Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,674,396 A→T 6.7% intergenic (+21/‑390) yhjD → / → yhjE inner membrane putative BrbK family alternate lipid exporter/putative MFS transporter; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,674,3960AT 6.7% 345.6 / 10.6 119intergenic (+21/‑390)yhjD/yhjEinner membrane putative BrbK family alternate lipid exporter/putative MFS transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base A (52/59);  new base T (5/3);  total (57/62)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 125 total aligned reads displayed.

GGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATT  >  NC_000913/3674250‑3674545
                                                                                                                                                  |                                                                                                                                                     
ggTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGAGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACg                                                                                                                                                    <  2:560031/150‑1 (MQ=255)
  ttCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGcc                                                                                                                                                  <  1:652355/150‑1 (MQ=255)
  tgccGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGcc                                                                                                                                                  <  2:1097103/148‑1 (MQ=255)
       ggCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                             <  2:1118260/149‑1 (MQ=255)
        gCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                            >  2:51016/1‑150 (MQ=255)
          tGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCGGTTTTGCGCGGCGTGTATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                          <  2:1457277/150‑1 (MQ=255)
            gggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGcc                                                                                                                                        <  1:1676193/150‑2 (MQ=255)
            gggCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATAAGGCGTTTACGCCGCATCCGGcc                                                                                                                                        <  1:1245465/150‑2 (MQ=255)
              gCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTTGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATa                                                                                                                                      <  1:1166247/150‑1 (MQ=255)
                 gATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGa                                                                                                                                   >  1:280224/1‑150 (MQ=255)
                    ggCATTTTTCTACTTCTTCGCCCGCTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAttt                                                                                                                                <  1:475200/150‑1 (MQ=255)
                     gCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtttt                                                                                                                               >  1:933315/1‑150 (MQ=255)
                     gCATTTTTCTACTTCTTCGCCCGTTTGACGCTGCTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGAtttt                                                                                                                               <  1:1142913/150‑1 (MQ=255)
                         ttttCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTc                                                                                                                           <  1:1330706/150‑1 (MQ=255)
                          tttCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCg                                                                                                                          <  1:322661/150‑1 (MQ=255)
                             cTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGcc                                                                                                                       <  1:1377622/150‑1 (MQ=255)
                             cTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGcc                                                                                                                       >  2:638259/1‑150 (MQ=255)
                               aCTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTg                                                                                                                     >  1:594462/1‑150 (MQ=255)
                               aCTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTg                                                                                                                     >  1:42114/1‑150 (MQ=255)
                                cttcttCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTgg                                                                                                                    >  2:1150736/1‑150 (MQ=255)
                                cttcttCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTgg                                                                                                                    >  2:1601081/1‑150 (MQ=255)
                                  tcttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAACCGCAGCCTTAAACTCGGCCGGATGCGGCGTAAACGCCGTATCCGGCATACGATTTTATTCGGCCTGGCt                                                                                                                  <  1:291776/150‑1 (MQ=255)
                                   cttcGCCCGTAAGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGAGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTc                                                                                                                 >  2:937337/1‑150 (MQ=255)
                                    ttcGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAACGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTcc                                                                                                                <  1:1254000/150‑1 (MQ=255)
                                      cGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTcccc                                                                                                              <  2:1421708/150‑1 (MQ=255)
                                       gCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCg                                                                                                             >  2:1701540/1‑150 (MQ=255)
                                        cccGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGt                                                                                                            >  2:773236/1‑150 (MQ=255)
                                          cGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAg                                                                                                          >  1:945364/1‑150 (MQ=255)
                                          cGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAg                                                                                                          <  1:243272/150‑1 (MQ=255)
                                          cGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAg                                                                                                          >  1:268539/1‑150 (MQ=255)
                                            tttGCCGCGGTTTTGCGCGGCGTGTATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGTGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGc                                                                                                        <  2:1061015/150‑1 (MQ=255)
                                                 cGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGAt                                                                                                   >  2:1691266/1‑150 (MQ=255)
                                                  gCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATa                                                                                                  >  1:247512/1‑150 (MQ=255)
                                                    tGTTTTGCGAGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAg                                                                                                <  2:840350/150‑1 (MQ=255)
                                                     gTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAACGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGa                                                                                               <  1:1465619/150‑1 (MQ=255)
                                                     gTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGTAAAACGCACCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGa                                                                                               >  2:1624888/1‑150 (MQ=255)
                                                     gTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGa                                                                                               <  1:763463/150‑1 (MQ=255)
                                                        ttGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATgc                                                                                            <  1:1196725/150‑1 (MQ=255)
                                                           cgcgGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGcc                                                                                         <  2:1233859/150‑1 (MQ=255)
                                                                cgTGGATTGCCACCGCCGAATATAAAGACTACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCAGGATAAGATGCGCCAgcat                                                                                    >  1:1257677/1‑150 (MQ=255)
                                                                  gggATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATCGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcg                                                                                  <  2:1630633/149‑1 (MQ=255)
                                                                   ggATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcgc                                                                                 <  2:1651775/150‑1 (MQ=255)
                                                                     aTTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCAGGCTCCCCGTAGGCCGGATAAGATGCGCCAgcatcgcat                                                                               >  1:145000/1‑150 (MQ=255)
                                                                        gCCCCCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATACg                                                                            >  1:512482/1‑150 (MQ=255)
                                                                        gCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACACCTTATCCGGCATACGATTTTATTCGGCCTGGCACCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCg                                                                            >  1:710014/1‑150 (MQ=255)
                                                                         ccACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCgg                                                                           >  2:1680497/1‑150 (MQ=255)
                                                                         ccACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCgg                                                                           >  2:628479/1‑150 (MQ=255)
                                                                          cACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGc                                                                          >  1:207250/1‑150 (MQ=255)
                                                                           aCCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATGCGATGCTGGCGCATCTTATCCGGcc                                                                         <  1:75384/150‑2 (MQ=38)
                                                                             cgccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCtat                                                                       <  2:1398587/150‑1 (MQ=255)
                                                                             cgccgAATAAACAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCtat                                                                       <  2:274765/150‑1 (MQ=255)
                                                                              gccgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAATTAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCTGCATCGCATCCTGCtata                                                                      <  2:1109704/150‑1 (MQ=255)
                                                                                cgAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGCTAAGATGCGCCAGCATCGCATCCGGCTATAAt                                                                    >  1:1314263/1‑150 (MQ=255)
                                                                                     ataAAGACGCCCCGCGAATGCCGGGGAAAACCCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGca                                                               <  1:111029/150‑1 (MQ=255)
                                                                                     ataAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGca                                                               <  1:666785/150‑1 (MQ=255)
                                                                                      taAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGcac                                                              >  2:670368/1‑150 (MQ=255)
                                                                                       aaaGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATTCGcaca                                                             >  2:305094/1‑150 (MQ=255)
                                                                                       aaaGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGcaca                                                             <  1:1150736/150‑1 (MQ=255)
                                                                                       aaaGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGcaca                                                             >  2:101659/1‑150 (MQ=255)
                                                                                          gacgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATaa                                                          >  1:363636/1‑150 (MQ=255)
                                                                                            cgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                                        >  1:801958/1‑150 (MQ=255)
                                                                                            cgacCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAAcc                                                        <  1:1414949/150‑1 (MQ=255)
                                                                                               cccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACctct                                                     >  2:717798/1‑150 (MQ=255)
                                                                                                ccGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGCTTTTATTCGGCCTGGCTCCCCGAAGGCCGGATAAGCTGCGCCAGCATCGCATCCGTCTATAATTCGCACATAACCTCtt                                                    >  1:886796/1‑150 (MQ=255)
                                                                                                   cgAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGaa                                                 <  2:66789/150‑1 (MQ=255)
                                                                                                      aTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACt                                              <  2:452753/150‑1 (MQ=255)
                                                                                                      aTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACt                                              <  2:1538759/150‑1 (MQ=255)
                                                                                                      aTGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACt                                              <  1:137346/150‑1 (MQ=255)
                                                                                                        gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCg                                            <  2:1693991/150‑1 (MQ=255)
                                                                                                        gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCg                                            <  1:1518840/150‑1 (MQ=255)
                                                                                                        gCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCg                                            <  1:1610627/150‑1 (MQ=255)
                                                                                                          cGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTc                                          >  1:1508793/1‑150 (MQ=255)
                                                                                                           ggggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTcc                                         >  2:1170620/1‑150 (MQ=255)
                                                                                                           ggggAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTcc                                         >  1:1049720/1‑150 (MQ=255)
                                                                                                                 aaCGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCagag                                   <  1:1595377/150‑1 (MQ=255)
                                                                                                                  aCGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGc                                  >  2:25982/1‑150 (MQ=255)
                                                                                                                   cGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGcc                                 <  1:1002963/150‑1 (MQ=255)
                                                                                                                   cGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGcc                                 <  1:1619968/150‑1 (MQ=255)
                                                                                                                        ccTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTc                            >  2:1590504/1‑150 (MQ=255)
                                                                                                                        ccTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTc                            >  2:1152068/1‑150 (MQ=255)
                                                                                                                         cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                           >  1:457814/1‑150 (MQ=255)
                                                                                                                         cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                           >  1:1149526/1‑150 (MQ=255)
                                                                                                                         cTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCa                           >  1:641956/1‑150 (MQ=255)
                                                                                                                             aaaTAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCa                       <  1:51016/150‑1 (MQ=255)
                                                                                                                                  tgCCGGATAAGGCGTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCatatat                  >  2:1160732/2‑150 (MQ=38)
                                                                                                                                  tgCCGGATAAGGCGTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTAt                  >  2:1545918/2‑150 (MQ=39)
                                                                                                                                      ggataaggcgTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTgg              >  2:1639708/7‑150 (MQ=39)
                                                                                                                                      ggataaggcgTTTACGCCGCATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATAGCATCCGGCTATAAAGCGCACATAACCTCTTGAACCTCGTCCCCAGAGCCTCGTCAGCCATCTATTTgg              >  1:555289/7‑150 (MQ=38)
                                                                                                                                       gATGCGGCGTAAACGCCTTCTCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTggg             <  2:880049/150‑1 (MQ=255)
                                                                                                                                       gATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGCGCCTCTTCAGCCATCTATTTggg             >  2:523893/1‑150 (MQ=255)
                                                                                                                                        aTGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGa            <  2:673359/150‑1 (MQ=255)
                                                                                                                                            ggcgTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCaa        >  1:886700/1‑150 (MQ=255)
                                                                                                                                             gcgTAAAGGCCTTATGCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCaaa       <  2:1183174/150‑1 (MQ=255)
                                                                                                                                              cgTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAAc      <  2:280224/150‑1 (MQ=255)
                                                                                                                                               gTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACa     >  1:24943/1‑150 (MQ=255)
                                                                                                                                                 aaaCGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCTGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAAt   >  2:525808/1‑150 (MQ=255)
                                                                                                                                                 aaaCGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGCAACTCGTCCCCAGCGCCTCTTCAGCCATCTAGTTGGGAGCAAACAAt   >  2:1120424/1‑150 (MQ=255)
                                                                                                                                                 aaaCGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAAt   >  2:536102/1‑150 (MQ=255)
                                                                                                                                                 aaaCGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAAt   >  2:1404492/1‑150 (MQ=255)
                                                                                                                                                  aaCGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCACCATCGCATCAGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCGCTTCAGCCATCTATTTGGGAGCAAACAAtt  >  1:795288/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGTTCCGTGCTGGGGCTGATGGCATTTTTCTACTTCTTCGCCCGTTTGACGCTGTTTTGCGCGGCGTGGATTGCCACCGCCGAATATAAAGACGACCCGCGAATGCCGGGGAAAACGCAGCCTTAAAATAGGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGATTTTATTCGGCCTGGCTCCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATT  >  NC_000913/3674250‑3674545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: