Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,515,788 A→C 6.9% F42L (TTT→TTG dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,515,7880AC 6.9% 368.7 / 12.6 126F42L (TTT→TTGdamDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (57/60);  new base C (6/3);  total (63/63)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 135 total aligned reads displayed.

AGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCTTCT  >  NC_000913/3515640‑3515937
                                                                                                                                                    |                                                                                                                                                     
aGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATTGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaa                                                                                                                                                      >  2:707323/1‑150 (MQ=255)
 gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaaa                                                                                                                                                     >  2:136064/1‑150 (MQ=255)
 gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTAATGATATCGTCAAGGATATAACGAGAAAAGTCGGTGTTGAGaaa                                                                                                                                                     >  2:1040482/1‑150 (MQ=255)
   aaaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAaca                                                                                                                                                   >  2:918823/1‑150 (MQ=255)
      ccTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCg                                                                                                                                                <  2:984833/150‑1 (MQ=255)
         cGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTCTCAACACCGAct                                                                                                                                             <  1:972265/150‑2 (MQ=255)
          ggCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTCTCAACACCGActt                                                                                                                                            <  2:602974/150‑3 (MQ=255)
          ggCGAAATTTGTTTCGGTAACAAACAGCTCGCGTGCGGCCTGTACGTACTCTTCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTCTCAACACCGActt                                                                                                                                            <  2:1073617/150‑3 (MQ=255)
           gcgcAATTTGTTTCGGTAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCg                                                                                                                                           <  1:1521007/150‑1 (MQ=255)
           gcgcAATTTGTTTCGGGACCAAACAGCTCGCGTGCGTCCTGTACGTAATCATCAGTACGCATCTTCACAATGGTATAGAGACTGATCAGGTCGCTAGAGATAGCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCg                                                                                                                                           <  1:313535/150‑1 (MQ=255)
            cgcAATTTGTTTCGGGAACAAACAGCTCCCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTAAAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGATAAACACAGACACgg                                                                                                                                          >  1:1562908/1‑150 (MQ=255)
              cAATTTGTTTCGGTAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCa                                                                                                                                        <  1:896263/150‑1 (MQ=255)
               aaTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGATATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAc                                                                                                                                       >  2:966467/1‑150 (MQ=255)
                 tttGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCCGTACGCATCTTCACAAGGTAATAGAGCCTGCTCAGGTGGCGTTTGCTATCGGCAAGGATATAACGAGAAAAGTCGGTGTTCAGAAACACCGACCCgggccct                                                                                                                                     >  2:274231/1‑145 (MQ=255)
                   tGTTTCGGGAACAAATAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTAc                                                                                                                                   >  1:1662419/1‑150 (MQ=255)
                        cGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAg                                                                                                                              >  2:873405/1‑150 (MQ=255)
                        cGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGGCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAg                                                                                                                              <  2:3707/150‑1 (MQ=255)
                          ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACATTGTTTTAGAGACTGATCAGGTCGCTATTGATATCTGCAAGGATATAACGAGAAACGTCGGTGTTGAGAAACACCGTCACGGCACCTACAAAAGGc                                                                                                                            >  2:187854/1‑150 (MQ=255)
                          ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGc                                                                                                                            <  1:190197/150‑1 (MQ=255)
                          ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGc                                                                                                                            <  1:354145/150‑1 (MQ=255)
                           gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCt                                                                                                                           >  1:1434340/1‑150 (MQ=255)
                           gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCt                                                                                                                           <  2:1067783/150‑1 (MQ=255)
                                aacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTCCGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGAGAAAACAAGAACAGTCGCTGGTGCTAAACACCGAGCCGGCACCTACAAGAGCCTCCAcc                                                                                                                      >  1:89701/1‑150 (MQ=255)
                                aacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAcc                                                                                                                      <  2:732646/150‑1 (MQ=255)
                                aacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAcc                                                                                                                      <  1:1601064/150‑1 (MQ=255)
                                 acaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCa                                                                                                                     <  2:37441/150‑1 (MQ=255)
                                          gTGCGGCGTGTACGTAGTCATAAGTAGGCATATTCAAAATGTTATTGAGACTGTTCTGGTGGCTTTTTATATCGGCAATGATATAACGACAAACGGCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGc                                                                                                            <  2:1307958/150‑1 (MQ=255)
                                          gTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGc                                                                                                            <  1:398498/150‑1 (MQ=255)
                                             cGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCt                                                                                                         >  1:1710627/1‑150 (MQ=255)
                                               gCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATAAAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTg                                                                                                       <  2:629679/150‑1 (MQ=255)
                                                 cTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                                                                                                     <  1:833680/150‑1 (MQ=255)
                                                 cTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                                                                                                     <  1:918823/150‑1 (MQ=255)
                                                  tGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGc                                                                                                    >  2:1203566/1‑150 (MQ=255)
                                                     acgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGCAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTAGGGCaaa                                                                                                 >  1:1338731/1‑150 (MQ=255)
                                                          cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                                                                            >  1:1147064/1‑150 (MQ=255)
                                                           tcatcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGt                                                                                           >  2:1242416/1‑150 (MQ=255)
                                                            catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt                                                                                          >  1:781937/1‑150 (MQ=255)
                                                            catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt                                                                                          >  2:1498703/1‑150 (MQ=255)
                                                              tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTa                                                                                        >  1:1400681/1‑150 (MQ=255)
                                                               caGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATAAAACGAGAAAAGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTaa                                                                                       <  1:707863/150‑1 (MQ=255)
                                                                 gTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTAGGGCAAATGCCGTTTAata                                                                                     >  2:1512694/1‑150 (MQ=255)
                                                                    cGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatca                                                                                  <  1:258881/150‑1 (MQ=255)
                                                                      cATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcatc                                                                                >  2:1448292/1‑150 (MQ=255)
                                                                         cTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAg                                                                             >  2:1229593/1‑150 (MQ=255)
                                                                          ttCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                                                                            <  1:768601/150‑1 (MQ=255)
                                                                          ttCACAATGTTATAGAGACTGATCAGGTCGATATTGATATCGGCAAGGATTTAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGc                                                                            <  2:658611/150‑1 (MQ=255)
                                                                             acaATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAACGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCACCCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                                                                         >  2:272239/1‑150 (MQ=255)
                                                                             acaATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                                                                         <  1:1586314/150‑1 (MQ=255)
                                                                              caATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                                                                        <  1:934133/150‑1 (MQ=255)
                                                                              caATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                                                                        >  1:1144584/1‑150 (MQ=255)
                                                                                    tataGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACt                                                                  >  1:1303061/1‑150 (MQ=255)
                                                                                        gagaCTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAATTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcc                                                              <  1:966467/150‑1 (MQ=255)
                                                                                        gagaCTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcc                                                              <  1:1217336/150‑1 (MQ=255)
                                                                                          gaCTGATCAGGGCGCTATTGATCTCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcccc                                                            <  2:103081/150‑1 (MQ=255)
                                                                                             tGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTg                                                         <  1:707323/150‑1 (MQ=255)
                                                                                              gATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGc                                                        >  1:396843/1‑150 (MQ=255)
                                                                                              gATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGc                                                        <  1:136064/150‑1 (MQ=255)
                                                                                                tCAGGGCGCTATTGATCTCGGCAAGGATATAACGAGAAACGTCGGGGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGccc                                                      <  1:868302/150‑1 (MQ=255)
                                                                                                 cAGTTCTCTATTGATATCTGCAAGGATATAACGAGAAAAGTAGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCa                                                     <  2:1148261/150‑1 (MQ=255)
                                                                                                    gTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACtt                                                  >  2:275332/1‑150 (MQ=255)
                                                                                                     tCGCTATTGATATCGGCAAGTATATAACGAGAAAAGGCGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTc                                                 <  1:1472213/150‑1 (MQ=255)
                                                                                                        cTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCaaa                                              >  2:197026/1‑150 (MQ=255)
                                                                                                         tATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCaaaa                                             >  2:196234/1‑150 (MQ=255)
                                                                                                          aTTGATATCGGCAAGGATATAACGAGAAAAGTCTGAGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCAGTTTAATATCATCAAGCAGGGTATACTTGCCCCCTGCCCACTTCaaaaa                                            <  1:1246164/150‑1 (MQ=255)
                                                                                                          aTTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCaaaaa                                            >  2:514090/1‑150 (MQ=255)
                                                                                                            tGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAg                                          <  2:1722538/150‑1 (MQ=255)
                                                                                                              atatCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAgcg                                        >  2:1507140/1‑150 (MQ=255)
                                                                                                               tatCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAgcgc                                       >  2:1674834/1‑150 (MQ=255)
                                                                                                                atCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAACCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCATGGGCAAAAGCCCTTTAATATCATCAAGCAGGGTATACTTGCCCCCTGCACACTTCAAAAAAgcgag                                      >  2:875584/1‑148 (MQ=255)
                                                                                                                   ggCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCACACTTCAAAAAAGAGAGAtt                                   >  2:1535711/1‑150 (MQ=255)
                                                                                                                     cAAGTATATAACTAGAAAAGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGAtttt                                 <  2:366724/150‑1 (MQ=255)
                                                                                                                        ggATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTttct                              <  2:781937/150‑1 (MQ=255)
                                                                                                                        ggATATAACGAGAAAAGTCGGTGTGGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTttct                              <  1:445924/150‑1 (MQ=255)
                                                                                                                         gATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTttctt                             <  2:1154941/150‑1 (MQ=255)
                                                                                                                          atatAACGAGAAAAGTCGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTttcttc                            <  1:637954/150‑1 (MQ=255)
                                                                                                                           tataACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCCGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCa                           >  2:340701/1‑150 (MQ=255)
                                                                                                                             taACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATg                         >  1:1650577/1‑150 (MQ=255)
                                                                                                                              aactataaatGTCGGTGTGGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGc                        <  1:1314129/144‑1 (MQ=255)
                                                                                                                              aaCGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGc                        <  2:758509/150‑1 (MQ=255)
                                                                                                                               actagaAAAGGCGGTGTTGAGAAACCCCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCt                       <  2:1298185/147‑1 (MQ=255)
                                                                                                                                  agaAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGac                    <  1:1203566/150‑1 (MQ=255)
                                                                                                                                     cgggTCGGTGTTTCTCAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGGTGactaa                 >  2:297155/4‑150 (MQ=255)
                                                                                                                                       aGTCGGTGTTGAGAAACACCGACCCGGCACCTATAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGactaact               <  2:1664396/150‑1 (MQ=255)
                                                                                                                                       aGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGactaact               >  2:748906/1‑150 (MQ=255)
                                                                                                                                        gTCGGTGTTTCTCAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGactaacta              >  2:480825/1‑150 (MQ=255)
                                                                                                                                        gTCGGTGTTTCTCAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAAAAACATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGactaacga              >  2:1535552/1‑148 (MQ=255)
                                                                                                                                        gTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTACAAAAAAGCGCGATTATTCTTCATGCTGactaacta              >  1:863892/1‑150 (MQ=255)
                                                                                                                                            gttttGATAAAAACCGAACCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCTCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTa          <  2:1562908/147‑1 (MQ=255)
                                                                                                                                            gtgtTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTa          >  2:1243508/1‑150 (MQ=255)
                                                                                                                                            gtgtTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTa          >  1:1707856/1‑150 (MQ=255)
                                                                                                                                              gtTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTaca        >  1:442338/1‑150 (MQ=255)
                                                                                                                                              gtTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATATTTCTTCATGCTGACTAACTTATTaca        >  2:1113884/1‑150 (MQ=255)
                                                                                                                                               ttGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGTGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTacac       >  2:818619/1‑150 (MQ=255)
                                                                                                                                               ttGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTacac       >  2:1019743/1‑150 (MQ=255)
                                                                                                                                                tGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACAcc      >  1:385612/1‑150 (MQ=255)
                                                                                                                                                 gagaAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCt     >  1:515047/1‑150 (MQ=255)
                                                                                                                                                 gagaAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCt     >  2:815130/1‑150 (MQ=255)
                                                                                                                                                  agaAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCtt    >  2:1380906/1‑150 (MQ=255)
                                                                                                                                                  agaAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCtt    >  1:1162123/1‑150 (MQ=255)
                                                                                                                                                    aaaCACCGACCCGGAACCTACAAAAGGCTCACCCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCTTCACGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCTtct  <  1:1529048/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCACTTCAAAAAAGCGCGATTTTTCTTCATGCTGACTAACTAATTACACCTTCT  >  NC_000913/3515640‑3515937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: