Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,655,937 A→T 6.8% intergenic (‑35/+29) yhiD ← / ← hdeB putative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,9370AT 6.8% 417.7 / 13.2 147intergenic (‑35/+29)yhiD/hdeBputative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (74/63);  new base T (5/5);  total (79/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 149 total aligned reads displayed.

CAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACG  >  NC_000913/3655788‑3656086
                                                                                                                                                     |                                                                                                                                                     
cAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCaa                                                                                                                                                       >  2:1164619/1‑150 (MQ=255)
 aaTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCAAATAGCGCCACAGGCAATTGCCGCGAGTATAAGAAGTATATTAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAAc                                                                                                                                                      >  1:80791/1‑150 (MQ=255)
  aTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATATTAAAATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACt                                                                                                                                                     >  1:449736/1‑150 (MQ=255)
  aTTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACt                                                                                                                                                     >  1:1319876/1‑150 (MQ=255)
    tAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTg                                                                                                                                                   >  1:1567062/1‑150 (MQ=255)
     aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                                                  >  1:1008689/1‑150 (MQ=255)
     aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                                                  >  1:601000/1‑150 (MQ=255)
     aaTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGc                                                                                                                                                  >  1:416551/1‑150 (MQ=255)
      aTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGGATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCt                                                                                                                                                 <  2:1500865/150‑1 (MQ=255)
       aaCATGGGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCCTTCCCTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATACTAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGTTGCGCCATCTTGCtg                                                                                                                                                <  1:308747/149‑2 (MQ=255)
        aCATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTTCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTAc                                                                                                                                               <  1:1305387/150‑1 (MQ=255)
         cATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACt                                                                                                                                              >  2:723285/1‑150 (MQ=255)
           tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                                            <  2:1356850/150‑1 (MQ=39)
           tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                                            <  2:140190/150‑1 (MQ=39)
            ttgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGTAAATTGCCGCGAGTATAAGACGTATAATAAATTCTTCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCt                                                                                                                                           <  1:1001279/149‑1 (MQ=255)
             tgtgCGTAACCCTGCTCCATTGCCGCGCATTTGCCTATCCATGCGAATAGCGCCACTGGCATTTGCCGCGAGTATAAGACGTCTAATAAATTCTGCTGTCATATAAAACTGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGATGCTCCtt                                                                                                                                          <  1:1068863/150‑1 (MQ=255)
                gCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCttttt                                                                                                                                       >  1:1733382/1‑150 (MQ=255)
                gCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCttttt                                                                                                                                       >  1:1265598/1‑150 (MQ=255)
                  gTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCtttttgc                                                                                                                                     <  1:1500065/150‑3 (MQ=38)
                      cccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGc                                                                                                                                 <  2:1436492/150‑1 (MQ=255)
                       ccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCTCAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCATGATGGCTCAACTTGATACTCCTTTTTACTTTCa                                                                                                                                >  1:1222635/1‑150 (MQ=255)
                        cTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTCTCATATAAAAATGCAAAAAGTAGCAGCAAGAGGGCTCAACTTGCTACTCCTTTTTACTTGCAc                                                                                                                               <  1:852242/150‑1 (MQ=255)
                        cTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCACCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAc                                                                                                                               >  1:1343993/1‑150 (MQ=255)
                         tGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAcc                                                                                                                              >  1:761988/1‑150 (MQ=255)
                            tCCTTTGCCGCGCTTTTGCCTTTCCATGCCAATTGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATTATAAATTGTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCa                                                                                                                           <  1:593340/150‑1 (MQ=255)
                              cTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAACGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAtt                                                                                                                         <  1:1068701/150‑1 (MQ=255)
                               tttGCCGCGCATTTGCCTTTCCATGCCAATATCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGGCATATAAAAAAGCAAAAAGGATCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                                                                                                        <  1:157940/150‑1 (MQ=255)
                                  gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                                     >  2:1488352/1‑150 (MQ=255)
                                  gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                                     >  2:479791/1‑150 (MQ=255)
                                  gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                                     >  1:1414215/1‑150 (MQ=255)
                                   ccGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTc                                                                                                                    <  2:51394/150‑1 (MQ=255)
                                    ctcgcATATGCCTTTCAATGTCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGCCGTATAATAAATTCTGATGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                   <  2:292996/148‑1 (MQ=255)
                                    cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATTCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                   <  1:1418039/150‑1 (MQ=255)
                                    cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                   >  1:1689480/1‑150 (MQ=255)
                                    cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                   >  1:1349447/1‑150 (MQ=255)
                                    cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                   >  1:64633/1‑150 (MQ=255)
                                    cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                   <  1:70555/150‑1 (MQ=255)
                                      cgcATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGc                                                                                                                 <  1:1028545/150‑1 (MQ=255)
                                         ctttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACCTATAATAACATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCCAGATGGCTCAACTCGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                                                                                              <  2:859830/149‑1 (MQ=255)
                                            tGCCTTTCCATGCCAATAGCGCCCCAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCAGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTTCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGACa                                                                                                           >  1:933868/1‑150 (MQ=255)
                                             gCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTAGCACCTCATTAATTCGGCAAGTCAt                                                                                                          >  2:1071377/1‑150 (MQ=255)
                                              ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCAtt                                                                                                         <  2:1008689/150‑1 (MQ=255)
                                               cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                                        >  2:201236/1‑150 (MQ=255)
                                               cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                                        >  2:1412964/1‑150 (MQ=255)
                                                 ttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTatt                                                                                                      >  2:771383/1‑148 (MQ=255)
                                                    cATGCCAAAAGCGCCACACGCAATTGCCGAGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGAAGGATCAACTTGCTACTACTTTTTACTTGCACCTCATTAATACGGCAAGTCATTAGATGc                                                                                                   >  2:449367/1‑150 (MQ=255)
                                                           aTAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAttt                                                                                            <  1:1105696/150‑1 (MQ=255)
                                                             aGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTAGTTGCACCTCATTAATTCGGCAAGTGATTAGATGCTTGAttttt                                                                                          <  2:1349447/150‑1 (MQ=255)
                                                               cgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGa                                                                                        <  2:1071043/150‑1 (MQ=255)
                                                               cgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGa                                                                                        >  2:736728/1‑150 (MQ=255)
                                                                 ccACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAg                                                                                      >  2:541539/1‑150 (MQ=255)
                                                                  cacaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAgg                                                                                     <  2:1312503/150‑1 (MQ=255)
                                                                     aGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGtat                                                                                  <  2:1567062/150‑1 (MQ=255)
                                                                      ggCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTTTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTACTTTTTACTTGCACATCATTAATTCGGCAAGTCATTAGATGCTAGATTTTTGAAGGtata                                                                                 >  1:245383/1‑150 (MQ=255)
                                                                                 cgAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCt                                                                      <  2:160654/150‑1 (MQ=255)
                                                                                   aGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGc                                                                    <  1:1488352/150‑1 (MQ=255)
                                                                                         aGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                                              >  2:1171927/1‑150 (MQ=255)
                                                                                         aGACGTATAATAAATTCTGCTATCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                                              >  1:288492/1‑150 (MQ=255)
                                                                                            cGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTc                                                           >  2:705969/1‑150 (MQ=255)
                                                                                             gTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCt                                                          <  1:171099/150‑1 (MQ=255)
                                                                                              tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                         <  2:914657/150‑1 (MQ=255)
                                                                                              tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                         >  2:969211/1‑150 (MQ=255)
                                                                                               ataataAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTa                                                        >  2:1235045/1‑150 (MQ=255)
                                                                                               ataataAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTa                                                        >  1:1310001/1‑150 (MQ=255)
                                                                                                taataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTAc                                                       <  2:398013/150‑1 (MQ=255)
                                                                                                 aataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACa                                                      >  1:450482/1‑150 (MQ=255)
                                                                                                  ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                                     >  1:534004/1‑150 (MQ=255)
                                                                                                  ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                                     <  2:1733382/150‑1 (MQ=255)
                                                                                                  ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGACAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                                     >  1:784412/1‑150 (MQ=255)
                                                                                                   taaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGt                                                    <  2:1436442/150‑1 (MQ=255)
                                                                                                       ttCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTc                                                <  2:1291433/150‑1 (MQ=255)
                                                                                                          tgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGAt                                             <  2:34042/150‑1 (MQ=255)
                                                                                                           gctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATc                                            <  1:1297392/150‑1 (MQ=255)
                                                                                                            ctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCa                                           >  2:410736/1‑150 (MQ=255)
                                                                                                            ctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCa                                           <  2:1690252/150‑1 (MQ=255)
                                                                                                               tCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACtt                                        <  1:310399/150‑1 (MQ=255)
                                                                                                                 atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTa                                      >  1:353389/1‑150 (MQ=255)
                                                                                                                 atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTAAACTTGCTACTCCTTTTTCCTTGCACCTCATTAATTCGTCAAGTCATTAGAGGCTTGATTTTTGTAGGTATAAAAATTTTTCTGAGTGTTTTTCTTAAATTATTCTATCACtttt                                      >  2:273832/1‑149 (MQ=255)
                                                                                                                  tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAg                                     <  2:1004828/150‑1 (MQ=255)
                                                                                                                     aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGTTATACAAATTATTCTGCGGGTTTTTCTTACAGTATTCGATAACTTTAGGaa                                  >  2:1681224/1‑150 (MQ=255)
                                                                                                                     aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGaa                                  >  2:1325146/1‑150 (MQ=255)
                                                                                                                     aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATCCTTGATTTTTGAAGGTAAACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGTaa                                  >  2:640523/1‑150 (MQ=255)
                                                                                                                          tGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtga                             >  2:811841/1‑150 (MQ=255)
                                                                                                                           gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgat                            >  1:342294/1‑149 (MQ=255)
                                                                                                                           gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgag                            >  1:422897/1‑150 (MQ=255)
                                                                                                                            cAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCAGAAAGTCGGCAAGTCATTAGCAGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCTCTTTAGGAATTtgagt                           >  1:234038/1‑150 (MQ=255)
                                                                                                                             aaaaaGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGAGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg                          >  2:355203/1‑150 (MQ=38)
                                                                                                                              aaaaGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga                         >  1:289291/1‑150 (MQ=38)
                                                                                                                              aaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga                         <  2:791768/150‑1 (MQ=255)
                                                                                                                              aaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCATTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga                         >  1:1108035/1‑150 (MQ=255)
                                                                                                                                aaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTgaga                       <  2:164335/150‑1 (MQ=255)
                                                                                                                                 aGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGAt                      >  2:441612/1‑150 (MQ=39)
                                                                                                                                       agcaAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGttt                <  1:482323/150‑1 (MQ=255)
                                                                                                                                        gcaAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTc               >  1:897573/1‑150 (MQ=255)
                                                                                                                                         caAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCa              >  2:1692017/1‑150 (MQ=255)
                                                                                                                                           aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt            <  2:539240/150‑1 (MQ=255)
                                                                                                                                           aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt            <  2:952892/150‑1 (MQ=255)
                                                                                                                                             aTGGCTCAACTTTCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTa          <  1:1665943/150‑1 (MQ=255)
                                                                                                                                                gCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAg       <  2:897573/150‑1 (MQ=255)
                                                                                                                                                gCTCAACTTGCTACTCCATTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTaaat       >  1:999520/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACG  >  NC_000913/3655788‑3656086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: