Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,544,689 A→T 6.2% R52R (CGT→CGA bioH ← pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,544,6890AT 6.2% 315.7 / 7.2 117R52R (CGT→CGAbioHpimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base A (66/43);  new base T (3/5);  total (69/48)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

Only 100 of 119 total aligned reads displayed.

AGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGCCACCAGATGTT  >  NC_000913/3544540‑3544838
                                                                                                                                                     |                                                                                                                                                     
aGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTa                                                                                                                                                       <  1:1010624/150‑1 (MQ=255)
    ccTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGcc                                                                                                                                                   <  2:562738/150‑1 (MQ=255)
       gAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGa                                                                                                                                                >  2:1309624/1‑150 (MQ=255)
           acGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCCCCAAATCCCCGGCTACGCCCGAAGcc                                                                                                                                            >  1:300318/1‑150 (MQ=255)
           acGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGcc                                                                                                                                            >  1:1273451/1‑150 (MQ=255)
            cGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCCCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCg                                                                                                                                           >  2:643444/1‑150 (MQ=255)
            cGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCg                                                                                                                                           >  2:657005/1‑150 (MQ=255)
             gCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCGGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCgg                                                                                                                                          >  1:1168173/1‑150 (MQ=255)
              cTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGGCTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCggg                                                                                                                                         >  1:557497/1‑150 (MQ=255)
              cTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCggg                                                                                                                                         >  1:1669106/1‑150 (MQ=255)
              cTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCggg                                                                                                                                         >  1:32731/1‑150 (MQ=255)
               tCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTTCGGGCGTAGCCgggg                                                                                                                                        <  2:579201/150‑2 (MQ=255)
               tCGGGATGGGTTAACGCAATCTGGCTTGCCACCACCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAAACCCCGGCGTCGGGCGTAGCCgggg                                                                                                                                        <  2:1461837/150‑2 (MQ=255)
                cGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTTCGGGCGTAGCCgggga                                                                                                                                       <  2:1685231/150‑3 (MQ=255)
                 gggATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAAAGGCTTTATCAGGTGCCTGTTGCAGCCCGGCTACGGCCATATCAGCAAGTGACAGCGCACCAAAACCCCGGCAACGCCCGACGCCGGGccc                                                                                                                                      >  1:603399/1‑148 (MQ=255)
                  ggATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGCCTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGCCAGCGCACCACATCCCCGGCTACGCCCGAAGCCGGGCAgg                                                                                                                                     >  1:1224809/1‑150 (MQ=255)
                  ggATGGGTTAACGCAATCTGGCTTGCCACAAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAgg                                                                                                                                     >  1:153440/1‑150 (MQ=255)
                      gggTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTcaa                                                                                                                                 <  1:510675/150‑1 (MQ=255)
                          tAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAg                                                                                                                             >  1:458128/1‑150 (MQ=255)
                           aaCGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAgg                                                                                                                            <  2:1672898/150‑1 (MQ=255)
                           aaCGCAATCTGGCTTGCCACCAGCCAGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAgg                                                                                                                            <  1:999839/150‑1 (MQ=255)
                            aCGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGt                                                                                                                           >  1:1433285/1‑150 (MQ=255)
                                   cTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGt                                                                                                                    >  2:1710847/1‑150 (MQ=255)
                                   cTGGCTTGCCACAAGCCCGCCCAGACTCCCGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCACATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCgg                                                                                                                    >  1:1111663/1‑149 (MQ=255)
                                      gCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGAACGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACACGGTGCAGCGTaaa                                                                                                                 >  1:1727575/1‑150 (MQ=255)
                                        ttGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCCGCACGGCTTCGGCCAGATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCACCAAGGTGCAGCGTAAAAt                                                                                                               >  2:112133/1‑150 (MQ=255)
                                           ccaccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCg                                                                                                            >  2:377606/1‑150 (MQ=255)
                                           ccaccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGCTCAACAAGGTGCAGCGTAAAATGCg                                                                                                            >  2:154888/1‑150 (MQ=255)
                                           ccaccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGAGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCg                                                                                                            >  1:565491/1‑150 (MQ=255)
                                            caccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCTGGTCAACAAGGTGCAGCGTAAAATGCGa                                                                                                           >  2:66722/1‑150 (MQ=255)
                                            caccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGa                                                                                                           >  1:414229/1‑150 (MQ=255)
                                             accaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAg                                                                                                          <  1:26400/150‑1 (MQ=255)
                                               caGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCt                                                                                                        <  2:818347/150‑1 (MQ=255)
                                               caGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCt                                                                                                        >  2:1395977/1‑150 (MQ=255)
                                                aGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAACTGCGAGCTa                                                                                                       >  1:717314/1‑150 (MQ=255)
                                                aGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTa                                                                                                       >  2:316607/1‑150 (MQ=255)
                                                 gcccgcccAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTaa                                                                                                      >  1:657309/1‑150 (MQ=255)
                                                 gcccgcccAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGACATATCAGAAAGTGCCAGCGCCCCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGTGCTaa                                                                                                      >  2:388037/1‑150 (MQ=255)
                                                      cccAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGGTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTcc                                                                                                 <  1:1630075/150‑1 (MQ=255)
                                                       ccAGCCACCAGCCTAACCAAATGGCTTTATCTGGGGCCTGTTGCAGCACGGCTTCGGCCATATCAGCATGCGACAGCGCACCAAATCCGCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGGGCAGCGTAAAATGCGAGCTAAGTTCCt                                                                                                <  2:430709/150‑1 (MQ=255)
                                                         aGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCg                                                                                              >  2:586191/1‑150 (MQ=255)
                                                         aGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCg                                                                                              <  2:1465861/150‑1 (MQ=255)
                                                           aCTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTc                                                                                            <  2:153440/150‑1 (MQ=255)
                                                            cTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCa                                                                                           >  2:1274560/1‑150 (MQ=255)
                                                               cAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATg                                                                                        <  1:975358/150‑1 (MQ=255)
                                                                aGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGc                                                                                       <  1:1466819/150‑1 (MQ=255)
                                                                   cTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAAc                                                                                    <  1:188713/150‑1 (MQ=255)
                                                                    tacacAAATGGCTTTATCAGGAGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTAAATGCAACg                                                                                   <  1:1308626/146‑1 (MQ=255)
                                                                         aaaTGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGTTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCcaca                                                                              <  1:177389/150‑1 (MQ=255)
                                                                          aaTGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCTGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCacac                                                                             <  1:889201/150‑1 (MQ=255)
                                                                          aaTGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCacac                                                                             >  1:1580078/1‑150 (MQ=255)
                                                                               cTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCgg                                                                        <  2:414229/150‑1 (MQ=255)
                                                                               cTTTAGCAGGTGCCGGGTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGGCAATGCAACGCCACACTTCgg                                                                        <  2:277845/150‑1 (MQ=255)
                                                                                tttATCATGTGCCTGTTGCACGACGGCTTCGGCCATATCATCAACTGTCAGCCCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGc                                                                       <  2:225156/150‑1 (MQ=255)
                                                                                tttATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCCGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGc                                                                       >  1:1311474/1‑150 (MQ=255)
                                                                                    tCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGAGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTc                                                                   <  2:565491/150‑1 (MQ=255)
                                                                                    tCAGGTGCCTGGTGCATCACGGCTTCGGCCATATCAGCATGTGAGAGCGGACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGTTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCTCACTTCGGCATTc                                                                   <  1:1420842/150‑1 (MQ=255)
                                                                                     cAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCa                                                                  <  1:1024250/150‑1 (MQ=255)
                                                                                         tGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTcc                                                              >  1:1221503/1‑150 (MQ=255)
                                                                                          gCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTccc                                                             >  2:234223/1‑150 (MQ=255)
                                                                                             tGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACACCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCAt                                                          >  1:1109136/1‑150 (MQ=255)
                                                                                              gTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCCATGAAACGCCAGCCTTCGGCATTCAGTCCCCATc                                                         >  1:1522233/1‑150 (MQ=255)
                                                                                                tGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCg                                                       >  2:1547948/1‑150 (MQ=255)
                                                                                                  cagcaCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCACCAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTg                                                     >  1:1651001/1‑150 (MQ=255)
                                                                                                    gcaCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgca                                                   >  1:206073/1‑150 (MQ=255)
                                                                                                    gcaCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgca                                                   <  1:316607/150‑1 (MQ=255)
                                                                                                      aCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCACTGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgcagc                                                 >  1:1554038/1‑150 (MQ=255)
                                                                                                      aCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgcagc                                                 <  1:1184170/150‑1 (MQ=255)
                                                                                                        ggCGTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGGCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAAAGCCACACTTCGGCTTTCAGTCCCCATCCGTgcagcag                                               <  2:1232102/150‑1 (MQ=255)
                                                                                                         ccTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGAGCAGCGTAAAATGCGAGCAAAGTTACACGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgcagcagc                                              >  2:1125590/2‑150 (MQ=255)
                                                                                                          cTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgcagcagca                                             <  1:1464078/150‑1 (MQ=255)
                                                                                                           ttCGGCCATATCAGCAAGTGACTGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGcac                                            <  2:1091137/150‑1 (MQ=255)
                                                                                                              ggCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCCCACTTCGGCATTCAGTCCCCATCCGTGCAGCAACACAAg                                         >  1:1142164/1‑150 (MQ=255)
                                                                                                               gCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGa                                        >  2:535084/1‑150 (MQ=255)
                                                                                                                 cATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATg                                      >  1:1362561/1‑150 (MQ=255)
                                                                                                                  atatCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGa                                     <  2:1587212/150‑1 (MQ=255)
                                                                                                                     tCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACa                                  <  1:1499893/150‑1 (MQ=255)
                                                                                                                      cAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACAt                                 >  2:1008362/1‑150 (MQ=255)
                                                                                                                        gCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTc                               <  1:1678269/150‑1 (MQ=255)
                                                                                                                             tGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCATCGCCACACTTAGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTACACTg                          >  2:300797/1‑150 (MQ=255)
                                                                                                                                cAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGAcc                       >  2:1406280/1‑150 (MQ=255)
                                                                                                                                  gcgcACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCtt                     >  1:424362/1‑150 (MQ=255)
                                                                                                                                   cgcACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCttt                    >  1:766652/1‑150 (MQ=255)
                                                                                                                                    gcACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTg                   >  2:1137446/1‑150 (MQ=255)
                                                                                                                                    gcACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTg                   <  1:154888/150‑1 (MQ=255)
                                                                                                                                    gcACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCATGACCTTTg                   >  1:927240/1‑150 (MQ=255)
                                                                                                                                       ccAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTc                >  2:1604904/1‑150 (MQ=255)
                                                                                                                                          cctgcccGGCTTCGGGCGTAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGc             >  2:322507/5‑150 (MQ=255)
                                                                                                                                           ctgcccGGCTTCGGGCGTAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcc            >  1:374440/4‑150 (MQ=255)
                                                                                                                                           aTCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcc            <  2:657309/150‑1 (MQ=255)
                                                                                                                                           aTCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcc            <  1:525016/150‑1 (MQ=255)
                                                                                                                                           aTCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcc            <  1:649875/150‑1 (MQ=255)
                                                                                                                                            tgcccGGCTTCGGGCGTAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcgc           >  1:926035/3‑148 (MQ=255)
                                                                                                                                             ccccGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGccac          <  1:414601/150‑1 (MQ=255)
                                                                                                                                              cccGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGccacc         >  1:1099260/1‑150 (MQ=255)
                                                                                                                                              cccGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGccacc         >  1:682546/1‑150 (MQ=255)
                                                                                                                                              cccGGCTACGCCCGAAGCAGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGccacc         <  2:1074775/150‑1 (MQ=255)
                                                                                                                                               ccGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGccacca        >  2:592126/1‑150 (MQ=255)
                                                                                                                                                 ggCTACGCGCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCACCATCCGTTCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGCCACCAGa      <  2:1082405/150‑1 (MQ=255)
                                                                                                                                                    tACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGGCTGCCACCAGATGt   <  1:724784/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGCCACCAGATGTT  >  NC_000913/3544540‑3544838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: