Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,674,389 C→A 5.7% intergenic (+14/‑397) yhjD → / → yhjE inner membrane putative BrbK family alternate lipid exporter/putative MFS transporter; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,674,3890CA 5.7% 316.6 / 5.9 114intergenic (+14/‑397)yhjD/yhjEinner membrane putative BrbK family alternate lipid exporter/putative MFS transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base C (50/57);  new base A (4/3);  total (54/60)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 115 total aligned reads displayed.

CAGCATTTGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGA  >  NC_000913/3674242‑3674535
                                                                                                                                                   |                                                                                                                                                  
cagcaTTTGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATgcgg                                                                                                                                                  >  2:1518840/1‑150 (MQ=255)
   caTTTGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGt                                                                                                                                               >  2:75384/1‑150 (MQ=255)
        ggTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGAGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACg                                                                                                                                          <  2:560031/150‑1 (MQ=255)
          ttCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGcc                                                                                                                                        <  1:652355/150‑1 (MQ=255)
          tgccGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGcc                                                                                                                                        <  2:1097103/148‑1 (MQ=255)
          gtCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGATTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGcc                                                                                                                                        <  1:718148/149‑1 (MQ=255)
            ccGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTGTGACGCTGTTTTGCGCGGCGGGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCtt                                                                                                                                      <  2:664594/150‑1 (MQ=255)
               ggCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                   <  2:1118260/149‑1 (MQ=255)
                gCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                  >  2:51016/1‑150 (MQ=255)
                    gggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGcc                                                                                                                              <  1:1676193/150‑2 (MQ=255)
                    gggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGcc                                                                                                                              <  1:1245465/150‑2 (MQ=255)
                    gggCGGTTGGCAGTATCCTACTGCTTCGCCCGTGGGACGCTGTTTTGCGCGGCGGGGATTGCCACCTCCGAATATAAAGACGACCCGGGAATGCCGGGGAAATCGGAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGcc                                                                                                                              <  2:243146/150‑2 (MQ=255)
                     ggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCAt                                                                                                                             >  1:631452/1‑150 (MQ=255)
                     ggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCAt                                                                                                                             <  1:1233788/150‑1 (MQ=255)
                       cTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATAc                                                                                                                           >  2:1466903/1‑150 (MQ=255)
                         gATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGa                                                                                                                         >  1:280224/1‑150 (MQ=255)
                         gATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGa                                                                                                                         >  2:1330706/1‑150 (MQ=255)
                            ggCATTTTTCTACTTCTTCGCCCGCTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAttt                                                                                                                      <  1:475200/150‑1 (MQ=255)
                             gCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtttt                                                                                                                     >  1:933315/1‑150 (MQ=255)
                             gCATTTTTCTACTTCTTCGCCCGTTTGACGCTGCTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtttt                                                                                                                     <  1:1142913/150‑1 (MQ=255)
                               ctctttCTACTTCTTCGCCCGTCTGACGCCGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCTGCGTAAACGCCTTATCCGGCATACGATTTTAt                                                                                                                   <  1:1144389/147‑1 (MQ=255)
                                  tttCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCg                                                                                                                <  1:322661/150‑1 (MQ=255)
                                     cTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGcc                                                                                                             <  1:1377622/150‑1 (MQ=255)
                                     cTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGcc                                                                                                             >  2:638259/1‑150 (MQ=255)
                                       aCTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTg                                                                                                           >  1:594462/1‑150 (MQ=255)
                                       aCTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTg                                                                                                           >  1:42114/1‑150 (MQ=255)
                                        cttcttCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTgg                                                                                                          >  2:1150736/1‑150 (MQ=255)
                                        cttcttCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTgg                                                                                                          >  2:1601081/1‑150 (MQ=255)
                                         ttcttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGc                                                                                                         >  1:1424683/1‑150 (MQ=255)
                                          tcttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAACCGCAGCCTTAAACTCGGCCGGATGCGGCGTAAACGCCGTATCCGGCATACGATTTTATTCGGCCTGGCt                                                                                                        <  1:291776/150‑1 (MQ=255)
                                          tcttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCt                                                                                                        >  1:255744/1‑150 (MQ=255)
                                           cttcGCCCGTAAGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGAGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTc                                                                                                       >  2:937337/1‑150 (MQ=255)
                                            ttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAACGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTcc                                                                                                      <  1:1254000/150‑1 (MQ=255)
                                             tcGCCCGTTTGACGCTGGTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTccc                                                                                                     <  2:306540/150‑1 (MQ=255)
                                             tcGCCCGTTTGACGCGGTGTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTccc                                                                                                     <  1:174774/150‑1 (MQ=255)
                                              cGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTcccc                                                                                                    <  2:1421708/150‑1 (MQ=255)
                                                cccGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGt                                                                                                  >  2:773236/1‑150 (MQ=255)
                                                  cGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAg                                                                                                >  1:268539/1‑150 (MQ=255)
                                                  cGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAg                                                                                                <  1:243272/150‑1 (MQ=255)
                                                  cGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAg                                                                                                >  1:945364/1‑150 (MQ=255)
                                                    tttGCCGCGGTTTTGCGCGGCGTGTATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGTGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGc                                                                                              <  2:1061015/150‑1 (MQ=255)
                                                         cgccggcttGCGCGGCGTGGAAGGCCCGCGCCGAATATAAACCCGACCAGCGACTCCCGCGGAAACCCCAGCCTTCAAATAGGCCGGATGCGGCGTAACCGCCTTATCCGGAATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGAt                                                                                         <  1:152757/143‑1 (MQ=255)
                                                         cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGAt                                                                                         >  2:1691266/1‑150 (MQ=255)
                                                          gCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATa                                                                                        >  1:247512/1‑150 (MQ=255)
                                                            tGTTTTGCGAGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAg                                                                                      <  2:840350/150‑1 (MQ=255)
                                                             gTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAACGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGa                                                                                     <  1:1465619/150‑1 (MQ=255)
                                                             gTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGa                                                                                     <  1:763463/150‑1 (MQ=255)
                                                             gTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGa                                                                                     <  2:748875/150‑1 (MQ=255)
                                                                ttGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATgc                                                                                  <  1:1196725/150‑1 (MQ=255)
                                                                   cgcgGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGcc                                                                               <  2:1233859/150‑1 (MQ=255)
                                                                        cgTGGATTGCCACCGCCGAATATAAAGACTACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCAGGATAAGATGCGCCAgcat                                                                          >  1:1257677/1‑150 (MQ=255)
                                                                          gggATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATCGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcg                                                                        <  2:1630633/149‑1 (MQ=255)
                                                                          gggATTGCCACCGCCGAATATAAAGACGACCCGCGAAAGCCGGGGAAAACGCAGCCTAAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcg                                                                        <  1:957088/149‑1 (MQ=255)
                                                                           ggATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcgc                                                                       <  2:1651775/150‑1 (MQ=255)
                                                                             aTTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCAGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcgcat                                                                     >  1:145000/1‑150 (MQ=255)
                                                                               tGCCACCGCCGAATATACAGACGCCCAGCGCATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATcc                                                                   <  2:1671828/150‑1 (MQ=255)
                                                                                gCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACACCTTATCCGGCATACGATTTTATTCGGCCTGGCACCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCg                                                                  >  1:710014/1‑150 (MQ=255)
                                                                                 ccACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCgg                                                                 >  2:1680497/1‑150 (MQ=255)
                                                                                 ccACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCgg                                                                 >  2:628479/1‑150 (MQ=255)
                                                                                     cgccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCtat                                                             <  2:1398587/150‑1 (MQ=255)
                                                                                     cgccgAATAAACAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCtat                                                             <  2:274765/150‑1 (MQ=255)
                                                                                      gccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAATTAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCTGCATCGCATCCTGCtata                                                            <  2:1109704/150‑1 (MQ=255)
                                                                                        cgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGCTAAGATGCGCCAGCATCGCATCCGGCTATAAt                                                          >  1:1314263/1‑150 (MQ=255)
                                                                                             ataAAGACGCCCCGCGAATGCCGGGGAAAACCCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGca                                                     <  1:111029/150‑1 (MQ=255)
                                                                                             ataAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGca                                                     <  1:666785/150‑1 (MQ=255)
                                                                                              taAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGcac                                                    >  2:670368/1‑150 (MQ=255)
                                                                                               aaaGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATTCGcaca                                                   >  2:305094/1‑150 (MQ=255)
                                                                                               aaaGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGcaca                                                   <  1:1150736/150‑1 (MQ=255)
                                                                                                  gacgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATaa                                                >  1:363636/1‑150 (MQ=255)
                                                                                                    cgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                              >  1:801958/1‑150 (MQ=255)
                                                                                                    cgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                              <  1:1414949/150‑1 (MQ=255)
                                                                                                        ccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGCTTTTATTCGGCCTGGCTCCCCGAAGGCCGGATAAGCTGCGCCAGCATCGCATCCGTCTATAATTCGCACATAACCTCtt                                          >  1:886796/1‑150 (MQ=255)
                                                                                                           cgAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGaa                                       <  2:66789/150‑1 (MQ=255)
                                                                                                              aTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACt                                    <  2:1538759/150‑1 (MQ=255)
                                                                                                              aTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACt                                    <  1:137346/150‑1 (MQ=255)
                                                                                                              aTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACt                                    <  2:452753/150‑1 (MQ=255)
                                                                                                                gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCg                                  <  1:1518840/150‑1 (MQ=255)
                                                                                                                gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCg                                  <  2:1693991/150‑1 (MQ=255)
                                                                                                                gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCg                                  <  1:1610627/150‑1 (MQ=255)
                                                                                                                 acGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCCCATAACCTCTTGAAACTCGt                                 >  1:1316511/2‑150 (MQ=255)
                                                                                                                   ggggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTcc                               >  1:1049720/1‑150 (MQ=255)
                                                                                                                   ggggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTcc                               >  2:1170620/1‑150 (MQ=255)
                                                                                                                    gggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTccc                              <  2:1621452/150‑1 (MQ=255)
                                                                                                                         aaCGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCagag                         <  1:1595377/150‑1 (MQ=255)
                                                                                                                          aCGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGc                        >  2:25982/1‑150 (MQ=255)
                                                                                                                           cGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGcc                       <  1:1002963/150‑1 (MQ=255)
                                                                                                                           cGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGcc                       <  1:1619968/150‑1 (MQ=255)
                                                                                                                                ccTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTc                  >  2:1590504/1‑150 (MQ=255)
                                                                                                                                 cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                 >  1:641956/1‑150 (MQ=255)
                                                                                                                                 cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                 >  1:1149526/1‑150 (MQ=255)
                                                                                                                                 cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                 >  1:457814/1‑150 (MQ=255)
                                                                                                                                 cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCGTcc                 >  1:861369/1‑149 (MQ=255)
                                                                                                                                     aaaTAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCa             <  1:51016/150‑1 (MQ=255)
                                                                                                                                         aGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATGTa         >  2:1109854/1‑150 (MQ=255)
                                                                                                                                          tgCCGGATAAGGCGTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCatatat        >  2:1160732/2‑150 (MQ=38)
                                                                                                                                          tgCCGGATAAGGCGTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTAt        >  2:1153283/2‑150 (MQ=39)
                                                                                                                                          tgCCGGATAAGGCGTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTAt        >  2:1545918/2‑150 (MQ=39)
                                                                                                                                               gATGCGGCGTAAACGCCTTCTCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTggg   <  2:880049/150‑1 (MQ=255)
                                                                                                                                                aTGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGa  <  1:506141/150‑1 (MQ=255)
                                                                                                                                                aTGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGa  <  2:673359/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCATTTGGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGA  >  NC_000913/3674242‑3674535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: