Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,700,421 G→A 7.3% intergenic (+220/‑142) rdlD → / → yhjV sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,700,4210GA 7.3% 382.5 / 11.4 125intergenic (+220/‑142)rdlD/yhjVsRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter
Reads supporting (aligned to +/- strand):  ref base G (54/61);  new base A (5/4);  total (59/66)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

Only 100 of 128 total aligned reads displayed.

TGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATGCAGCA  >  NC_000913/3700272‑3700570
                                                                                                                                                     |                                                                                                                                                     
tGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAg                                                                                                                                                       <  1:1628787/150‑1 (MQ=255)
 gCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGa                                                                                                                                                      >  1:1199080/1‑150 (MQ=255)
       gATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcg                                                                                                                                                <  1:736011/150‑1 (MQ=255)
        aTTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgc                                                                                                                                               <  1:1364462/150‑1 (MQ=255)
         ttCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcc                                                                                                                                              <  2:1326842/150‑1 (MQ=255)
         ttCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcc                                                                                                                                              <  2:1651749/150‑1 (MQ=255)
         ttCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcc                                                                                                                                              >  1:1461711/1‑150 (MQ=255)
         ttCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGATTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTACAGACTTGTAGTGAAATGCGAAATTTTGTAGAATTGCGcc                                                                                                                                              >  1:1085605/1‑150 (MQ=255)
          tCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgcat                                                                                                                                             <  1:1192074/150‑3 (MQ=255)
                 ttGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTcgcg                                                                                                                                      <  1:1482893/150‑1 (MQ=255)
                   gCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAc                                                                                                                                    <  1:716034/150‑1 (MQ=255)
                   gCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAc                                                                                                                                    >  1:632918/1‑150 (MQ=255)
                    cTTTTTACTGTCAGCACCCGGACATCTTCCTCATTGCGCGATGCCGTCCAGCTTAATTTCGTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGAGAcc                                                                                                                                   <  1:98844/150‑1 (MQ=255)
                        ttACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTccc                                                                                                                               >  1:802278/1‑150 (MQ=255)
                          aCTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCa                                                                                                                             >  1:1538394/1‑150 (MQ=255)
                           cTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAg                                                                                                                            >  2:205419/1‑150 (MQ=255)
                             gTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTg                                                                                                                          <  1:696028/150‑1 (MQ=255)
                               cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGaa                                                                                                                        >  1:1525291/1‑150 (MQ=255)
                               cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGaa                                                                                                                        <  2:312158/150‑1 (MQ=255)
                                aGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAAt                                                                                                                       >  2:1259942/1‑150 (MQ=255)
                                  cACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCt                                                                                                                     <  2:654720/150‑1 (MQ=255)
                                   aCCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTg                                                                                                                    >  1:430543/1‑150 (MQ=255)
                                      cGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTg                                                                                                                 <  1:984550/150‑1 (MQ=255)
                                       ggCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGc                                                                                                                >  2:756438/1‑150 (MQ=255)
                                       ggCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGc                                                                                                                >  2:431689/1‑150 (MQ=255)
                                             cTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAtt                                                                                                          <  1:756438/150‑1 (MQ=255)
                                              ttCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGTGCTGACTCCAGACTGTTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttt                                                                                                         <  2:202174/150‑1 (MQ=255)
                                              ttCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCTCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttt                                                                                                         >  1:1369123/1‑150 (MQ=255)
                                               tCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAtttt                                                                                                        >  1:1588988/1‑150 (MQ=255)
                                                ccTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttttt                                                                                                       >  1:1129833/1‑150 (MQ=255)
                                                 cTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATTTCCGGGCTGACTCGAGCCTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGATTCTGGTGGCTCAgttttt                                                                                                      >  2:561144/1‑150 (MQ=255)
                                                  tCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTa                                                                                                     <  1:438700/150‑1 (MQ=255)
                                                   cATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCTCCAGTGAATCTGTTGCCTCATTTTTTAc                                                                                                    >  1:60200/1‑150 (MQ=255)
                                                    aTTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACa                                                                                                   >  1:1609731/1‑150 (MQ=255)
                                                    aTTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACa                                                                                                   >  1:907921/1‑150 (MQ=255)
                                                         gcgATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTg                                                                                              <  1:759777/150‑1 (MQ=255)
                                                            aTGCCTTCCAGCTTAAATTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGt                                                                                           >  2:169763/1‑150 (MQ=255)
                                                             tGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGNCTCCTCNCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTa                                                                                          <  1:1323437/150‑1 (MQ=255)
                                                             tGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTa                                                                                          >  2:17066/1‑150 (MQ=255)
                                                             tGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTa                                                                                          <  1:1330604/150‑1 (MQ=255)
                                                                 ttCCAGCTTAATTTCTTTATTTTCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCATTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCg                                                                                      <  1:51582/150‑1 (MQ=255)
                                                                  tCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGa                                                                                     <  2:823263/150‑1 (MQ=255)
                                                                     aGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACtt                                                                                  >  1:771110/1‑150 (MQ=255)
                                                                      gCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTtc                                                                                 <  2:356761/150‑1 (MQ=255)
                                                                         tAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                                                                              <  2:803450/150‑1 (MQ=255)
                                                                         tAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                                                                              >  1:904268/1‑150 (MQ=255)
                                                                          aaTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCa                                                                             <  2:464884/150‑1 (MQ=255)
                                                                           aTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcct                                                                                                                                             <  2:815548/85‑1 (MQ=255)
                                                                           aTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACAGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATATGTTGCGTCATTTTTTACATACTGCGTATTCGCCTTCTCCAc                                                                            >  1:884986/1‑150 (MQ=255)
                                                                            tttCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTATTGAAATGCTCAATTTTGCAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCAcc                                                                           <  1:779078/150‑1 (MQ=255)
                                                                                tttATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGtt                                                                       >  1:399789/1‑150 (MQ=255)
                                                                                 ttATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTg                                                                      >  2:205993/1‑150 (MQ=255)
                                                                                   aTTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTgcg                                                                    >  1:1144155/1‑150 (MQ=255)
                                                                                     ttGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCa                                                                  <  1:1210690/150‑1 (MQ=255)
                                                                                      tGCAATAGTTGCAGTTCATAACCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGCCCTCCCCAGTGAATCTGTAGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACATGTTGCGCaa                                                                 >  2:1319461/1‑150 (MQ=255)
                                                                                       gCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAag                                                                >  2:907152/1‑150 (MQ=255)
                                                                                        cAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAaga                                                               <  1:463679/150‑1 (MQ=255)
                                                                                         aaTAGTTGCAGTTCATATCCGGGCTGACTCCTGACTGGTTGTGAAATGCGCAATTTTGTAGATTTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACTTACTGCGTATTCGACTTCTCCACCTGTTGCGCAagag                                                              <  2:793847/150‑1 (MQ=255)
                                                                                         aaTAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAagag                                                              <  2:386560/150‑1 (MQ=255)
                                                                                         aaTAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAagag                                                              <  1:1423699/150‑1 (MQ=255)
                                                                                          aTAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGCAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGAGTATTCGACTTCTCCACCTGTTGCGCAAgaga                                                             >  2:834801/1‑150 (MQ=255)
                                                                                             gTTGCAGTTCATATCCGGGCTGACTCCAGCCTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAAc                                                          >  1:564870/1‑150 (MQ=255)
                                                                                             gTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGACTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAAc                                                          >  1:398259/1‑150 (MQ=255)
                                                                                                gCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTgg                                                       >  2:1547357/1‑150 (MQ=255)
                                                                                                 taGTTCATATCCGTGCTGACTACAGACTGGTAGTGAAATGCGCTATTTTGTAGAATTGCGACTCCTCGGGACCGCCCCAGTGAATCTGTTGCCTCATTTTTTACATACAGGGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTggg                                                      <  2:721101/149‑1 (MQ=255)
                                                                                                 cAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTggg                                                      <  2:1057562/150‑1 (MQ=255)
                                                                                                 cAGTTCATATCCGGCCTGACTCCACACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCATCGCGACCTCCGCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTggg                                                      <  2:106612/150‑1 (MQ=255)
                                                                                                   gctCATAACCGGGCTGACTCCAGCCTGGTAGTGATATGCGCAATTTTGTACAATTGCGCCTCCTCGCGACCTCCCCCGTGAATCTGTCGCCTCATTTTTTACATACTGCGAATTCGACTTCTCCCCCTGCTGCGCAAGAGAAACTGGGtt                                                    <  2:260508/148‑1 (MQ=255)
                                                                                                   gTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGtt                                                    <  2:705019/150‑1 (MQ=255)
                                                                                                   gTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGtt                                                    >  2:607843/1‑150 (MQ=255)
                                                                                                   gTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGtt                                                    <  1:874642/150‑1 (MQ=255)
                                                                                                    ttCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGttt                                                   <  2:771110/150‑1 (MQ=255)
                                                                                                      cATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTAt                                                 >  2:1105762/1‑150 (MQ=255)
                                                                                                       atatCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTAtt                                                <  2:377038/150‑1 (MQ=255)
                                                                                                        tatCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTc                                               <  1:703755/150‑1 (MQ=255)
                                                                                                           ccGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGTCCTCCCCAGTGAAGCTGGTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCAtt                                            <  1:708867/150‑1 (MQ=255)
                                                                                                           ccGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCAtt                                            <  1:1091593/150‑1 (MQ=255)
                                                                                                                  gACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGCCCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGa                                     >  1:319375/1‑150 (MQ=255)
                                                                                                                   aCTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAg                                    <  2:1121906/150‑1 (MQ=255)
                                                                                                                    cTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAgg                                   <  1:1403878/150‑1 (MQ=255)
                                                                                                                      ccAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGcc                                 <  2:295470/150‑1 (MQ=255)
                                                                                                                      ccAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGcc                                 <  1:1259942/150‑1 (MQ=255)
                                                                                                                        aGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCTCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGa                               <  2:60200/150‑1 (MQ=255)
                                                                                                                          aCTGGTAGTGAAATTCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCCCTGAATCTTTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGAct                             <  2:811718/150‑1 (MQ=255)
                                                                                                                              gTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtctt                         <  1:1663907/150‑1 (MQ=255)
                                                                                                                              gTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtctt                         <  2:1588988/150‑1 (MQ=255)
                                                                                                                                aGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttctttc                       >  1:377405/1‑150 (MQ=255)
                                                                                                                                 gggAAATGCGCAATTTAGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttctttct                      <  2:672380/148‑1 (MQ=255)
                                                                                                                                 gTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttctttct                      <  2:802380/150‑1 (MQ=255)
                                                                                                                                  tGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTg                     <  1:169763/150‑1 (MQ=255)
                                                                                                                                       tGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCATGAGAAACTGGGTTTATTCAGTTTTGCGAGGCCGACTTCTTTCTGGACAg                >  2:1188114/1‑150 (MQ=255)
                                                                                                                                       tGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg                <  2:1638875/150‑1 (MQ=255)
                                                                                                                                       ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCGTCATTTTTTACATACTGCGTATTGGAGTTATCCACCTGTGGCGAATGAGATACGGGGGTTATTCATTTTTGCGCGGGGGACTTCTTTCTGGGCAg                >  2:1248280/2‑150 (MQ=255)
                                                                                                                                       ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg                >  2:1348384/2‑150 (MQ=255)
                                                                                                                                       ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg                >  1:101352/2‑150 (MQ=255)
                                                                                                                                       ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg                >  1:779704/2‑150 (MQ=255)
                                                                                                                                              ttttGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTa         <  2:1352468/150‑1 (MQ=255)
                                                                                                                                               tttGTAGAATTGCGCCGCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTAt        <  1:841270/150‑1 (MQ=255)
                                                                                                                                                  gTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCCCCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACTGGACTTTATgaa     >  2:1246744/1‑148 (MQ=255)
                                                                                                                                                     gAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGCCTTCTTTCTGGACAGGCCTTTATgcagca  >  1:1709965/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATGCAGCA  >  NC_000913/3700272‑3700570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: