgcgag                                    >  2:875584/1‑148 (MQ=255)
                                                                                                                     ggCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCACACTTCAAAAAAGAGAGAtt                                 >  2:1535711/1‑150 (MQ=255)
                                                                                                                       cAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGAtttt                               <  2:1147064/150‑1 (MQ=255)
                                                                                                                       cAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGAtttt                               >  2:342697/1‑150 (MQ=255)
                                                                                                                         actacataACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTttc                             <  2:621265/145‑1 (MQ=255)
                                                                                                                          ggATATAACGAGAAAAGTCGGTGTGGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTttct                            <  1:445924/150‑1 (MQ=255)
                                                                                                                           gATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTttctt                           <  2:1154941/150‑1 (MQ=255)
                                                                                                                            atatAACGAGAAAAGTCGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTttcttc                          <  1:637954/150‑1 (MQ=255)
                                                                                                                             tataACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCCGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCa                         >  2:340701/1‑150 (MQ=255)
                                                                                                                               taACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATg                       >  1:1650577/1‑150 (MQ=255)
                                                                                                                                aactataaatGTCGGTGTGGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGc                      <  1:1314129/144‑1 (MQ=255)
                                                                                                                                aaCGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGc                      <  2:758509/150‑1 (MQ=255)
                                                                                                                                 actagaAAAGGCGGTGTTGAGAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCt                     <  2:1298185/147‑1 (MQ=255)
                                                                                                                                    agaAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGac                  <  1:1203566/150‑1 (MQ=255)
                                                                                                                                       cgggTCGGTGTTTCTCAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGGTGactaa               >  2:297155/4‑150 (MQ=255)
                                                                                                                                         ggTCGGTGTTTCTCAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGactaact             >  2:1275578/2‑150 (MQ=255)
                                                                                                                                         aGTCGGTGTTGAGAAACACCGACCCGGCACCTATAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGactaact             <  2:1664396/150‑1 (MQ=255)
                                                                                                                                         aGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGactaact             >  2:748906/1‑150 (MQ=255)
                                                                                                                                          gTCGGTGTTTCTCAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAAAAACATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGactaacga            >  2:1535552/1‑148 (MQ=255)
                                                                                                                                          gTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTACAAAAAAGCGCGATTATTCTTCATGCTGactaacta            >  1:863892/1‑150 (MQ=255)
                                                                                                                                              gttttGATAAAAACCGAACCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCTCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTa        <  2:1562908/147‑1 (MQ=255)
                                                                                                                                              gtgtTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTa        >  1:1707856/1‑150 (MQ=255)
                                                                                                                                                gtTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTaca      >  1:442338/1‑150 (MQ=255)
                                                                                                                                                 ttGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGTGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTacac     >  2:818619/1‑150 (MQ=255)
                                                                                                                                                 ttGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTacac     >  2:1019743/1‑150 (MQ=255)
                                                                                                                                                  tGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACAcc    >  1:385612/1‑150 (MQ=255)
                                                                                                                                                   gagaAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCt   >  2:815130/1‑150 (MQ=255)
                                                                                                                                                    agaAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCtt  >  2:1380906/1‑150 (MQ=255)
                                                                                                                                                    agaAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCtt  >  1:1162123/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCTT  >  NC_000913/3515638‑3515935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: