Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,655,929 A→T 5.8% intergenic (‑27/+37) yhiD ← / ← hdeB putative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,9290AT 5.8% 440.4 / 10.0 148intergenic (‑27/+37)yhiD/hdeBputative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (77/62);  new base T (4/5);  total (81/67)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

TCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTA  >  NC_000913/3655780‑3656078
                                                                                                                                                     |                                                                                                                                                     
  ccATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGGCTTTCCATGCCAATAGCGCCACAGGCAATTGACGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATg                                                                                                                                                     <  2:737044/150‑1 (MQ=255)
    aTGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGc                                                                                                                                                   <  1:1425920/150‑1 (MQ=255)
    aTGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGACAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGc                                                                                                                                                   >  1:819367/1‑150 (MQ=255)
       ccAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCa                                                                                                                                                <  1:854462/150‑1 (MQ=255)
         aaTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCAAATAGCGCCACAGGCAATTGCCGCGAGTATAAGAAGTATATTAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAAc                                                                                                                                              >  1:80791/1‑150 (MQ=255)
          aTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATATTAAAATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACt                                                                                                                                             >  1:449736/1‑150 (MQ=255)
             aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                                          >  1:1008689/1‑150 (MQ=255)
             aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                                          >  1:416551/1‑150 (MQ=255)
             aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                                          >  1:601000/1‑150 (MQ=255)
              aTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGGATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCt                                                                                                                                         <  2:1500865/150‑1 (MQ=255)
               aaCATGGGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCCTTCCCTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATACTAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGTTGCGCCATCTTGCtg                                                                                                                                        <  1:308747/149‑2 (MQ=255)
                 cATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACt                                                                                                                                      >  2:723285/1‑150 (MQ=255)
                   tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                                    <  2:1356850/150‑1 (MQ=39)
                    ttgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGTAAATTGCCGCGAGTATAAGACGTATAATAAATTCTTCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCt                                                                                                                                   <  1:1001279/149‑1 (MQ=255)
                          gTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCtttttgc                                                                                                                             <  1:1500065/150‑3 (MQ=38)
                          gTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGATGGCTCAACTTGCTACTCCTTTTTAc                                                                                                                             <  2:1394219/150‑1 (MQ=255)
                            aaCCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACtt                                                                                                                           <  1:631348/150‑1 (MQ=255)
                              cccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGc                                                                                                                         >  2:1638511/1‑150 (MQ=255)
                              cccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGc                                                                                                                         <  2:1436492/150‑1 (MQ=255)
                               ccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCTCAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCATGATGGCTCAACTTGATACTCCTTTTTACTTTCa                                                                                                                        >  1:1222635/1‑150 (MQ=255)
                                cTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCACCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAc                                                                                                                       >  1:1343993/1‑150 (MQ=255)
                                      cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAACGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                                                                                                 <  1:1068701/150‑1 (MQ=255)
                                      cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                                                                                                 >  2:171099/1‑150 (MQ=255)
                                       tttGCCGCGCATTTGCCTTTCCATGCCAATATCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGGCATATAAAAAAGCAAAAAGGATCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                                                                                                <  1:157940/150‑1 (MQ=255)
                                       tttGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                                                                                                <  1:1056897/150‑1 (MQ=255)
                                        ttGCCGCGCATTTGACTTTCCATGCCAATAGCTCCACATGCAATTGCCGCGAGTATAAGACGTTTAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTaa                                                                                                               <  2:474812/150‑1 (MQ=255)
                                          gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                             >  2:479791/1‑150 (MQ=255)
                                          gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                             >  1:1414215/1‑150 (MQ=255)
                                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTc                                                                                                                                                 >  2:1137895/1‑112 (MQ=255)
                                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTc                                                                                                                                                 <  1:1137895/112‑1 (MQ=255)
                                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                           <  1:70555/150‑1 (MQ=255)
                                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                           >  1:1355353/1‑150 (MQ=255)
                                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTATTTACTTGCACCTCATTAAttag                                                                                                           >  1:229402/1‑148 (MQ=255)
                                                    tGCCTTTCCATGCCAATAGCGCCCCAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCAGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTTCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGACa                                                                                                   >  1:933868/1‑150 (MQ=255)
                                                      ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCAtt                                                                                                 <  2:1008689/150‑1 (MQ=255)
                                                       cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                                >  2:1412964/1‑150 (MQ=255)
                                                         ttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTatt                                                                                              >  2:771383/1‑148 (MQ=255)
                                                           ccATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAAAAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGTCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTAGTCAAGTCATTAGATg                                                                                            >  2:807058/1‑150 (MQ=255)
                                                            cATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGc                                                                                           >  2:310399/1‑150 (MQ=255)
                                                             aTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCt                                                                                          >  1:89203/1‑150 (MQ=255)
                                                              tGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCtt                                                                                         >  1:398013/1‑150 (MQ=255)
                                                              cggcAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCtt                                                                                         >  1:1083105/4‑150 (MQ=255)
                                                                  aaTAGCGCCAAAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAtt                                                                                     <  2:866390/150‑1 (MQ=255)
                                                                   aTAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAttt                                                                                    <  1:1105696/150‑1 (MQ=255)
                                                                    tAGCGCCACAGGCAATTGACGCGATTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCATCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAtttt                                                                                   <  1:1307930/150‑1 (MQ=255)
                                                                      gagcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGTCAAGTCATTAGATGCTTGATTTTTg                                                                                 <  2:1176439/148‑1 (MQ=255)
                                                                       cgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGa                                                                                <  2:1071043/150‑1 (MQ=255)
                                                                        gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                               >  1:1427173/1‑150 (MQ=255)
                                                                        gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                               >  1:480075/1‑150 (MQ=255)
                                                                        gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAAATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                               <  1:442053/150‑1 (MQ=255)
                                                                         ccAAAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAg                                                                              <  2:1136890/150‑1 (MQ=255)
                                                                          cacaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAgg                                                                             >  2:1419014/1‑150 (MQ=255)
                                                                               gaaaTTGCATCGAGTTTAAGACTTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGTTATAc                                                                        <  1:314530/148‑1 (MQ=255)
                                                                               gCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATAc                                                                        >  1:278420/1‑150 (MQ=255)
                                                                                  aTTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTTAAGGTATACaaa                                                                     >  1:715196/1‑150 (MQ=255)
                                                                                        gcgAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTc                                                               <  2:1459772/150‑1 (MQ=255)
                                                                                         cgAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCt                                                              <  2:160654/150‑1 (MQ=255)
                                                                                              ataAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCggg                                                         <  2:1305674/150‑1 (MQ=255)
                                                                                                 aGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                                      >  2:1171927/1‑150 (MQ=255)
                                                                                                 aGACGTATAATAAATTCTGCTATCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                                      >  1:288492/1‑150 (MQ=255)
                                                                                                    cGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAAGTTGCTACTCCTTTTTACTTGCACCACATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttttta                                                   >  2:712088/1‑149 (MQ=255)
                                                                                                     gTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCt                                                  <  1:171099/150‑1 (MQ=255)
                                                                                                      tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                 >  2:969211/1‑150 (MQ=255)
                                                                                                      tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                 <  2:914657/150‑1 (MQ=255)
                                                                                                      tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                 <  2:2631/150‑1 (MQ=255)
                                                                                                       ataataAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTa                                                >  1:1310001/1‑150 (MQ=255)
                                                                                                        taataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTAc                                               <  2:398013/150‑1 (MQ=255)
                                                                                                          ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                             >  1:534004/1‑150 (MQ=255)
                                                                                                          ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                             <  2:1733382/150‑1 (MQ=255)
                                                                                                          ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGACAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                             >  1:784412/1‑150 (MQ=255)
                                                                                                              aTTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTATTTACTTGCACCTCATTAATTCGGCAAGGCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTAtt                                         <  2:1542898/150‑1 (MQ=255)
                                                                                                               ttCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTc                                        <  2:1291433/150‑1 (MQ=255)
                                                                                                                  tgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGAt                                     <  2:34042/150‑1 (MQ=255)
                                                                                                                   gctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATc                                    <  1:1297392/150‑1 (MQ=255)
                                                                                                                    ctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCa                                   <  2:1690252/150‑1 (MQ=255)
                                                                                                                    ctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCa                                   >  2:410736/1‑150 (MQ=255)
                                                                                                                      gTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACt                                 >  2:375615/1‑150 (MQ=255)
                                                                                                                        cATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACttt                               <  2:610958/150‑1 (MQ=255)
                                                                                                                         atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTa                              >  1:541352/1‑150 (MQ=255)
                                                                                                                         atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTAAACTTGCTACTCCTTTTTCCTTGCACCTCATTAATTCGTCAAGTCATTAGAGGCTTGATTTTTGTAGGTATAAAAATTTTTCTGAGTGTTTTTCTTAAATTATTCTATCACtttt                              >  2:273832/1‑149 (MQ=255)
                                                                                                                          tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCGACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAg                             <  1:78567/150‑1 (MQ=255)
                                                                                                                             aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGTTATACAAATTATTCTGCGGGTTTTTCTTACAGTATTCGATAACTTTAGGaa                          >  2:1681224/1‑150 (MQ=255)
                                                                                                                             aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGaa                          >  2:1325146/1‑150 (MQ=255)
                                                                                                                             aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATCCTTGATTTTTGAAGGTAAACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGTaa                          >  2:640523/1‑150 (MQ=255)
                                                                                                                                  tGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtga                     >  2:811841/1‑150 (MQ=255)
                                                                                                                                   gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgat                    >  1:342294/1‑149 (MQ=255)
                                                                                                                                   gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgag                    >  1:1704247/1‑150 (MQ=255)
                                                                                                                                    cAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAGAAAGTCGGCAAGTCATTAGCAGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCTCTTTAGGAATTtgagt                   >  1:234038/1‑150 (MQ=255)
                                                                                                                                     aaaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg                  <  2:525474/150‑1 (MQ=255)
                                                                                                                                     aaaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg                  >  1:1560283/1‑150 (MQ=255)
                                                                                                                                        aagaagTAGCAAGATGAGACATATTGCTGCTCCTTTTTACTTGCACCTAATTAATTCGGAAAGTCATTTGATTCTTGATTTTTGAAGGTATACAAATTTTTCTGAGTGTTTTTCTTACAGTATTAGATCACTTTAGTAATTTGATTgaga               >  1:292548/1‑150 (MQ=255)
                                                                                                                                         aGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGAt              >  2:441612/1‑150 (MQ=39)
                                                                                                                                          ggAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACATCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATc             >  2:1325274/1‑150 (MQ=38)
                                                                                                                                               agcaAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGttt        <  1:482323/150‑1 (MQ=255)
                                                                                                                                                   aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt    <  2:952892/150‑1 (MQ=255)
                                                                                                                                                   aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt    <  1:1275209/150‑1 (MQ=255)
                                                                                                                                                   aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt    <  2:539240/150‑1 (MQ=255)
                                                                                                                                                   aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGGCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt    >  1:132172/1‑150 (MQ=255)
                                                                                                                                                    gATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAttt   <  1:996961/150‑1 (MQ=255)
                                                                                                                                                     aTGGCTCAACTTTCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTa  <  1:1665943/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTA  >  NC_000913/3655780‑3656078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: