Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,616,399 T→G 8.7% V73G (GTG→GGG)  nikC → nickel ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,616,3990TG 8.7% 245.4 / 8.9 103V73G (GTG→GGG) nikCnickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (49/44);  new base G (2/8);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 9.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

ATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATT  >  NC_000913/3616251‑3616544
                                                                                                                                                    |                                                                                                                                                 
aTTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCgtg                                                                                                                                                  <  1:347187/150‑1 (MQ=255)
 ttGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACTGGCGATTGATTGGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACACGCgggt                                                                                                                                                 <  1:263636/150‑1 (MQ=255)
  tGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTc                                                                                                                                                <  2:466581/150‑1 (MQ=255)
     gcTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCt                                                                                                                                             <  1:1144663/150‑1 (MQ=255)
      cTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtc                                                                                                                                            >  1:474872/1‑150 (MQ=255)
       tCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcg                                                                                                                                           >  2:723094/1‑150 (MQ=255)
        ctccagccagTGGTGGCTGCCGTAGGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGCTGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACACGCGGGTCGCtgcc                                                                                                                                          <  1:305321/148‑4 (MQ=255)
        caggagcgagTGGTGGCTGCCGGATGAGCCACAGGCGATTGATTTGCCGTCGCGGCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCAGATGGCAGCGACCCGCGTGTCGCtcgg                                                                                                                                          <  2:881961/146‑1 (MQ=255)
        cacctcccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcgg                                                                                                                                          <  1:1050078/144‑1 (MQ=255)
        cACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcgg                                                                                                                                          <  1:488345/150‑1 (MQ=255)
        cACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcgg                                                                                                                                          <  2:1334966/150‑1 (MQ=255)
        cACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcgg                                                                                                                                          >  2:1531004/1‑150 (MQ=255)
        cACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACACGCGGGTCGCtgcc                                                                                                                                          <  1:802784/150‑4 (MQ=255)
         aCCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACACGCGGGTCGCtgcca                                                                                                                                         <  1:1173878/150‑5 (MQ=255)
         aCCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGGCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACACGCGGGTCGCtgcca                                                                                                                                         <  1:883381/150‑5 (MQ=255)
              ccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttcggt                                                                                                                                    <  1:1062354/150‑1 (MQ=255)
              ccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttcggt                                                                                                                                    <  2:744403/150‑1 (MQ=255)
              ccagGGGTGGCTGCCGTAGGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttcggt                                                                                                                                    <  1:1325525/150‑1 (MQ=255)
               cagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTa                                                                                                                                   <  1:1674/150‑1 (MQ=255)
                  tggtggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGGTCGGGTCGGTAATg                                                                                                                                >  2:1519772/1‑150 (MQ=255)
                    gtggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGgc                                                                                                                              >  2:1580148/1‑150 (MQ=255)
                      ggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGCCCCGCGTGTCGCTCGGTTCGGTAATGgccg                                                                                                                            >  2:455421/1‑149 (MQ=255)
                            cGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGGCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCctgcctgc                                                                                                                      <  1:163643/150‑1 (MQ=255)
                             gTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGCTCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCctgct                                                                                                                     >  1:568252/1‑150 (MQ=255)
                             gTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCctgct                                                                                                                     >  2:868325/1‑150 (MQ=255)
                                  ccccACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTgg                                                                                                                <  1:1444534/149‑1 (MQ=255)
                                  aCCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTgg                                                                                                                >  2:107530/1‑150 (MQ=255)
                                   cccACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGt                                                                                                               <  2:1257035/150‑1 (MQ=255)
                                       caGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTg                                                                                                           <  1:1006895/150‑1 (MQ=255)
                                         ggCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATATTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGAc                                                                                                         >  2:570060/1‑150 (MQ=255)
                                            gattgattTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACAtt                                                                                                      <  2:1119756/150‑1 (MQ=255)
                                             attgattTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACTTTa                                                                                                     <  1:976093/150‑1 (MQ=255)
                                             agtgattTGCCGGCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGGCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTa                                                                                                     <  1:1277820/148‑1 (MQ=255)
                                              ttgattTGCCGTCGCGCCTGATTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAg                                                                                                    <  2:231855/150‑1 (MQ=255)
                                                 attTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGc                                                                                                 <  2:825662/150‑1 (MQ=255)
                                                     gCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCCGACATTAGGGCTGGt                                                                                             <  2:1043033/150‑1 (MQ=255)
                                                       cGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCGGGttt                                                                                           >  1:537754/1‑149 (MQ=255)
                                                        gTCGCGCCTGGTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCGGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCAGCTGGTGCGGACATTAGGGCTGGTTAt                                                                                          <  1:1377609/150‑1 (MQ=255)
                                                           gcgcCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCTATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTgg                                                                                       <  2:929315/150‑1 (MQ=255)
                                                            cgcCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTggc                                                                                      >  2:1475187/1‑150 (MQ=255)
                                                            cgcCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTggc                                                                                      >  2:1468319/1‑150 (MQ=255)
                                                            cgcCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTGATTggc                                                                                      >  2:1294697/1‑150 (MQ=255)
                                                               cTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTggcggc                                                                                   >  2:1252212/1‑150 (MQ=255)
                                                                  cTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAgc                                                                                >  1:916990/1‑150 (MQ=255)
                                                                   tttCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAgcg                                                                               >  1:1139360/1‑150 (MQ=255)
                                                                      cGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGGTGACATTAGGGGTGGTTATTGGCGGCAGCGCCg                                                                            >  2:786513/1‑150 (MQ=255)
                                                                       gCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCgg                                                                           <  2:474872/150‑1 (MQ=255)
                                                                        ccGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGGCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCggg                                                                          <  1:569752/150‑1 (MQ=255)
                                                                         cGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGt                                                                         <  1:1488403/150‑1 (MQ=255)
                                                                            aTGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCGGGTTATTGGCGGCAGCGCCGGGTTGa                                                                      >  1:873505/1‑150 (MQ=255)
                                                                              gcgcAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCTGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGAtt                                                                    <  1:736308/150‑1 (MQ=255)
                                                                              gcgcAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGAtt                                                                    <  2:1732371/150‑1 (MQ=255)
                                                                               cgcAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTg                                                                   >  1:1141058/1‑150 (MQ=255)
                                                                               cgcAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTg                                                                   >  1:397110/1‑150 (MQ=255)
                                                                                gcagcaCTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTgg                                                                  >  1:691483/1‑150 (MQ=255)
                                                                                 cagcaCTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTggc                                                                 >  2:777955/1‑150 (MQ=255)
                                                                                  agcaCTGGCGGGGCACCGATCACTTAGGTCGCGACATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTggcg                                                                <  1:699763/150‑1 (MQ=255)
                                                                                   gcaCTGGCTGGGCACCGATCACTAAGGTCGCCATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGCTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGGTATTGGCGGCAGCGCCGGGTTGATTggcgg                                                               >  2:1351077/1‑150 (MQ=255)
                                                                                      ctggctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTggcgggcg                                                            >  1:921825/1‑150 (MQ=255)
                                                                                      ctggctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCGCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTggcgggcg                                                            >  1:1095986/1‑150 (MQ=255)
                                                                                      ctggctggGCACCGATCACTTAGGGCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTggcgggcg                                                            <  1:585791/150‑1 (MQ=255)
                                                                                        ggctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGcgcg                                                          >  2:348665/1‑150 (MQ=255)
                                                                                        ggctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGATGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGCGTTGATTGGCGGGcgcg                                                          >  2:893379/1‑150 (MQ=255)
                                                                                         gctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGt                                                         <  1:509025/150‑1 (MQ=255)
                                                                                          ctggGCACCGATCACTTCTGTCGCGTTATATGCTCGCGGCTGATGGCAGCGACCCGCGTGGCGCTCGGCTCGGCAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTTGCGGCAGCGCCGGGTTGATTGGCGGGCGCGtt                                                        <  1:809452/150‑1 (MQ=255)
                                                                                           tggGCACCGATGACTTAGCTCGCGATATTTTCTGGCGGCTGACGGCAGCGACCCGCGTGGCGCTCGGTTCGGTAATGGCCGGCCTGGTGGTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTg                                                       <  1:1377042/150‑1 (MQ=255)
                                                                                           tggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTg                                                       >  2:321203/1‑150 (MQ=255)
                                                                                           ggggCACCGAGCACGTCGGTCGCGATCTTTTCTCGCGGCGGATGGCAGCGACCCGCGGGGCGCGCGGTGCGGTAATGGCCGGCCTGCTGCTGGTGCGGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTg                                                       <  1:257183/149‑1 (MQ=255)
                                                                                             ggCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGAt                                                     >  1:159143/1‑150 (MQ=255)
                                                                                              gCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATc                                                    >  2:448886/1‑150 (MQ=255)
                                                                                              gCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCAGCCTGCTGCTGGTGCTGACATAACGGCTGGTTATTCGAGGCAGCGCCGGGTTGAATGGCGGGCGCGTAGATc                                                    >  1:207777/1‑150 (MQ=255)
                                                                                              gCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCGGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCGGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATc                                                    <  1:1129730/150‑1 (MQ=255)
                                                                                               cACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCTGGTTGATTGGCGGGCGCGTTGATCa                                                   >  2:348764/1‑150 (MQ=255)
                                                                                               cACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCa                                                   >  2:216016/1‑150 (MQ=255)
                                                                                               cACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCa                                                   >  2:1712747/1‑150 (MQ=255)
                                                                                                aCCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGGCGCTCGGTGCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAg                                                  <  1:486258/150‑1 (MQ=255)
                                                                                                   gATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGcc                                               >  2:1179409/1‑150 (MQ=255)
                                                                                                     tCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGccac                                             <  1:723094/150‑1 (MQ=255)
                                                                                                      cACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGccacc                                            >  1:1571115/1‑150 (MQ=255)
                                                                                                       aCTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGGGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGccacca                                           >  2:1631586/1‑150 (MQ=255)
                                                                                                        cTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCAt                                          >  2:1433575/1‑150 (MQ=255)
                                                                                                               cgcgATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTAGATTGGCGGGCGCGTTGATCAGGACACCATGCGCGTc                                   >  2:501485/1‑150 (MQ=255)
                                                                                                                  gATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGcc                                >  1:513379/1‑150 (MQ=255)
                                                                                                                     atTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCTGCAGCTCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGat                             >  1:1077704/1‑150 (MQ=255)
                                                                                                                     atTTTCTCGCGGCGGATGGCAGCGACCCGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGat                             <  2:1641633/150‑1 (MQ=255)
                                                                                                                         tctcGCGGCTGATGGCAGCGACCCGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGt                         <  1:868325/150‑1 (MQ=255)
                                                                                                                          ctcGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGtt                        >  1:934088/1‑150 (MQ=255)
                                                                                                                              cgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATg                    >  1:753086/1‑150 (MQ=255)
                                                                                                                                gCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGCCATTAGGGCTGGTTATTGGCGGTAGCGCCGGGTGGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGAc                  >  1:755802/1‑150 (MQ=255)
                                                                                                                                  ggATGGCAGCGTCCCGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACTTTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATGAGGCCACCATGCGCGTCGCCGATATGTTTATGACCt                <  2:1095986/149‑1 (MQ=255)
                                                                                                                                   gATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACAGTAGGGCTGGTTAATGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGTTCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCtt               >  2:1274040/1‑150 (MQ=255)
                                                                                                                                    aaccgaGCGACACGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCCCCATGCGCGTCGCCGATATGTTTATGACCTTc              >  1:1362084/6‑150 (MQ=255)
                                                                                                                                     tGGCAGCGCCCCGCGTGTCGCGCGGTTCGGTAATGGCCAGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGACACCATGCGCGTCGCCGATATGTTTATGACCTTcc             <  1:671913/150‑1 (MQ=255)
                                                                                                                                      ggCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCATGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGAATTGTTTATGACCTTccc            >  2:1648101/1‑150 (MQ=255)
                                                                                                                                         aGCGACCCGCGTGTCGCTCGGGTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGTTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGAc         <  1:873354/150‑1 (MQ=255)
                                                                                                                                            taCCCGCGTGGCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTc      <  1:1086937/149‑1 (MQ=255)
                                                                                                                                             aCCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCg     >  1:547182/1‑150 (MQ=255)
                                                                                                                                             aCCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCg     <  2:1616050/150‑1 (MQ=255)
                                                                                                                                              cccGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTTTTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGa    >  1:1070879/1‑150 (MQ=255)
                                                                                                                                                cgcgTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGAtt  <  2:127403/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATT  >  NC_000913/3616251‑3616544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: