Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,655,934 T→C 6.0% intergenic (‑32/+32) yhiD ← / ← hdeB putative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,9340TC 6.0% 445.0 / 10.6 150intergenic (‑32/+32)yhiD/hdeBputative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein
Reads supporting (aligned to +/- strand):  ref base T (75/65);  new base C (4/5);  total (80/70)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 150 total aligned reads displayed.

CCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAG  >  NC_000913/3655787‑3656081
                                                                                                                                                   |                                                                                                                                                   
ccAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCa                                                                                                                                                   <  1:854462/150‑1 (MQ=255)
 cAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCaa                                                                                                                                                  >  2:1164619/1‑150 (MQ=255)
  aaTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCAAATAGCGCCACAGGCAATTGCCGCGAGTATAAGAAGTATATTAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAAc                                                                                                                                                 >  1:80791/1‑150 (MQ=255)
   aTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATATTAAAATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACt                                                                                                                                                >  1:449736/1‑150 (MQ=255)
     tAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTg                                                                                                                                              >  1:1567062/1‑150 (MQ=255)
      aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                                             >  1:1008689/1‑150 (MQ=255)
      aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                                             >  1:416551/1‑150 (MQ=255)
       aTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGGATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCt                                                                                                                                            <  2:1500865/150‑1 (MQ=255)
        aaCATGGGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCCTTCCCTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATACTAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGTTGCGCCATCTTGCtg                                                                                                                                           <  1:308747/149‑2 (MQ=255)
          cATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACt                                                                                                                                         >  2:723285/1‑150 (MQ=255)
            tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                                       <  2:140190/150‑1 (MQ=39)
            tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                                       <  2:1356850/150‑1 (MQ=39)
             ttgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGTAAATTGCCGCGAGTATAAGACGTATAATAAATTCTTCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCt                                                                                                                                      <  1:1001279/149‑1 (MQ=255)
                     aaCCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACtt                                                                                                                              <  1:631348/150‑1 (MQ=255)
                       cccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGc                                                                                                                            <  2:1436492/150‑1 (MQ=255)
                        ccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCTCAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCATGATGGCTCAACTTGATACTCCTTTTTACTTTCa                                                                                                                           >  1:1222635/1‑150 (MQ=255)
                         cTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCACCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAc                                                                                                                          >  1:1343993/1‑150 (MQ=255)
                          tGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAcc                                                                                                                         >  1:761988/1‑150 (MQ=255)
                             tCCTTTGCCGCGCTTTTGCCTTTCCATGCCAATTGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATTATAAATTGTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCa                                                                                                                      <  1:593340/150‑1 (MQ=255)
                               cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAACGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                                                                                                    <  1:1068701/150‑1 (MQ=255)
                                tttGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                                                                                                   <  1:1056897/150‑1 (MQ=255)
                                   gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                                >  1:1414215/1‑150 (MQ=255)
                                   gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                                >  2:479791/1‑150 (MQ=255)
                                   gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                                >  2:1488352/1‑150 (MQ=255)
                                    ccGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTc                                                                                                               <  2:51394/150‑1 (MQ=255)
                                     ctcgcATATGCCTTTCAATGTCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGCCGTATAATAAATTCTGATGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                              <  2:292996/148‑1 (MQ=255)
                                     cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTc                                                                                                                                                    <  1:1137895/112‑1 (MQ=255)
                                     cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                              >  1:64633/1‑150 (MQ=255)
                                     cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                              >  1:1355353/1‑150 (MQ=255)
                                     cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTATTTACTTGCACCTCATTAAttag                                                                                                              >  1:229402/1‑148 (MQ=255)
                                          ctttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACCTATAATAACATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCCAGATGGCTCAACTCGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                                                                                         <  2:859830/149‑1 (MQ=255)
                                             tGCCTTTCCATGCCAATAGCGCCCCAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCAGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTTCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGACa                                                                                                      >  1:933868/1‑150 (MQ=255)
                                              gCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTAGCACCTCATTAATTCGGCAAGTCAt                                                                                                     >  2:1071377/1‑150 (MQ=255)
                                               ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCAtt                                                                                                    <  2:1008689/150‑1 (MQ=255)
                                                cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                                   >  2:201236/1‑150 (MQ=255)
                                                cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                                   >  2:1412964/1‑150 (MQ=255)
                                                  ttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTatt                                                                                                 >  2:771383/1‑148 (MQ=255)
                                                     cATGCCAAAAGCGCCACACGCAATTGCCGAGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGAAGGATCAACTTGCTACTACTTTTTACTTGCACCTCATTAATACGGCAAGTCATTAGATGc                                                                                              >  2:449367/1‑150 (MQ=255)
                                                      aTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCt                                                                                             >  1:89203/1‑150 (MQ=255)
                                                       tGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCtt                                                                                            >  1:398013/1‑150 (MQ=255)
                                                       cggcAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCtt                                                                                            >  1:1083105/4‑150 (MQ=255)
                                                        gCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTg                                                                                           <  2:416551/150‑1 (MQ=255)
                                                            aTAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAttt                                                                                       <  1:1105696/150‑1 (MQ=255)
                                                             tAGCGCCACAGGCAATTGACGCGATTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCATCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAtttt                                                                                      <  1:1307930/150‑1 (MQ=255)
                                                              aGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTAGTTGCACCTCATTAATTCGGCAAGTGATTAGATGCTTGAttttt                                                                                     <  2:1349447/150‑1 (MQ=255)
                                                                cgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGa                                                                                   >  2:736728/1‑150 (MQ=255)
                                                                 gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                                  >  1:1427173/1‑150 (MQ=255)
                                                                 gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                                  >  1:480075/1‑150 (MQ=255)
                                                                 gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAAATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                                  <  1:442053/150‑1 (MQ=255)
                                                                  ccACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAg                                                                                 >  2:541539/1‑150 (MQ=255)
                                                                  ccAAAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAg                                                                                 <  2:1136890/150‑1 (MQ=255)
                                                                   cacaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAgg                                                                                <  2:1312503/150‑1 (MQ=255)
                                                                      aGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGtat                                                                             <  2:1567062/150‑1 (MQ=255)
                                                                        gaaaTTGCATCGAGTTTAAGACTTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGTTATAc                                                                           <  1:314530/148‑1 (MQ=255)
                                                                        gCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATAc                                                                           >  1:278420/1‑150 (MQ=255)
                                                                           aTTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTTAAGGTATACaaa                                                                        >  1:715196/1‑150 (MQ=255)
                                                                                  cgAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCt                                                                 <  2:160654/150‑1 (MQ=255)
                                                                                    aGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGc                                                               <  1:1488352/150‑1 (MQ=255)
                                                                                          aGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                                         >  2:1171927/1‑150 (MQ=255)
                                                                                          aGACGTATAATAAATTCTGCTATCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                                         >  1:288492/1‑150 (MQ=255)
                                                                                             cGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTc                                                      >  2:705969/1‑150 (MQ=255)
                                                                                              gTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCt                                                     <  1:171099/150‑1 (MQ=255)
                                                                                               tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                    <  2:914657/150‑1 (MQ=255)
                                                                                               tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                    >  2:969211/1‑150 (MQ=255)
                                                                                                ataataAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTa                                                   >  2:1235045/1‑150 (MQ=255)
                                                                                                ataataAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTa                                                   >  1:1310001/1‑150 (MQ=255)
                                                                                                 taataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTAc                                                  <  2:398013/150‑1 (MQ=255)
                                                                                                   ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                                <  2:1733382/150‑1 (MQ=255)
                                                                                                    taaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGt                                               <  2:1436442/150‑1 (MQ=255)
                                                                                                        ttCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTc                                           <  2:1291433/150‑1 (MQ=255)
                                                                                                           tgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGAt                                        <  2:34042/150‑1 (MQ=255)
                                                                                                            gctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATc                                       <  1:1297392/150‑1 (MQ=255)
                                                                                                             ctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCa                                      >  2:410736/1‑150 (MQ=255)
                                                                                                             ctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCa                                      <  2:1690252/150‑1 (MQ=255)
                                                                                                                  atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTa                                 >  1:541352/1‑150 (MQ=255)
                                                                                                                  atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTAAACTTGCTACTCCTTTTTCCTTGCACCTCATTAATTCGTCAAGTCATTAGAGGCTTGATTTTTGTAGGTATAAAAATTTTTCTGAGTGTTTTTCTTAAATTATTCTATCACtttt                                 >  2:273832/1‑149 (MQ=255)
                                                                                                                   tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAg                                <  2:1004828/150‑1 (MQ=255)
                                                                                                                   tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCGACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAg                                <  1:78567/150‑1 (MQ=255)
                                                                                                                      aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGTTATACAAATTATTCTGCGGGTTTTTCTTACAGTATTCGATAACTTTAGGaa                             >  2:1681224/1‑150 (MQ=255)
                                                                                                                      aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGaa                             >  2:1325146/1‑150 (MQ=255)
                                                                                                                      aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATCCTTGATTTTTGAAGGTAAACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGTaa                             >  2:640523/1‑150 (MQ=255)
                                                                                                                           tGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtga                        >  2:811841/1‑150 (MQ=255)
                                                                                                                            gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgat                       >  1:342294/1‑149 (MQ=255)
                                                                                                                            gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgag                       >  1:1704247/1‑150 (MQ=255)
                                                                                                                             cAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAGAAAGTCGGCAAGTCATTAGCAGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCTCTTTAGGAATTtgagt                      >  1:234038/1‑150 (MQ=255)
                                                                                                                              aaaaaGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGAGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg                     >  2:355203/1‑150 (MQ=38)
                                                                                                                              aaaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg                     >  1:1560283/1‑150 (MQ=255)
                                                                                                                               aaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga                    <  2:791768/150‑1 (MQ=255)
                                                                                                                                 aagaagTAGCAAGATGAGACATATTGCTGCTCCTTTTTACTTGCACCTAATTAATTCGGAAAGTCATTTGATTCTTGATTTTTGAAGGTATACAAATTTTTCTGAGTGTTTTTCTTACAGTATTAGATCACTTTAGTAATTTGATTgaga                  >  1:292548/1‑150 (MQ=255)
                                                                                                                                 aaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTgaga                  <  2:164335/150‑1 (MQ=255)
                                                                                                                                        agcaAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGttt           <  1:482323/150‑1 (MQ=255)
                                                                                                                                          caAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCa         >  2:1692017/1‑150 (MQ=255)
                                                                                                                                            aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt       <  2:539240/150‑1 (MQ=255)
                                                                                                                                            aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt       <  2:952892/150‑1 (MQ=255)
                                                                                                                                            aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt       <  1:1275209/150‑1 (MQ=255)
                                                                                                                                            aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGGCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt       >  1:132172/1‑150 (MQ=255)
                                                                                                                                             gATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAttt      <  1:996961/150‑1 (MQ=255)
                                                                                                                                              aTGGCTCAACTTTCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTa     <  1:1665943/150‑1 (MQ=255)
                                                                                                                                                 gCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAg  <  1:1638511/150‑1 (MQ=255)
                                                                                                                                                 gCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAg  <  2:897573/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAG  >  NC_000913/3655787‑3656081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: