Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 475,971 C→G 10.5% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913475,9710CG10.5% 314.0 / 24.8 105intergenic (+20/+11)ybaY/ybaZouter membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
Reads supporting (aligned to +/- strand):  ref base C (48/45);  new base G (5/6);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-01

Only 100 of 105 total aligned reads displayed.

TTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCG  >  NC_000913/475826‑476119
                                                                                                                                                 |                                                                                                                                                    
ttGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCgg                                                                                                                                                  <  2:829260/150‑1 (MQ=255)
   gTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGgaga                                                                                                                                               >  2:421617/1‑150 (MQ=255)
   gTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGgaga                                                                                                                                               >  2:517641/1‑150 (MQ=255)
     gCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAgg                                                                                                                                             <  2:1329094/150‑1 (MQ=255)
      ccGGTACAGCAACCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAggg                                                                                                                                            <  1:210092/150‑1 (MQ=255)
      ccGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                            <  2:1095007/150‑1 (MQ=255)
      ccGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                            <  2:1543697/150‑1 (MQ=255)
      ccGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAggg                                                                                                                                            <  1:1554626/150‑1 (MQ=255)
       cGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGt                                                                                                                                           <  1:503502/150‑1 (MQ=255)
       cGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGt                                                                                                                                           <  2:1367269/150‑1 (MQ=255)
       cGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGt                                                                                                                                           <  1:525202/150‑1 (MQ=255)
       cGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCGCCTACGCAATATTAAGCGCCGTTAACCCTCACCCCCCGGAGAGGGt                                                                                                                                           >  2:110826/1‑150 (MQ=255)
       cGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTAGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGt                                                                                                                                           <  1:412888/150‑1 (MQ=255)
         gTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCATCGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTta                                                                                                                                         <  1:1629370/150‑1 (MQ=255)
              gCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTagtagt                                                                                                                                    >  2:637508/1‑150 (MQ=255)
              gCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGGTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTagtagt                                                                                                                                    >  1:1533768/1‑150 (MQ=255)
              gCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGAACCTTCGACTTCCCCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTTAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTGagtagt                                                                                                                                    >  1:647826/1‑150 (MQ=255)
                aaaCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACGCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTc                                                                                                                                  >  1:1311204/1‑150 (MQ=255)
                    cgccgTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGc                                                                                                                              <  1:790160/150‑1 (MQ=255)
                    cgccgTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGc                                                                                                                              <  1:648439/150‑1 (MQ=255)
                    cgccgTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGc                                                                                                                              <  2:501494/150‑1 (MQ=255)
                     gccgTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCg                                                                                                                             <  2:780422/150‑1 (MQ=255)
                       cgTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTTGTTCCAGCGAt                                                                                                                           >  2:550842/1‑150 (MQ=255)
                           ccGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACg                                                                                                                       <  1:38235/150‑1 (MQ=255)
                               ttCATGCCAGCTGTGTCGCAACGACTACCGTACCTTCTACTTCACCATGTTCGGTGAATCCGTCTTCGGCAGTTGCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGTTATCGCTgg                                                                                                                   <  2:167820/150‑2 (MQ=255)
                                     ccAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCg                                                                                                             >  1:1653220/1‑150 (MQ=255)
                                      cAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGa                                                                                                            <  1:1624827/150‑1 (MQ=255)
                                                gcAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGcg                                                                                                  >  1:1087130/1‑149 (MQ=255)
                                                 cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCtt                                                                                                 >  2:939631/1‑150 (MQ=255)
                                                    cGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTccc                                                                                              >  1:434089/1‑150 (MQ=255)
                                                     gACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCg                                                                                             <  2:191940/150‑1 (MQ=255)
                                                      aCTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGa                                                                                            >  2:637432/1‑150 (MQ=255)
                                                        tACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATa                                                                                          <  1:1502074/150‑1 (MQ=255)
                                                          ccGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATAcc                                                                                        >  1:964767/1‑150 (MQ=255)
                                                           cGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCTCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAATTCGATTTGCCCGCTTCCCGATACCa                                                                                       <  2:501985/150‑1 (MQ=255)
                                                                cTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGAGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGAAATTCGATTTGCCCTCTTCCCGAGACCATcacc                                                                                  >  1:457280/1‑149 (MQ=255)
                                                                  tCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACAcc                                                                                <  2:1376092/150‑1 (MQ=255)
                                                                  tCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACAcc                                                                                <  1:1510500/150‑1 (MQ=255)
                                                                   cGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCt                                                                               >  1:709032/1‑150 (MQ=255)
                                                                    gACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCtt                                                                              >  2:547344/1‑150 (MQ=255)
                                                                      cTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt                                                                            >  2:940298/1‑150 (MQ=255)
                                                                      cTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt                                                                            >  2:1707040/1‑150 (MQ=255)
                                                                      cTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt                                                                            >  1:621445/1‑150 (MQ=255)
                                                                        tCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCATGTCGATTGGCCCTCTTGCCGCTACCATCACACCTTCTGc                                                                          >  2:234875/1‑150 (MQ=255)
                                                                        tCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGc                                                                          >  1:700047/1‑150 (MQ=255)
                                                                        tCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGc                                                                          >  1:11562/1‑150 (MQ=255)
                                                                         cACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGcc                                                                         <  2:651742/150‑1 (MQ=255)
                                                                         cACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGcc                                                                         <  1:889668/150‑1 (MQ=255)
                                                                           ccAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAg                                                                       >  1:389316/1‑150 (MQ=255)
                                                                            cAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGt                                                                      <  2:807657/150‑1 (MQ=255)
                                                                            cAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCAAACCTTCTGCCAGt                                                                      <  2:1686768/150‑1 (MQ=255)
                                                                                   ggTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCACGATACCATCACACCTTCTGCCAGTAATGCCt                                                               >  1:112492/1‑150 (MQ=255)
                                                                                      gAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCtt                                                                              >  2:789851/1‑132 (MQ=255)
                                                                                      gAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCtt                                                                              <  1:789851/132‑1 (MQ=255)
                                                                                        aTCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGc                                                          <  1:160981/150‑1 (MQ=255)
                                                                                           cGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGa                                                       >  1:1043381/1‑150 (MQ=255)
                                                                                            gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGAc                                                      >  1:1657596/1‑150 (MQ=255)
                                                                                            gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTAAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGAc                                                      <  1:110826/150‑1 (MQ=255)
                                                                                               ttCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCt                                                   <  2:782145/150‑1 (MQ=255)
                                                                                                tCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGTGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGCTACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTg                                                  >  1:103145/1‑150 (MQ=255)
                                                                                                 cagCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGt                                                 >  2:1378852/3‑150 (MQ=255)
                                                                                                 cGGCAGTTCCAGCTGCTACGCATTATTAAGCGACGTTAACCCTTTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTTTCGCTGACGCTGt                                                 <  1:1138542/150‑1 (MQ=255)
                                                                                                 cGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAACTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCTCTGACGCTGt                                                 >  1:150530/1‑150 (MQ=255)
                                                                                                 cGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCACCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGt                                                 >  2:1131125/1‑150 (MQ=255)
                                                                                                 cGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCACCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGt                                                 >  2:36357/1‑150 (MQ=255)
                                                                                                  ggCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTa                                                <  1:1576523/150‑1 (MQ=255)
                                                                                                      gTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtc                                            >  1:1468143/1‑150 (MQ=255)
                                                                                                       ttCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtcc                                           >  2:1538672/1‑150 (MQ=255)
                                                                                                        tCCAGCTCCTACGCATTATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATTCCCTCACACCTTCTGCCAGTACTGGCTGTCGCTGCCGCTGGAACtccg                                          >  1:252332/1‑150 (MQ=255)
                                                                                                        tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCGCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccg                                          <  2:878213/150‑1 (MQ=255)
                                                                                                         ccAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccgg                                         <  1:637432/150‑1 (MQ=255)
                                                                                                            gCTCCTTCGCAATAGAAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCCGCGATAACGCTGCAAGTCGATTTGCCAGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtcc                                      <  2:1042148/150‑1 (MQ=255)
                                                                                                            gCTCCTACGCAATATTAAGCGACGTTTAGCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGTTAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtcc                                      <  2:543274/150‑1 (MQ=255)
                                                                                                            gCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtcc                                      <  1:1066147/150‑1 (MQ=255)
                                                                                                            gCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtcc                                      <  1:853944/150‑1 (MQ=255)
                                                                                                                  aCGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAg                                >  2:956168/1‑150 (MQ=255)
                                                                                                                  aCGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGGAAATCCGGTCCGGTTAg                                >  1:1221191/1‑150 (MQ=255)
                                                                                                                            aaGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTTGTAGTTCCCGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCCCACCTTCTGCCAGTCATGCCTGTCGCTGCCGCTGTGAATCCGGTCCGGTTAGCGAAATtgtg                      >  2:1118106/1‑150 (MQ=255)
                                                                                                                            aaGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATtgtg                      <  2:1653220/150‑1 (MQ=255)
                                                                                                                            aaGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCAGCTTCCCGATCCAATCACAACTTCTGCAAGAATTGCCTGTCGCTGCCGCTATAAATCAGGTACTGATAGCGAAATtgtg                      >  1:918596/1‑150 (MQ=255)
                                                                                                                             aGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACCCAGCAAGTCTATTTTCCAGCTTCCCGATACCATCACACCTTCTTCCAGTAATGCCTGTCGCAGACGCAGTAAATCCGGTCCGGTAAGCGAAATtgttc                     >  2:1419465/1‑148 (MQ=255)
                                                                                                                             aGCGACGTGAACCCTCTCCGACCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTTCCGATACCTTCTCACCTGCTGCCAGTCTTGCCTGGCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGc                     <  1:277491/150‑1 (MQ=255)
                                                                                                                             aGCGACGGTCACCCTCTCCGCCCGTAGAGGGTTAGTAGTTCCAGCGCTAACGCTGCAAGTCGATTCGCCCGCTTCCCGATACAATCACACCTTCTGCCAGTAATGCCTTTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGc                     <  1:281297/150‑1 (MQ=255)
                                                                                                                              gCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACGCCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGcc                    >  1:1454647/1‑150 (MQ=255)
                                                                                                                              gCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAATTTGTGcc                    >  1:182854/1‑150 (MQ=255)
                                                                                                                                  cgatAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTgg                <  1:100569/147‑1 (MQ=255)
                                                                                                                                   gTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGGCTGTCGCTGACGCTGTATATCCGGTCCGGTTAGCGAAATTGTGCCGTGGc               >  2:1523000/1‑150 (MQ=255)
                                                                                                                                    ttAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCCGTAATGCCTGTCGCTGAAGCTGTAAATACGGTCCGGTTAGCGAAATTGTGCCGTGGCg              >  1:1324856/1‑150 (MQ=255)
                                                                                                                                    ttAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCg              <  1:462125/150‑1 (MQ=255)
                                                                                                                                    ctAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTGCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTATATCCGGTCCGGTGAGCGAAATTGTGCCGTGGCg              >  1:918811/2‑150 (MQ=255)
                                                                                                                                     tAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAGTTGCCCGCTTCCCGGTACCATCACACCTTCTGCCAGTAATGCCTGACGCTGACGCTGTAAATCTGGTCCGGTTAGCGAAATAGTTCCGTGTCGa             >  1:277488/1‑150 (MQ=255)
                                                                                                                                      aaCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAt            >  2:389150/1‑150 (MQ=255)
                                                                                                                                      aaCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCAGGTCCGGTGAGCGATATTGTGCCGTGGCGAt            >  2:211057/1‑150 (MQ=255)
                                                                                                                                       aCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCGAGTCGATTTGCCCGCTTCCCGCTACCATCACACCTTCTCCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAtt           >  2:839896/1‑150 (MQ=255)
                                                                                                                                       aCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAtt           >  2:493372/1‑150 (MQ=255)
                                                                                                                                       aCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAtt           >  2:486105/1‑150 (MQ=255)
                                                                                                                                         ccTCTCCGCCGCGAGAGTGTTAGTAGTGCCAGCGATAACGCTGCAAGTCGATTTGCCCGCGTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTGGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTaa         <  1:1176821/150‑1 (MQ=255)
                                                                                                                                          ctctCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTGGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAac        <  1:826275/150‑1 (MQ=255)
                                                                                                                                               cGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCAccc   <  2:709032/150‑1 (MQ=255)
                                                                                                                                                gCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCg  <  2:1152630/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCG  >  NC_000913/475826‑476119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: