Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,663,564 G→A 10.1% intergenic (+37/+326) mdtF → / ← gadW anaerobic multidrug efflux transporter, ArcA‑regulated/transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,663,5640GA10.1% 341.6 / 20.0 118intergenic (+37/+326)mdtF/gadWanaerobic multidrug efflux transporter, ArcA‑regulated/transcriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base G (59/47);  new base A (3/9);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 6.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 120 total aligned reads displayed.

TAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCGTAATC  >  NC_000913/3663415‑3663713
                                                                                                                                                     |                                                                                                                                                     
taggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAg                                                                                                                                                       <  2:1316485/150‑1 (MQ=255)
  ggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCTGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGcc                                                                                                                                                     <  1:1458373/150‑1 (MQ=255)
    taCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGccct                                                                                                                                                   <  2:829334/150‑3 (MQ=255)
    taCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCtt                                                                                                                                                   <  2:1470498/150‑1 (MQ=255)
     aCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTa                                                                                                                                                  >  2:118266/1‑150 (MQ=255)
      ccGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTAc                                                                                                                                                 <  2:835611/150‑1 (MQ=255)
      ccGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTAc                                                                                                                                                 <  2:674534/150‑1 (MQ=255)
      ccGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTAc                                                                                                                                                 <  1:1613909/150‑1 (MQ=255)
      ccGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTAc                                                                                                                                                 <  2:148098/150‑1 (MQ=255)
      ccGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTAc                                                                                                                                                 <  1:1663048/150‑1 (MQ=255)
        ggCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTActc                                                                                                                                               <  1:892449/150‑1 (MQ=255)
        ggCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTActc                                                                                                                                               <  1:322503/150‑1 (MQ=255)
         gCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTActct                                                                                                                                              <  2:1593471/150‑1 (MQ=39)
         gCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTActct                                                                                                                                              <  1:764627/150‑1 (MQ=39)
         gCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTActct                                                                                                                                              <  1:1302946/150‑1 (MQ=39)
         gCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGCTGAACCTTCATGTTCAACCTTActct                                                                                                                                              <  1:860814/150‑1 (MQ=39)
          ggggctGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCtt                                                                                                                                             <  1:350500/145‑1 (MQ=255)
           gTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCttt                                                                                                                                            >  2:1139838/1‑150 (MQ=255)
            tGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTc                                                                                                                                           <  1:570824/150‑1 (MQ=255)
               tGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTg                                                                                                                                        >  2:1364488/1‑150 (MQ=255)
                    gggATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAAc                                                                                                                                   <  1:374636/150‑1 (MQ=255)
                         gTTTGCCGCAACAGTGCTGGCAATTTACTTCGATCCTGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTc                                                                                                                              <  1:1590920/150‑1 (MQ=255)
                            tGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcacc                                                                                                                           >  2:50463/1‑150 (MQ=255)
                            tGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGGCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcacc                                                                                                                           <  2:350608/150‑1 (MQ=255)
                             gCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcacca                                                                                                                          <  1:700398/150‑1 (MQ=255)
                                gCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCCCCACTc                                                                                                                       >  1:1487911/1‑150 (MQ=255)
                                gCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTc                                                                                                                       >  1:1494714/1‑150 (MQ=255)
                                    cAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcaaca                                                                                                                   <  2:1518880/150‑1 (MQ=255)
                                         cTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAg                                                                                                              >  2:215341/1‑150 (MQ=255)
                                          tGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGc                                                                                                             <  2:1644411/150‑1 (MQ=255)
                                          tGGCAATATACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGc                                                                                                             >  2:509529/1‑150 (MQ=255)
                                           ggCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCt                                                                                                            >  2:1605138/1‑150 (MQ=255)
                                           ggCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCt                                                                                                            <  2:19639/150‑1 (MQ=255)
                                             cAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTaa                                                                                                          >  1:871051/1‑150 (MQ=255)
                                                  tACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTc                                                                                                     >  2:1373285/1‑150 (MQ=255)
                                                  tACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACAATTCAACACTCAACAACCAGCTAACCggc                                                                                                     >  1:300390/1‑148 (MQ=255)
                                                     ttCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAgg                                                                                                  <  1:638500/150‑1 (MQ=255)
                                                           ccGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATAcc                                                                                            <  1:1139838/150‑1 (MQ=255)
                                                            cGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGTTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCa                                                                                           >  2:1239501/1‑150 (MQ=255)
                                                             ggTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAg                                                                                          >  1:185157/1‑150 (MQ=255)
                                                               tCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGc                                                                                        <  1:494732/150‑1 (MQ=255)
                                                                  ttttCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTa                                                                                     <  2:691757/150‑1 (MQ=255)
                                                                   tttCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAg                                                                                    <  1:155518/150‑1 (MQ=255)
                                                                       gTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTaca                                                                                >  1:1484229/1‑150 (MQ=255)
                                                                       gTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTATAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTaca                                                                                >  1:833481/1‑150 (MQ=255)
                                                                        ttGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTacac                                                                               <  2:417450/150‑1 (MQ=255)
                                                                          gTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAg                                                                             <  2:1493685/150‑1 (MQ=255)
                                                                           tAGTGGTACATCTCTTTTCCCTCTTTAAATAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCTCCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGc                                                                            <  1:1201688/150‑1 (MQ=255)
                                                                           tAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGc                                                                            <  1:453734/150‑1 (MQ=255)
                                                                               ggAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCTCTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTGCACAGCACTg                                                                        >  2:210623/1‑150 (MQ=255)
                                                                               ggAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTg                                                                        >  1:509792/1‑150 (MQ=255)
                                                                                taaCATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGa                                                                       <  1:707022/149‑1 (MQ=255)
                                                                                gAACATCTCTTTGCACGCTTTAAAAAAGCGTAAAGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTCCTAACCATTCACCACTCAACAAACAGCTAACCGTCAGGAATACCAGGCATAGTACACAGCACtta                                                                       >  1:542492/1‑148 (MQ=255)
                                                                                 aaCATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGaa                                                                      <  1:1667992/150‑1 (MQ=255)
                                                                                   cATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAg                                                                    <  2:909512/150‑1 (MQ=255)
                                                                                      ctctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAg                                                                 >  1:1499104/1‑150 (MQ=255)
                                                                                      ctctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAg                                                                 <  2:691127/150‑1 (MQ=255)
                                                                                       tctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGTGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACGACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGa                                                                <  2:397556/150‑1 (MQ=255)
                                                                                          ttGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAc                                                             <  1:249492/150‑1 (MQ=255)
                                                                                           tGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAcc                                                            <  2:1514815/150‑1 (MQ=255)
                                                                                                  tttAAATAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGGTCAGCCTTACTCTTTCCTGCTAACCATTCACGACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAg                                                     <  1:645726/150‑1 (MQ=255)
                                                                                                   ttAAAAAATCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGc                                                    <  2:1247661/150‑1 (MQ=255)
                                                                                                   ttAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCCGCTAACCGTCAGGAATACCAGGCTTAGTACCCAGCACTGAAAGTAGAAACCACTCCAGc                                                    >  2:849351/1‑150 (MQ=255)
                                                                                                     aaaaaaGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCa                                                  >  2:1676569/1‑150 (MQ=255)
                                                                                                       aaaaGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGCAACCACTCCAGCCAtt                                                >  2:883611/1‑150 (MQ=255)
                                                                                                       aaaaGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAACCCACTCCAGCCAtt                                                >  2:872103/1‑150 (MQ=255)
                                                                                                            cGTATCGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtca                                           <  1:692558/150‑1 (MQ=255)
                                                                                                             gTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtcat                                          >  2:338790/1‑150 (MQ=255)
                                                                                                              tAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtcatc                                         >  2:1585500/1‑150 (MQ=255)
                                                                                                               aaCGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtcatca                                        >  1:1615899/1‑150 (MQ=255)
                                                                                                                aCGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCaa                                       >  1:708331/1‑150 (MQ=255)
                                                                                                                  gtgtAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACACCCAGCTAACCGTCAGGAATACCAGGCTTAGTACAAAGCACTGAAAGTAGAAACAACTCCAGCCATTCGTCATCAAtt                                     >  2:1337670/1‑150 (MQ=255)
                                                                                                                  gtgtAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAAtt                                     >  2:590820/1‑150 (MQ=255)
                                                                                                                    gtAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTg                                   >  1:1469285/1‑150 (MQ=255)
                                                                                                                      aaaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTATCCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaa                                 >  2:400805/1‑150 (MQ=255)
                                                                                                                      aaaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaa                                 >  1:728368/1‑150 (MQ=255)
                                                                                                                       ggaaagagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTTCTAACCATTCACCACTCAACCACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCCCTCCAGCCATTCGTCATCAATTTGaac                                >  2:1602447/5‑150 (MQ=37)
                                                                                                                       aaTGAGAGTAAGGATGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaac                                <  1:1436778/150‑1 (MQ=255)
                                                                                                                        aTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaaca                               <  1:50463/150‑1 (MQ=255)
                                                                                                                         tGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCCACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAACCCACTCCAGCCATTCGTCATCAATTTGaacac                              >  2:935977/1‑149 (MQ=255)
                                                                                                                          gagaGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTTGTCATCAATTTGaacaac                             >  2:986866/1‑150 (MQ=255)
                                                                                                                           agagTAAGGTTGAACTTGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGTATACCAGGCTTAGTACACAGCACTGAAAGTAGAAGCCACTACAGCCATTCGTCATCAATTTGaacaacc                            >  1:1076694/1‑149 (MQ=255)
                                                                                                                           agagTAAGGCTGAACCTTAATCTGCAACCTTACTCTTTCCAGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGATTACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCCCTCAAGCCCTTCGTCATCAATTTGAaaaaca                            >  2:1175727/1‑150 (MQ=255)
                                                                                                                            gagTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAacac                           >  1:614701/1‑150 (MQ=255)
                                                                                                                            gagTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAacac                           <  1:437784/150‑1 (MQ=255)
                                                                                                                                aaGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTACCCATTCACCACTCACCAACCAGCTACCCGTCAGGAATACCAGGCTTAGTACACAGCCCTGAAAGTAGACACCCCTCCAGCCATTCGTCATCAATTTGcacaacacaaga                       >  1:1717682/1‑148 (MQ=255)
                                                                                                                                  ggTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTTGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACt                     >  1:1510972/1‑150 (MQ=255)
                                                                                                                                   gTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCCGCTAACCGTCAGGAATACCAGGCTAAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACtc                    >  2:1348457/1‑149 (MQ=255)
                                                                                                                                      gAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAAttt                                    <  2:1699312/131‑1 (MQ=255)
                                                                                                                                      gAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAAttt                                    >  1:1699312/1‑131 (MQ=255)
                                                                                                                                      gAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGAcc                 <  2:532774/150‑1 (MQ=255)
                                                                                                                                      gAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGAcc                 >  2:543610/1‑150 (MQ=255)
                                                                                                                                             aaGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCCGCCATTCGTCATCAATTTGAAAAACACAATACTGACCCACATTc          >  1:1520479/1‑150 (MQ=255)
                                                                                                                                              aGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTcc         >  2:954398/1‑150 (MQ=255)
                                                                                                                                              aGGTTCAGCCTTACTCTTTCCTCCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTACAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTcc         >  2:948262/1‑150 (MQ=255)
                                                                                                                                               ggTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCCGCCATTCGTCATCAAATTGAACAACACAATACTGACCCACATTcac        >  1:1653493/1‑148 (MQ=255)
                                                                                                                                                gTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCg       >  1:1331037/1‑150 (MQ=255)
                                                                                                                                                  tCAGCCTTACTCTTTCATGCTAACCATTCACCACTCAACAACCAGCTAAACGTCAGGTATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCAAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCGTa     <  1:1623122/150‑1 (MQ=255)
                                                                                                                                                    aGCCTTACTCTTTCCTGCTAACCATTAACCACTCAACAACCAGCTAACCGTCAGGAATAACAGGCTTAGTACACAGCACTGATAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCGTAAt   <  2:542492/150‑1 (MQ=255)
                                                                                                                                                     gCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGCCCCACATTCCCGTAATc  >  2:682014/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTCCCGTAATC  >  NC_000913/3663415‑3663713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: