Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,639,344 T→A 5.4% intergenic (+203/+41) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,639,3440TA 5.4% 384.2 / 8.8 129intergenic (+203/+41)pitA/uspBphosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base T (57/65);  new base A (2/5);  total (59/70)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

Only 100 of 133 total aligned reads displayed.

GCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATC  >  NC_000913/3639196‑3639492
                                                                                                                                                    |                                                                                                                                                    
 cgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTAc                                                                                                                                                    <  1:466515/150‑1 (MQ=255)
   cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATACGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTg                                                                                                                                                  >  2:1012795/1‑150 (MQ=255)
   cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTg                                                                                                                                                  <  2:438520/150‑1 (MQ=255)
        ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCTAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCg                                                                                                                                             <  2:49985/150‑1 (MQ=255)
        gtCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAACACATGCAAATTCAATCGAGTGCAGAGATTATGTCGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCg                                                                                                                                             <  2:856174/149‑1 (MQ=255)
          cATGCCGGATGCGGCGTGAACGCCTTATCCTGACTACAAACACATGCAAATTCAAAAGTTTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTATGCGTTGGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCt                                                                                                                                           <  1:296761/150‑1 (MQ=18)
          cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCt                                                                                                                                           >  2:1581154/1‑150 (MQ=255)
          cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCt                                                                                                                                           <  1:824019/150‑1 (MQ=255)
          cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCt                                                                                                                                           <  1:184021/150‑1 (MQ=255)
           aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtt                                                                                                                                          <  1:395014/150‑1 (MQ=255)
            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCTAGCGGGTCAGTTTACTGGCCCGCttt                                                                                                                                         >  1:727398/2‑150 (MQ=21)
             gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttt                                                                                                                                        >  1:854856/1‑150 (MQ=255)
             gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCtttt                                                                                                                                        >  2:437400/1‑150 (MQ=255)
              ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCtcgag                                                                                                                                       <  1:1013277/150‑5 (MQ=18)
              ccGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCttttt                                                                                                                                       >  2:657630/1‑150 (MQ=25)
                ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGCGCGGGTCAGTTTACTGGCCCGCttttttt                                                                                                                                     >  1:354865/1‑150 (MQ=255)
                 gATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTa                                                                                                                                    <  1:1376660/150‑1 (MQ=255)
                  aTGCGGCTGGAACGCCTTATCCGGCCTACAAAAAACTGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGAAAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTAt                                                                                                                                   <  1:77276/150‑1 (MQ=25)
                   tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATg                                                                                                                                  >  2:467412/1‑150 (MQ=255)
                   tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATg                                                                                                                                  <  2:1609067/150‑1 (MQ=255)
                   tGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATg                                                                                                                                  <  1:1324215/150‑1 (MQ=255)
                    tcggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGc                                                                                                                                 <  1:1096510/149‑1 (MQ=255)
                    gcggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAAGCAATTTTGCGTTTGTAATCAGTCTCGAGCCGGTCAGTTTACTGGCCCGCTTTTTTTATGc                                                                                                                                 >  2:684746/1‑150 (MQ=255)
                      ggcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGAATGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGt                                                                                                                               >  1:1384989/1‑150 (MQ=255)
                       gcgTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGtt                                                                                                                              <  2:1427159/150‑1 (MQ=255)
                         gTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGttat                                                                                                                            <  2:1399705/150‑1 (MQ=255)
                            aaCGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTtattat                                                                                                                         >  2:504014/1‑150 (MQ=255)
                            aaCGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTtattat                                                                                                                         >  1:801260/1‑150 (MQ=255)
                               gCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGc                                                                                                                      >  2:414876/1‑150 (MQ=255)
                                ccTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                                                                     >  1:750537/1‑150 (MQ=255)
                                ccTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                                                                     >  2:559356/1‑150 (MQ=255)
                                ccTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                                                                     >  1:660876/1‑150 (MQ=255)
                                ccTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                                                                     >  2:415050/1‑150 (MQ=255)
                                 cTTATCCGGCCTACAAAAACATGCAAATTCTATAGATTGGAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTTCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTg                                                                                                                    <  1:718732/150‑1 (MQ=255)
                                    aTCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAg                                                                                                                 <  2:39301/150‑1 (MQ=255)
                                     tCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGt                                                                                                                <  1:319091/150‑1 (MQ=255)
                                           cTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTaa                                                                                                          >  2:17190/1‑150 (MQ=255)
                                            tACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAAt                                                                                                         <  1:1150431/150‑1 (MQ=255)
                                            tACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATg                                                                                                        >  1:211507/1‑150 (MQ=255)
                                             aCAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATg                                                                                                        <  1:1270302/150‑1 (MQ=255)
                                                aaaaCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCa                                                                                                     >  2:1067283/1‑150 (MQ=255)
                                                  aaCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCaaa                                                                                                   <  1:1134828/150‑1 (MQ=255)
                                                    cATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatc                                                                                                 >  2:1447052/1‑150 (MQ=255)
                                                       gCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcata                                                                                              >  1:1505096/1‑149 (MQ=255)
                                                       gCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGGCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcatc                                                                                              <  2:857965/150‑1 (MQ=255)
                                                        cAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAAtcatca                                                                                             <  1:1171147/150‑1 (MQ=255)
                                                           aTTCAATAGATTGCAGAGATTATGTAGGGCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGGCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATg                                                                                          <  1:1066697/150‑1 (MQ=255)
                                                           aTTCAATAGATTGCAGAGATTATGTAGGCCTGGTAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATg                                                                                          <  2:1398901/150‑1 (MQ=255)
                                                              cAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATAATTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAAAGCCAAATCATCAATGaaa                                                                                       >  2:778834/1‑150 (MQ=255)
                                                                  aGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAg                                                                                   <  1:838550/150‑1 (MQ=255)
                                                                  aGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAg                                                                                   <  2:660876/150‑1 (MQ=255)
                                                                   gATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAgg                                                                                  >  2:1106361/1‑150 (MQ=255)
                                                                       gCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGa                                                                              >  2:1251316/1‑150 (MQ=255)
                                                                         agagATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGacc                                                                            >  2:1692749/1‑150 (MQ=255)
                                                                           agaTTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccac                                                                          >  2:299898/1‑150 (MQ=255)
                                                                              ttATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccaccac                                                                       <  2:1384989/150‑1 (MQ=255)
                                                                              ttATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccaccac                                                                       <  2:1378332/150‑1 (MQ=255)
                                                                                aTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTTCTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGAccaccacca                                                                     >  1:1373217/1‑150 (MQ=255)
                                                                                    aGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGAcc                                                                 <  1:642214/150‑1 (MQ=255)
                                                                                     ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGCCCACCACCAGACca                                                                >  2:291275/1‑150 (MQ=255)
                                                                                     ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACca                                                                >  2:885852/1‑150 (MQ=255)
                                                                                      gCCTGATAAGCGTAGCGCAACAGGCAATTTTGCGTTTGTAAACAGTCTCGAGCGGGTCACTTTACTGGCCCGCTTTTATTATGCGTTATTATTGCTGAACTCCTAAATGCCAAATCATCAATGCAATCAGTCTGACCCCCACCAGAcaaa                                                               >  2:1295711/1‑147 (MQ=38)
                                                                                         tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcacaca                                                            >  1:923445/1‑150 (MQ=255)
                                                                                         tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcacaca                                                            >  1:615462/1‑150 (MQ=255)
                                                                                            tAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATg                                                         >  1:1570330/1‑150 (MQ=255)
                                                                                             aaGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATgc                                                        <  2:1612651/150‑1 (MQ=255)
                                                                                                 gTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCtt                                                                                                            <  2:1214901/94‑1 (MQ=255)
                                                                                                 gTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCtt                                                                                                            >  1:1214901/1‑94 (MQ=255)
                                                                                                  tAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTgg                                                   >  2:105896/1‑150 (MQ=255)
                                                                                                   aGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGTGCTGGt                                                  <  1:1155522/150‑1 (MQ=255)
                                                                                                    gcgcATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTc                                                 <  1:400898/150‑1 (MQ=255)
                                                                                                      gcATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAg                                               <  1:1412708/150‑1 (MQ=255)
                                                                                                      gcATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAg                                               >  2:1679467/1‑150 (MQ=255)
                                                                                                       cATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGa                                              <  1:1251316/150‑1 (MQ=255)
                                                                                                       cATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGa                                              <  1:1379672/150‑1 (MQ=255)
                                                                                                       cATCAGGCAATTTTGCGTTTGTAATCAGTATCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGa                                              >  2:820186/1‑150 (MQ=255)
                                                                                                        aTCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGaa                                             <  2:829055/150‑1 (MQ=255)
                                                                                                         tCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGGCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATAATTATTTAATTCATGCTGTACAACACAAGTCTACACAATGCGCTGGTCAGAAt                                            <  2:675804/150‑1 (MQ=255)
                                                                                                             gCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAAc                                        <  1:975398/150‑1 (MQ=255)
                                                                                                              cAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACt                                       <  1:1211769/150‑1 (MQ=255)
                                                                                                                aTTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGc                                     <  1:530620/150‑1 (MQ=255)
                                                                                                                    tGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGAc                                 <  1:692516/150‑1 (MQ=255)
                                                                                                                      cGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGc                               <  1:1664039/150‑1 (MQ=255)
                                                                                                                             aaTCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCt                        <  1:89844/150‑1 (MQ=255)
                                                                                                                                  gTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAg                   >  1:1158543/1‑150 (MQ=255)
                                                                                                                                   tctcGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAgc                  <  1:1581154/150‑1 (MQ=255)
                                                                                                                                       gcccGGGCCCGTTTACTGCCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATTATCAATGCAATCAGGCTGACCACCACCATACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAgcggcg              <  2:295937/147‑1 (MQ=255)
                                                                                                                                       gAGGGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATTTTTTTTTAATTATTGGTGTACAACAGATTTCTTCACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAgcggcg              <  2:807413/150‑1 (MQ=255)
                                                                                                                                        aGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGTAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGa             >  1:994571/1‑150 (MQ=255)
                                                                                                                                        aGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGa             >  1:602069/1‑150 (MQ=255)
                                                                                                                                         gCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGaa            >  1:1277999/1‑150 (MQ=255)
                                                                                                                                           gggTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATa          >  2:1069801/1‑150 (MQ=255)
                                                                                                                                            ggTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATaa         <  1:437400/150‑1 (MQ=255)
                                                                                                                                              tCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAAt       <  2:1482088/150‑1 (MQ=255)
                                                                                                                                                 ttttACTGGCCCGATTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATTTTTTATTAATTAATGCTGTACAACAGCTTTCTACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATtca    <  2:1314050/149‑1 (MQ=255)
                                                                                                                                                 gTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATtca    >  2:439534/1‑150 (MQ=255)
                                                                                                                                                 gTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATtca    <  1:630619/150‑1 (MQ=255)
                                                                                                                                                  tttACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATtcat   <  1:1517505/150‑1 (MQ=255)
                                                                                                                                                   ttACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATtcatc  <  1:1485613/150‑1 (MQ=255)
                                                                                                                                                   ttACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGCCGAATAAATtaata  >  2:1311684/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATC  >  NC_000913/3639196‑3639492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: