BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,636,747	T→C	5.2%	T222A (ACC→GCC)	yhiN ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,747	0	T	C	5.2%	325.3 / 6.8	117	T222A (ACC→GCC)	yhiN	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (63/46); new base C (3/3); total (66/51)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e-01

Only 100 of 118 total aligned reads displayed.

CGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAA  >  NC_000913/3636599‑3636896

      tCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGCCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGttt                                                                                                                                                >  2:330280/1‑150 (MQ=255)

        aTTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTc                                                                                                                                              <  1:167863/150‑1 (MQ=255)

        aTTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTc                                                                                                                                              >  1:1731274/1‑150 (MQ=255)

        aTTCAGGAAGGATTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCTGTTGCCAGTAGCTTGAAATCTGCAACACCGCCTGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTc                                                                                                                                              <  2:1417941/150‑1 (MQ=255)

         ttCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCAGGTTGCCAGTAGCTTGAAATCTGCAACACGGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAACACGGTGCCGTTTTCa                                                                                                                                             >  1:151095/1‑150 (MQ=255)

          tCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTTAAGAGTAAGTTCTAAAGTAAAACGGTGCCGTTTTCAg                                                                                                                                            >  2:1227903/1‑150 (MQ=255)

          tCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTccg                                                                                                                                            <  1:155498/150‑3 (MQ=255)

           cAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGc                                                                                                                                           <  2:1152285/150‑1 (MQ=255)

           cAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTccgt                                                                                                                                           <  2:1257298/150‑4 (MQ=255)

           cAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGGTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGGCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGc                                                                                                                                           <  2:431761/150‑1 (MQ=255)

            aGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCg                                                                                                                                          >  1:1620956/1‑150 (MQ=255)

            aGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTccgtg                                                                                                                                          <  1:1455456/150‑5 (MQ=255)

             ggAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCGGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCgg                                                                                                                                         >  2:413491/1‑150 (MQ=255)

             ggAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCgg                                                                                                                                         >  1:1297065/1‑150 (MQ=255)

               aaGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTa                                                                                                                                       <  1:885242/150‑1 (MQ=255)

                 ggTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAAt                                                                                                                                     >  1:1148334/1‑150 (MQ=255)

                  gTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATc                                                                                                                                    >  1:763492/1‑150 (MQ=255)

                   tttCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCa                                                                                                                                   <  1:977729/150‑1 (MQ=255)

                     gcGAGGTCCACATCCGGTAGCAGACTGAAGCTGACAAATTCCACCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGGGGGTGAAGAGTAAGTTCTCACGGAAACCGGGGCCGTTTTCAGCGGTAATCACg                                                                                                                                 <  2:351977/149‑1 (MQ=255)

                        aGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTACCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGaa                                                                                                                              >  2:800369/1‑150 (MQ=255)

                         ggTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAg                                                                                                                             <  1:428452/150‑1 (MQ=255)

                           tCCACATCCGGTAGCAGATTGATGCTGCCAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAAAACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTATCAGCGGTAATAACGGAAgtc                                                                                                                           >  1:310754/1‑148 (MQ=255)

                            ccACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTAGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCAAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTCACGTTGTCAGCGGTAATCACGGAAGGcg                                                                                                                          >  1:252754/1‑149 (MQ=255)

                            ccACAACCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCa                                                                                                                          >  1:56980/1‑150 (MQ=255)

                               caTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCg                                                                                                                       >  2:746012/1‑150 (MQ=255)

                               caTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCAAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCAccc                                                                                                                       >  1:1045526/1‑149 (MQ=255)

                                    ggTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcg                                                                                                                  >  2:1130526/1‑150 (MQ=255)

                                      tAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCGGCGGTAATCACGGAAGGCACCGCCAcgcc                                                                                                                <  2:1477140/150‑1 (MQ=255)

                                           gATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCCCGCCCGCCa                                                                                                           >  2:1203249/1‑150 (MQ=255)

                                            aTTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAg                                                                                                          <  2:1297065/150‑1 (MQ=255)

                                                cggCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCAcc                                                                                                      <  1:1132051/148‑1 (MQ=255)

                                                aTGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCAcc                                                                                                      <  2:407930/150‑1 (MQ=255)

                                                  gCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTg                                                                                                    <  1:536268/150‑1 (MQ=255)

                                                    tGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTACGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTa                                                                                                  >  1:555548/1‑150 (MQ=255)

                                                      aCAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAAc                                                                                                <  2:56980/150‑1 (MQ=255)

                                                        aaaTTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAActc                                                                                              >  1:183227/1‑150 (MQ=255)

                                                         aaTTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAActct                                                                                             <  1:796476/150‑1 (MQ=255)

                                                            tCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCg                                                                                          <  2:1544708/150‑1 (MQ=255)

                                                            tCCCCCGGTTGCCAGTAGCTTGAAAGCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCg                                                                                          <  2:1618302/150‑1 (MQ=255)

                                                             cccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                         >  1:1399585/1‑150 (MQ=255)

                                                             cccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                         >  2:629262/1‑150 (MQ=255)

                                                             cccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                         >  1:785629/1‑150 (MQ=255)

                                                             cccacGGTTGCCAGTAGATTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAAACCCGGAAGGCACCGCCCCGCCCGCCAGCACCTGTAACTCTGCGa                                                                                         >  2:1037368/1‑150 (MQ=255)

                                                              ccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAg                                                                                        <  2:1410767/150‑1 (MQ=255)

                                                               cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGc                                                                                       <  1:555353/150‑1 (MQ=255)

                                                                ccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCCCCTGTAACTCTTCGAGCa                                                                                      >  1:332241/1‑150 (MQ=255)

                                                                  ggTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAAc                                                                                    >  2:767090/1‑150 (MQ=255)

                                                                  ggTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAAc                                                                                    >  1:1143482/1‑150 (MQ=255)

                                                                  ggTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAAc                                                                                    >  2:214531/1‑150 (MQ=255)

                                                                    ttGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACgg                                                                                  >  2:1643656/1‑150 (MQ=255)

                                                                       ccAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGttt                                                                               >  1:1370551/1‑150 (MQ=255)

                                                                       ccAGTAGCTTGAAATCTGCAACACCGCCGGTCCACACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCTCGGAAAACGGTGCCGTTTTCAGCGGTACTCACGGAAGGCACCGCCACGCCAGCCAGCACCTGTAACTCTTCGAGCACCGGgtt                                                                               >  2:1104203/1‑150 (MQ=255)

                                                                        cAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTa                                                                              <  2:16024/150‑1 (MQ=255)

                                                                        cAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTa                                                                              >  2:238082/1‑150 (MQ=255)

                                                                           tAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCACCGGTTTATGc                                                                           >  1:1314857/1‑150 (MQ=255)

                                                                              cTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                        >  2:504008/1‑150 (MQ=255)

                                                                               ttGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCagag                                                                       <  1:368616/150‑1 (MQ=255)

                                                                               ttGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCagag                                                                       >  1:941562/1‑150 (MQ=255)

                                                                                tGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGt                                                                      <  1:239766/150‑1 (MQ=255)

                                                                                 gAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGGTTATGCagatgg                                                                     >  2:707820/1‑147 (MQ=255)

                                                                                 gAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTCAAGAGTAAGTTCTCACGGAAACCGGTGCCGTATTCAGCTGTTATAACGGAATGCACCGCCACGCCCGCCAGCACCTGTAACTCGTCGAGCAACGGTTTTTGCAGAGtt                                                                     >  2:1041126/1‑149 (MQ=255)

                                                                                    aTCAGCAACACCGCCGGTCCACACAACCCGCGCTGGGAGAAGAGTAAGTTCTCACGGAAAACGGTGACCTATTCAGCGGTAATAACGGAAGGCACCAACACGCCAGCCAGCACCTGTAACTCATCGAGCAACGGTTTATGCAGAGAGAAt                                                                  >  2:977949/1‑150 (MQ=255)

                                                                                     tCTGCATCACCGCGGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAATCGGTGCGGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATg                                                                 <  2:153688/150‑1 (MQ=255)

                                                                                     tCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATg                                                                 <  2:795358/150‑1 (MQ=255)

                                                                                      cTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATgg                                                                >  2:375560/1‑150 (MQ=255)

                                                                                      cTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATgg                                                                >  2:651976/1‑150 (MQ=255)

                                                                                         cAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCAc                                                             >  2:1613976/1‑150 (MQ=255)

                                                                                          accagcgccgGTCCAGACAAGCCGCGGTGGGTGAAGAGGAAGTTCTCACGGAAAACGGTGCCGTTTTCAGAGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCAcc                                                            <  1:88602/145‑1 (MQ=255)

                                                                                             acCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGa                                                         >  1:1387316/1‑150 (MQ=255)

                                                                                                   ggTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcg                                                   >  2:1380516/1‑150 (MQ=255)

                                                                                                      ccAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCgg                                                <  1:483820/150‑1 (MQ=255)

                                                                                                      ccAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCgg                                                >  1:853229/1‑150 (MQ=255)

                                                                                                      ccAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCgg                                                >  2:1345285/1‑150 (MQ=255)

                                                                                                          ccAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCg                                            <  1:648233/149‑1 (MQ=255)

                                                                                                           cAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTTTGCAGAGTGAATGGCACCAGACCCGCGCGGGTCgg                                           >  2:1732516/1‑150 (MQ=255)

                                                                                                             aGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGACGgca                                         <  1:1141011/150‑1 (MQ=255)

                                                                                                              gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGAAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggtg                                        <  2:1716986/150‑6 (MQ=255)

                                                                                                              gCCGCGGTGGGTGAAGAGGAAGATCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggtg                                        <  2:1710361/150‑6 (MQ=255)

                                                                                                               ccGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggtgc                                       <  1:1027871/150‑7 (MQ=255)

                                                                                                               ccGCGGGGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCACCACCTGTATCTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTctggtgc                                       <  1:158043/150‑7 (MQ=255)

                                                                                                                 gcgGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCAc                                     >  1:436894/1‑150 (MQ=255)

                                                                                                                            agagTAAGTTCTCACGGAAAACGGTGTCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaa                          >  1:1209849/1‑150 (MQ=255)

                                                                                                                               gTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCCCGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCCGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAAtt                       >  2:1564160/1‑150 (MQ=255)

                                                                                                                                  ggTTCTTACGGAAAACGGTGCCGTTTTCAGCGGTAACCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGtt                    >  1:448240/2‑150 (MQ=255)

                                                                                                                                   gTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTc                   >  1:559335/1‑150 (MQ=255)

                                                                                                                                   gTTCTCACGGAAAACGGGGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTc                   <  2:414450/150‑1 (MQ=255)

                                                                                                                                    ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCg                  <  2:127949/150‑1 (MQ=255)

                                                                                                                                    ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCg                  <  2:1148334/150‑1 (MQ=255)

                                                                                                                                      ctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCCCCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGCCCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc                <  1:398003/150‑1 (MQ=255)

                                                                                                                                      ctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc                >  2:1332659/1‑150 (MQ=255)

                                                                                                                                      ctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc                <  2:941562/150‑1 (MQ=255)

                                                                                                                                      ctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc                <  1:1697301/150‑1 (MQ=255)

                                                                                                                                      ctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGGGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc                <  2:1731274/150‑1 (MQ=255)

                                                                                                                                         gctgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAAt             >  1:568732/4‑150 (MQ=255)

                                                                                                                                         gctgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAAt             >  1:724128/4‑150 (MQ=255)

                                                                                                                                         gctgAAAACGGCACCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGGTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATGGTTCGGCAAt             >  2:240076/4‑150 (MQ=255)

                                                                                                                                          cGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCGGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATc            >  1:308865/1‑150 (MQ=255)

                                                                                                                                              aaaCGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTCTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTtat        <  2:602704/150‑1 (MQ=255)

                                                                                                                                                ccGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGGGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATaa      <  1:547035/149‑1 (MQ=255)

                                                                                                                                                    tGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCaa  <  1:746012/150‑1 (MQ=255)

CGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAA  >  NC_000913/3636599‑3636896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`