Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,610,537 A→C 6.1% P399P (CCT→CCG yhhS ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,610,5370AC 6.1% 297.0 / 7.7 98P399P (CCT→CCGyhhSputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (40/52);  new base C (2/4);  total (42/56)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

CTTCCGTTATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGC  >  NC_000913/3610388‑3610678
                                                                                                                                                     |                                                                                                                                              
ctTCCGTTATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCa                                                                                                                                                >  1:907106/1‑150 (MQ=255)
  tCCGTTATTCTTGGTAACGTGGGTAATCCACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCTGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACTCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAgg                                                                                                                                              <  2:1119249/150‑1 (MQ=255)
  tCCGTTATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAgg                                                                                                                                              <  1:923480/150‑1 (MQ=255)
     gTTATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGAc                                                                                                                                           <  1:1110924/150‑1 (MQ=255)
         ttCTGGGTAACGTGGGTAATCAACTGCTGCCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCATCAGGGACgtgt                                                                                                                                       >  1:1293195/1‑150 (MQ=255)
            tGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                   >  2:1652752/1‑150 (MQ=255)
             gggTAACGTGGGTAATCAACTGCTGCCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                   >  2:1463662/1‑150 (MQ=255)
             gggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                   <  2:707211/150‑1 (MQ=255)
              ggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCgg                                                                                                                                  <  1:514098/150‑1 (MQ=255)
              ggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCAGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATGCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCgg                                                                                                                                  <  1:289669/150‑1 (MQ=255)
               gTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGCGGCGGCCTCAGGGACGTGTTCCGGa                                                                                                                                 >  1:154999/1‑150 (MQ=255)
               gTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGa                                                                                                                                 <  1:1672905/150‑1 (MQ=255)
                 aaCGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAgg                                                                                                                               <  2:165678/150‑1 (MQ=255)
                  aCGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGACCGCTGACGATCAGCAAAAAGCGCAGACCAACGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGc                                                                                                                              <  2:149398/150‑1 (MQ=255)
                   cGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGcc                                                                                                                             <  2:1155426/150‑1 (MQ=255)
                   cGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGcc                                                                                                                             <  2:1212377/150‑1 (MQ=255)
                      gggTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGATTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGCCgcc                                                                                                                          <  2:1252525/150‑3 (MQ=255)
                       ggTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttt                                                                                                                        <  1:1473912/150‑1 (MQ=255)
                        gTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttt                                                                                                                       <  1:322257/150‑1 (MQ=255)
                          aaTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCATCAAAAATCGCAGACCACCGCAGAAAACATCATTAATACGCTTGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttttt                                                                                                                     <  1:1510793/150‑1 (MQ=255)
                          aaTCAACTGCTGACCAACCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttttt                                                                                                                     <  1:150205/150‑1 (MQ=255)
                           aTCAACTTCTGTCCATGCAAATTACGCTGCCCGCTGACGATCTGCTAAAAGCGCAGACCACCGCAGAAAACATCAATAATACGCTGGTTCTTCAGTAAGTTTAAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                    <  2:1381750/150‑1 (MQ=255)
                           aTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACAGCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                    <  1:681/150‑1 (MQ=255)
                           aTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCCCAGACCGCCGCAGAAACCATCATTAATACGCTGGATATTGAGTAAGTGTTAAGATGATGATGCGGACTCAGTGACGAGTTCCGTAGTCCGgtttttt                                                                                                                    >  2:93418/1‑150 (MQ=255)
                             cAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTAAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTaa                                                                                                                  >  2:1102172/1‑150 (MQ=255)
                               aCTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATc                                                                                                                >  1:435897/1‑150 (MQ=255)
                               aCTGCTGACCATGCAAATTACGCTGCCCGCTGACGAACAAAAAAAACCGCTCCCCAACGCAGAAAAAATCATAAATACGGTGGTTATTCTTTAAGTTTTAAGATGATGAGCCGGCATCAGGGACGTGTTCCGAAGGCCGTTTTTTTAATc                                                                                                                >  2:1328524/1‑150 (MQ=255)
                                ctgctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCg                                                                                                               <  1:127945/150‑1 (MQ=255)
                                  gctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGcc                                                                                                             <  1:752667/150‑1 (MQ=255)
                                   ctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCa                                                                                                            <  1:991249/150‑1 (MQ=255)
                                   ctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCa                                                                                                            <  1:639200/150‑1 (MQ=255)
                                     gACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACg                                                                                                          <  2:1631463/150‑1 (MQ=255)
                                      aCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGt                                                                                                         >  2:1561000/1‑150 (MQ=255)
                                       ccATGCAAATTACGCGGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTCGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTc                                                                                                        <  1:1084689/150‑1 (MQ=255)
                                        cATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCa                                                                                                       >  2:1529623/1‑150 (MQ=255)
                                         aTGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAg                                                                                                      >  1:997797/1‑150 (MQ=255)
                                          tGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGt                                                                                                     >  1:584728/1‑150 (MQ=255)
                                            cAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGtaa                                                                                                   >  1:340072/1‑150 (MQ=255)
                                              aaTTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGtaata                                                                                                 <  1:1542657/150‑1 (MQ=255)
                                                atACGCTGCCCGCTGACGATCTGCAAACAGCGCAGACCACCTCAGACAACATAATTAATTCGCTGGTTATTCAGTAAGTCTTAACATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAc                                                                                               <  1:292060/149‑1 (MQ=255)
                                                 tACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTACTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAcg                                                                                              <  1:439213/150‑1 (MQ=255)
                                                  aCGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTTAATCGCCACGTCAGTAATAAcg                                                                                              >  2:710021/1‑150 (MQ=255)
                                                     cTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAt                                                                                          >  1:761023/1‑150 (MQ=255)
                                                     cTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAt                                                                                          <  1:222391/150‑1 (MQ=255)
                                                      tGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAtt                                                                                         >  1:428617/1‑150 (MQ=255)
                                                       gCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTg                                                                                        >  2:1325422/1‑150 (MQ=255)
                                                       gCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTg                                                                                        <  1:1346059/150‑1 (MQ=255)
                                                        cccGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGc                                                                                       <  2:1731293/150‑1 (MQ=255)
                                                         ccGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACCTCAGTAATAACGCGCTTGCg                                                                                      >  1:1037833/1‑150 (MQ=255)
                                                           gCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAc                                                                                    >  1:988383/1‑150 (MQ=255)
                                                            cTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAcc                                                                                   <  2:781167/150‑1 (MQ=255)
                                                              gACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAg                                                                                 <  2:55812/150‑1 (MQ=255)
                                                                cGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTc                                                                               >  2:967220/1‑150 (MQ=255)
                                                                 gATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTcc                                                                              <  1:700607/150‑1 (MQ=255)
                                                                 gATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTcc                                                                              >  2:477584/1‑150 (MQ=255)
                                                                  aTCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACAAGTccc                                                                             >  2:827723/1‑150 (MQ=255)
                                                                    cAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgc                                                                           >  1:533884/1‑150 (MQ=255)
                                                                     aGCAAATTGCGCAGACCCCCGCAGAATCCATCAGTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGTGGCGGGTTGTTTAATCGCCCCGTCAGTAATAACGCGATTGCGACCATGCccgcc                                                                          >  2:917117/1‑150 (MQ=255)
                                                                     aGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGACACGTCAGTAATAACGCGATTGCGACCAGTCccgcc                                                                          >  1:121672/1‑150 (MQ=255)
                                                                      gCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccg                                                                         >  1:754940/1‑150 (MQ=255)
                                                                      gCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccg                                                                         <  1:1118410/150‑1 (MQ=255)
                                                                       cAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccgc                                                                        <  2:1583047/150‑1 (MQ=255)
                                                                       cAAAAAGCGCAGAACACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccgc                                                                        <  2:1591605/150‑1 (MQ=255)
                                                                             gcgcAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAg                                                                  <  2:340072/150‑1 (MQ=255)
                                                                             ccgcAGTCCACCGCACAAAACATCATTTATAGGCTGGTTATTCCGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAg                                                                  <  2:1505162/149‑1 (MQ=255)
                                                                                   accaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGGGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAAt                                                            <  1:1652487/150‑1 (MQ=255)
                                                                                    ccaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATc                                                           >  1:1551145/1‑150 (MQ=255)
                                                                                    ccaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATc                                                           >  1:1692024/1‑150 (MQ=255)
                                                                                       ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                        >  2:1382040/1‑150 (MQ=255)
                                                                                       ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCAACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                        <  1:1118912/150‑1 (MQ=255)
                                                                                       ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCAGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                        >  2:1194779/1‑150 (MQ=255)
                                                                                        cGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCg                                                       >  2:927557/1‑150 (MQ=255)
                                                                                          cAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGt                                                     <  1:1529623/150‑1 (MQ=255)
                                                                                            gAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCTCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAc                                                   >  1:146054/1‑150 (MQ=255)
                                                                                            gAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGCAGCCCGTTTTTTTAATAGCCACGTCAGTAATAACGCGATTGCGACCAGTCGCGCCGCCGCCAGATAAATCACCGGTAc                                                   >  1:966636/1‑150 (MQ=255)
                                                                                               aaCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgc                                                 <  1:710021/150‑1 (MQ=255)
                                                                                               aaCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgcc                                                <  2:328532/150‑1 (MQ=255)
                                                                                                aCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgccc                                               <  1:1715563/150‑1 (MQ=255)
                                                                                                     atTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCa                                          <  2:971538/150‑1 (MQ=255)
                                                                                                        aaTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCt                                       <  2:614054/150‑1 (MQ=255)
                                                                                                        aaTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCt                                       <  2:420695/150‑1 (MQ=255)
                                                                                                               tGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAcc                                <  2:515655/150‑1 (MQ=255)
                                                                                                                ggTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCTGATAAATCACCGGTACGCCCGCCCAGCTCATCCcca                               >  1:386350/1‑150 (MQ=255)
                                                                                                                    aTTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAccagccc                           >  2:1692665/1‑150 (MQ=255)
                                                                                                                        aGTAAGTTTTTAGATGATGAGGCGGCCTCAGGGACGTGATCCGGAGGCCGTGTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCTGCTCATCACcagcccagcc                       <  1:204652/150‑1 (MQ=255)
                                                                                                                        aGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCCCGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCCCCGGTACGCCCGCCCAGCTCATCACccgccccgcc                       >  2:1621676/1‑150 (MQ=255)
                                                                                                                           taGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGt                    <  1:1652752/149‑1 (MQ=255)
                                                                                                                             gTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTgg                  <  1:1458062/150‑1 (MQ=255)
                                                                                                                             gTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTGCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTgg                  <  2:1355442/150‑1 (MQ=255)
                                                                                                                              ttttAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGt                 >  1:664998/1‑150 (MQ=255)
                                                                                                                               tttAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGGTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTc                <  2:1248449/150‑1 (MQ=255)
                                                                                                                                ttAAGATGATGTGGCGGCGTCAGGGACGTGTTCCGGAGGCAGTTTTTTAAATCGCCACGTGAGTAATAACGCGTTTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCAAGTGGTcc               <  2:1127967/150‑1 (MQ=255)
                                                                                                                                  aaGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAg             >  1:402729/1‑150 (MQ=255)
                                                                                                                                    gatgatGAGGCGGCCTCAGGGACGTGTTCGGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACTACCGGCCATCTCATCACAAGCCCAGCCAGGGGAGCAGTc           >  2:1051326/1‑150 (MQ=255)
                                                                                                                                       gatgaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACg        <  1:11585/150‑1 (MQ=255)
                                                                                                                                          gaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCa     <  1:597606/150‑1 (MQ=255)
                                                                                                                                          aaaacggcCTCCGGAACACGTCCCTGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCa     >  1:1724403/5‑150 (MQ=255)
                                                                                                                                           aGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGCCCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCGCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCCCGCCac    >  1:560142/1‑149 (MQ=255)
                                                                                                                                             acggcCTCCGGAACACGTCCCTGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGc  >  2:1718908/2‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
CTTCCGTTATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGC  >  NC_000913/3610388‑3610678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: