BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,720,797	A→C	5.4%	R40R (AGG→CGG) ‡	insJ →	IS150 transposase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,720,797	0	A	C	5.4%	394.7 / 9.5	130	R40R (AGG→CGG) ‡	insJ	IS150 transposase A
Reads supporting (aligned to +/- strand): ref base A (61/62); new base C (2/5); total (63/67)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 130 total aligned reads displayed.

GTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCAC  >  NC_000913/3720650‑3720943

 ttgGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGa                                                                                                                                                 <  1:895236/150‑1 (MQ=255)

  tgGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGAc                                                                                                                                                >  2:1473811/1‑150 (MQ=255)

  tgGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGAc                                                                                                                                                >  2:410665/1‑150 (MQ=255)

   ggACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACa                                                                                                                                               <  2:206163/150‑1 (MQ=255)

    gACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACAt                                                                                                                                              >  2:165238/1‑150 (MQ=255)

     aCGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATg                                                                                                                                             <  1:243169/150‑1 (MQ=255)

         tGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGtt                                                                                                                                         >  1:1671932/1‑150 (MQ=255)

          gAAATGGAATATCCCCTAATATGTCAAAGCAAAAATACCCTTTTCAAAAGCGACTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCATGTCCTGACCTGGGttc                                                                                                                                        <  2:1584119/150‑2 (MQ=255)

          gAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTg                                                                                                                                        <  2:687377/150‑1 (MQ=255)

           aaaTGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCAAAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGc                                                                                                                                       >  1:1308863/1‑150 (MQ=255)

            aaTGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCATGTCCTGACCTGGGttcgg                                                                                                                                      <  1:1174336/150‑4 (MQ=255)

            aaTGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGcc                                                                                                                                      >  2:720602/1‑150 (MQ=255)

             aTGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCATGTCCTGACCTGGGttcggg                                                                                                                                     <  2:903522/150‑5 (MQ=255)

             aTGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGccc                                                                                                                                     <  2:612304/150‑1 (MQ=255)

                gAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCctct                                                                                                                                  <  2:1328001/150‑1 (MQ=255)

                   aaGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATg                                                                                                                               <  1:1551325/149‑1 (MQ=255)

                    agacccTAAAATGTCAAAGCCAAAATACCCTTATGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTCATGATGGTTACAGCATCATCTAGGCACGTTTTGGTGTCCCCCGAACCCAGGACAGGACAAGTGTTGCCCTCTATGa                                                                                                                              >  2:826750/4‑150 (MQ=255)

                     gCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTAACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCAAGGTCAGGACATGGGTTGCCCTCTATGaa                                                                                                                             >  2:146470/1‑150 (MQ=255)

                      ccccTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGaaa                                                                                                                            >  2:1270194/1‑150 (MQ=255)

                      ccccTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGGGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGGGGCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGaaa                                                                                                                            <  2:441895/150‑1 (MQ=255)

                       cccTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGaaaa                                                                                                                           >  2:1374039/1‑150 (MQ=255)

                         cTAATATGTCAAAGCCAAAAAACCCTTTTGAAAAGCGCCTTGAAGCCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGTTTGCCCTCTATGAAAAAc                                                                                                                         <  1:295903/150‑1 (MQ=255)

                             tatGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATgg                                                                                                                     <  1:410665/150‑1 (MQ=255)

                             tatGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATgg                                                                                                                     <  1:1473811/150‑1 (MQ=255)

                             tatGACAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATgg                                                                                                                     <  1:356874/150‑1 (MQ=255)

                              atGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGga                                                                                                                    <  1:1451786/150‑1 (MQ=255)

                              atGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGga                                                                                                                    >  2:1329455/1‑150 (MQ=255)

                               tGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACa                                                                                                                                               <  1:1099355/122‑1 (MQ=255)

                               tGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACa                                                                                                                                               >  2:1099355/1‑122 (MQ=255)

                               tGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCGCTCTATGAAAAACATGgag                                                                                                                   <  2:1025832/150‑1 (MQ=255)

                               tGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGGCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGgag                                                                                                                   <  2:1289865/150‑1 (MQ=255)

                                gTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAAGTCAGGACATGGGTTGCCCTCTATGAAAAACATGgaga                                                                                                                  >  2:307020/1‑150 (MQ=255)

                                    aaGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTACAAAACTGATCATGGTTTCAGGAACATCTCGGCACGTTTTGGTTACCGCCGAACCCAGGTCAGGACATGGTTTGCCCGCTATTAAAAACATGTAGaaaaa                                                                                                              >  2:1641939/1‑150 (MQ=255)

                                      gCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAgg                                                                                                            <  2:1472813/150‑1 (MQ=255)

                                      gCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAgg                                                                                                            <  1:1104560/150‑1 (MQ=255)

                                       ccAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGt                                                                                                           >  2:412040/1‑150 (MQ=255)

                                       ccAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGATCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGt                                                                                                           <  1:1628271/150‑1 (MQ=255)

                                       ccAAAATAACCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTAACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGGCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGt                                                                                                           <  2:1568021/150‑1 (MQ=255)

                                           aaTACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACCACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTaa                                                                                                       >  1:1561712/1‑150 (MQ=255)

                                             tACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAAtt                                                                                                     >  2:622667/1‑150 (MQ=255)

                                             tACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAAtt                                                                                                     <  1:1632025/150‑1 (MQ=255)

                                              aCCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTc                                                                                                    <  1:1337553/150‑1 (MQ=255)

                                              aCCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTc                                                                                                    <  1:722864/150‑1 (MQ=255)

                                                 cTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCa                                                                                                 >  2:933420/1‑150 (MQ=255)

                                                    ttGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGAGCATCTCGGCCCGTTTTGGTGTCCCCCGACCCCAGGTCAGGACATGGGTTGCCGTCTATGAAAACCATGGAGAAAAAGGTTTAATTCCCAAAc                                                                                              >  1:1646074/1‑150 (MQ=255)

                                                     tGAAAAGCGCCTTGAAGTCGTTAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAAcc                                                                                             <  1:1674565/150‑1 (MQ=255)

                                                     tGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAAcc                                                                                             <  1:149753/150‑1 (MQ=255)

                                                               cTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGCCATGGGTTGCCCTCTATGAAAAACATGGAGAAAAACGTTTAATTCCCAAACCTAAAGGAGtt                                                                                   >  2:123116/1‑150 (MQ=255)

                                                                  gAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAAACTAAAGGCGTTAGt                                                                                >  2:1525367/1‑150 (MQ=255)

                                                                   aaGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTg                                                                               <  1:1656231/150‑1 (MQ=255)

                                                                    aGTCGTGAATCACTACTTCTCAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGc                                                                              >  1:6222/1‑150 (MQ=255)

                                                                    aGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGc                                                                              >  2:551547/1‑150 (MQ=255)

                                                                    aGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGAGTTAGTGc                                                                              >  2:829546/1‑150 (MQ=255)

                                                                     gTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGAACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTACCAAACCTAAAGGCGTTAGTGCt                                                                             >  1:1306540/1‑150 (MQ=255)

                                                                     gTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATAATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTACCAAACCTAAAGGCGTTAGTGCt                                                                             >  2:591576/1‑150 (MQ=255)

                                                                        gTGAATCACTACTTCACAACTGATGATGGTTTCAGGATCATCTCGGCACGTTTTGGTGTCACCCGAACACAGGTCAGGACATGGGTTGCCATCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGAt                                                                          <  2:451039/150‑1 (MQ=255)

                                                                        gTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGAt                                                                          <  2:1671932/150‑1 (MQ=255)

                                                                          gAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATcc                                                                        >  1:995374/1‑150 (MQ=255)

                                                                            aTCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCag                                                                      <  2:1395770/150‑1 (MQ=255)

                                                                            aTCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCag                                                                      <  2:736253/150‑1 (MQ=255)

                                                                            aTCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCag                                                                      >  1:686710/1‑150 (MQ=255)

                                                                             tCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGCACGCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATACCCAAACCTAAAGGCGTTAGTGCTGATCCata                                                                     >  2:316464/1‑148 (MQ=255)

                                                                              cACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCagag                                                                    <  2:271424/150‑1 (MQ=255)

                                                                               actactTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGt                                                                   <  2:12121/150‑1 (MQ=255)

                                                                                   ctTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCg                                                               <  2:1020235/150‑1 (MQ=255)

                                                                                        caACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTa                                                          <  2:228611/150‑1 (MQ=255)

                                                                                        aaaCTGATGATGGTTACAGTATTTTCTCGTCACGTTTTTGTGTGCCCAGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAACCATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTa                                                          <  1:260765/149‑1 (MQ=255)

                                                                                         aaCTGATGATGGTTACCGGATCATCTCGCCACGTTTTGGTGTCCCCAGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCATAGTTGCGTATTaa                                                         >  1:286790/1‑150 (MQ=255)

                                                                                            tgatgaTGGTTACAGGAGCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGt                                                      <  2:821353/150‑1 (MQ=255)

                                                                                             gatgatGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCAAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATACCCAAACCTAAAGGCGTTAGTGCTAATCAAGAGTTGTGTATTAAGGTc                                                     >  1:716799/1‑150 (MQ=255)

                                                                                              atgatgGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGGCg                                                    <  1:640062/150‑1 (MQ=255)

                                                                                               tgatgGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGt                                                   <  1:1374039/150‑1 (MQ=255)

                                                                                                gatgGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTg                                                  >  2:1579749/1‑150 (MQ=255)

                                                                                                    gTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGGGAAAg                                              <  1:1285077/150‑1 (MQ=255)

                                                                                                      tACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCt                                            >  1:903825/1‑150 (MQ=255)

                                                                                                       aCAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCtg                                           <  1:969584/150‑1 (MQ=255)

                                                                                                             tcatcTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCg                                     >  2:561188/1‑150 (MQ=255)

                                                                                                             tcatcTCGGCACGTTTTGGCGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTCTGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCg                                     <  2:1247144/150‑1 (MQ=255)

                                                                                                                  tcGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGagcag                                <  1:243194/150‑1 (MQ=255)

                                                                                                                   cGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGagcagc                               >  2:1215289/1‑150 (MQ=255)

                                                                                                                     gCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGcac                             <  1:429713/150‑1 (MQ=255)

                                                                                                                        cGTTTTGGTGTCCCCCGAACCCAAGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATg                          <  1:307020/150‑1 (MQ=255)

                                                                                                                         gTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGt                         <  2:177450/150‑1 (MQ=255)

                                                                                                                          ttttGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTc                        >  2:883887/1‑150 (MQ=255)

                                                                                                                           tttGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTcc                       >  1:1464607/1‑150 (MQ=255)

                                                                                                                             tGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAGGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCt                     <  1:449696/150‑1 (MQ=255)

                                                                                                                             tGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCt                     <  1:412040/150‑1 (MQ=255)

                                                                                                                              ggTGTCCCCCGAACCCAGGTCACGACATGGGATCCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCAAAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCtt                    >  2:1580054/1‑150 (MQ=255)

                                                                                                                               gtgtCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTg                                                                               <  1:1563321/90‑1 (MQ=255)

                                                                                                                               gtgtCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTg                                                                               >  2:1563321/1‑90 (MQ=255)

                                                                                                                                tgtCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTaa                  >  2:4434/1‑150 (MQ=255)

                                                                                                                                   cccccGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCa               >  1:541602/1‑150 (MQ=255)

                                                                                                                                    ccccGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAg              >  1:1431774/1‑150 (MQ=255)

                                                                                                                                       gcaaCCCATGTCCTGACCTGGGTTGCCCTCTATGAAAAACATGGAGAAAAATGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTACATTAATCAGgct           >  1:1722934/3‑150 (MQ=255)

                                                                                                                                       gcaaCCCATGTCCTGACCTGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGgct           >  1:493448/3‑150 (MQ=255)

                                                                                                                                          aCCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGgctgct        <  1:720602/150‑1 (MQ=255)

                                                                                                                                          aCCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGgctgct        <  1:622667/150‑1 (MQ=255)

                                                                                                                                             cAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGgctgctgct     <  2:686710/150‑1 (MQ=255)

                                                                                                                                               ggTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCa   >  1:1490465/1‑150 (MQ=255)

                                                                                                                                               ggTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCa   >  2:206466/1‑150 (MQ=255)

GTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATGTCCCTTAATCAGGCTGCTGCTCAC  >  NC_000913/3720650‑3720943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`