Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,676,271 A→T 6.5% intergenic (+163/+19) yhjE → / ← yhjG putative MFS transporter; membrane protein/putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,676,2710AT 6.5% 492.9 / 15.6 166intergenic (+163/+19)yhjE/yhjGputative MFS transporter; membrane protein/putative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base A (83/72);  new base T (6/5);  total (89/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 167 total aligned reads displayed.

TGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAA  >  NC_000913/3676124‑3676420
                                                                                                                                                   |                                                                                                                                                     
 gATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGAc                                                                                                                                                    >  1:1261289/1‑150 (MQ=255)
  aTGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACa                                                                                                                                                   >  1:1668039/1‑150 (MQ=255)
      gcTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTAAAAAAACACACATTTCCAACAATAAAAATATTTTTAATTTACCGATGTATGACAtacc                                                                                                                                               >  1:1578400/1‑147 (MQ=18)
       cTACGCTTATCGGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCgg                                                                                                                                              >  1:1382055/1‑150 (MQ=255)
       cTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCgg                                                                                                                                              <  1:1518511/150‑1 (MQ=255)
        tACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAATTATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATCCGGc                                                                                                                                             <  1:493084/150‑1 (MQ=255)
          cGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCaa                                                                                                                                           >  1:1121085/1‑150 (MQ=255)
           gCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATCCGGCAAt                                                                                                                                          <  1:1245589/150‑1 (MQ=255)
           gCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATCCGGCAAt                                                                                                                                          <  2:604209/150‑1 (MQ=255)
            cTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAtt                                                                                                                                         >  2:982842/1‑150 (MQ=255)
                tCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATCCGGCAATTAaat                                                                                                                                     >  1:534520‑M2/1‑147 (MQ=255)
                tCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatca                                                                                                                                     <  1:1534740/150‑1 (MQ=255)
                tCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatca                                                                                                                                     >  1:919837/1‑150 (MQ=255)
                    gCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCAc                                                                                                                                 >  2:373803/1‑150 (MQ=255)
                     ccTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCAct                                                                                                                                >  2:733004/1‑150 (MQ=255)
                               cATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTg                                                                                                                      >  1:782993/1‑150 (MQ=255)
                               cATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTg                                                                                                                      <  1:482298/150‑1 (MQ=255)
                                 tACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCg                                                                                                                    >  1:526411/1‑150 (MQ=255)
                                  aCAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGa                                                                                                                   >  2:329037/1‑150 (MQ=255)
                                        tatTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACaaaaa                                                                                                             >  2:843251/1‑149 (MQ=255)
                                          ttGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGTATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCg                                                                                                           <  2:1350402/150‑1 (MQ=255)
                                              aTTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCg                                                                                                       <  1:1265168/150‑1 (MQ=255)
                                               ttCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGa                                                                                                      >  2:1358508/1‑150 (MQ=255)
                                                tCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGac                                                                                                     <  2:1529356/150‑1 (MQ=255)
                                                tCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCAGGCAATTATCATCACTTCTTCATCTGCGACTAAATCGTCCGac                                                                                                     >  2:344900/1‑150 (MQ=255)
                                                 cGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGaca                                                                                                    >  2:1620131/1‑150 (MQ=255)
                                                  gCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGacac                                                                                                   >  1:643456/1‑150 (MQ=255)
                                                           ttcatgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGcc                                                                                          >  2:556700/6‑150 (MQ=38)
                                                           tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCCCTTCTTCATCTGCGACAAAATTGTCCGACACTGATTAGcc                                                                                          >  2:277855/1‑150 (MQ=255)
                                                           tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGcc                                                                                          <  1:1052486/150‑1 (MQ=255)
                                                           tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGcc                                                                                          <  1:888061/150‑1 (MQ=255)
                                                              aGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGGCAAAATCGTCCGACACTGATTAGCCTcc                                                                                       <  1:1144630/150‑1 (MQ=255)
                                                              aGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTcc                                                                                       <  2:167959/150‑1 (MQ=255)
                                                               tgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTccc                                                                                      >  1:751483/2‑150 (MQ=39)
                                                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTccc                                                                                      <  2:940782/150‑1 (MQ=255)
                                                                gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTcccc                                                                                     <  1:1488303/150‑1 (MQ=255)
                                                                    gATATGGCGTTCACTCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCg                                                                                 <  1:221242/150‑1 (MQ=255)
                                                                    gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCg                                                                                 <  2:848878/150‑1 (MQ=255)
                                                                    gATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACAATTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAAACGTCCGACACTGATTAGCCTCCCCTTct                                                                                 >  2:436261/1‑149 (MQ=255)
                                                                       aaGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGaa                                                                              >  2:1571893/1‑150 (MQ=255)
                                                                         ggCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAgg                                                                            >  2:1387632/1‑150 (MQ=255)
                                                                           cGTTCACGCCGCATCCGGCATGAACAAAGCACTCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTg                                                                          <  2:781854/150‑1 (MQ=255)
                                                                           cGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTg                                                                          >  2:1185667/1‑150 (MQ=255)
                                                                           cGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTg                                                                          <  2:1201484/150‑1 (MQ=255)
                                                                           cGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTg                                                                          >  2:1249936/1‑150 (MQ=255)
                                                                           cGTTCACGCCACATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTg                                                                          <  2:1525635/150‑1 (MQ=255)
                                                                            gTTCATGCCGGATGCGGCGTGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTga                                                                         >  1:1580173/1‑150 (MQ=255)
                                                                             ttCACGCCGCATCCGGCATGAACAAAGCACTCTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTgag                                                                        <  1:1245066/150‑1 (MQ=255)
                                                                             ttCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTgag                                                                        <  2:606831/150‑1 (MQ=255)
                                                                             ttCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTgag                                                                        <  2:1325713/150‑1 (MQ=255)
                                                                               aaCGCCGCATCCGGCATGAACAAAGCTCACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCTGTAGGTGAGAt                                                                      <  2:362016/149‑1 (MQ=255)
                                                                                aCGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAACTCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATc                                                                     >  1:454648/1‑150 (MQ=255)
                                                                                aCGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATc                                                                     >  1:315361/1‑150 (MQ=255)
                                                                                 cgccgcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGCTCa                                                                    >  2:1047937/1‑150 (MQ=255)
                                                                                  gccgcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTAaacagatttagattgttggcaaagtgtgctttgttcatgccggatgaggcgtgaacgccttatccggcc                                                                   <  2:1578400‑M2/150‑70 (MQ=255)
                                                                                  gccgcATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAg                                                                   >  1:527274/1‑150 (MQ=255)
                                                                                      cATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATACGGCAATTAaacagatttagattgttggcaaagtgtgctttgttcatgccggatgcggcgtgaacgccttatccggccttca                                                               <  1:1428778‑M2/150‑74 (MQ=255)
                                                                                         ccGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGt                                                            <  1:997409/150‑1 (MQ=255)
                                                                                            gCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTAaacagatttagattgttggcaaagtgtgctttgttcatgccggatgcggcgtgaacgccttatccggcctacaaatcct                                                         <  2:910502‑M2/150‑80 (MQ=255)
                                                                                            gCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAAc                                                         <  1:1173631/150‑1 (MQ=255)
                                                                                             cATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAAcg                                                        >  2:1235336/1‑150 (MQ=255)
                                                                                              aTGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAAcgc                                                       <  1:814444/150‑1 (MQ=255)
                                                                                               tGAACAAAGCACACTTTGCAAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGCCACTTATCATCACTTCTTCATCTTCGACAAAATCGTCCGGCCCTGATAATCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAAcgca                                                      >  1:1136020/1‑149 (MQ=255)
                                                                                                       gCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCgg                                              >  2:1300319/1‑150 (MQ=255)
                                                                                                          cacTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGgtgt                                           >  2:1210217/1‑150 (MQ=255)
                                                                                                          aacTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGgtgt                                           <  2:482777/149‑1 (MQ=255)
                                                                                                           acTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGtt                                          <  2:1127871/150‑1 (MQ=255)
                                                                                                            cTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTa                                         >  2:1133633/1‑150 (MQ=255)
                                                                                                            cTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTa                                         >  1:250249/1‑150 (MQ=255)
                                                                                                               tGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCa                                      <  1:1081001/150‑1 (MQ=255)
                                                                                                                 ccAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGc                                    >  1:1185963/1‑150 (MQ=255)
                                                                                                                     caaTCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTg                                >  2:859059/1‑150 (MQ=255)
                                                                                                                       aTCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTg                                                                          <  2:757309/106‑1 (MQ=255)
                                                                                                                       aTCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTg                                                                          >  1:757309/1‑106 (MQ=255)
                                                                                                                          tAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGt                           <  1:1696691/150‑1 (MQ=255)
                                                                                                                            aaTCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGc                         <  1:433577/150‑1 (MQ=255)
                                                                                                                             aTCTGTTTAATTGCCTGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCAGCGACAAAATCGTCCGACACTGATTAGCCTCCCCGTCGGAAGGTGAGATCAGTGCCAGAAACTCCGCCGCCGGTGTTACCAGCGTTGCCAGTGcc                        <  1:464075/150‑1 (MQ=255)
                                                                                                                               cTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGTGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgc                      <  1:1566915/150‑1 (MQ=255)
                                                                                                                               cTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgc                      <  1:1408281/150‑1 (MQ=255)
                                                                                                                               cTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgc                      <  2:315361/150‑1 (MQ=255)
                                                                                                                                tGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgca                     <  1:481156/150‑1 (MQ=255)
                                                                                                                                tGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgca                     <  2:1225013/150‑1 (MQ=255)
                                                                                                                                tGTTTAAATGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgca                     <  1:605639/150‑1 (MQ=255)
                                                                                                                                 gTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgcag                    <  1:733004/150‑1 (MQ=255)
                                                                                                                                  tttAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgcagc                   <  1:372323/150‑1 (MQ=255)
                                                                                                                                   ttAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgcagca                  <  1:1249936/150‑1 (MQ=255)
                                                                                                                                   ttAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCgcagca                  <  2:1138797/150‑1 (MQ=255)
                                                                                                                                     aaTTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAAc                <  2:919837/150‑1 (MQ=255)
                                                                                                                                     aaTTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAAc                <  1:1358508/150‑1 (MQ=255)
                                                                                                                                      aTTGCCGGATGTCTGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACa               >  2:1366310/1‑150 (MQ=255)
                                                                                                                                      aTTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCATTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACa               >  1:1031401/1‑150 (MQ=255)
                                                                                                                                         gCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGGGACAGcc            >  1:60621/1‑149 (MQ=255)
                                                                                                                                         gCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGca            >  1:1032991/1‑150 (MQ=255)
                                                                                                                                          ccGGATGTCTGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGcac           >  1:1650625/1‑150 (MQ=255)
                                                                                                                                          ccGGATGTCTGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGcac           >  1:1279080/1‑150 (MQ=255)
                                                                                                                                            ggATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCATCAGcacca         >  1:322866/1‑150 (MQ=255)
                                                                                                                                                gTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACggg     <  1:1496099/150‑1 (MQ=255)
                                                                                                                                                 tCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGc    <  1:1667928/150‑1 (MQ=255)
                                                                                                                                                  cAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCa   >  2:934759/1‑150 (MQ=255)
                                                                                                                                                   aGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGCTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCaa  <  1:212250/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGATCAGTGCCAGTAACGCCGCCGCCGGTGTTACCAGCGTTGCCAGTGCCGCAGCAACAGCACCACGGGCAA  >  NC_000913/3676124‑3676420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: