Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,610,540 G→A 6.2% V398V (GTC→GTT yhhS ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,610,5400GA 6.2% 295.8 / 6.0 96V398V (GTC→GTTyhhSputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (40/50);  new base A (2/4);  total (42/54)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

GTTATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCC  >  NC_000913/3610393‑3610687
                                                                                                                                                   |                                                                                                                                                    
gTTATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGAc                                                                                                                                                    <  1:1110924/150‑1 (MQ=255)
    ttCTGGGTAACGTGGGTAATCAACTGCTGCCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCATCAGGGACgtgt                                                                                                                                                >  1:1293195/1‑150 (MQ=255)
       tGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                            >  2:1652752/1‑150 (MQ=255)
        gggTAACGTGGGTAATCAACTGCTGCCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                            >  2:1463662/1‑150 (MQ=255)
        gggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                            <  2:707211/150‑1 (MQ=255)
         ggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCgg                                                                                                                                           <  1:514098/150‑1 (MQ=255)
         ggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCAGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATGCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCgg                                                                                                                                           <  1:289669/150‑1 (MQ=255)
          gTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGCGGCGGCCTCAGGGACGTGTTCCGGa                                                                                                                                          >  1:154999/1‑150 (MQ=255)
          gTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGa                                                                                                                                          <  1:1672905/150‑1 (MQ=255)
            aaCGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAgg                                                                                                                                        <  2:165678/150‑1 (MQ=255)
             aCGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGACCGCTGACGATCAGCAAAAAGCGCAGACCAACGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGc                                                                                                                                       <  2:149398/150‑1 (MQ=255)
              cGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGcc                                                                                                                                      <  2:1155426/150‑1 (MQ=255)
              cGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGcc                                                                                                                                      <  2:1212377/150‑1 (MQ=255)
                 gggTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGATTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGCCgcc                                                                                                                                   <  2:1252525/150‑3 (MQ=255)
                  ggTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttt                                                                                                                                 <  1:1473912/150‑1 (MQ=255)
                   gTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttt                                                                                                                                <  1:322257/150‑1 (MQ=255)
                     aaTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCATCAAAAATCGCAGACCACCGCAGAAAACATCATTAATACGCTTGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttttt                                                                                                                              <  1:1510793/150‑1 (MQ=255)
                     aaTCAACTGCTGACCAACCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttttt                                                                                                                              <  1:150205/150‑1 (MQ=255)
                      aTCAACTTCTGTCCATGCAAATTACGCTGCCCGCTGACGATCTGCTAAAAGCGCAGACCACCGCAGAAAACATCAATAATACGCTGGTTCTTCAGTAAGTTTAAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                             <  2:1381750/150‑1 (MQ=255)
                      aTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACAGCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                             <  1:681/150‑1 (MQ=255)
                      aTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCCCAGACCGCCGCAGAAACCATCATTAATACGCTGGATATTGAGTAAGTGTTAAGATGATGATGCGGACTCAGTGACGAGTTCCGTAGTCCGgtttttt                                                                                                                             >  2:93418/1‑150 (MQ=255)
                        cAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTAAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTaa                                                                                                                           >  2:1102172/1‑150 (MQ=255)
                          aCTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATc                                                                                                                         >  1:435897/1‑150 (MQ=255)
                          aCTGCTGACCATGCAAATTACGCTGCCCGCTGACGAACAAAAAAAACCGCTCCCCAACGCAGAAAAAATCATAAATACGGTGGTTATTCTTTAAGTTTTAAGATGATGAGCCGGCATCAGGGACGTGTTCCGAAGGCCGTTTTTTTAATc                                                                                                                         >  2:1328524/1‑150 (MQ=255)
                           ctgctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCg                                                                                                                        <  1:127945/150‑1 (MQ=255)
                             gctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGcc                                                                                                                      <  1:752667/150‑1 (MQ=255)
                              ctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCa                                                                                                                     <  1:639200/150‑1 (MQ=255)
                              ctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCa                                                                                                                     <  1:991249/150‑1 (MQ=255)
                                gACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACg                                                                                                                   <  2:1631463/150‑1 (MQ=255)
                                 aCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGt                                                                                                                  >  2:1561000/1‑150 (MQ=255)
                                  ccATGCAAATTACGCGGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTCGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTc                                                                                                                 <  1:1084689/150‑1 (MQ=255)
                                   cATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCa                                                                                                                >  2:1529623/1‑150 (MQ=255)
                                    aTGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAg                                                                                                               >  1:997797/1‑150 (MQ=255)
                                     tGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGt                                                                                                              >  1:584728/1‑150 (MQ=255)
                                       cAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGtaa                                                                                                            >  1:340072/1‑150 (MQ=255)
                                         aaTTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGtaata                                                                                                          <  1:1542657/150‑1 (MQ=255)
                                           atACGCTGCCCGCTGACGATCTGCAAACAGCGCAGACCACCTCAGACAACATAATTAATTCGCTGGTTATTCAGTAAGTCTTAACATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAc                                                                                                        <  1:292060/149‑1 (MQ=255)
                                            tACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTACTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAcg                                                                                                       <  1:439213/150‑1 (MQ=255)
                                             aCGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTTAATCGCCACGTCAGTAATAAcg                                                                                                       >  2:710021/1‑150 (MQ=255)
                                                cTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAt                                                                                                   <  1:222391/150‑1 (MQ=255)
                                                cTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAt                                                                                                   >  1:761023/1‑150 (MQ=255)
                                                 tGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAtt                                                                                                  >  1:428617/1‑150 (MQ=255)
                                                  gCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTg                                                                                                 >  2:1325422/1‑150 (MQ=255)
                                                  gCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTg                                                                                                 <  1:1346059/150‑1 (MQ=255)
                                                   cccGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGc                                                                                                <  2:1731293/150‑1 (MQ=255)
                                                    ccGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACCTCAGTAATAACGCGCTTGCg                                                                                               >  1:1037833/1‑150 (MQ=255)
                                                      gCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAc                                                                                             >  1:988383/1‑150 (MQ=255)
                                                       cTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAcc                                                                                            <  2:781167/150‑1 (MQ=255)
                                                         gACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAg                                                                                          <  2:55812/150‑1 (MQ=255)
                                                           cGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTc                                                                                        >  2:967220/1‑150 (MQ=255)
                                                            gATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTcc                                                                                       <  1:700607/150‑1 (MQ=255)
                                                            gATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTcc                                                                                       >  2:477584/1‑150 (MQ=255)
                                                             aTCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACAAGTccc                                                                                      >  2:827723/1‑150 (MQ=255)
                                                               cAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgc                                                                                    >  1:533884/1‑150 (MQ=255)
                                                                aGCAAATTGCGCAGACCCCCGCAGAATCCATCAGTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGTGGCGGGTTGTTTAATCGCCCCGTCAGTAATAACGCGATTGCGACCATGCccgcc                                                                                   >  2:917117/1‑150 (MQ=255)
                                                                aGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGACACGTCAGTAATAACGCGATTGCGACCAGTCccgcc                                                                                   >  1:121672/1‑150 (MQ=255)
                                                                 gCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccg                                                                                  >  1:754940/1‑150 (MQ=255)
                                                                 gCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccg                                                                                  <  1:1118410/150‑1 (MQ=255)
                                                                  cAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccgc                                                                                 <  2:1583047/150‑1 (MQ=255)
                                                                  cAAAAAGCGCAGAACACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccgc                                                                                 <  2:1591605/150‑1 (MQ=255)
                                                                        gcgcAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAg                                                                           <  2:340072/150‑1 (MQ=255)
                                                                        ccgcAGTCCACCGCACAAAACATCATTTATAGGCTGGTTATTCCGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAg                                                                           <  2:1505162/149‑1 (MQ=255)
                                                                              accaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGGGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAAt                                                                     <  1:1652487/150‑1 (MQ=255)
                                                                               ccaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATc                                                                    >  1:1692024/1‑150 (MQ=255)
                                                                               ccaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATc                                                                    >  1:1551145/1‑150 (MQ=255)
                                                                                  ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                                 >  2:1382040/1‑150 (MQ=255)
                                                                                  ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCAACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                                 <  1:1118912/150‑1 (MQ=255)
                                                                                  ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCAGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                                 >  2:1194779/1‑150 (MQ=255)
                                                                                   cGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCg                                                                >  2:927557/1‑150 (MQ=255)
                                                                                     cAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGt                                                              <  1:1529623/150‑1 (MQ=255)
                                                                                       gAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCTCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAc                                                            >  1:146054/1‑150 (MQ=255)
                                                                                       gAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGCAGCCCGTTTTTTTAATAGCCACGTCAGTAATAACGCGATTGCGACCAGTCGCGCCGCCGCCAGATAAATCACCGGTAc                                                            >  1:966636/1‑150 (MQ=255)
                                                                                          aaCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgc                                                          <  1:710021/150‑1 (MQ=255)
                                                                                          aaCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgcc                                                         <  2:328532/150‑1 (MQ=255)
                                                                                           aCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgccc                                                        <  1:1715563/150‑1 (MQ=255)
                                                                                                atTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCa                                                   <  2:971538/150‑1 (MQ=255)
                                                                                                   aaTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCt                                                <  2:420695/150‑1 (MQ=255)
                                                                                                   aaTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCt                                                <  2:614054/150‑1 (MQ=255)
                                                                                                          tGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAcc                                         <  2:515655/150‑1 (MQ=255)
                                                                                                           ggTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCTGATAAATCACCGGTACGCCCGCCCAGCTCATCCcca                                        >  1:386350/1‑150 (MQ=255)
                                                                                                               aTTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAccagccc                                    >  2:1692665/1‑150 (MQ=255)
                                                                                                                   aGTAAGTTTTTAGATGATGAGGCGGCCTCAGGGACGTGATCCGGAGGCCGTGTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCTGCTCATCACcagcccagcc                                <  1:204652/150‑1 (MQ=255)
                                                                                                                   aGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCCCGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCCCCGGTACGCCCGCCCAGCTCATCACccgccccgcc                                >  2:1621676/1‑150 (MQ=255)
                                                                                                                      taGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGt                             <  1:1652752/149‑1 (MQ=255)
                                                                                                                        gTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTgg                           <  1:1458062/150‑1 (MQ=255)
                                                                                                                        gTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTGCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTgg                           <  2:1355442/150‑1 (MQ=255)
                                                                                                                         ttttAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGt                          >  1:664998/1‑150 (MQ=255)
                                                                                                                          tttAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGGTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTc                         <  2:1248449/150‑1 (MQ=255)
                                                                                                                           ttAAGATGATGTGGCGGCGTCAGGGACGTGTTCCGGAGGCAGTTTTTTAAATCGCCACGTGAGTAATAACGCGTTTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCAAGTGGTcc                        <  2:1127967/150‑1 (MQ=255)
                                                                                                                             aaGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAg                      >  1:402729/1‑150 (MQ=255)
                                                                                                                               gatgatGAGGCGGCCTCAGGGACGTGTTCGGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACTACCGGCCATCTCATCACAAGCCCAGCCAGGGGAGCAGTc                    >  2:1051326/1‑150 (MQ=255)
                                                                                                                                  gatgaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACg                 <  1:11585/150‑1 (MQ=255)
                                                                                                                                     gaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCa              <  1:597606/150‑1 (MQ=255)
                                                                                                                                     aaaacggcCTCCGGAACACGTCCCTGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCa              >  1:1724403/5‑150 (MQ=255)
                                                                                                                                      aGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGCCCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCGCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCCCGCCac             >  1:560142/1‑149 (MQ=255)
                                                                                                                                        acggcCTCCGGAACACGTCCCTGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGc           >  2:1718908/2‑150 (MQ=255)
                                                                                                                                                 gggACGTGCTCCGGAGGCCGTTTTTTTCATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATcc  <  1:333680/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTTATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCC  >  NC_000913/3610393‑3610687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: