BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,643,235	C→G	6.9%	G650R (GGC→CGC) ‡	prlC ←	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,643,235	0	C	G	6.9%	308.0 / 12.1	101	G650R (GGC→CGC) ‡	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand): ref base C (42/51); new base G (2/5); total (45/56)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

Only 100 of 109 total aligned reads displayed.

AAGGCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGC  >  NC_000913/3643086‑3643384

aaGGCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGcc                                                                                                                                                       >  2:1690323/1‑150 (MQ=255)

  ggCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGcccc                                                                                                                                                     >  2:1011028/1‑148 (MQ=255)

  ggCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCCGAACCGCCAc                                                                                                                                                     >  2:907023/1‑150 (MQ=255)

        ggagacGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCa                                                                                                                                               >  1:686518/6‑150 (MQ=255)

         tctcCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAg                                                                                                                                              >  1:1687488/1‑150 (MQ=255)

          ctcCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGa                                                                                                                                             <  1:1137058/150‑1 (MQ=255)

          ctcCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCACCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCAGGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGCACCGCCACGGCTCAGa                                                                                                                                             >  1:230885/1‑150 (MQ=255)

            ccGGTGCCTGTTTCATCAATTAATCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAGCCGTGGCGGTTCAGaag                                                                                                                                           <  2:1653989/150‑2 (MQ=255)

            ccGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGTCTCTTCTGAACCGCCACGGCTCAGAAt                                                                                                                                           <  1:711306/150‑1 (MQ=255)

              gtaggcTGTTTCATCAATTAAGCAGATTTTCACTGATTGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAAtgt                                                                                                                                         <  2:124774/145‑1 (MQ=255)

               gTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAGCCGTGGCGGTTCAGaagagc                                                                                                                                        <  1:449/150‑5 (MQ=255)

                tGCCTGTTTCATCTATTAAGCAGATTTTCACTGAATGAGCAGCCCTTAATGCCGTAATGCTCCAGCATCTCATCCAGCTGCGGTTCACGACCACGCAAGCGTTTGAACAGATCCATCGGCTCTTCTGAGCCGTGGCGGTTCAGaagagcc                                                                                                                                       <  2:1506532/150‑6 (MQ=255)

                tGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAGCCGTGGCGGTTCAGaagagcc                                                                                                                                       <  2:910639/150‑6 (MQ=255)

                tGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAGCCGTGGCGGTTCAGaagagcc                                                                                                                                       <  1:1628859/150‑6 (MQ=255)

                tGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGAGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCAACGGCTCAGAAttttt                                                                                                                                       >  2:1497405/1‑149 (MQ=255)

                     gTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAg                                                                                                                                  >  1:525754/1‑150 (MQ=255)

                         catcaATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAAc                                                                                                                              >  1:1171566/1‑150 (MQ=255)

                          atcaATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACg                                                                                                                             <  2:1473951/150‑1 (MQ=255)

                            caATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGGAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCCCGGCTCAGAATGTTGTCGAGGAACGAc                                                                                                                           >  1:234612/1‑150 (MQ=255)

                             aaTTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGCACAGATCCATCGGCTCTTCTGAAACGCCACGGATCAGAATGTTGTCGAGGAACGCCt                                                                                                                          >  2:152447/1‑150 (MQ=255)

                             aaTTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACt                                                                                                                          >  2:907054/1‑150 (MQ=255)

                             aaTTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACt                                                                                                                          <  1:18132/150‑1 (MQ=255)

                             aaTTAACCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACt                                                                                                                          <  1:24156/150‑1 (MQ=255)

                              aTTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGGCGAGGAACGACTg                                                                                                                         <  2:49520/150‑1 (MQ=255)

                                tAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACAACTGcc                                                                                                                       >  2:1381437/1‑150 (MQ=255)

                                  aGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCg                                                                                                                     <  1:675718/150‑1 (MQ=255)

                                   gCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTATGAACAGATCCATCGGCTCTACGGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCgg                                                                                                                    >  2:593419/1‑150 (MQ=255)

                                    cAGATTTTCACTTAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTATGAACCGCCACGTCTCAGAATGTTTTAGAGTCACGACTGCCCGGt                                                                                                                   >  1:1272845/1‑150 (MQ=255)

                                       aTTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGGTCACGACCACGGAAGCGTTTGAACAGCTCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTc                                                                                                                <  1:471247/150‑1 (MQ=255)

                                         tttCACTGAATGATCCGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCAc                                                                                                              <  2:880658/150‑1 (MQ=255)

                                            cACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTTAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGt                                                                                                           >  2:1204370/1‑150 (MQ=255)

                                            cACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGt                                                                                                           >  2:642227/1‑150 (MQ=255)

                                             aCTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGtt                                                                                                          >  1:1348875/1‑150 (MQ=255)

                                               tGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGa                                                                                                        <  2:1666372/150‑1 (MQ=255)

                                                 aaTGATCAGCCCTAAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTACAAGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGATCAGAATGATGTCGAGGAACGACTGCCCGGTTTCACGGTTGaaa                                                                                                      <  1:876068/150‑1 (MQ=255)

                                                      tCAGCCCTTACTGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGcc                                                                                                 <  1:347752/150‑1 (MQ=255)

                                                      tCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGcc                                                                                                 <  2:609885/150‑1 (MQ=255)

                                                      tCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGAGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGcc                                                                                                 <  2:1056168/150‑1 (MQ=255)

                                                       cAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACAACTGCCCGGTTTCACGGTTGAAAATGccc                                                                                                <  1:1381437/150‑1 (MQ=255)

                                                        aGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCct                                                                                               <  2:1315746/150‑1 (MQ=255)

                                                           ccTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGCCTGCCCGGTTTCACGGTTGAAAATGCCCTCtt                                                                                            >  1:1551821/1‑150 (MQ=255)

                                                              tAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCt                                                                                         <  2:529687/150‑1 (MQ=255)

                                                              tAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCt                                                                                         <  2:460882/150‑1 (MQ=255)

                                                                   ccGTAATGCTCCCGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCATAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAg                                                                                    >  2:824813/1‑150 (MQ=255)

                                                                   ccGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCCTCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAATGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAg                                                                                    >  1:1078017/1‑150 (MQ=255)

                                                                   ccGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCATAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAg                                                                                    >  2:484874/1‑150 (MQ=255)

                                                                   ccGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAg                                                                                    <  1:510819/150‑1 (MQ=255)

                                                                     gTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAgcg                                                                                  <  2:686518/150‑1 (MQ=255)

                                                                       aaTGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTGCACGGTTGAAAATGCCCTCTTCCTCAAAGcgcg                                                                                <  2:539391/150‑1 (MQ=255)

                                                                           cTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGaa                                                                            >  2:942043/1‑150 (MQ=255)

                                                                             ccAGCATCGCATCCAGCTGCGGTTCATGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAg                                                                          <  2:200302/150‑1 (MQ=255)

                                                                             ccAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGCACCGCCACGGCTCACAATGAAGTCGAGCAGCGACTGCCCGGATTCTCGGTTGAAAATGCCCACTACCTCAAAGCGCGAGAAAg                                                                          >  1:129903/1‑150 (MQ=255)

                                                                                  atcgcatcCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCt                                                                     <  1:1497405/150‑1 (MQ=255)

                                                                                   tcgcatcCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATTCACTCTTCCTCAAAGCGAGAGAAAGCAACTg                                                                    >  2:268260/1‑150 (MQ=255)

                                                                                   tcgcatcCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATATg                                                                    <  2:1153204/150‑1 (MQ=255)

                                                                                    cgcatcCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAACCGCGAGAAAGCATCTgc                                                                   >  2:83137/1‑150 (MQ=255)

                                                                                      catcCAGCTGCGGTTCACGACCACTGAAGAGTTTTAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGTTTTCACGGTTGAAATTGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTgccg                                                                 <  1:833733/150‑1 (MQ=255)

                                                                                      catcCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTgccg                                                                 <  1:531870/150‑1 (MQ=255)

                                                                                      catcCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTgccg                                                                 <  1:1669656/150‑1 (MQ=255)

                                                                                                   ttCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGc                                                    >  1:772259/1‑150 (MQ=255)

                                                                                                   ttCACGACCACGGAAGCGGTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGc                                                    <  1:269086/150‑1 (MQ=255)

                                                                                                    tCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGcc                                                   <  1:580294/150‑1 (MQ=255)

                                                                                                     cACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCCCGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGccc                                                  >  1:1329597/1‑150 (MQ=255)

                                                                                                       cGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCcac                                                >  2:1667505/1‑150 (MQ=255)

                                                                                                        gACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACTGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAACCcaca                                               <  2:1362314/150‑1 (MQ=255)

                                                                                                        gACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCcaca                                               >  1:1553807/1‑150 (MQ=255)

                                                                                                        gACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCcaca                                               <  1:1522943/150‑1 (MQ=255)

                                                                                                           cACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGt                                            <  1:441491/150‑1 (MQ=255)

                                                                                                               gAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCt                                        >  1:316226/1‑150 (MQ=255)

                                                                                                                aaGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTg                                       <  2:1496480/150‑1 (MQ=255)

                                                                                                                 aGCGTTTGAACTGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTGGGCGAGGACCGACTGCCCGGTTTCACGGTTGAAAATGCCCTCATCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTgt                                      <  2:842131/150‑1 (MQ=255)

                                                                                                                 aGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGGTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAACGCATCTGCCGCCACTACGTCAGCCCACAGGTAGCTgt                                      >  1:231806/1‑150 (MQ=255)

                                                                                                                    gTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGCGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCCCAGGTAGCTgtagt                                   >  1:1216710/1‑150 (MQ=255)

                                                                                                                       tGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAAc                                <  1:1297563/150‑1 (MQ=255)

                                                                                                                        gaccAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTTTCTAGTTACGACTGCCAGGTTACACGGTTTAATATGCCCTCTTCCTCAAAGCGGGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGAAGCTGTAGTAAcc                               <  1:1068374/147‑1 (MQ=255)

                                                                                                                        gAACAGATCCATCGGCTCTTCTGAACCGCCCCGGCTCAGAATGTTGTCGGGGCAGGACTGCCGGGTATCCGGGTTGAAAATGCCCACTTCCTCAAAGCGCGAGCAAGCATCTGCCGCCAGTACGACAGCCCCCAGGTAGCAGTATTAccc                               >  2:242801/1‑150 (MQ=255)

                                                                                                                         aaCAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCt                              <  2:1551821/150‑1 (MQ=255)

                                                                                                                         aaCAGATCCAACGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAAccg                              >  1:1443213/1‑149 (MQ=255)

                                                                                                                            aGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGCCTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCg                           >  1:514346/1‑150 (MQ=255)

                                                                                                                              aaccATCGGCTCTTCTGAACCGCCACGGCTCAGAATTTTGTCGACGAACGACTGCCCGGTTTCACGGGTGAAAATGCCCTCTTCCTCAAAGCGCGAGATCGCATCTGCCGCCATTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGc                         <  2:234612/148‑1 (MQ=255)

                                                                                                                               tCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCa                        <  2:906951/150‑1 (MQ=255)

                                                                                                                                ccATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCTCCAGTACGTCAGCCCACAGGTATCTGTTGTAACCTCCGGCAt                       >  2:1052067/1‑150 (MQ=255)

                                                                                                                                ccATCGGCTCTTCCGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCAt                       <  1:907023/150‑1 (MQ=255)

                                                                                                                                  aTCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATaa                     <  2:1288589/150‑1 (MQ=255)

                                                                                                                                  aTCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATaa                     <  1:679139/150‑1 (MQ=255)

                                                                                                                                   tCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAac                    <  2:1652523/150‑1 (MQ=255)

                                                                                                                                   tCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAac                    <  1:907054/150‑1 (MQ=255)

                                                                                                                                    cGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGATAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAacc                   >  2:352308/1‑150 (MQ=255)

                                                                                                                                    cGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAacc                   <  1:973317/150‑1 (MQ=255)

                                                                                                                                        tcttctGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCg               <  2:1348875/150‑1 (MQ=255)

                                                                                                                                         attctGAGCCGTGGCGGTTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCgg              >  1:1412425/2‑150 (MQ=255)

                                                                                                                                             tGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCCCAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGaa          >  1:1557860/1‑150 (MQ=255)

                                                                                                                                               aaCCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGaaaa        <  2:1687488/150‑1 (MQ=255)

                                                                                                                                                   gCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCAGAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATg    >  2:213194/1‑150 (MQ=255)

                                                                                                                                                     cACGGCTCAGATTGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGc  <  2:1463989/150‑1 (MQ=255)

                                                                                                                                                     cACGGCTCAGAATGTTGTCGAGGAATGACTGCCCGGTTTCACTGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGc  <  2:1078017/150‑1 (MQ=255)

                                                                                                                                                     cACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGc  >  2:968297/1‑150 (MQ=255)

                                                                                                                                                     cACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGc  <  1:1327736/150‑1 (MQ=255)

                                                                                                                                                     cACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGc  <  2:512497/150‑1 (MQ=255)

                                                                                                                                                     cACGGCTCAGAATGTTGGCGAGGAACGACTGCCCGGTTTCACGGGTGAAAATGCCCTCTTCCTCAAAGCGAGAGAAAGCATCTTCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGc  <  1:152447/150‑1 (MQ=255)

AAGGCACCGTCTCCGGTGCCTGTTTCATCAATTAAGCAGATTTTCACTGAATGATCAGCCCTTAATGCCGTAATGCTCCAGCATCGCATCCAGCTGCGGTTCACGACCACGGAAGCGTTTGAACAGATCCATCGGCTCTTCTGAACCGCCACGGCTCAGAATGTTGTCGAGGAACGACTGCCCGGTTTCACGGTTGAAAATGCCCTCTTCCTCAAAGCGCGAGAAAGCATCTGCCGCCAGTACGTCAGCCCACAGGTAGCTGTAGTAACCTGCGGCATAACCACCGGCGAAAATATGGC  >  NC_000913/3643086‑3643384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`