Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 457,654 T→A 6.4% Y77N (TAC→AAC)  clpX → ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913457,6540TA 6.4% 353.9 / 6.9 122Y77N (TAC→AAC) clpXATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base T (58/54);  new base A (3/6);  total (62/60)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAA  >  NC_000913/457507‑457801
                                                                                                                                                   |                                                                                                                                                   
cTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTAc                                                                                                                                                   >  1:50953/1‑150 (MQ=255)
cTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTAc                                                                                                                                                   <  2:1655761/150‑1 (MQ=255)
 tGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACg                                                                                                                                                  <  2:1733275/150‑1 (MQ=255)
  gATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGt                                                                                                                                                 >  2:1633095/1‑150 (MQ=255)
      gCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGATGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATc                                                                                                                                             >  1:1014118/1‑150 (MQ=255)
      gCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATc                                                                                                                                             >  1:1393784/1‑150 (MQ=255)
       ccGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCg                                                                                                                                            <  1:284783/150‑1 (MQ=255)
         ggTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGc                                                                                                                                          >  2:1730848/1‑150 (MQ=255)
            ccATCCGTGTATATCTGCTACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACTCAGTGCGCTACCGACGCCGCATGAACTTCGCAACCACCTGGCCGATAACGTAATCGTCCAg                                                                                                                                       <  2:858401/150‑1 (MQ=255)
            ccATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGCCGATAACGTAATCGTCCAg                                                                                                                                       <  1:439070/150‑1 (MQ=255)
             cATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTTAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAgg                                                                                                                                      >  2:322241/1‑150 (MQ=255)
             cATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGCCGATAACGTAATCGTCCAgg                                                                                                                                      <  2:29461/150‑1 (MQ=255)
             cATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAgg                                                                                                                                      >  2:157363/1‑150 (MQ=255)
             cATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAgg                                                                                                                                      <  1:771360/150‑1 (MQ=255)
             cATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGGTGCACCGCATCGTGAACGCAGTGCGCTACCGAATCCGCATGAAATTCGCAACCACCTGGCCGATAACGTAATCGTCCAgg                                                                                                                                      <  2:440537/150‑1 (MQ=255)
                ccGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGCCGATAACGTAATCGTCCAggtgg                                                                                                                                   <  1:1406150/150‑4 (MQ=255)
                 cGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACa                                                                                                                                  >  2:953942/1‑150 (MQ=255)
                  gggtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAg                                                                                                                                 <  2:192909/148‑1 (MQ=255)
                   ggtATAGCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAgg                                                                                                                                <  2:691623/149‑1 (MQ=255)
                    gtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGc                                                                                                                               <  1:414346/150‑1 (MQ=255)
                    gtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGc                                                                                                                               >  1:1631295/1‑150 (MQ=255)
                       tatCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGCCGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGaa                                                                                                                            >  1:1175555/1‑150 (MQ=255)
                            gCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAGCGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAg                                                                                                                       <  2:367949/150‑1 (MQ=255)
                            gCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAg                                                                                                                       >  2:1128288/1‑150 (MQ=255)
                            gCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAg                                                                                                                       <  1:706332/150‑1 (MQ=255)
                               acgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGc                                                                                                                    >  2:1439044/1‑150 (MQ=255)
                               acgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACAGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGc                                                                                                                    >  1:1055831/1‑150 (MQ=255)
                                 gaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTg                                                                                                                  <  2:360436/150‑1 (MQ=255)
                                  aaTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTgg                                                                                                                 <  1:483097/150‑1 (MQ=255)
                                   aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGc                                                                                                                <  1:904991/150‑1 (MQ=255)
                                   aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGc                                                                                                                <  1:911036/150‑1 (MQ=255)
                                   aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGc                                                                                                                <  2:609284/150‑1 (MQ=255)
                                          gATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTcgcg                                                                                                         <  1:153721/150‑1 (MQ=255)
                                            tttATGTAACGAGATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGt                                                                                                       <  1:690456/150‑1 (MQ=255)
                                            tttATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGt                                                                                                       <  1:987262/150‑1 (MQ=255)
                                             ttATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGta                                                                                                      <  1:1544579/150‑1 (MQ=255)
                                               aTGTAACGAAATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCAAGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGATGGCGGTCGCGGtata                                                                                                    >  2:1583012/1‑150 (MQ=255)
                                                tGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATAc                                                                                                   <  2:1423126/150‑1 (MQ=255)
                                                tGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATAc                                                                                                   <  2:1037646/150‑1 (MQ=255)
                                                 gTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCTAATGAAATTCGAAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAACTGCTGGCGGTCGCGGTATACa                                                                                                  >  1:831104/1‑150 (MQ=255)
                                                 gTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGtataaa                                                                                                  >  2:860289/1‑148 (MQ=255)
                                                 gTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACa                                                                                                  >  2:594136/1‑150 (MQ=255)
                                                  tAACGACATCATTCGCGAAGAGATTAAAGAAGATGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACaa                                                                                                 <  1:1252836/150‑1 (MQ=255)
                                                   aaCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAAc                                                                                                <  2:817540/150‑1 (MQ=255)
                                                     cGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCAt                                                                                             >  1:33060/1‑150 (MQ=255)
                                                      gACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCAt                                                                                             <  2:971842/150‑1 (MQ=255)
                                                      gACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCAt                                                                                             >  1:232184/1‑150 (MQ=255)
                                                            atTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACaaa                                                                                       >  2:950314/1‑150 (MQ=255)
                                                               cgcgAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGt                                                                                    >  1:970517/1‑150 (MQ=255)
                                                                gcgAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTc                                                                                   >  2:1308101/1‑150 (MQ=255)
                                                                  taaGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTg                                                                                 <  2:1190527/149‑1 (MQ=255)
                                                                        aTTAAAGCAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACCAACGTCTGCGCCAc                                                                           >  1:133002/1‑150 (MQ=255)
                                                                        aTTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAAc                                                                           >  1:1289764/1‑150 (MQ=255)
                                                                         ttAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGCACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACTACCATTACAAACGTCTGCACAACg                                                                          >  2:879749/1‑150 (MQ=255)
                                                                          tAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACgg                                                                         >  2:961432/1‑150 (MQ=255)
                                                                           aaaGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGc                                                                        >  1:1393476/1‑150 (MQ=255)
                                                                           aaaGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATGACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACCACCATTACAAACGTCTGCGCAACGGc                                                                        >  1:241758/1‑150 (MQ=255)
                                                                               aagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATa                                                                    >  2:1174376/1‑150 (MQ=255)
                                                                                agTTGCCCCGCATCGTGAACGCAGTCCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATAc                                                                   <  2:696121/150‑1 (MQ=255)
                                                                                agTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATAc                                                                   >  1:411160/1‑150 (MQ=255)
                                                                                 gTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATAcc                                                                  <  1:1439044/150‑1 (MQ=255)
                                                                                 gTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATAcc                                                                  <  2:895892/150‑1 (MQ=255)
                                                                                   tGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAg                                                                <  2:1394318/150‑1 (MQ=255)
                                                                                     cACCGCATCGTGAACGCAGTGAGCTACCGCAGCAGCATGAAATTCGCAACCACCTGGACGATAACGATATCCGCCAGGAACAGGCGAAAAAAGTGCTGCCGGTCGCGGTATACAACCAGTACAACCGTCTGCGCAACGGCGATACCAGCa                                                              >  1:1130315/1‑150 (MQ=255)
                                                                                       ccGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAAt                                                            >  2:390433/1‑150 (MQ=255)
                                                                                        cGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACGGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATg                                                           >  1:1015972/1‑150 (MQ=255)
                                                                                         gCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATgg                                                          <  1:594406/150‑1 (MQ=255)
                                                                                         gCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATgg                                                          <  1:306991/150‑1 (MQ=255)
                                                                                           aTCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCg                                                        >  2:178393/1‑150 (MQ=255)
                                                                                             cGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCAGGACGATTACGTTATCGGCCAGGAACAGGCGAATAAAGTGCTGGCGGTCGCGGTATAGAACCATAACAAACGTCTGCGCAACGGCGATACCAGCAAtcgtgac                                                      >  2:862506/1‑144 (MQ=255)
                                                                                                gAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAACCGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAg                                                   >  2:647776/1‑150 (MQ=255)
                                                                                                 aaCGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGt                                                  <  1:1475396/150‑1 (MQ=255)
                                                                                                   cGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTg                                                >  2:599228/1‑150 (MQ=255)
                                                                                                   cGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTg                                                <  1:1707634/150‑1 (MQ=255)
                                                                                                    gCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTgg                                               <  1:76159/150‑1 (MQ=255)
                                                                                                    gCAGTGCGCTACCGACGCCGCAGGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTgg                                               <  2:1486299/150‑1 (MQ=255)
                                                                                                     cAGTGCGCTACCGACTCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTggg                                              <  1:741702/150‑1 (MQ=255)
                                                                                                     cAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTggg                                              <  1:157363/150‑1 (MQ=255)
                                                                                                     cAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTggg                                              <  1:953942/150‑1 (MQ=255)
                                                                                                         gcgcTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCaaa                                          >  1:1311734/1‑150 (MQ=255)
                                                                                                            cTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGGAACGCCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGt                                       >  2:477642/1‑150 (MQ=255)
                                                                                                            cTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGt                                       >  2:457826/1‑150 (MQ=255)
                                                                                                            cTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGt                                       <  2:590624/150‑1 (MQ=255)
                                                                                                            cTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGt                                       >  2:1302135/1‑150 (MQ=255)
                                                                                                               ccGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAAc                                    >  2:1493510/1‑150 (MQ=255)
                                                                                                                cGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACa                                   <  1:1227666/150‑1 (MQ=255)
                                                                                                                     ccgcATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGAGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTctg                              >  1:1192541/1‑150 (MQ=255)
                                                                                                                      cgcATGAAATTCGCAACCACCTGGACGAATACGTTATCGGCCAGGAACAGGCGAAAAAAGGGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTctgc                             <  1:41801/150‑1 (MQ=255)
                                                                                                                        cATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTctgctg                           >  1:482895/1‑150 (MQ=255)
                                                                                                                          tGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGAt                         >  1:1621310/1‑150 (MQ=255)
                                                                                                                           gAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATc                        <  1:1731713/150‑1 (MQ=255)
                                                                                                                           gAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTACGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATc                        <  2:1412990/150‑1 (MQ=255)
                                                                                                                           gAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATAAAAACATTACAAACGTCTGCGCAACGGCGATAACAGCAATGGCGTGGAGGTGGGCAAAAGGATCATTCTGCTGATc                        >  2:81621/1‑150 (MQ=255)
                                                                                                                            aaaTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCg                       >  1:711664/1‑150 (MQ=255)
                                                                                                                                  gCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGa                 >  2:1411528/1‑150 (MQ=255)
                                                                                                                                     tgttccTGGCCGATAACGTAATCGTCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCg              >  2:1578384/5‑150 (MQ=255)
                                                                                                                                          cTGGACGATTACGTTATCGTCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACATAGGTCTGCGCATCGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTcc         <  2:1107266/150‑1 (MQ=255)
                                                                                                                                            ggACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCgg       <  2:411160/150‑1 (MQ=255)
                                                                                                                                              aCGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTa     <  2:1346951/150‑1 (MQ=255)
                                                                                                                                              aCGAGTACGTTCTCGGCCAGTAACAGGCGAAAAAAGGGCTGGCGGTCGCGGTATACAACCATTCAAAACGGCTGCGCAACGGCGATAACAGCATTGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTa     <  2:1346955/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGATTGCCGGTCCATCCGTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAA  >  NC_000913/457507‑457801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: