Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,710,635 G→T 7.1% intergenic (‑160/+164) eptB ← / ← yhjX KDO phosphoethanolamine transferase, Ca(2+)‑inducible/pyruvate‑inducible inner membrane protein, putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,710,6350GT 7.1% 332.2 / 3.8 126intergenic (‑160/+164)eptB/yhjXKDO phosphoethanolamine transferase, Ca(2+)‑inducible/pyruvate‑inducible inner membrane protein, putative transporter
Reads supporting (aligned to +/- strand):  ref base G (58/57);  new base T (8/3);  total (66/60)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

CCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCAGTA  >  NC_000913/3710486‑3710783
                                                                                                                                                     |                                                                                                                                                    
ccTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCATGCCCGGCAGGGTAAAATTTTTTAGGTAGTCAGATAAGTTATATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAg                                                                                                                                                      <  2:19387/150‑1 (MQ=255)
ccTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAg                                                                                                                                                      >  2:1706069/1‑150 (MQ=255)
 cTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGc                                                                                                                                                     <  2:756309/150‑1 (MQ=255)
 cTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGc                                                                                                                                                     <  2:1610410/150‑1 (MQ=255)
  tgtaCAAACAGGTGATAAAACCCTGCTTAGGTGCTGTTCAGGCCCGGCAGGTTTAAAGTGCGGAGGTAGTCAGATAAGTAATATGTACTCGCGCGTATACGTAGACCACAGCAAAGGGAATAAGTGTGGGGAAAGAGTGTGCATGAATca                                                                                                                                                    <  1:1237340/147‑1 (MQ=255)
   ggACAACCAGGTGATAACTCCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGcac                                                                                                                                                   <  1:603115/150‑1 (MQ=255)
     aCAAACAGGTGATAACACCCTGCTTAGTGGCGTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGATTAAGTAGACCACAGCAAAGGGAAAAAGTTTGGGGAAAGAGTGTGCATGAAGCACaa                                                                                                                                                 >  2:1597137/1‑150 (MQ=255)
       aaaCAGGTGATAACTACTTGATTAGTGGCTTTTCAGGACCGGAAGTGATAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGGGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGTGAAAGAGTGTGCATGAAGCACAAAt                                                                                                                                               <  1:285268/150‑1 (MQ=255)
       aaaCAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAAt                                                                                                                                               <  1:465530/150‑1 (MQ=255)
        aaCAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAAtt                                                                                                                                              >  2:850108/1‑150 (MQ=255)
          cAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTa                                                                                                                                            >  2:698367/1‑150 (MQ=255)
          cAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTa                                                                                                                                            >  1:1060790/1‑150 (MQ=255)
           aGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTAc                                                                                                                                           <  1:231256/150‑1 (MQ=255)
            ggTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACa                                                                                                                                          <  1:64940/150‑1 (MQ=255)
            ggTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACa                                                                                                                                          >  2:1215533/1‑150 (MQ=255)
             gTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACaa                                                                                                                                         >  1:376200/1‑150 (MQ=255)
               gATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGGGTCGGGAAAGAGTGGCCATGAAGCACAAAATTACaaaa                                                                                                                                       >  2:316252/1‑150 (MQ=255)
                aTAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGGCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACaaaaa                                                                                                                                      <  2:175017/150‑1 (MQ=255)
                  aaCACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATg                                                                                                                                    >  2:181720/1‑150 (MQ=255)
                   acacCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGc                                                                                                                                   >  2:482513/1‑150 (MQ=255)
                   acacCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGc                                                                                                                                   >  1:427060/1‑150 (MQ=255)
                     acCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCaa                                                                                                                                 <  2:1550717/150‑1 (MQ=255)
                        cTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGtt                                                                                                                              <  1:1637262/150‑1 (MQ=255)
                             tAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCTAATAAGTAGACCACAGCAAAGTGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaaa                                                                                                                         <  1:113989/150‑1 (MQ=255)
                             tAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaaa                                                                                                                         <  1:1579709/150‑1 (MQ=255)
                                   cTTTTCAGGCCCGGCAGGGAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGATTAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACt                                                                                                                  >  1:1557097/1‑150 (MQ=255)
                                       tCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTa                                                                                                               >  2:1412850/1‑150 (MQ=255)
                                        cAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAg                                                                                                              >  2:900603/1‑150 (MQ=255)
                                          ggCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCg                                                                                                            >  2:873127/1‑150 (MQ=255)
                                            cccGGCATGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGtt                                                                                                          <  1:456854/150‑1 (MQ=255)
                                            cccGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGtt                                                                                                          <  2:1600005/150‑1 (MQ=255)
                                            cccGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGtt                                                                                                          <  2:1505997/150‑1 (MQ=255)
                                               ggCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGAAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACa                                                                                                       >  1:1305782/1‑150 (MQ=255)
                                                 cAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACaaa                                                                                                     >  1:451648/1‑150 (MQ=255)
                                                  aGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACaaaa                                                                                                    >  2:1438942/1‑150 (MQ=255)
                                                   gggAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCAAAAATTTACAATAATGCAAGATTTAAAAGATACTTTAGCGGTACaaaaa                                                                                                   >  2:464722/1‑150 (MQ=255)
                                                    ggAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAagag                                                                                                                     <  1:1385777/131‑1 (MQ=255)
                                                    ggAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGAATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAg                                                                                                  >  2:582582/1‑150 (MQ=255)
                                                     gAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGc                                                                                                 >  2:239346/1‑150 (MQ=255)
                                                         aaGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTa                                                                                             >  2:133515/1‑150 (MQ=255)
                                                          aGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAATAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTaa                                                                                            <  2:1469736/150‑1 (MQ=255)
                                                              cGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAg                                                                                        <  2:1144691/150‑1 (MQ=255)
                                                               ggAGGTAGTCAGATAAGTAAAATATACACCCTCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTTCAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAgg                                                                                       <  2:98014/150‑1 (MQ=255)
                                                                    tAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAg                                                                                  >  1:1081510/1‑150 (MQ=255)
                                                                        cAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATTAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGATACTTTATCGTTACAAAAAGCAGTAAAAAGGGCCAGTTaa                                                                              >  2:836790/1‑150 (MQ=255)
                                                                         aGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAATCACAAATTTACAAAAATGCAAGTTATAAAAAAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAAt                                                                             >  2:1649402/1‑150 (MQ=255)
                                                                              aGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGcc                                                                        >  1:1537634/1‑150 (MQ=255)
                                                                              aGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGccc                                                                       >  2:1711403/1‑150 (MQ=255)
                                                                               gTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTTCATGAAGCACAAATTTACAAAAATCCAATTTAGAAAATATACATTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGccc                                                                       >  2:169691/1‑150 (MQ=255)
                                                                               gTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGccc                                                                       >  1:1452604/1‑150 (MQ=255)
                                                                               gTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGccc                                                                       <  2:1553514/150‑1 (MQ=255)
                                                                                tAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAATTATCTGGccct                                                                      <  2:1144622/150‑2 (MQ=255)
                                                                                   aaTCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAAt                                                                   >  2:474221/1‑150 (MQ=255)
                                                                                    aTCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAACAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAAtt                                                                  <  1:1106254/150‑1 (MQ=255)
                                                                                       tACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTa                                                               >  2:581390/1‑150 (MQ=255)
                                                                                        acacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTAc                                                              <  2:1345975/150‑1 (MQ=255)
                                                                                         cacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACg                                                             <  1:900603/150‑1 (MQ=255)
                                                                                         cacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACg                                                             >  1:97030/1‑150 (MQ=255)
                                                                                           cgcgcgAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTAGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCAAAATTTTACGAg                                                           >  2:61111/1‑150 (MQ=255)
                                                                                            gcgagAATAATTAGACCAAATCTAATGTAAAAAGTGTTGGTAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGt                                                          <  1:40452/150‑1 (MQ=255)
                                                                                                gAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCTTGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCAtt                                                      <  2:732568/150‑1 (MQ=255)
                                                                                                 aaTAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTg                                                     <  1:1706069/150‑1 (MQ=255)
                                                                                                     aGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTta                                                 <  1:1239405/150‑1 (MQ=255)
                                                                                                        tgACCACTGGTAAGGTAATAAGTGTGGGGATAGATTGTGCATGAAGCAAAAATTTAAATAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAATGCAGTAAAAAGGGCGAGATTATTGGCCCAAATTTTACGAGTCATTGTTTATAc                                              <  2:768614/149‑1 (MQ=255)
                                                                                                        aGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATCAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACATTAGCGTTACAAAAAGCAGTAAAAAGGGGCAGTTACTTGGCCCAAATTTTACGAGACATTGTTTATAc                                              >  2:1560741/1‑150 (MQ=255)
                                                                                                           ccACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGAATGAATCAAAAAATAACAATAATGCAAGTAAGAAAAAAAACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACAtt                                           >  2:776734/1‑150 (MQ=255)
                                                                                                            cacaGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTTAATCAATCACAAAATAACAAAAATCCAAATTTTAAAAAAAAATTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATATAACGAGTCATTGTTTATACAttt                                          >  2:774370/1‑150 (MQ=255)
                                                                                                               aGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGt                                       >  2:512520/1‑150 (MQ=255)
                                                                                                               aGCAAAGGGAAAAAGTGTAGGGAAAGAGTGTAAATAAATCAAAAAATTACAAAAATCCAAATTTTAAAAAATACAATAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGt                                       >  2:332077/1‑150 (MQ=255)
                                                                                                                tcAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGtt                                      <  1:219470/149‑1 (MQ=255)
                                                                                                                gCAAAGGGAAAAAGTGTTGGGAAAGAGTGTAAATAAATCAAAATATAACAAAAATCCAAATATTAAAAAAAAATTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGtt                                      >  2:1028038/1‑150 (MQ=255)
                                                                                                                gCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAATATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGtt                                      >  2:1491984/1‑150 (MQ=255)
                                                                                                                 cAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATAAAGCACAAATTTACAAAAATCCAATTTTTAAAAAAAAATTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGttt                                     >  2:241275/1‑149 (MQ=255)
                                                                                                                   aaGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGAt                                   >  2:941473/1‑150 (MQ=255)
                                                                                                                    atgtaaaaaGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATg                                  <  1:913885/146‑1 (MQ=255)
                                                                                                                    agtgAAAAATTTTGGGGAAAGAGTGTGAATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATg                                  <  2:105978/147‑1 (MQ=255)
                                                                                                                    agtgAAAAAGTGTGGGGAAAGAGTGTGCATGAATCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATg                                  <  1:907859/147‑1 (MQ=255)
                                                                                                                    aGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATg                                  <  2:376200/150‑1 (MQ=255)
                                                                                                                      ggAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAAATTACAAAAATGCAATTATGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGATTCAATGTTTATACATTTAGTTGATGCg                                >  2:79296/1‑150 (MQ=255)
                                                                                                                      ggAAAAAGTGTGGGGAAAGAGTGTACATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGATACTTTAGAGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATTCg                                >  2:83509/1‑150 (MQ=255)
                                                                                                                       gAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGt                               <  1:1215533/150‑1 (MQ=255)
                                                                                                                       gAAAAAGTGTAGGGAAAGAGTGTAAATAAATCAAAAATTAACAAAAATCCAATTATTAAAAAATACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGt                               >  2:536415/1‑150 (MQ=255)
                                                                                                                        aaaaaTTGTTGGGAAAGAGTGTGCATGAAGAACAAATTTACAAAAATCCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGtt                              <  1:310338/150‑1 (MQ=255)
                                                                                                                        aaaaaGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGtt                              <  2:719982/150‑1 (MQ=255)
                                                                                                                          aaaGTGTGTGGAAAGAGTGTGCATGAAGCACAAATTTACAAACATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCAAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTg                            <  1:1166251/150‑1 (MQ=255)
                                                                                                                           aaGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTATCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGa                           <  1:698367/150‑1 (MQ=255)
                                                                                                                             gtgtGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATg                         <  2:1002495/150‑1 (MQ=255)
                                                                                                                              tgtgGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATgt                        <  1:133515/150‑2 (MQ=255)
                                                                                                                                    gtaaatttgtgCTTCATGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACgg                  >  2:240896/8‑150 (MQ=255)
                                                                                                                                     aaaGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGa                 >  2:583333/1‑150 (MQ=255)
                                                                                                                                      aaGAGTGTGCATGAAGCACAAATTTACAAAACTGCAAGATTCATAAGAGAGTTTAGCGGTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGTCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGaa                <  2:137651/150‑1 (MQ=255)
                                                                                                                                         agTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCt             >  2:692221/1‑150 (MQ=255)
                                                                                                                                         agTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCt             <  1:482513/150‑1 (MQ=255)
                                                                                                                                            gtgCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGaa          <  1:1636313/150‑1 (MQ=255)
                                                                                                                                             tgCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAg         >  1:1415745/1‑150 (MQ=255)
                                                                                                                                             tgCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAg         <  1:239346/150‑1 (MQ=255)
                                                                                                                                              gCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCTTTGATGCGTTTGATGCACACGGAAGCTGAAGc        >  2:628462/1‑150 (MQ=255)
                                                                                                                                              gCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGc        <  1:297664/150‑1 (MQ=255)
                                                                                                                                                 tGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCa     <  1:573687/150‑1 (MQ=255)
                                                                                                                                                   aaGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCAGt   >  2:1103360/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCAGTA  >  NC_000913/3710486‑3710783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: