Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,610,544 T→C 6.4% H397R (CAC→CGC) ‡ yhhS ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,610,5440TC 6.4% 271.0 / 7.4 96H397R (CAC→CGC) ‡yhhSputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (38/50);  new base C (2/4);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

TTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCCATAA  >  NC_000913/3610397‑3610691
                                                                                                                                                   |                                                                                                                                                    
ttCTGGGTAACGTGGGTAATCAACTGCTGCCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCATCAGGGACgtgt                                                                                                                                                    >  1:1293195/1‑150 (MQ=255)
   tGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                                >  2:1652752/1‑150 (MQ=255)
    gggTAACGTGGGTAATCAACTGCTGCCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                                >  2:1463662/1‑150 (MQ=255)
    gggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCg                                                                                                                                                <  2:707211/150‑1 (MQ=255)
     ggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCgg                                                                                                                                               <  1:514098/150‑1 (MQ=255)
     ggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCAGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATGCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCgg                                                                                                                                               <  1:289669/150‑1 (MQ=255)
      gTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGCGGCGGCCTCAGGGACGTGTTCCGGa                                                                                                                                              >  1:154999/1‑150 (MQ=255)
      gTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGa                                                                                                                                              <  1:1672905/150‑1 (MQ=255)
        aaCGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAgg                                                                                                                                            <  2:165678/150‑1 (MQ=255)
         aCGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGACCGCTGACGATCAGCAAAAAGCGCAGACCAACGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGc                                                                                                                                           <  2:149398/150‑1 (MQ=255)
          cGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGcc                                                                                                                                          <  2:1155426/150‑1 (MQ=255)
          cGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGcc                                                                                                                                          <  2:1212377/150‑1 (MQ=255)
             gggTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGATTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGCCgcc                                                                                                                                       <  2:1252525/150‑3 (MQ=255)
              ggTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttt                                                                                                                                     <  1:1473912/150‑1 (MQ=255)
               gTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttt                                                                                                                                    <  1:322257/150‑1 (MQ=255)
                 aaTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCATCAAAAATCGCAGACCACCGCAGAAAACATCATTAATACGCTTGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttttt                                                                                                                                  <  1:1510793/150‑1 (MQ=255)
                 aaTCAACTGCTGACCAACCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttttt                                                                                                                                  <  1:150205/150‑1 (MQ=255)
                  aTCAACTTCTGTCCATGCAAATTACGCTGCCCGCTGACGATCTGCTAAAAGCGCAGACCACCGCAGAAAACATCAATAATACGCTGGTTCTTCAGTAAGTTTAAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                                 <  2:1381750/150‑1 (MQ=255)
                  aTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACAGCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                                 <  1:681/150‑1 (MQ=255)
                  aTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCCCAGACCGCCGCAGAAACCATCATTAATACGCTGGATATTGAGTAAGTGTTAAGATGATGATGCGGACTCAGTGACGAGTTCCGTAGTCCGgtttttt                                                                                                                                 >  2:93418/1‑150 (MQ=255)
                    cAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTAAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTaa                                                                                                                               >  2:1102172/1‑150 (MQ=255)
                      aCTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATc                                                                                                                             >  1:435897/1‑150 (MQ=255)
                      aCTGCTGACCATGCAAATTACGCTGCCCGCTGACGAACAAAAAAAACCGCTCCCCAACGCAGAAAAAATCATAAATACGGTGGTTATTCTTTAAGTTTTAAGATGATGAGCCGGCATCAGGGACGTGTTCCGAAGGCCGTTTTTTTAATc                                                                                                                             >  2:1328524/1‑150 (MQ=255)
                       ctgctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCg                                                                                                                            <  1:127945/150‑1 (MQ=255)
                         gctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGcc                                                                                                                          <  1:752667/150‑1 (MQ=255)
                          ctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCa                                                                                                                         <  1:991249/150‑1 (MQ=255)
                          ctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCa                                                                                                                         <  1:639200/150‑1 (MQ=255)
                            gACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACg                                                                                                                       <  2:1631463/150‑1 (MQ=255)
                             aCCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGt                                                                                                                      >  2:1561000/1‑150 (MQ=255)
                              ccATGCAAATTACGCGGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTCGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTc                                                                                                                     <  1:1084689/150‑1 (MQ=255)
                               cATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCa                                                                                                                    >  2:1529623/1‑150 (MQ=255)
                                aTGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAg                                                                                                                   >  1:997797/1‑150 (MQ=255)
                                 tGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGt                                                                                                                  >  1:584728/1‑150 (MQ=255)
                                   cAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGtaa                                                                                                                >  1:340072/1‑150 (MQ=255)
                                     aaTTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGtaata                                                                                                              <  1:1542657/150‑1 (MQ=255)
                                       atACGCTGCCCGCTGACGATCTGCAAACAGCGCAGACCACCTCAGACAACATAATTAATTCGCTGGTTATTCAGTAAGTCTTAACATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAc                                                                                                            <  1:292060/149‑1 (MQ=255)
                                        tACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTACTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAcg                                                                                                           <  1:439213/150‑1 (MQ=255)
                                         aCGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTTAATCGCCACGTCAGTAATAAcg                                                                                                           >  2:710021/1‑150 (MQ=255)
                                            cTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAt                                                                                                       >  1:761023/1‑150 (MQ=255)
                                            cTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAt                                                                                                       <  1:222391/150‑1 (MQ=255)
                                             tGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAtt                                                                                                      >  1:428617/1‑150 (MQ=255)
                                              gCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTg                                                                                                     <  1:1346059/150‑1 (MQ=255)
                                              gCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTg                                                                                                     >  2:1325422/1‑150 (MQ=255)
                                               cccGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGc                                                                                                    <  2:1731293/150‑1 (MQ=255)
                                                ccGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACCTCAGTAATAACGCGCTTGCg                                                                                                   >  1:1037833/1‑150 (MQ=255)
                                                  gCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAc                                                                                                 >  1:988383/1‑150 (MQ=255)
                                                   cTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAcc                                                                                                <  2:781167/150‑1 (MQ=255)
                                                     gACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAg                                                                                              <  2:55812/150‑1 (MQ=255)
                                                       cGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTc                                                                                            >  2:967220/1‑150 (MQ=255)
                                                        gATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTcc                                                                                           <  1:700607/150‑1 (MQ=255)
                                                        gATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTcc                                                                                           >  2:477584/1‑150 (MQ=255)
                                                         aTCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACAAGTccc                                                                                          >  2:827723/1‑150 (MQ=255)
                                                           cAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgc                                                                                        >  1:533884/1‑150 (MQ=255)
                                                            aGCAAATTGCGCAGACCCCCGCAGAATCCATCAGTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGTGGCGGGTTGTTTAATCGCCCCGTCAGTAATAACGCGATTGCGACCATGCccgcc                                                                                       >  2:917117/1‑150 (MQ=255)
                                                            aGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGACACGTCAGTAATAACGCGATTGCGACCAGTCccgcc                                                                                       >  1:121672/1‑150 (MQ=255)
                                                             gCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccg                                                                                      >  1:754940/1‑150 (MQ=255)
                                                             gCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccg                                                                                      <  1:1118410/150‑1 (MQ=255)
                                                              cAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccgc                                                                                     <  2:1583047/150‑1 (MQ=255)
                                                              cAAAAAGCGCAGAACACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccgc                                                                                     <  2:1591605/150‑1 (MQ=255)
                                                                    gcgcAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAg                                                                               <  2:340072/150‑1 (MQ=255)
                                                                    ccgcAGTCCACCGCACAAAACATCATTTATAGGCTGGTTATTCCGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAg                                                                               <  2:1505162/149‑1 (MQ=255)
                                                                          accaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGGGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAAt                                                                         <  1:1652487/150‑1 (MQ=255)
                                                                           ccaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATc                                                                        >  1:1692024/1‑150 (MQ=255)
                                                                           ccaccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATc                                                                        >  1:1551145/1‑150 (MQ=255)
                                                                              ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                                     >  2:1382040/1‑150 (MQ=255)
                                                                              ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCAACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                                     <  1:1118912/150‑1 (MQ=255)
                                                                              ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCAGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                                     >  2:1194779/1‑150 (MQ=255)
                                                                               cGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCg                                                                    >  2:927557/1‑150 (MQ=255)
                                                                                 cAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGt                                                                  <  1:1529623/150‑1 (MQ=255)
                                                                                   gAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCTCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAc                                                                >  1:146054/1‑150 (MQ=255)
                                                                                   gAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGCAGCCCGTTTTTTTAATAGCCACGTCAGTAATAACGCGATTGCGACCAGTCGCGCCGCCGCCAGATAAATCACCGGTAc                                                                >  1:966636/1‑150 (MQ=255)
                                                                                      aaCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgc                                                              <  1:710021/150‑1 (MQ=255)
                                                                                      aaCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgcc                                                             <  2:328532/150‑1 (MQ=255)
                                                                                       aCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgccc                                                            <  1:1715563/150‑1 (MQ=255)
                                                                                            atTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCa                                                       <  2:971538/150‑1 (MQ=255)
                                                                                               aaTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCt                                                    <  2:614054/150‑1 (MQ=255)
                                                                                               aaTACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCt                                                    <  2:420695/150‑1 (MQ=255)
                                                                                                      tGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAcc                                             <  2:515655/150‑1 (MQ=255)
                                                                                                       ggTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCTGATAAATCACCGGTACGCCCGCCCAGCTCATCCcca                                            >  1:386350/1‑150 (MQ=255)
                                                                                                           aTTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAccagccc                                        >  2:1692665/1‑150 (MQ=255)
                                                                                                               aGTAAGTTTTTAGATGATGAGGCGGCCTCAGGGACGTGATCCGGAGGCCGTGTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCTGCTCATCACcagcccagcc                                    <  1:204652/150‑1 (MQ=255)
                                                                                                               aGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCCCGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCCCCGGTACGCCCGCCCAGCTCATCACccgccccgcc                                    >  2:1621676/1‑150 (MQ=255)
                                                                                                                  taGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGt                                 <  1:1652752/149‑1 (MQ=255)
                                                                                                                    gTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTgg                               <  1:1458062/150‑1 (MQ=255)
                                                                                                                    gTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTGCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTgg                               <  2:1355442/150‑1 (MQ=255)
                                                                                                                     ttttAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGt                              >  1:664998/1‑150 (MQ=255)
                                                                                                                      tttAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGGTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTc                             <  2:1248449/150‑1 (MQ=255)
                                                                                                                       ttAAGATGATGTGGCGGCGTCAGGGACGTGTTCCGGAGGCAGTTTTTTAAATCGCCACGTGAGTAATAACGCGTTTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCAAGTGGTcc                            <  2:1127967/150‑1 (MQ=255)
                                                                                                                         aaGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAg                          >  1:402729/1‑150 (MQ=255)
                                                                                                                           gatgatGAGGCGGCCTCAGGGACGTGTTCGGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACTACCGGCCATCTCATCACAAGCCCAGCCAGGGGAGCAGTc                        >  2:1051326/1‑150 (MQ=255)
                                                                                                                              gatgaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACg                     <  1:11585/150‑1 (MQ=255)
                                                                                                                                 gaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCa                  <  1:597606/150‑1 (MQ=255)
                                                                                                                                 aaaacggcCTCCGGAACACGTCCCTGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCa                  >  1:1724403/5‑150 (MQ=255)
                                                                                                                                  aGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGCCCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCGCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCCCGCCac                 >  1:560142/1‑149 (MQ=255)
                                                                                                                                    acggcCTCCGGAACACGTCCCTGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGc               >  2:1718908/2‑150 (MQ=255)
                                                                                                                                             gggACGTGCTCCGGAGGCCGTTTTTTTCATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATcc      <  1:333680/150‑1 (MQ=255)
                                                                                                                                                 cGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCCATaa  <  2:716180/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCCATAA  >  NC_000913/3610397‑3610691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: