Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,700,418 G→A 7.1% intergenic (+217/‑145) rdlD → / → yhjV sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,700,4180GA 7.1% 388.8 / 12.5 126intergenic (+217/‑145)rdlD/yhjVsRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter
Reads supporting (aligned to +/- strand):  ref base G (54/63);  new base A (5/4);  total (59/67)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 130 total aligned reads displayed.

GCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATG  >  NC_000913/3700269‑3700565
                                                                                                                                                     |                                                                                                                                                   
gCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTg                                                                                                                                                     >  1:356761/1‑150 (MQ=255)
gCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTg                                                                                                                                                     >  1:750013/1‑150 (MQ=255)
gCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTg                                                                                                                                                     <  1:1385882/150‑1 (MQ=255)
   tGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAg                                                                                                                                                  <  1:1628787/150‑1 (MQ=255)
    gCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGa                                                                                                                                                 >  1:1199080/1‑150 (MQ=255)
     ccGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTGCCTCAGTGCGCGATGCCGTCCAGCGTAATTTCTTTATTTGCAATAGTTGCCGTTCATATCCGGGCTGACTCCCGCATGGTAGTGAAATGCGCAATTTTGTAgca                                                                                                                                                >  1:1355406/1‑148 (MQ=255)
          gATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcg                                                                                                                                           <  1:736011/150‑1 (MQ=255)
           aTTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgc                                                                                                                                          <  1:1364462/150‑1 (MQ=255)
            ttCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcc                                                                                                                                         >  1:1461711/1‑150 (MQ=255)
            ttCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGATTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTACAGACTTGTAGTGAAATGCGAAATTTTGTAGAATTGCGcc                                                                                                                                         >  1:1085605/1‑150 (MQ=255)
             tCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgcat                                                                                                                                        <  1:1192074/150‑3 (MQ=255)
               tACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgcattt                                                                                                                                      <  2:1002103/150‑5 (MQ=255)
                   aTTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTcgc                                                                                                                                  >  2:221373/1‑150 (MQ=255)
                      gCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAc                                                                                                                               >  1:632918/1‑150 (MQ=255)
                      gCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAc                                                                                                                               <  1:716034/150‑1 (MQ=255)
                             aCTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCa                                                                                                                        >  1:1538394/1‑150 (MQ=255)
                                  cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGaa                                                                                                                   >  1:1525291/1‑150 (MQ=255)
                                  cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGaa                                                                                                                   <  2:312158/150‑1 (MQ=255)
                                   aGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAAt                                                                                                                  >  2:1259942/1‑150 (MQ=255)
                                    gCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATc                                                                                                                 >  2:646660/1‑150 (MQ=255)
                                         cGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTg                                                                                                            <  1:984550/150‑1 (MQ=255)
                                          ggCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGc                                                                                                           >  2:431689/1‑150 (MQ=255)
                                          ggCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGc                                                                                                           >  2:756438/1‑150 (MQ=255)
                                               tCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAt                                                                                                      <  2:1351327/150‑1 (MQ=255)
                                                cTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAtt                                                                                                     <  1:756438/150‑1 (MQ=255)
                                                 ttCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGTGCTGACTCCAGACTGTTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttt                                                                                                    <  2:202174/150‑1 (MQ=255)
                                                 ttCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCTCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttt                                                                                                    >  1:1369123/1‑150 (MQ=255)
                                                   ccTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttttt                                                                                                  >  1:1129833/1‑150 (MQ=255)
                                                     tCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTa                                                                                                <  1:438700/150‑1 (MQ=255)
                                                      cATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCTCCAGTGAATCTGTTGCCTCATTTTTTAc                                                                                               >  1:60200/1‑150 (MQ=255)
                                                      cATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTAc                                                                                               >  2:520747/1‑150 (MQ=255)
                                                       aTTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACa                                                                                              >  1:907921/1‑150 (MQ=255)
                                                       aTTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACa                                                                                              >  1:1609731/1‑150 (MQ=255)
                                                            gcgATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTg                                                                                         <  1:759777/150‑1 (MQ=255)
                                                             cgATGCCTTCCAGCTTAATTTCATTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTCCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGc                                                                                        <  1:1649507/150‑1 (MQ=255)
                                                               aTGCCTTCCAGCTTAAATTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGt                                                                                      >  2:169763/1‑150 (MQ=255)
                                                                tGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGNCTCCTCNCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTa                                                                                     <  1:1323437/150‑1 (MQ=255)
                                                                tGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTa                                                                                     <  1:1722154/150‑1 (MQ=255)
                                                                tGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTa                                                                                     >  2:17066/1‑150 (MQ=255)
                                                                    ttCCAGCTTAATTTCTTTATTTTCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCATTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCg                                                                                 <  1:51582/150‑1 (MQ=255)
                                                                     tCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGa                                                                                <  2:823263/150‑1 (MQ=255)
                                                                        aGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACtt                                                                             >  1:771110/1‑150 (MQ=255)
                                                                        aGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCAAGACTGGTAGTGAAATGCGAAATTTTGTAGAATTGCGCATCCTCGCGACCTCACCAGTGAATCTGTTGACTCATTTTTTAAATACTGAGTATTCGACtt                                                                             >  1:1211189/1‑150 (MQ=255)
                                                                         gCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTtc                                                                            <  2:356761/150‑1 (MQ=255)
                                                                          cTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTtct                                                                           >  1:412058/1‑150 (MQ=255)
                                                                            tAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                                                                         >  1:898154/1‑150 (MQ=255)
                                                                            tAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                                                                         <  2:803450/150‑1 (MQ=255)
                                                                            tAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                                                                         >  1:904268/1‑150 (MQ=255)
                                                                             aaTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCa                                                                        <  2:464884/150‑1 (MQ=255)
                                                                              aTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcct                                                                                                                                        <  2:815548/85‑1 (MQ=255)
                                                                              aTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcct                                                                                                                                        >  1:815548/1‑85 (MQ=255)
                                                                               tttCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTATTGAAATGCTCAATTTTGCAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCAcc                                                                      <  1:779078/150‑1 (MQ=255)
                                                                                   tttATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGtt                                                                  >  1:399789/1‑150 (MQ=255)
                                                                                    ttATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTg                                                                 >  2:205993/1‑150 (MQ=255)
                                                                                      aTTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTgcg                                                               >  1:1144155/1‑150 (MQ=255)
                                                                                         tGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCaa                                                            >  1:145081/1‑150 (MQ=255)
                                                                                         tGCAATAGTTGCAGTTCATAACCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGCCCTCCCCAGTGAATCTGTAGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACATGTTGCGCaa                                                            >  2:1319461/1‑150 (MQ=255)
                                                                                          gCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAag                                                           >  2:907152/1‑150 (MQ=255)
                                                                                           cAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAaga                                                          <  1:463679/150‑1 (MQ=255)
                                                                                            aaTAGTTGCAGTTCATATCCGGGCTGACTCCTGACTGGTTGTGAAATGCGCAATTTTGTAGATTTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACTTACTGCGTATTCGACTTCTCCACCTGTTGCGCAagag                                                         <  2:793847/150‑1 (MQ=255)
                                                                                            aaTAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAagag                                                         <  1:1423699/150‑1 (MQ=255)
                                                                                            aaTAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAagag                                                         <  2:386560/150‑1 (MQ=255)
                                                                                             aTAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGCAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGAGTATTCGACTTCTCCACCTGTTGCGCAAgaga                                                        >  2:834801/1‑150 (MQ=255)
                                                                                                gTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGACTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAAc                                                     >  1:398259/1‑150 (MQ=255)
                                                                                                    taGTTCATATCCGTGCTGACTACAGACTGGTAGTGAAATGCGCTATTTTGTAGAATTGCGACTCCTCGGGACCGCCCCAGTGAATCTGTTGCCTCATTTTTTACATACAGGGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTggg                                                 <  2:721101/149‑1 (MQ=255)
                                                                                                    cAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTggg                                                 >  2:363941/1‑150 (MQ=255)
                                                                                                    cAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTggg                                                 <  2:1057562/150‑1 (MQ=255)
                                                                                                    cAGTTCATATCCGGCCTGACTCCACACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCATCGCGACCTCCGCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTggg                                                 <  2:106612/150‑1 (MQ=255)
                                                                                                      gctCATAACCGGGCTGACTCCAGCCTGGTAGTGATATGCGCAATTTTGTACAATTGCGCCTCCTCGCGACCTCCCCCGTGAATCTGTCGCCTCATTTTTTACATACTGCGAATTCGACTTCTCCCCCTGCTGCGCAAGAGAAACTGGGtt                                               <  2:260508/148‑1 (MQ=255)
                                                                                                      gTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGtt                                               <  2:705019/150‑1 (MQ=255)
                                                                                                      gTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGtt                                               >  2:607843/1‑150 (MQ=255)
                                                                                                      gTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGtt                                               <  1:874642/150‑1 (MQ=255)
                                                                                                       ttCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGttt                                              <  2:771110/150‑1 (MQ=255)
                                                                                                           tatCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTc                                          <  1:703755/150‑1 (MQ=255)
                                                                                                             tCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCAt                                        >  1:1529474/1‑150 (MQ=255)
                                                                                                              ccGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGTCCTCCCCAGTGAAGCTGGTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCAtt                                       <  1:708867/150‑1 (MQ=255)
                                                                                                              ccGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCAtt                                       <  1:1091593/150‑1 (MQ=255)
                                                                                                                 ggCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCAttttt                                    >  2:1248411/1‑150 (MQ=255)
                                                                                                                  gCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTg                                   <  2:802278/150‑1 (MQ=255)
                                                                                                                   cttaCTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGc                                  <  2:173196/147‑1 (MQ=255)
                                                                                                                     gACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGCCCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGa                                >  1:319375/1‑150 (MQ=255)
                                                                                                                      aCTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAg                               <  2:1121906/150‑1 (MQ=255)
                                                                                                                       cTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAgg                              >  1:522583/1‑150 (MQ=255)
                                                                                                                         ccAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGcc                            <  1:1259942/150‑1 (MQ=255)
                                                                                                                           aGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCTCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGa                          <  2:60200/150‑1 (MQ=255)
                                                                                                                             aCTGGTAGTGAAATTCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCCCTGAATCTTTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGAct                        <  2:811718/150‑1 (MQ=255)
                                                                                                                             aCTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGAct                        <  2:1636410/150‑1 (MQ=255)
                                                                                                                             aCTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGAct                        <  2:1129833/150‑1 (MQ=255)
                                                                                                                                 gTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtctt                    <  2:1588988/150‑1 (MQ=255)
                                                                                                                                 gTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtctt                    <  1:1663907/150‑1 (MQ=255)
                                                                                                                                    gggAAATGCGCAATTTAGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttctttct                 <  2:672380/148‑1 (MQ=255)
                                                                                                                                          tGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCATGAGAAACTGGGTTTATTCAGTTTTGCGAGGCCGACTTCTTTCTGGACAg           >  2:1188114/1‑150 (MQ=255)
                                                                                                                                          tGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg           <  2:1638875/150‑1 (MQ=255)
                                                                                                                                          ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCGTCATTTTTTACATACTGCGTATTGGAGTTATCCACCTGTGGCGAATGAGATACGGGGGTTATTCATTTTTGCGCGGGGGACTTCTTTCTGGGCAg           >  2:1248280/2‑150 (MQ=255)
                                                                                                                                          ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg           >  1:779704/2‑150 (MQ=255)
                                                                                                                                          ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg           >  1:101352/2‑150 (MQ=255)
                                                                                                                                          ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg           >  2:1348384/2‑150 (MQ=255)
                                                                                                                                               aaTTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACtt      <  1:646660/150‑1 (MQ=255)
                                                                                                                                                 ttttGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTa    <  2:1352468/150‑1 (MQ=255)
                                                                                                                                                  tttGTAGAATTGCGCCGCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTAt   <  1:841270/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATG  >  NC_000913/3700269‑3700565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: