Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 464,356 A→T 5.5% intergenic (+48/‑46) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913464,3560AT 5.5% 312.1 / 5.0 108intergenic (+48/‑46)ybaV/fadMputative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
Reads supporting (aligned to +/- strand):  ref base A (55/47);  new base T (4/2);  total (59/49)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 110 total aligned reads displayed.

CGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGTT  >  NC_000913/464207‑464505
                                                                                                                                                      |                                                                                                                                                     
cgcgAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCa                                                                                                                                                       <  1:767393/150‑1 (MQ=255)
 gcgAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGAAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAg                                                                                                                                                      >  1:1605775/1‑150 (MQ=255)
     agagTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTAt                                                                                                                                                  >  1:534012/1‑150 (MQ=255)
        gTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATCGGCAATTCGCAGGTGGAACGTAAACTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAc                                                                                                                                               >  1:20889/1‑150 (MQ=255)
         tACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                                                                                                              >  2:1506730/1‑150 (MQ=255)
         tACGGGCCGTTTAACCCTGTGGAGGATCTACAGCAGGTGCCGGGGATGGGCAATTCGCTGGGGGAACGTAATCTGGCGGTAGTTACCCTGTAATTAATTTGCCTAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                                                                                                              <  1:97486/150‑1 (MQ=255)
          aCGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACct                                                                                                                                             <  2:1112988/150‑1 (MQ=255)
              tacGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGTGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAATAGGTCATAACTGGTATGACctcttc                                                                                                                                         <  2:159586/148‑3 (MQ=255)
               ccGTTTAAAACTGTGGAGGATCTAAAGCAGGTGACGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTa                                                                                                                                        >  1:603756/1‑150 (MQ=255)
                cGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                                                                       <  1:467929/150‑1 (MQ=255)
                cGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                                                                       <  1:978122/150‑1 (MQ=255)
                cGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                                                                       <  1:1336702/150‑1 (MQ=255)
                 gTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACt                                                                                                                                      >  1:348869/1‑150 (MQ=255)
                 gTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTAGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACt                                                                                                                                      >  1:892756/1‑150 (MQ=255)
                   ttAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTta                                                                                                                                    <  2:1384046/150‑1 (MQ=255)
                    tAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtat                                                                                                                                   <  1:513571/150‑1 (MQ=255)
                     aaaaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtata                                                                                                                                  <  1:546654/150‑1 (MQ=255)
                     aaaaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtata                                                                                                                                  >  2:1068372/1‑150 (MQ=255)
                         cTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACTAGTTATGACCTCTGTACTTATaacaa                                                                                                                              >  2:721591/1‑150 (MQ=255)
                         cTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaa                                                                                                                              >  1:1538778/1‑150 (MQ=255)
                         cTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaa                                                                                                                              >  1:1275989/1‑150 (MQ=255)
                             ggaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaac                                                                                                                          >  1:540821/1‑150 (MQ=255)
                             ggaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaac                                                                                                                          >  1:1259235/1‑150 (MQ=255)
                             ggaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaaacac                                                                                                                          >  2:1335190/1‑146 (MQ=255)
                                ggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTa                                                                                                                       <  2:1574143/150‑1 (MQ=255)
                                  aTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAg                                                                                                                     >  2:1289266/1‑150 (MQ=255)
                                   tCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGAGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAgg                                                                                                                    >  1:1172131/1‑150 (MQ=255)
                                       aaGCAGGTGCCGGGGATGGGAAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTAt                                                                                                                <  2:1499443/150‑1 (MQ=255)
                                        aGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTAtt                                                                                                               >  2:1358787/1‑150 (MQ=255)
                                        aGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTAtt                                                                                                               >  1:1097319/1‑150 (MQ=255)
                                         gCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTg                                                                                                              <  2:577776/150‑1 (MQ=255)
                                         gCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTg                                                                                                              <  2:1669078/150‑1 (MQ=255)
                                          cAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTATTTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACTACGTAAGGTTATTgc                                                                                                             <  2:1104943/150‑1 (MQ=255)
                                             gTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCt                                                                                                          >  1:888424/1‑150 (MQ=255)
                                               gCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTAt                                                                                                        >  1:753529/1‑150 (MQ=255)
                                               gCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTAt                                                                                                        <  2:1352107/150‑1 (MQ=255)
                                                ccGGGGAGGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                                                                       <  1:1514119/150‑1 (MQ=255)
                                                 cGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGc                                                                                                      >  2:1451537/1‑150 (MQ=255)
                                                  ggggATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCa                                                                                                     >  1:1050200/1‑150 (MQ=255)
                                                   gggATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCaa                                                                                                    <  2:1482886/150‑1 (MQ=255)
                                                     gATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAac                                                                                                  <  1:1015760/150‑1 (MQ=255)
                                                     gATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAac                                                                                                  <  1:473774/150‑1 (MQ=255)
                                                      aTGGGCAATTCGCTGGTGGAACGTACTCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATATTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAaca                                                                                                 <  1:99747/150‑1 (MQ=255)
                                                       tGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAacac                                                                                                >  1:74395/1‑150 (MQ=255)
                                                          gCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACaaa                                                                                             >  1:770209/1‑150 (MQ=255)
                                                                cGCTGGTGGAAAGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAg                                                                                       <  2:360225/150‑1 (MQ=255)
                                                                  cTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGtt                                                                                     <  1:489369/150‑1 (MQ=255)
                                                                    ggtggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGCCTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCg                                                                                   >  1:732427/1‑150 (MQ=255)
                                                                     gtggAACGTAATCTGGCGGTATTAACCCTCTAATTAATTTGCATAGTGGCAAATTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTAGt                                                                                  >  1:1683870/1‑150 (MQ=255)
                                                                     gtggAACGGAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACTTAAGTTTATTGCGCTATGCAAACACAAATCAAAGTTCga                                                                                  >  1:101273/1‑149 (MQ=255)
                                                                      tggAACGTAATCTGGCGGTATAAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTg                                                                                 <  1:689899/150‑1 (MQ=255)
                                                                       ggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATGACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTAGTgt                                                                                >  1:303196/1‑149 (MQ=255)
                                                                       ggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTgg                                                                                >  2:769816/1‑150 (MQ=255)
                                                                       ggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTgg                                                                                >  2:1232305/1‑150 (MQ=255)
                                                                        gAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGa                                                                               <  2:624200/150‑1 (MQ=255)
                                                                        gAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGa                                                                               <  2:553658/150‑1 (MQ=255)
                                                                          aCGTAATCTGGCGGTATTACCCCTGTAATTAATTTGCATTGTGGCAATTTTTTGCCAGACTGAAGTGGTCATACCAGTTATGTCCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGata                                                                             <  1:1228693/150‑1 (MQ=255)
                                                                             tAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatca                                                                          <  1:545557/150‑1 (MQ=255)
                                                                              aaTCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACAGAATTCAAAGTTCGTGGATatcat                                                                         >  2:1234224/1‑150 (MQ=255)
                                                                               aTCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatcatc                                                                        >  2:222479/1‑150 (MQ=255)
                                                                                 cTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCAtctc                                                                      <  2:1120707/150‑1 (MQ=255)
                                                                                 cTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCAtctc                                                                      >  2:469271/1‑150 (MQ=255)
                                                                                 cTGGCGGTATTAAACCTGTAATTAATTTGCATAGTGGCAATTTTTTTCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCAtctc                                                                      <  1:1436237/150‑1 (MQ=255)
                                                                                  tGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCg                                                                     >  1:110096/1‑150 (MQ=255)
                                                                                     gggTATTTACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCAAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACg                                                                  <  2:261625/149‑1 (MQ=255)
                                                                                      ggTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGt                                                                 <  2:1605775/150‑1 (MQ=255)
                                                                                       gTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGtt                                                                <  1:1500709/150‑1 (MQ=255)
                                                                                        tATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGttt                                                               >  2:1499959/1‑150 (MQ=255)
                                                                                            aaCCCTGTAATTAATTTGCATAGTGGCAATTTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAcc                                                            >  2:1731653/1‑150 (MQ=255)
                                                                                            aaCCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCa                                                           <  2:275320/150‑1 (MQ=255)
                                                                                               ccTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCa                                                        <  2:1721579/150‑1 (MQ=255)
                                                                                                 tGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACg                                                      <  2:619170/150‑1 (MQ=255)
                                                                                                   taattaatTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTc                                                    <  1:717860/150‑1 (MQ=255)
                                                                                                     attaattTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACATCTGTACTTATAACAACAACGTAAGGTTATTGCGATATGCAATCACAAATAAAAGTTCGTGGATATCATCTAGCCGTTTACCAGCAAGTcaa                                                  >  2:1119941/1‑150 (MQ=255)
                                                                                                             gCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGccc                                          <  2:770209/150‑1 (MQ=255)
                                                                                                                tAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCt                                       >  1:250377/1‑150 (MQ=255)
                                                                                                                    ggCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCt                                   >  1:98998/1‑150 (MQ=255)
                                                                                                                     gCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCtt                                  <  2:250377/150‑1 (MQ=255)
                                                                                                                     gCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCtt                                  >  2:402992/1‑150 (MQ=255)
                                                                                                                      cAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTg                                 <  1:386498/150‑1 (MQ=255)
                                                                                                                           tttttGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAAtt                             >  1:1271938/1‑150 (MQ=255)
                                                                                                                              ttGCCAGACTGAAGAGGTCATTCCAGTTATGACCTCTGTACTTATAACAACAACGTAATGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTtct                          <  1:1100614/150‑1 (MQ=255)
                                                                                                                               tGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCTCTACCTTGAATTtctc                         >  1:1720928/1‑150 (MQ=255)
                                                                                                                               tGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTtctc                         <  1:411415/150‑1 (MQ=255)
                                                                                                                                  cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCgaa                      >  2:1644660/1‑150 (MQ=255)
                                                                                                                                    taCTGAAGAGGTCATAACAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCgaaga                    <  1:1035438/149‑1 (MQ=255)
                                                                                                                                      gtacagagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCAGCTACCTTGAATTTCTCGaagaag                  >  1:1168128/5‑150 (MQ=255)
                                                                                                                                      gtacagagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAAAACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAACAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGaagaag                  >  1:1162121/5‑150 (MQ=255)
                                                                                                                                      cTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGaagaag                  >  1:867758/1‑150 (MQ=255)
                                                                                                                                         cagagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGccc               >  2:1445082/2‑150 (MQ=255)
                                                                                                                                           gagGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGc             <  2:534012/150‑1 (MQ=255)
                                                                                                                                           gagGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGc             >  2:655863/1‑150 (MQ=255)
                                                                                                                                           gagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGc             >  2:1356396/1‑150 (MQ=255)
                                                                                                                                             tgTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTg           >  1:838864/2‑150 (MQ=255)
                                                                                                                                                cATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGa        <  2:732427/150‑1 (MQ=255)
                                                                                                                                                 aTACCAGTTATGACCTCTGTACTTATAACAACCACGGAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGCATTTCTCGAAGAAGCCCGCTGGGAt       >  2:1491398/1‑150 (MQ=255)
                                                                                                                                                    ccAGTTATGACCTCTGTACTTATAACAACAACGTTAGGTTATTGAGCTAAGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATtgg    <  2:603756/150‑1 (MQ=255)
                                                                                                                                                    ccAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATggg    >  1:1646153/1‑150 (MQ=255)
                                                                                                                                                    ccAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATggg    <  2:1172131/150‑1 (MQ=255)
                                                                                                                                                      aGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGtt  <  1:1506730/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGCGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGTT  >  NC_000913/464207‑464505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: