Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,506 C→G 5.2% R194P (CGT→CCT) ‡ ftsX ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,5060CG 5.2% 313.1 / 6.5 96R194P (CGT→CCT) ‡ftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (45/45);  new base G (3/2);  total (49/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

CACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGA  >  NC_000913/3601357‑3601655
                                                                                                                                                     |                                                                                                                                                     
cacTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATAc                                                                                                                                                       <  2:1111197/150‑1 (MQ=255)
cacTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGATAATCTGCGTGATAc                                                                                                                                                       >  1:646493/1‑150 (MQ=255)
   tGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAt                                                                                                                                                    <  1:683058/150‑1 (MQ=255)
   tGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAt                                                                                                                                                    <  1:435033/150‑1 (MQ=255)
   tGTTAACGATGACGATGAACACGGCCGCCACCATCAACACTCCGAGCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAt                                                                                                                                                    <  2:894830/150‑1 (MQ=255)
     ttACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCa                                                                                                                                                  >  1:403291/1‑150 (MQ=255)
       aCCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACg                                                                                                                                                >  1:1213893/1‑150 (MQ=255)
          gATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCACCGCCGCCAGACGGGCAAACCAGCTGTCATCCCTCCGCACTGCGGCAATGCCGTTAATCTGCGTGATACGATCACGCAg                                                                                                                                             >  1:73279/1‑150 (MQ=255)
          gATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATCGTATCACGCAg                                                                                                                                             <  1:1347452/150‑1 (MQ=255)
          gATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAg                                                                                                                                             >  2:938460/1‑150 (MQ=255)
           ttGACGAGGAACACGGCCGCCACCATCAACACGCCGATCAGCGCCGAAACGCGCCCGACCCGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGc                                                                                                                                            <  2:115674/149‑1 (MQ=255)
             gACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATCGTATCACGCAgatt                                                                                                                                          <  2:1632189/150‑4 (MQ=255)
                gAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCTCTTCGTCAATGCCGTTAATCTGCGTGCTACGATCACGCAGCGTAtt                                                                                                                                       >  1:1211450/1‑150 (MQ=255)
                gAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTAtt                                                                                                                                       >  1:307367/1‑150 (MQ=255)
                  ggAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCa                                                                                                                                     >  2:801358/1‑150 (MQ=255)
                  ggAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAGCACGCAGCGTATTCa                                                                                                                                     >  2:1002525/1‑150 (MQ=255)
                   gAACACGGCCGCCTCCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAg                                                                                                                                    <  2:775908/150‑1 (MQ=255)
                        cGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCCCTTCGTCACTGCCGTTAATCTGCGTGATACGATCCCGCAGCGTAGTCAGTGCtt                                                                                                                               >  1:706765/1‑150 (MQ=255)
                        cGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGCTCAACGCCGCCAGACGGGCAAACCAGCTGTCAGCCATCCGCTCTTCCTCAATGCCGTTAATCTGCGTGCTACGATCACGCAGCGTATTCAGTGAtt                                                                                                                               >  1:451115/1‑150 (MQ=255)
                          gccgccACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCCTCCGCACTTCGTCAATGCCGTTACTCTGCGTGATACGCTCACGCAGCGGATTCAGTGATTcc                                                                                                                             >  1:300334/1‑150 (MQ=255)
                                  caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                     <  1:215113/150‑1 (MQ=255)
                                  caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                     >  2:215113/1‑150 (MQ=255)
                                  caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGGTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                     <  2:1246796/150‑1 (MQ=255)
                                   aTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTgg                                                                                                                    <  2:1338251/150‑1 (MQ=255)
                                     cAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGa                                                                                                                   <  1:399081/149‑1 (MQ=255)
                                     cAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGa                                                                                                                   >  2:399081/1‑149 (MQ=255)
                                      aaCACGCCGATCATCGCCGACACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCCACCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGAGACGATCACGCAGCGTCTTCGGGGATTCCGTCCCCTGGaaa                                                                                                                 >  1:1050475/1‑150 (MQ=255)
                                         acGCCGATCATCGCCGAAACGCGCCCGCCCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCg                                                                                                              >  2:1231408/1‑150 (MQ=255)
                                           gCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCACTGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAg                                                                                                            >  1:916785/1‑150 (MQ=255)
                                           gCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGAGACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAg                                                                                                            <  2:1542051/150‑1 (MQ=255)
                                           cccGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAg                                                                                                            <  2:1059807/149‑1 (MQ=255)
                                            ccGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGCTACGATCCCGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGt                                                                                                           >  1:592413/1‑150 (MQ=255)
                                                 catcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCgg                                                                                                      <  1:1423932/150‑1 (MQ=255)
                                                  atcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                     <  2:403291/150‑1 (MQ=255)
                                                  atcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGGCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                     <  1:1073815/150‑1 (MQ=255)
                                                    cGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATATGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGAt                                                                                                   >  1:708632/1‑150 (MQ=255)
                                                     gCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATc                                                                                                  <  1:442476/150‑1 (MQ=255)
                                                     gCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATc                                                                                                  >  1:170099/1‑150 (MQ=255)
                                                        gAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATcacc                                                                                               <  2:307367/150‑1 (MQ=255)
                                                         aaaCGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATcacca                                                                                              >  1:1213641/1‑150 (MQ=255)
                                                               gcCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCa                                                                                        >  1:135666/1‑150 (MQ=255)
                                                                cccGACCAGCCCGGGCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCAc                                                                                       <  2:338240/150‑1 (MQ=255)
                                                                     ccAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCg                                                                                  >  1:1195464/1‑150 (MQ=255)
                                                                     ccAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCg                                                                                  >  2:1111368/1‑150 (MQ=255)
                                                                     ccAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCg                                                                                  <  2:1031223/150‑1 (MQ=255)
                                                                        gCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCTGGaa                                                                               >  2:1363034/1‑150 (MQ=255)
                                                                        gCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGaa                                                                               >  2:150878/1‑150 (MQ=255)
                                                                         cccGGTCAACGCCGCCAGACGGGCAAACCCGCTGGCATCCATCCGCACTTCGTCAATGCCGGTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGGTGCGGGATCACCACCGCCACTGCCGGAAg                                                                              <  1:798784/150‑1 (MQ=255)
                                                                                aaCGCCGCCAGACGGGCAAACCAGCTCTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGTGATCACCACCGCCACTGCCGGAAGCGGGttt                                                                       <  2:1139771/150‑1 (MQ=255)
                                                                                 aCGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGtttt                                                                      <  2:579006/150‑1 (MQ=255)
                                                                                  cgccgcCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttc                                                                     <  1:1090541/150‑1 (MQ=255)
                                                                                   gccgccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttct                                                                    <  2:372184/150‑1 (MQ=255)
                                                                                   gccgccAGACGGGAAAACCAGCTGTAATCCATCCGCACTTCGTCAATGCAGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttct                                                                    <  2:469357/150‑1 (MQ=255)
                                                                                    ccgccAGACGGGCAAACCAGCTGTCAACCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttctt                                                                   <  1:1237949/150‑1 (MQ=255)
                                                                                     cgccAGCCGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTAAATCTGCGTGATACGATCACGCAGCGTATTCAGTGCTTCCGTCCCCTGGAAATCGAGTTTCGGGATCTCCACCGCCACTGCCGGAAGCGGGTTttcttc                                                                  >  1:1582919/1‑150 (MQ=255)
                                                                                     cgccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttcttc                                                                  >  2:1187125/1‑150 (MQ=255)
                                                                                       ccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttct                                                                    >  2:799603/1‑146 (MQ=255)
                                                                                       ccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttct                                                                    <  1:799603/146‑1 (MQ=255)
                                                                                         cgACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCTCGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                              <  1:243611/149‑1 (MQ=255)
                                                                                         aGACGGGCAAACCAGCTGTCATCCATCCGCCCTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                              <  1:479033/150‑1 (MQ=255)
                                                                                         aGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGATAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                              <  1:238589/150‑1 (MQ=255)
                                                                                            cGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCata                                                           <  1:850302/150‑1 (MQ=255)
                                                                                             gggCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCatat                                                          <  1:938460/150‑1 (MQ=255)
                                                                                                cAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCa                                                       >  2:1431959/1‑150 (MQ=255)
                                                                                                 aaaCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAg                                                      <  2:511596/150‑1 (MQ=255)
                                                                                                 aaaCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTACTTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCTCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAg                                                      <  1:376826/150‑1 (MQ=255)
                                                                                                   aCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAgcc                                                    >  2:49498/1‑149 (MQ=255)
                                                                                                   aCCAGCAGTCATCCATCCGCACTTCGTCAAAGGCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGGCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAgcg                                                    <  1:716952/150‑1 (MQ=255)
                                                                                                     cAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGca                                                  <  1:1231408/150‑1 (MQ=255)
                                                                                                        cTGTCATCCATCCGCACTTCGTCATTGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcacca                                               <  2:170099/150‑1 (MQ=255)
                                                                                                         tGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                              >  1:1262735/1‑150 (MQ=255)
                                                                                                         tGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                              <  1:1114196/150‑1 (MQ=255)
                                                                                                         tGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                              >  1:1269501/1‑150 (MQ=255)
                                                                                                            catccatcCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCaa                                           >  2:1236919/1‑150 (MQ=255)
                                                                                                              tccatccTCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTcc                                                                 <  1:891841/126‑1 (MQ=255)
                                                                                                              tccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTcc                                                                 >  2:891841/1‑126 (MQ=255)
                                                                                                              tccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCaaac                                         >  2:1318658/1‑149 (MQ=255)
                                                                                                              tccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCaaaa                                         >  1:1619684/1‑150 (MQ=255)
                                                                                                                catccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAacc                                       >  1:48828/1‑150 (MQ=255)
                                                                                                                   ccGCACTTCGTCAATGCCGTTACTCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccaga                                    <  2:179356/150‑1 (MQ=255)
                                                                                                                     gCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATGCAGTGATGCCGTCCCCTGGAAATCGAGGTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagacc                                  <  2:706765/150‑1 (MQ=255)
                                                                                                                      cACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCTGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagacca                                 >  2:1636292/1‑150 (MQ=255)
                                                                                                                      cACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAcccagccca                                 >  1:1159502/1‑150 (MQ=255)
                                                                                                                              cAATGGCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGCAATTCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGa                         <  2:300334/150‑1 (MQ=255)
                                                                                                                                   ccGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCa                    >  1:278433/1‑150 (MQ=255)
                                                                                                                                           cTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGc            >  1:807790/1‑150 (MQ=255)
                                                                                                                                           cTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGAATATCCAGCGCACCACCAAAACCAGACCAGTTACGGACCTCACCCAGTGc            >  1:832798/1‑150 (MQ=255)
                                                                                                                                            tGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCg           >  1:1733105/1‑150 (MQ=255)
                                                                                                                                            tGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCg           <  2:1664731/150‑1 (MQ=255)
                                                                                                                                             gCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGt          >  1:1550261/1‑150 (MQ=255)
                                                                                                                                             gCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGTTCACCACCGCCACTGCCGGATGCGGGTTTTCTTCCAGCATATCCAGCGCGCCACCAAAACCAGCCCAGTTACGGAACTCACGCAGTGCGt          >  2:105538/1‑150 (MQ=255)
                                                                                                                                             gCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTAGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACACAAACCAGCCCAGTTACTGAACTCCCCCAGTGCgg          >  1:1389534/1‑149 (MQ=255)
                                                                                                                                               gTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCt        <  1:663344/150‑1 (MQ=255)
                                                                                                                                                tGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCtt       >  2:1115088/1‑150 (MQ=255)
                                                                                                                                                tGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCtt       >  1:577077/1‑150 (MQ=255)
                                                                                                                                                 gATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGGTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTATTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTc      <  2:73279/150‑1 (MQ=255)
                                                                                                                                                  aTACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCa     >  1:110253/1‑150 (MQ=255)
                                                                                                                                                    aCGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACg   <  2:728923/150‑1 (MQ=255)
                                                                                                                                                     cGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACga  >  1:411400/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACTGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGA  >  NC_000913/3601357‑3601655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: