Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,507 G→T 5.2% R194S (CGT→AGT) ‡ ftsX ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,5070GT 5.2% 299.0 / 5.0 97R194S (CGT→AGT) ‡ftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (47/45);  new base T (3/2);  total (50/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

TGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGAG  >  NC_000913/3601360‑3601656
                                                                                                                                                   |                                                                                                                                                     
tGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAt                                                                                                                                                     <  1:435033/150‑1 (MQ=255)
tGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAt                                                                                                                                                     <  1:683058/150‑1 (MQ=255)
tGTTAACGATGACGATGAACACGGCCGCCACCATCAACACTCCGAGCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAt                                                                                                                                                     <  2:894830/150‑1 (MQ=255)
  ttACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCa                                                                                                                                                   >  1:403291/1‑150 (MQ=255)
    aCCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACg                                                                                                                                                 >  1:1213893/1‑150 (MQ=255)
       gATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCACCGCCGCCAGACGGGCAAACCAGCTGTCATCCCTCCGCACTGCGGCAATGCCGTTAATCTGCGTGATACGATCACGCAg                                                                                                                                              >  1:73279/1‑150 (MQ=255)
       gATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATCGTATCACGCAg                                                                                                                                              <  1:1347452/150‑1 (MQ=255)
       gATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAg                                                                                                                                              >  2:938460/1‑150 (MQ=255)
        ttGACGAGGAACACGGCCGCCACCATCAACACGCCGATCAGCGCCGAAACGCGCCCGACCCGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGc                                                                                                                                             <  2:115674/149‑1 (MQ=255)
          gACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATCGTATCACGCAgatt                                                                                                                                           <  2:1632189/150‑4 (MQ=255)
             gAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCTCTTCGTCAATGCCGTTAATCTGCGTGCTACGATCACGCAGCGTAtt                                                                                                                                        >  1:1211450/1‑150 (MQ=255)
             gAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTAtt                                                                                                                                        >  1:307367/1‑150 (MQ=255)
               ggAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCa                                                                                                                                      >  2:801358/1‑150 (MQ=255)
               ggAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAGCACGCAGCGTATTCa                                                                                                                                      >  2:1002525/1‑150 (MQ=255)
                gAACACGGCCGCCTCCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAg                                                                                                                                     <  2:775908/150‑1 (MQ=255)
                     cGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCCCTTCGTCACTGCCGTTAATCTGCGTGATACGATCCCGCAGCGTAGTCAGTGCtt                                                                                                                                >  1:706765/1‑150 (MQ=255)
                     cGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGCTCAACGCCGCCAGACGGGCAAACCAGCTGTCAGCCATCCGCTCTTCCTCAATGCCGTTAATCTGCGTGCTACGATCACGCAGCGTATTCAGTGAtt                                                                                                                                >  1:451115/1‑150 (MQ=255)
                       gccgccACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCCTCCGCACTTCGTCAATGCCGTTACTCTGCGTGATACGCTCACGCAGCGGATTCAGTGATTcc                                                                                                                              >  1:300334/1‑150 (MQ=255)
                               caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                      >  2:215113/1‑150 (MQ=255)
                               caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                      <  1:215113/150‑1 (MQ=255)
                               caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGGTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                      <  2:1246796/150‑1 (MQ=255)
                                aTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTgg                                                                                                                     <  2:1338251/150‑1 (MQ=255)
                                  cAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGa                                                                                                                    <  1:399081/149‑1 (MQ=255)
                                  cAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGa                                                                                                                    >  2:399081/1‑149 (MQ=255)
                                   aaCACGCCGATCATCGCCGACACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCCACCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGAGACGATCACGCAGCGTCTTCGGGGATTCCGTCCCCTGGaaa                                                                                                                  >  1:1050475/1‑150 (MQ=255)
                                      acGCCGATCATCGCCGAAACGCGCCCGCCCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCg                                                                                                               >  2:1231408/1‑150 (MQ=255)
                                        gCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCACTGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAg                                                                                                             >  1:916785/1‑150 (MQ=255)
                                        gCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGAGACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAg                                                                                                             <  2:1542051/150‑1 (MQ=255)
                                        cccGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAg                                                                                                             <  2:1059807/149‑1 (MQ=255)
                                         ccGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGCTACGATCCCGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGt                                                                                                            >  1:592413/1‑150 (MQ=255)
                                              catcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCgg                                                                                                       <  1:1423932/150‑1 (MQ=255)
                                               atcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                      <  2:403291/150‑1 (MQ=255)
                                               atcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGGCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                      <  1:1073815/150‑1 (MQ=255)
                                                 cGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATATGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGAt                                                                                                    >  1:708632/1‑150 (MQ=255)
                                                  gCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATc                                                                                                   <  1:442476/150‑1 (MQ=255)
                                                  gCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATc                                                                                                   >  1:170099/1‑150 (MQ=255)
                                                     gAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATcacc                                                                                                <  2:307367/150‑1 (MQ=255)
                                                      aaaCGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATcacca                                                                                               >  1:1213641/1‑150 (MQ=255)
                                                            gcCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCa                                                                                         >  1:135666/1‑150 (MQ=255)
                                                             cccGACCAGCCCGGGCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCAc                                                                                        <  2:338240/150‑1 (MQ=255)
                                                                  ccAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCg                                                                                   >  1:1195464/1‑150 (MQ=255)
                                                                  ccAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCg                                                                                   >  2:1111368/1‑150 (MQ=255)
                                                                  ccAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCg                                                                                   <  2:1031223/150‑1 (MQ=255)
                                                                     gCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCTGGaa                                                                                >  2:1363034/1‑150 (MQ=255)
                                                                     gCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGaa                                                                                >  2:150878/1‑150 (MQ=255)
                                                                      cccGGTCAACGCCGCCAGACGGGCAAACCCGCTGGCATCCATCCGCACTTCGTCAATGCCGGTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGGTGCGGGATCACCACCGCCACTGCCGGAAg                                                                               <  1:798784/150‑1 (MQ=255)
                                                                             aaCGCCGCCAGACGGGCAAACCAGCTCTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGTGATCACCACCGCCACTGCCGGAAGCGGGttt                                                                        <  2:1139771/150‑1 (MQ=255)
                                                                              aCGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGtttt                                                                       <  2:579006/150‑1 (MQ=255)
                                                                               cgccgcCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttc                                                                      <  1:1090541/150‑1 (MQ=255)
                                                                                gccgccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttct                                                                     <  2:372184/150‑1 (MQ=255)
                                                                                gccgccAGACGGGAAAACCAGCTGTAATCCATCCGCACTTCGTCAATGCAGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttct                                                                     <  2:469357/150‑1 (MQ=255)
                                                                                 ccgccAGACGGGCAAACCAGCTGTCAACCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttctt                                                                    <  1:1237949/150‑1 (MQ=255)
                                                                                  cgccAGCCGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTAAATCTGCGTGATACGATCACGCAGCGTATTCAGTGCTTCCGTCCCCTGGAAATCGAGTTTCGGGATCTCCACCGCCACTGCCGGAAGCGGGTTttcttc                                                                   >  1:1582919/1‑150 (MQ=255)
                                                                                  cgccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttcttc                                                                   >  2:1187125/1‑150 (MQ=255)
                                                                                    ccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttct                                                                     <  1:799603/146‑1 (MQ=255)
                                                                                    ccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttct                                                                     >  2:799603/1‑146 (MQ=255)
                                                                                      cgACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCTCGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                               <  1:243611/149‑1 (MQ=255)
                                                                                      aGACGGGCAAACCAGCTGTCATCCATCCGCCCTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                               <  1:479033/150‑1 (MQ=255)
                                                                                      aGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGATAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                               <  1:238589/150‑1 (MQ=255)
                                                                                         cGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCata                                                            <  1:850302/150‑1 (MQ=255)
                                                                                          gggCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCatat                                                           <  1:938460/150‑1 (MQ=255)
                                                                                             cAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCa                                                        >  2:1431959/1‑150 (MQ=255)
                                                                                              aaaCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAg                                                       <  2:511596/150‑1 (MQ=255)
                                                                                              aaaCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTACTTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCTCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAg                                                       <  1:376826/150‑1 (MQ=255)
                                                                                                aCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAgcc                                                     >  2:49498/1‑149 (MQ=255)
                                                                                                aCCAGCAGTCATCCATCCGCACTTCGTCAAAGGCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGGCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAgcg                                                     <  1:716952/150‑1 (MQ=255)
                                                                                                  cAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGca                                                   <  1:1231408/150‑1 (MQ=255)
                                                                                                     cTGTCATCCATCCGCACTTCGTCATTGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcacca                                                <  2:170099/150‑1 (MQ=255)
                                                                                                      tGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                               <  1:1114196/150‑1 (MQ=255)
                                                                                                      tGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                               >  1:1269501/1‑150 (MQ=255)
                                                                                                      tGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                               >  1:1262735/1‑150 (MQ=255)
                                                                                                         catccatcCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCaa                                            >  2:1236919/1‑150 (MQ=255)
                                                                                                           tccatccTCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTcc                                                                  <  1:891841/126‑1 (MQ=255)
                                                                                                           tccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTcc                                                                  >  2:891841/1‑126 (MQ=255)
                                                                                                           tccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCaaac                                          >  2:1318658/1‑149 (MQ=255)
                                                                                                           tccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCaaaa                                          >  1:1619684/1‑150 (MQ=255)
                                                                                                             catccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAacc                                        >  1:48828/1‑150 (MQ=255)
                                                                                                                ccGCACTTCGTCAATGCCGTTACTCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccaga                                     <  2:179356/150‑1 (MQ=255)
                                                                                                                  gCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATGCAGTGATGCCGTCCCCTGGAAATCGAGGTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagacc                                   <  2:706765/150‑1 (MQ=255)
                                                                                                                   cACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCTGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagacca                                  >  2:1636292/1‑150 (MQ=255)
                                                                                                                   cACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAcccagccca                                  >  1:1159502/1‑150 (MQ=255)
                                                                                                                           cAATGGCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGCAATTCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGa                          <  2:300334/150‑1 (MQ=255)
                                                                                                                                ccGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCa                     >  1:278433/1‑150 (MQ=255)
                                                                                                                                        cTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGc             >  1:807790/1‑150 (MQ=255)
                                                                                                                                        cTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGAATATCCAGCGCACCACCAAAACCAGACCAGTTACGGACCTCACCCAGTGc             >  1:832798/1‑150 (MQ=255)
                                                                                                                                         tGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCg            >  1:1733105/1‑150 (MQ=255)
                                                                                                                                         tGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCg            <  2:1664731/150‑1 (MQ=255)
                                                                                                                                          gCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGt           >  1:1550261/1‑150 (MQ=255)
                                                                                                                                          gCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGTTCACCACCGCCACTGCCGGATGCGGGTTTTCTTCCAGCATATCCAGCGCGCCACCAAAACCAGCCCAGTTACGGAACTCACGCAGTGCGt           >  2:105538/1‑150 (MQ=255)
                                                                                                                                          gCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTAGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACACAAACCAGCCCAGTTACTGAACTCCCCCAGTGCgg           >  1:1389534/1‑149 (MQ=255)
                                                                                                                                            gTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCt         <  1:663344/150‑1 (MQ=255)
                                                                                                                                             tGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCtt        >  2:1115088/1‑150 (MQ=255)
                                                                                                                                             tGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCtt        >  1:577077/1‑150 (MQ=255)
                                                                                                                                              gATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGGTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTATTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTc       <  2:73279/150‑1 (MQ=255)
                                                                                                                                               aTACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCa      >  1:110253/1‑150 (MQ=255)
                                                                                                                                                 aCGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACg    <  2:728923/150‑1 (MQ=255)
                                                                                                                                                  cGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACga   >  1:411400/1‑150 (MQ=255)
                                                                                                                                                   gATCACGCAGCGCATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACgag  <  2:69455/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGAG  >  NC_000913/3601360‑3601656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: