Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,655,944 A→G 6.2% intergenic (‑42/+22) yhiD ← / ← hdeB putative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,9440AG 6.2% 436.8 / 12.4 145intergenic (‑42/+22)yhiD/hdeBputative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (69/67);  new base G (5/4);  total (74/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 145 total aligned reads displayed.

ACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCG  >  NC_000913/3655796‑3656092
                                                                                                                                                    |                                                                                                                                                    
aCATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTTCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTAc                                                                                                                                                     <  1:1305387/150‑1 (MQ=255)
 cATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACt                                                                                                                                                    >  2:723285/1‑150 (MQ=255)
   tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                                                  <  2:140190/150‑1 (MQ=39)
   tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTcc                                                                                                                                                  <  2:1356850/150‑1 (MQ=39)
    ttgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGTAAATTGCCGCGAGTATAAGACGTATAATAAATTCTTCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCt                                                                                                                                                 <  1:1001279/149‑1 (MQ=255)
     tgtgCGTAACCCTGCTCCATTGCCGCGCATTTGCCTATCCATGCGAATAGCGCCACTGGCATTTGCCGCGAGTATAAGACGTCTAATAAATTCTGCTGTCATATAAAACTGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGATGCTCCtt                                                                                                                                                <  1:1068863/150‑1 (MQ=255)
        gCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCttttt                                                                                                                                             >  1:1733382/1‑150 (MQ=255)
        gCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCttttt                                                                                                                                             >  1:1265598/1‑150 (MQ=255)
              cccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGc                                                                                                                                       >  2:1638511/1‑150 (MQ=255)
              cccTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGc                                                                                                                                       <  2:1436492/150‑1 (MQ=255)
                cTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCACCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAc                                                                                                                                     >  1:1343993/1‑150 (MQ=255)
                 tGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAcc                                                                                                                                    >  1:761988/1‑150 (MQ=255)
                    tCCTTTGCCGCGCTTTTGCCTTTCCATGCCAATTGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATTATAAATTGTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCa                                                                                                                                 <  1:593340/150‑1 (MQ=255)
                       tttGCCGCGCATTTGCCTTTCCATGCCAATATCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGGCATATAAAAAAGCAAAAAGGATCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                                                                                                              <  1:157940/150‑1 (MQ=255)
                       tttGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                                                                                                              <  1:1056897/150‑1 (MQ=255)
                          gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                                           >  1:1414215/1‑150 (MQ=255)
                          gCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAtt                                                                                                                           >  2:479791/1‑150 (MQ=255)
                           ccGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTc                                                                                                                          <  2:51394/150‑1 (MQ=255)
                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                         <  1:70555/150‑1 (MQ=255)
                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                         >  1:1689480/1‑150 (MQ=255)
                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                         >  1:1349447/1‑150 (MQ=255)
                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCg                                                                                                                         >  1:64633/1‑150 (MQ=255)
                            cgcgCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTATTTACTTGCACCTCATTAAttag                                                                                                                         >  1:229402/1‑148 (MQ=255)
                              cgcATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGc                                                                                                                       <  1:1028545/150‑1 (MQ=255)
                                 ctttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACCTATAATAACATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCCAGATGGCTCAACTCGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                                                                                                    <  2:859830/149‑1 (MQ=255)
                                     gCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTAGCACCTCATTAATTCGGCAAGTCAt                                                                                                                >  2:1071377/1‑150 (MQ=255)
                                      ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCAtt                                                                                                               <  2:1008689/150‑1 (MQ=255)
                                       cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                                              >  2:201236/1‑150 (MQ=255)
                                       cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                                              >  2:1412964/1‑150 (MQ=255)
                                         ttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTatt                                                                                                            >  2:771383/1‑148 (MQ=255)
                                            cATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGc                                                                                                         >  2:310399/1‑150 (MQ=255)
                                            cATGCCAAAAGCGCCACACGCAATTGCCGAGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGAAGGATCAACTTGCTACTACTTTTTACTTGCACCTCATTAATACGGCAAGTCATTAGATGc                                                                                                         >  2:449367/1‑150 (MQ=255)
                                             aTGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCt                                                                                                        >  1:89203/1‑150 (MQ=255)
                                              cggcAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCtt                                                                                                       >  1:1083105/4‑150 (MQ=255)
                                               gCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTg                                                                                                      <  2:416551/150‑1 (MQ=255)
                                                    tAGCGCCACAGGCAATTGACGCGATTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCATCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAtttt                                                                                                 <  1:1307930/150‑1 (MQ=255)
                                                      gagcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGTCAAGTCATTAGATGCTTGATTTTTg                                                                                               <  2:1176439/148‑1 (MQ=255)
                                                       cgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGa                                                                                              >  2:736728/1‑150 (MQ=255)
                                                        gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                                             >  1:480075/1‑150 (MQ=255)
                                                        gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                                             >  1:1427173/1‑150 (MQ=255)
                                                        gcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAAATCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGaa                                                                                             <  1:442053/150‑1 (MQ=255)
                                                         ccAAAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAg                                                                                            <  2:1136890/150‑1 (MQ=255)
                                                          cacaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAgg                                                                                           >  2:1419014/1‑150 (MQ=255)
                                                             aGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGtat                                                                                        <  2:1567062/150‑1 (MQ=255)
                                                              ggCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTTTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTACTTTTTACTTGCACATCATTAATTCGGCAAGTCATTAGATGCTAGATTTTTGAAGGtata                                                                                       >  1:245383/1‑150 (MQ=255)
                                                               gaaaTTGCATCGAGTTTAAGACTTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGTTATAc                                                                                      <  1:314530/148‑1 (MQ=255)
                                                               gCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATAc                                                                                      >  1:278420/1‑150 (MQ=255)
                                                                        gcgAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTc                                                                             <  2:1459772/150‑1 (MQ=255)
                                                                           aGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGc                                                                          <  1:1488352/150‑1 (MQ=255)
                                                                              ataAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCggg                                                                       <  2:1305674/150‑1 (MQ=255)
                                                                                    cGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAAGTTGCTACTCCTTTTTACTTGCACCACATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttttta                                                                 >  2:712088/1‑149 (MQ=255)
                                                                                      tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                               <  2:914657/150‑1 (MQ=255)
                                                                                      tataATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCtt                                                               >  2:969211/1‑150 (MQ=255)
                                                                                       ataataAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTa                                                              >  1:1310001/1‑150 (MQ=255)
                                                                                        taataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTAc                                                             <  2:398013/150‑1 (MQ=255)
                                                                                          ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                                           <  2:1733382/150‑1 (MQ=255)
                                                                                          ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGACAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                                           >  1:784412/1‑150 (MQ=255)
                                                                                              aTTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTATTTACTTGCACCTCATTAATTCGGCAAGGCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTAtt                                                       <  2:1542898/150‑1 (MQ=255)
                                                                                               ttCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTc                                                      <  2:1291433/150‑1 (MQ=255)
                                                                                                  tgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGAt                                                   <  2:34042/150‑1 (MQ=255)
                                                                                                    ctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCa                                                 <  2:1690252/150‑1 (MQ=255)
                                                                                                      gTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACt                                               >  2:375615/1‑150 (MQ=255)
                                                                                                        cATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACttt                                             <  2:610958/150‑1 (MQ=255)
                                                                                                         atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTa                                            >  1:353389/1‑150 (MQ=255)
                                                                                                         atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTa                                            >  1:541352/1‑150 (MQ=255)
                                                                                                         atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTAAACTTGCTACTCCTTTTTCCTTGCACCTCATTAATTCGTCAAGTCATTAGAGGCTTGATTTTTGTAGGTATAAAAATTTTTCTGAGTGTTTTTCTTAAATTATTCTATCACtttt                                            >  2:273832/1‑149 (MQ=255)
                                                                                                          tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCGACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAg                                           <  1:78567/150‑1 (MQ=255)
                                                                                                             aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGaa                                        >  2:1325146/1‑150 (MQ=255)
                                                                                                             aaaaaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATCCTTGATTTTTGAAGGTAAACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGTaa                                        >  2:640523/1‑150 (MQ=255)
                                                                                                                  tGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtga                                   >  2:811841/1‑150 (MQ=255)
                                                                                                                   gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgat                                  >  1:342294/1‑149 (MQ=255)
                                                                                                                   gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgag                                  >  1:1704247/1‑150 (MQ=255)
                                                                                                                   gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgag                                  >  1:422897/1‑150 (MQ=255)
                                                                                                                     aaaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg                                >  1:1560283/1‑150 (MQ=255)
                                                                                                                      aaaaGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga                               >  1:289291/1‑150 (MQ=38)
                                                                                                                      aaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga                               <  2:791768/150‑1 (MQ=255)
                                                                                                                      aaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCATTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtga                               >  1:1108035/1‑150 (MQ=255)
                                                                                                                        aagaagTAGCAAGATGAGACATATTGCTGCTCCTTTTTACTTGCACCTAATTAATTCGGAAAGTCATTTGATTCTTGATTTTTGAAGGTATACAAATTTTTCTGAGTGTTTTTCTTACAGTATTAGATCACTTTAGTAATTTGATTgaga                             >  1:292548/1‑150 (MQ=255)
                                                                                                                        aaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTgaga                             <  2:164335/150‑1 (MQ=255)
                                                                                                                         aGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGAt                            >  2:441612/1‑150 (MQ=39)
                                                                                                                          ggAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACATCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATc                           >  2:1325274/1‑150 (MQ=38)
                                                                                                                               agcaAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGttt                      <  1:482323/150‑1 (MQ=255)
                                                                                                                                gcaAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTc                     >  1:897573/1‑150 (MQ=255)
                                                                                                                                 caAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCa                    >  2:1692017/1‑150 (MQ=255)
                                                                                                                                   aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt                  <  2:952892/150‑1 (MQ=255)
                                                                                                                                   aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt                  <  2:539240/150‑1 (MQ=255)
                                                                                                                                   aGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAtt                  <  1:1275209/150‑1 (MQ=255)
                                                                                                                                    gATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAttt                 <  1:996961/150‑1 (MQ=255)
                                                                                                                                     aTGGCTCAACTTTCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTa                <  1:1665943/150‑1 (MQ=255)
                                                                                                                                      tGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTaa               <  2:1689480/150‑1 (MQ=255)
                                                                                                                                        gCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAg             <  2:897573/150‑1 (MQ=255)
                                                                                                                                        gCTCAACTTGCTACTCCATTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTaaat             >  1:999520/1‑149 (MQ=255)
                                                                                                                                           cAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAAATATTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTaa          >  2:1041289/1‑150 (MQ=255)
                                                                                                                                             aCTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACg        >  1:1114244/1‑150 (MQ=255)
                                                                                                                                                tGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTa     <  2:288492/150‑1 (MQ=255)
                                                                                                                                                tGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTa     >  2:1134713/1‑150 (MQ=255)
                                                                                                                                                  cTACTCCTTTTTACTTGCAAATCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAATGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATc   <  2:1222635/150‑1 (MQ=255)
                                                                                                                                                   tACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGATGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCg  <  1:1363686/150‑1 (MQ=255)
                                                                                                                                                   tACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCg  <  2:534004/150‑1 (MQ=255)
                                                                                                                                                   tACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCg  <  2:1355353/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCG  >  NC_000913/3655796‑3656092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: