Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,716,508 A→T 5.4% intergenic (‑114/‑39) bisC ← / → yiaD biotin sulfoxide reductase/multicopy suppressor of bamB; outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,716,5080AT 5.4% 407.8 / 8.6 148intergenic (‑114/‑39)bisC/yiaDbiotin sulfoxide reductase/multicopy suppressor of bamB; outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (60/79);  new base T (3/5);  total (64/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

Only 100 of 148 total aligned reads displayed.

GAACGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTA  >  NC_000913/3716364‑3716655
                                                                                                                                                 |                                                                                                                                                   
gAACGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGt                                                                                                                                                >  1:1101780/1‑150 (MQ=255)
  aCGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCATGCGGCATAATCTCCCGCCACGGAACCCGTgg                                                                                                                                              <  1:1518758/150‑1 (MQ=255)
  aCGGAATATCGTGAGGATGAGTTGGCAAAACCGAAGACTCCTGACAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTgg                                                                                                                                              <  1:158291/150‑1 (MQ=255)
   cGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGc                                                                                                                                             <  1:463253/150‑1 (MQ=255)
      aaTATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGGTTCCGTGGcggg                                                                                                                                          <  1:1324481/150‑4 (MQ=255)
       atatCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACATATTGCCGAGTATTCACAGGATTAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGATCCCGTGGCAAAg                                                                                                                                         <  2:1108539/150‑1 (MQ=255)
       atatCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAg                                                                                                                                         <  1:1406894/150‑1 (MQ=255)
         atCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGGTTCCGTGGcgggaga                                                                                                                                       <  1:344542/150‑7 (MQ=255)
         atCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGGTTCCGTGGcgggaga                                                                                                                                       <  2:1146095/150‑7 (MQ=255)
          tCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAAt                                                                                                                                      <  2:1509890/150‑1 (MQ=255)
          tCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGATAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAAt                                                                                                                                      <  1:221728/150‑1 (MQ=255)
           cGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATa                                                                                                                                     <  1:997529/150‑1 (MQ=255)
              taGGATGAGGTTGCCAAACAGAAGACTCCTTAAATGTTATATGTATTTAGCAGTTAAATAATACTTACAACTTTCCGAGTATTCCCAGGACTAATCTTCATGTTCACGCGGCATACTCTCCCGCCACGGAACCCGTGGCAAAGAATaaaa                                                                                                                                  <  1:265850/149‑1 (MQ=255)
                ggATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAg                                                                                                                                <  2:122788/150‑1 (MQ=255)
                ggATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAg                                                                                                                                <  1:816211/150‑1 (MQ=255)
                    gAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGATTAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGtta                                                                                                                            >  1:1141891/1‑150 (MQ=255)
                     aGTTGTTCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACTAATTTCCGATTATTCGCATGAATATTCTTCATGTTCTCGCGGCATAATCTGCCGCCACGGAACCCGTGGCAAAGAATAAATAGGttat                                                                                                                           <  2:695590/150‑1 (MQ=255)
                     aGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCTTAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGttat                                                                                                                           >  2:501355/1‑150 (MQ=255)
                      gTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGttat                                                                                                                           <  1:1229195/150‑1 (MQ=255)
                      gTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGttatt                                                                                                                          >  1:873417/1‑150 (MQ=255)
                       ttGTCCAAACCGAAGACTCCTGTAAGGTTATATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCTTAATCTCCAGCCACGGAACCCGTGGCAAAGAATAAAAAGGttatta                                                                                                                         <  1:869904/150‑1 (MQ=255)
                          gCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAgg                                                                                                                      >  1:645605/1‑150 (MQ=255)
                            cAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATAATACTTACAAAATTCCGAGAATTCCCAGGAATAATCTTCATGTTCACGCGTCATAATCTCCCGCAACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGAt                                                                                                                    >  1:239617/1‑150 (MQ=255)
                              aaCCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTa                                                                                                                  <  1:359289/150‑1 (MQ=255)
                                ccGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGTCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAAc                                                                                                                <  1:1225045/150‑1 (MQ=255)
                                ccGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAAc                                                                                                                <  1:1281565/150‑1 (MQ=255)
                                ccGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAAc                                                                                                                >  1:665301/1‑150 (MQ=255)
                                  gAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACaa                                                                                                              <  1:1612926/150‑1 (MQ=255)
                                    aGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATg                                                                                                            <  1:1693345/150‑1 (MQ=255)
                                     gACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATga                                                                                                           <  2:803671/150‑1 (MQ=255)
                                      aCTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATgaa                                                                                                          >  1:812945/1‑150 (MQ=255)
                                       cTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATgaag                                                                                                         <  1:1683720/150‑1 (MQ=255)
                                       cTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATgaag                                                                                                         <  2:1087095/150‑1 (MQ=255)
                                        tCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATgaaga                                                                                                        <  2:371229/150‑1 (MQ=255)
                                         ccTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATgaagaa                                                                                                       >  1:505221/1‑150 (MQ=255)
                                           tGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAAc                                                                                                     <  1:922829/150‑1 (MQ=255)
                                              aaGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACgtg                                                                                                  >  1:1173952/1‑150 (MQ=255)
                                                 gTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGttt                                                                                               >  2:428936/1‑150 (MQ=255)
                                                 gTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGttt                                                                                               <  2:1720478/150‑1 (MQ=255)
                                                    aGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATc                                                                                            <  1:963078/150‑1 (MQ=255)
                                                     taTGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCt                                                                                           <  2:1367384/149‑1 (MQ=255)
                                                        gTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTAt                                                                                        >  2:931770/1‑150 (MQ=255)
                                                          aTTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTg                                                                                      >  2:1667350/1‑150 (MQ=255)
                                                            tGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTgcc                                                                                    <  2:361053/150‑1 (MQ=255)
                                                            tGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTgcc                                                                                    >  2:1267759/1‑150 (MQ=255)
                                                                   ttAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTag                                                                             <  1:329215/150‑1 (MQ=255)
                                                                     taaTATTACTTACAAAATTCCGAGTATACCCATGAATAATCTTAATGTTCTCGCGGCATAATCTACCGCCACGTAACCCGTGGAAAAGTATAAAAAGGTTATTAAGGATTAAAAATGATGAAACGTGTTTATGTTATTGCCGCCGTagtg                                                                           <  2:873485/149‑1 (MQ=255)
                                                                     aaaTATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtg                                                                           <  2:513496/150‑1 (MQ=255)
                                                                     aaaTATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtg                                                                           <  2:1102954/150‑1 (MQ=255)
                                                                       atatTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATATTCTCCCGCCACGGACCCCGTGGCAAAGAATAAAAAGGTTATTAAGGAATATCAATGAAGAAACGTGTTTATCTTATTGCCGGCGTagtgag                                                                         >  2:85291/1‑150 (MQ=255)
                                                                       atatTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtgag                                                                         <  2:645605/150‑1 (MQ=255)
                                                                       atatTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtgag                                                                         >  1:427079/1‑150 (MQ=255)
                                                                       atatTACTTACAAATTTCCGAGTATTCCAAGGAATAATCTTCATGTTCACGCGGCATAATCTCACGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtgag                                                                         <  1:288239/150‑1 (MQ=255)
                                                                        tataACTTACAAAATAACGACTATTCCAAGGAAAAATGTTAATGTTCACGCGGCATAAACTCCCGCCACGGAACGCGTTGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtgagt                                                                        <  2:251545/150‑1 (MQ=255)
                                                                        tatTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtgagt                                                                        <  2:37460/150‑1 (MQ=255)
                                                                         atTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTagtgagtg                                                                       >  1:2083/1‑150 (MQ=255)
                                                                          ttacttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTgg                                                                      <  1:428936/150‑1 (MQ=255)
                                                                          ttacttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTgg                                                                      <  1:893286/150‑1 (MQ=255)
                                                                          ttacttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTgg                                                                      >  1:1653245/1‑150 (MQ=255)
                                                                          tcacttacAAATTTCCGAGTATTCCCAGGAATAATCTGAATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTgg                                                                      <  2:273894/148‑1 (MQ=255)
                                                                           tacttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGgc                                                                     >  2:776629/1‑150 (MQ=255)
                                                                              ttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCAGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTAATCTTATTGCCGCCGTAGTGAGTGgcgat                                                                  >  1:220816/1‑148 (MQ=255)
                                                                                acAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGctct                                                                >  2:612459/1‑150 (MQ=255)
                                                                                 cAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTg                                                               >  2:553771/1‑150 (MQ=255)
                                                                                     aTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCg                                                            <  2:1653245/150‑1 (MQ=255)
                                                                                      tttCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCgg                                                           <  1:210021/150‑1 (MQ=255)
                                                                                      tttCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGAGTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCgg                                                           >  2:218896/1‑150 (MQ=255)
                                                                                      tttCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGGTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCgg                                                           <  1:301062/150‑1 (MQ=255)
                                                                                              tATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGc                                                   <  2:451142/150‑1 (MQ=255)
                                                                                              tATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGc                                                   <  1:931770/150‑1 (MQ=255)
                                                                                                ttCCCAGGAATAATCTTCATGTTCACGCGGCATACTCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTg                                                 <  1:322537/150‑1 (MQ=255)
                                                                                                 tCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATATAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGc                                                >  1:242700/1‑150 (MQ=255)
                                                                                                 tCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGc                                                >  2:1298167/1‑150 (MQ=255)
                                                                                                  cccAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGca                                               >  1:1552871/1‑150 (MQ=255)
                                                                                                   ccAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAGCGTGTTAATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGcac                                              >  1:340829/1‑150 (MQ=255)
                                                                                                          taatCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGt                                                                               >  1:571677/1‑110 (MQ=255)
                                                                                                          taatCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGt                                                                               <  2:571677/110‑1 (MQ=255)
                                                                                                                tCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGACTAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTac                                 <  2:1216124/150‑1 (MQ=255)
                                                                                                                tCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTTTTGCCGCCGTAGTGAGTGGCGATCTGGCGGTATCTGGCTGCACAACTAACCCTTac                                 >  2:138320/1‑150 (MQ=255)
                                                                                                                  aTGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTacac                               >  1:710503/1‑150 (MQ=255)
                                                                                                                  aTGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTacac                               >  2:29990/1‑150 (MQ=255)
                                                                                                                             gCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAAcgcg                    >  1:1180061/1‑150 (MQ=255)
                                                                                                                              cATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGa                   <  2:710503/150‑1 (MQ=255)
                                                                                                                                tAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAg                 >  2:107474/1‑150 (MQ=255)
                                                                                                                                 aaTCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGc                <  2:323659/150‑1 (MQ=255)
                                                                                                                                  attctttgCCACGGGTTCCGTGGCGAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCa               >  1:1497221/8‑150 (MQ=255)
                                                                                                                                   tctcCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAg              >  2:1518523/1‑150 (MQ=255)
                                                                                                                                   tctcCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAg              >  1:601612/1‑150 (MQ=255)
                                                                                                                                   tctcCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAg              <  1:581562/150‑1 (MQ=255)
                                                                                                                                    ctcCCGCCACGGAACCCGTGGCAAATAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTAATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAgg             <  2:340829/150‑1 (MQ=255)
                                                                                                                                    ctcCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAgg             >  1:240330/1‑150 (MQ=255)
                                                                                                                                       ttgCCACGGGTTCCGTGGCGAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTaa          >  2:1157626/3‑150 (MQ=255)
                                                                                                                                        cGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTaaa         >  1:705098/1‑150 (MQ=255)
                                                                                                                                         gCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAAt        >  1:1629936/1‑150 (MQ=255)
                                                                                                                                           cACGGAACCCGTGGCAAAGAATAAAAACGTTATTAAGGATTAACAGTGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCt      <  1:140691/150‑1 (MQ=255)
                                                                                                                                             cGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGc    <  1:612459/150‑1 (MQ=255)
                                                                                                                                              ggAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCt   <  2:818674/150‑1 (MQ=255)
                                                                                                                                              ggAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGAAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCt   >  1:114603/1‑150 (MQ=255)
                                                                                                                                              ggAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGAAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCt   >  1:1547922/1‑150 (MQ=255)
                                                                                                                                              ggAACCAGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCt   <  2:250332/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GAACGGAATATCGTGAGGATGAGTTGGCCAAACCGAAGACTCCTGAAAGGTTAGATGTATTGAGCAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTA  >  NC_000913/3716364‑3716655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: