Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,655,922 C→T 7.0% intergenic (‑20/+44) yhiD ← / ← hdeB putative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,9220CT 7.0% 439.9 / 13.6 142intergenic (‑20/+44)yhiD/hdeBputative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein
Reads supporting (aligned to +/- strand):  ref base C (73/59);  new base T (5/5);  total (78/64)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 146 total aligned reads displayed.

GGGCGCTTCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATC  >  NC_000913/3655773‑3656067
                                                                                                                                                     |                                                                                                                                                 
gggCGCTTCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGagc                                                                                                                                                   >  1:952892/1‑150 (MQ=255)
 ggCGCTTCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGagca                                                                                                                                                  <  1:1406742/150‑1 (MQ=255)
    gcTTCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAgcagca                                                                                                                                               <  2:1268902/150‑1 (MQ=255)
     cTTCCCATGACAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCaa                                                                                                                                              <  2:630447/150‑1 (MQ=255)
       tCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGa                                                                                                                                            <  1:497843/150‑1 (MQ=255)
         ccATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGGCTTTCCATGCCAATAGCGCCACAGGCAATTGACGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATg                                                                                                                                          <  2:737044/150‑1 (MQ=255)
           aTGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGc                                                                                                                                        <  1:1425920/150‑1 (MQ=255)
           aTGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGACAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGc                                                                                                                                        >  1:819367/1‑150 (MQ=255)
              ccAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCa                                                                                                                                     <  1:854462/150‑1 (MQ=255)
               cAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCaa                                                                                                                                    >  2:1164619/1‑150 (MQ=255)
                aaTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCAAATAGCGCCACAGGCAATTGCCGCGAGTATAAGAAGTATATTAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAAc                                                                                                                                   >  1:80791/1‑150 (MQ=255)
                 aTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATATTAAAATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACt                                                                                                                                  >  1:449736/1‑150 (MQ=255)
                 aTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACt                                                                                                                                  >  1:1319876/1‑150 (MQ=255)
                   tAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTg                                                                                                                                >  1:1567062/1‑150 (MQ=255)
                    aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                               >  1:601000/1‑150 (MQ=255)
                    aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                               >  1:416551/1‑150 (MQ=255)
                    aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                               >  1:1008689/1‑150 (MQ=255)
                     aTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGGATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCt                                                                                                                              <  2:1500865/150‑1 (MQ=255)
                      aaCATGGGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCCTTCCCTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATACTAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGTTGCGCCATCTTGCtg                                                                                                                             <  1:308747/149‑2 (MQ=255)
                       aCATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTTCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTAc                                                                                                                            <  1:1305387/150‑1 (MQ=255)
                          tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                         <  2:1356850/150‑1 (MQ=39)
                          tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                         <  2:140190/150‑1 (MQ=39)
                           ttgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGTAAATTGCCGCGAGTATAAGACGTATAATAAATTCTTCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCt                                                                                                                        <  1:1001279/149‑1 (MQ=255)
                            tgtgCGTAACCCTGCTCCATTGCCGCGCATTTGCCTATCCATGCGAATAGCGCCACTGGCATTTGCCGCGAGTATAAGACGTCTAATAAATTCTGCTGTCATATAAAACTGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGATGCTCCtt                                                                                                                       <  1:1068863/150‑1 (MQ=255)
                               gCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCttttt                                                                                                                    >  1:1265598/1‑150 (MQ=255)
                                 gTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCtttttgc                                                                                                                  <  1:1500065/150‑3 (MQ=38)
                                   aaCCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACtt                                                                                                                <  1:631348/150‑1 (MQ=255)
                                     cccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGc                                                                                                              <  2:1436492/150‑1 (MQ=255)
                                      ccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCTCAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCATGATGGCTCAACTTGATACTCCTTTTTACTTTCa                                                                                                             >  1:1222635/1‑150 (MQ=255)
                                       cTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCACCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAc                                                                                                            >  1:1343993/1‑150 (MQ=255)
                                             cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAACGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                                                                                      <  1:1068701/150‑1 (MQ=255)
                                             cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                                                                                      >  2:171099/1‑150 (MQ=255)
                                              tttGCCGCGCATTTGCCTTTCCATGCCAATATCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGGCATATAAAAAAGCAAAAAGGATCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                                                                                     <  1:157940/150‑1 (MQ=255)
                                                 gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                  >  2:1488352/1‑150 (MQ=255)
                                                 gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                  >  1:1414215/1‑150 (MQ=255)
                                                 gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                  >  2:479791/1‑150 (MQ=255)
                                                  ccGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTc                                                                                                 <  2:51394/150‑1 (MQ=255)
                                                   ctcgcATATGCCTTTCAATGTCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGCCGTATAATAAATTCTGATGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                <  2:292996/148‑1 (MQ=255)
                                                   cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                >  1:1349447/1‑150 (MQ=255)
                                                   cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                <  1:70555/150‑1 (MQ=255)
                                                   cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTATTTACTTGCACCTCATTAAttag                                                                                                >  1:229402/1‑148 (MQ=255)
                                                     cgcATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGc                                                                                              <  1:1028545/150‑1 (MQ=255)
                                                        ctttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACCTATAATAACATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCCAGATGGCTCAACTCGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                                                                           <  2:859830/149‑1 (MQ=255)
                                                            gCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTAGCACCTCATTAATTCGGCAAGTCAt                                                                                       >  2:1071377/1‑150 (MQ=255)
                                                             ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCAtt                                                                                      <  2:1008689/150‑1 (MQ=255)
                                                              cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                     >  2:201236/1‑150 (MQ=255)
                                                              cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                     >  2:1412964/1‑150 (MQ=255)
                                                                  ccATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAAAAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGTCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTAGTCAAGTCATTAGATg                                                                                 >  2:807058/1‑150 (MQ=255)
                                                                   cATGCCAAAAGCGCCACACGCAATTGCCGAGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGAAGGATCAACTTGCTACTACTTTTTACTTGCACCTCATTAATACGGCAAGTCATTAGATGc                                                                                >  2:449367/1‑150 (MQ=255)
                                                                    aTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCt                                                                               >  1:89203/1‑150 (MQ=255)
                                                                         aaTAGCGCCAAAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAtt                                                                          <  2:866390/150‑1 (MQ=255)
                                                                          aTAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAttt                                                                         <  1:1105696/150‑1 (MQ=255)
                                                                           tAGCGCCACAGGCAATTGACGCGATTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCATCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAtttt                                                                        <  1:1307930/150‑1 (MQ=255)
                                                                            aGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTAGTTGCACCTCATTAATTCGGCAAGTGATTAGATGCTTGAttttt                                                                       <  2:1349447/150‑1 (MQ=255)
                                                                              cgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGa                                                                     >  2:736728/1‑150 (MQ=255)
                                                                               gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                    >  1:1427173/1‑150 (MQ=255)
                                                                                ccACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAg                                                                   >  2:541539/1‑150 (MQ=255)
                                                                                ccAAAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAg                                                                   <  2:1136890/150‑1 (MQ=255)
                                                                                 cacaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAgg                                                                  <  2:1312503/150‑1 (MQ=255)
                                                                                    aGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGtat                                                               <  2:1567062/150‑1 (MQ=255)
                                                                                     ggCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTTTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTACTTTTTACTTGCACATCATTAATTCGGCAAGTCATTAGATGCTAGATTTTTGAAGGtata                                                              >  1:245383/1‑150 (MQ=255)
                                                                                         aTTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTTAAGGTATACaaa                                                          >  1:715196/1‑150 (MQ=255)
                                                                                                cgAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCt                                                   <  2:160654/150‑1 (MQ=255)
                                                                                                  aGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGc                                                 <  1:1488352/150‑1 (MQ=255)
                                                                                                     ataAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCggg                                              <  2:1305674/150‑1 (MQ=255)
                                                                                                        aGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                           >  2:1171927/1‑150 (MQ=255)
                                                                                                        aGACGTATAATAAATTCTGCTATCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                           >  1:288492/1‑150 (MQ=255)
                                                                                                           cGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAAGTTGCTACTCCTTTTTACTTGCACCACATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttttta                                        >  2:712088/1‑149 (MQ=255)
                                                                                                           cGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTc                                        >  2:705969/1‑150 (MQ=255)
                                                                                                            gTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCt                                       <  1:171099/150‑1 (MQ=255)
                                                                                                            gTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATAAAAATTTTTCTGCGGGTTTTTCt                                       >  2:93779/1‑150 (MQ=255)
                                                                                                             tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                      >  2:969211/1‑150 (MQ=255)
                                                                                                              ataataAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTa                                     >  2:1235045/1‑150 (MQ=255)
                                                                                                               taataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTAc                                    <  2:398013/150‑1 (MQ=255)
                                                                                                                aataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACa                                   >  1:450482/1‑150 (MQ=255)
                                                                                                                 ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                  <  2:1733382/150‑1 (MQ=255)
                                                                                                                 ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                  >  1:534004/1‑150 (MQ=255)
                                                                                                                 ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGACAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                  >  1:784412/1‑150 (MQ=255)
                                                                                                                  taaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGt                                 <  2:1436442/150‑1 (MQ=255)
                                                                                                                         tgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGAt                          <  2:34042/150‑1 (MQ=255)
                                                                                                                           ctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCa                        >  2:410736/1‑150 (MQ=255)
                                                                                                                              tCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACtt                     <  1:310399/150‑1 (MQ=255)
                                                                                                                               cATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACttt                    <  2:610958/150‑1 (MQ=255)
                                                                                                                                atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTa                   >  1:541352/1‑150 (MQ=255)
                                                                                                                                atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTa                   >  1:353389/1‑150 (MQ=255)
                                                                                                                                atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTAAACTTGCTACTCCTTTTTCCTTGCACCTCATTAATTCGTCAAGTCATTAGAGGCTTGATTTTTGTAGGTATAAAAATTTTTCTGAGTGTTTTTCTTAAATTATTCTATCACtttt                   >  2:273832/1‑149 (MQ=255)
                                                                                                                                 tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAg                  <  2:1004828/150‑1 (MQ=255)
                                                                                                                                 tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCGACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAg                  <  1:78567/150‑1 (MQ=255)
                                                                                                                                    aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGTTATACAAATTATTCTGCGGGTTTTTCTTACAGTATTCGATAACTTTAGGaa               >  2:1681224/1‑150 (MQ=255)
                                                                                                                                          gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgat         >  1:342294/1‑149 (MQ=255)
                                                                                                                                          gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgag         >  1:422897/1‑150 (MQ=255)
                                                                                                                                          gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgag         >  1:1704247/1‑150 (MQ=255)
                                                                                                                                           cAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAGAAAGTCGGCAAGTCATTAGCAGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCTCTTTAGGAATTtgagt        >  1:234038/1‑150 (MQ=255)
                                                                                                                                            aaaaaGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGAGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg       >  2:355203/1‑150 (MQ=38)
                                                                                                                                             aaaaGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga      >  1:289291/1‑150 (MQ=38)
                                                                                                                                             aaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga      <  2:791768/150‑1 (MQ=255)
                                                                                                                                             aaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCATTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga      >  1:1108035/1‑150 (MQ=255)
                                                                                                                                               aaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTgaga    <  2:164335/150‑1 (MQ=255)
                                                                                                                                                aGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGAt   >  2:441612/1‑150 (MQ=39)
                                                                                                                                                 ggAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACATCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATc  >  2:1325274/1‑150 (MQ=38)
                                                                                                                                                     |                                                                                                                                                 
GGGCGCTTCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATC  >  NC_000913/3655773‑3656067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: