Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,639,338 T→C 6.3% intergenic (+197/+47) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,639,3380TC 6.3% 338.2 / 9.6 127intergenic (+197/+47)pitA/uspBphosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base T (57/60);  new base C (2/6);  total (59/68)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

Only 100 of 129 total aligned reads displayed.

ACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAAT  >  NC_000913/3639189‑3639487
                                                                                                                                                     |                                                                                                                                                     
    aGTGCGCTTTGTTCATGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGGAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGt                                                                                                                                                   <  1:259142/150‑1 (MQ=255)
     gTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGtt                                                                                                                                                  >  2:1142680/1‑150 (MQ=255)
       gcgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATACGGCCTACAAAAACATGCAAATTAAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATAAGGCAATTTTGCGTTTGTAATCAGACTCGAGCGGGTCAGTTTa                                                                                                                                                >  2:1212117/1‑150 (MQ=255)
        cgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTAc                                                                                                                                               <  1:466515/150‑1 (MQ=255)
         gcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACt                                                                                                                                              <  1:619518/150‑1 (MQ=255)
         gcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACt                                                                                                                                              <  2:415614/150‑1 (MQ=255)
          cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATACGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTg                                                                                                                                             >  2:1012795/1‑150 (MQ=255)
          cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTg                                                                                                                                             <  2:438520/150‑1 (MQ=255)
               ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCTAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCg                                                                                                                                        <  2:49985/150‑1 (MQ=255)
               gtCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAACACATGCAAATTCAATCGAGTGCAGAGATTATGTCGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCg                                                                                                                                        <  2:856174/149‑1 (MQ=255)
                 cATGCCGGCTGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCATTAGATTGCAGAGATTATGTAGGACTGATAAGCGTAGAGCATCAGGCAATTTTGCGGTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCt                                                                                                                                      <  2:151414/150‑1 (MQ=21)
                 cATGCCGGATGCGGCGTGAACGCCTTATCCTGACTACAAACACATGCAAATTCAAAAGTTTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTATGCGTTGGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCt                                                                                                                                      <  1:296761/150‑1 (MQ=18)
                 cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCt                                                                                                                                      >  2:1581154/1‑150 (MQ=255)
                 cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCt                                                                                                                                      <  1:824019/150‑1 (MQ=255)
                  aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtt                                                                                                                                     <  1:395014/150‑1 (MQ=255)
                   ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCTAGCGGGTCAGTTTACTGGCCCGCttt                                                                                                                                    >  1:727398/2‑150 (MQ=21)
                    gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttt                                                                                                                                   >  1:854856/1‑150 (MQ=255)
                     ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCtcgag                                                                                                                                  <  1:1013277/150‑5 (MQ=18)
                     ccGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCttttt                                                                                                                                  >  2:657630/1‑150 (MQ=25)
                       ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGCGCGGGTCAGTTTACTGGCCCGCttttttt                                                                                                                                >  1:354865/1‑150 (MQ=255)
                        gATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTa                                                                                                                               <  1:1376660/150‑1 (MQ=255)
                         aTGCGGCTGGAACGCCTTATCCGGCCTACAAAAAACTGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGAAAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTAt                                                                                                                              <  1:77276/150‑1 (MQ=25)
                          tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATg                                                                                                                             <  2:1609067/150‑1 (MQ=255)
                          tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATg                                                                                                                             <  1:398919/150‑1 (MQ=255)
                          tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATg                                                                                                                             >  2:467412/1‑150 (MQ=255)
                           tcggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGc                                                                                                                            <  1:1096510/149‑1 (MQ=255)
                           gcggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAAGCAATTTTGCGTTTGTAATCAGTCTCGAGCCGGTCAGTTTACTGGCCCGCTTTTTTTATGc                                                                                                                            >  2:684746/1‑150 (MQ=255)
                             ggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGt                                                                                                                          >  1:1378332/1‑150 (MQ=255)
                              gcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGtt                                                                                                                         <  2:1427159/150‑1 (MQ=255)
                                gTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGttat                                                                                                                       <  2:1399705/150‑1 (MQ=255)
                                   aaCGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTtattat                                                                                                                    >  1:801260/1‑150 (MQ=255)
                                    aCGCCTTATCCGGCATTAAAAAACATGCAAATTCAATAGTTTGCATAGATTATGTAGGCCTGAGAAGCGTAGGGCATGAGGCAATTTTTCGTTTTTAATCAGTATCGAGCGGGTCTGTTTACTGGCTAGCTTTTTTTATGCGTTattatt                                                                                                                   <  2:443414/150‑1 (MQ=255)
                                     cGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTg                                                                                                                  <  1:988968/150‑1 (MQ=255)
                                      gCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGc                                                                                                                 >  2:414876/1‑150 (MQ=255)
                                       ccTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                                                                >  1:750537/1‑150 (MQ=255)
                                       ccTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                                                                >  2:415050/1‑150 (MQ=255)
                                       ccTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                                                                >  1:1482088/1‑150 (MQ=255)
                                       ccTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                                                                >  2:559356/1‑150 (MQ=255)
                                        cTTATCCGGCCTACAAAAACATGCAAATTCTATAGATTGGAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTTCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTg                                                                                                               <  1:718732/150‑1 (MQ=255)
                                        cTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTg                                                                                                               <  1:1731012/150‑1 (MQ=255)
                                        cTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTg                                                                                                               >  2:1309119/1‑150 (MQ=255)
                                            tCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGt                                                                                                           <  1:319091/150‑1 (MQ=255)
                                                  cTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTaa                                                                                                     >  2:17190/1‑150 (MQ=255)
                                                   tACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAAt                                                                                                    <  1:1150431/150‑1 (MQ=255)
                                                   tACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATg                                                                                                   >  1:211507/1‑150 (MQ=255)
                                                    aCAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATg                                                                                                   <  1:1270302/150‑1 (MQ=255)
                                                       aaaaCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCa                                                                                                >  2:1067283/1‑150 (MQ=255)
                                                         aaCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCaaa                                                                                              <  1:1134828/150‑1 (MQ=255)
                                                           cATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatc                                                                                            >  2:1447052/1‑150 (MQ=255)
                                                              gCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcata                                                                                         >  1:1505096/1‑149 (MQ=255)
                                                              gCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGGCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcatc                                                                                         <  2:857965/150‑1 (MQ=255)
                                                               cAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAAtcatca                                                                                        <  1:1171147/150‑1 (MQ=255)
                                                                  aTTCAATAGATTGCAGAGATTATGTAGGGCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGGCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATg                                                                                     <  1:1066697/150‑1 (MQ=255)
                                                                  aTTCAATAGATTGCAGAGATTATGTAGGCCTGGTAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATg                                                                                     <  2:1398901/150‑1 (MQ=255)
                                                                     cAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATAATTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAAAGCCAAATCATCAATGaaa                                                                                  >  2:778834/1‑150 (MQ=255)
                                                                        tAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCa                                                                               <  2:105903/150‑1 (MQ=255)
                                                                         aGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAg                                                                              <  1:838550/150‑1 (MQ=255)
                                                                          gATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAgg                                                                             >  2:1106361/1‑150 (MQ=255)
                                                                          gATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGGTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAgg                                                                             >  1:682022/1‑150 (MQ=255)
                                                                            ttGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCGCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCt                                                                           <  2:675395/150‑1 (MQ=255)
                                                                              gCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGa                                                                         >  2:1251316/1‑150 (MQ=255)
                                                                                agagATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGacc                                                                       >  2:1692749/1‑150 (MQ=255)
                                                                                  agaTTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccac                                                                     >  2:299898/1‑150 (MQ=255)
                                                                                     ttATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccaccac                                                                  <  2:1378332/150‑1 (MQ=255)
                                                                                     ttATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccaccac                                                                  <  2:1384989/150‑1 (MQ=255)
                                                                                       aTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTTCTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGAccaccacca                                                                >  1:1373217/1‑150 (MQ=255)
                                                                                           aGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGAcc                                                            <  1:642214/150‑1 (MQ=255)
                                                                                            ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGCCCACCACCAGACca                                                           >  2:291275/1‑150 (MQ=255)
                                                                                            ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACca                                                           >  2:885852/1‑150 (MQ=255)
                                                                                             gCCTGATAAGCGTAGCGCAACAGGCAATTTTGCGTTTGTAAACAGTCTCGAGCGGGTCACTTTACTGGCCCGCTTTTATTATGCGTTATTATTGCTGAACTCCTAAATGCCAAATCATCAATGCAATCAGTCTGACCCCCACCAGAcaaa                                                          >  2:1295711/1‑147 (MQ=38)
                                                                                                tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcacaca                                                       >  1:923445/1‑150 (MQ=255)
                                                                                                tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcacaca                                                       >  1:615462/1‑150 (MQ=255)
                                                                                                   tAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATg                                                    >  1:1570330/1‑150 (MQ=255)
                                                                                                    aaGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATgc                                                   <  2:1612651/150‑1 (MQ=255)
                                                                                                     aGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATgcg                                                  >  2:30312/1‑150 (MQ=255)
                                                                                                        gTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCtt                                                                                                       >  1:1214901/1‑94 (MQ=255)
                                                                                                         tAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTgg                                              >  2:105896/1‑150 (MQ=255)
                                                                                                          aGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGTGCTGGt                                             <  1:1155522/150‑1 (MQ=255)
                                                                                                             gcATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAg                                          >  2:1679467/1‑150 (MQ=255)
                                                                                                             gcATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAg                                          <  1:1412708/150‑1 (MQ=255)
                                                                                                              cATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGa                                         <  1:1379672/150‑1 (MQ=255)
                                                                                                              cATCAGGCAATTTTGCGTTTGTAATCAGTATCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGa                                         >  2:820186/1‑150 (MQ=255)
                                                                                                               aTCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGaa                                        <  2:829055/150‑1 (MQ=255)
                                                                                                                tCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATTCGCTGGTCAGAAt                                       <  1:871058/150‑1 (MQ=255)
                                                                                                                tCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGGCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATAATTATTTAATTCATGCTGTACAACACAAGTCTACACAATGCGCTGGTCAGAAt                                       <  2:675804/150‑1 (MQ=255)
                                                                                                                    gCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAAc                                   <  1:975398/150‑1 (MQ=255)
                                                                                                                     cAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACt                                  <  1:1211769/150‑1 (MQ=255)
                                                                                                                       aTTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGc                                <  1:530620/150‑1 (MQ=255)
                                                                                                                           tGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGAc                            <  1:692516/150‑1 (MQ=255)
                                                                                                                             cGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGc                          <  1:1664039/150‑1 (MQ=255)
                                                                                                                                ttGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCAcc                       <  2:53258/150‑1 (MQ=255)
                                                                                                                                         gTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAg              >  1:1158543/1‑150 (MQ=255)
                                                                                                                                          tctcGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAgc             <  1:1581154/150‑1 (MQ=255)
                                                                                                                                              gcccGGGCCCGTTTACTGCCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATTATCAATGCAATCAGGCTGACCACCACCATACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAgcggcg         <  2:295937/147‑1 (MQ=255)
                                                                                                                                              gAGGGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATTTTTTTTTAATTATTGGTGTACAACAGATTTCTTCACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAgcggcg         <  2:807413/150‑1 (MQ=255)
                                                                                                                                                gCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGaa       >  1:1277999/1‑150 (MQ=255)
                                                                                                                                                gCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAAATGCCGACGCACCCGCTCACAGCGGCGaa       >  1:1217546/1‑150 (MQ=255)
                                                                                                                                                gCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAAAGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGaa       >  1:1278003/1‑150 (MQ=255)
                                                                                                                                                   ggTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATaa    <  1:437400/150‑1 (MQ=255)
                                                                                                                                                     tCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAAt  <  2:1482088/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATAGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAAT  >  NC_000913/3639189‑3639487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: