Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,722,300 G→T 5.8% intergenic (+195/+28) sokA → / ← glyS pseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,722,3000GT 5.8% 520.3 / 15.6 172intergenic (+195/+28)sokA/glySpseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (73/89);  new base T (5/5);  total (78/94)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01

Only 100 of 172 total aligned reads displayed.

CCCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAG  >  NC_000913/3722152‑3722445
                                                                                                                                                    |                                                                                                                                                 
cccGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGa                                                                                                                                                  <  2:526732/150‑1 (MQ=255)
  cGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATg                                                                                                                                                >  1:853801/1‑150 (MQ=255)
        tATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTTATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGc                                                                                                                                          <  2:364040/150‑1 (MQ=255)
           tCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAt                                                                                                                                       >  1:1194797/1‑150 (MQ=255)
             tAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCTtttt                                                                                                                                     <  2:846269/150‑1 (MQ=255)
             tAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAttt                                                                                                                                     <  1:1646646/150‑1 (MQ=255)
             tAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAttt                                                                                                                                     <  2:740059/150‑1 (MQ=255)
              aGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTta                                                                                                                                    <  2:558716/150‑1 (MQ=255)
                  taataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTTTTCAGCAGAAGATTAGTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCATGACAGATGGATGGCTATTtaataa                                                                                                                                >  2:316571/1‑150 (MQ=255)
                  taataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCTttttttatc                                                                                                                                <  2:1610737/150‑6 (MQ=255)
                  taataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaataa                                                                                                                                >  2:1430970/1‑150 (MQ=255)
                  taataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGATGTATTATTTAGAGATAAAAAAAGCCTGCCAGATGGCAGTCTATTtaataa                                                                                                                                >  1:1348937/1‑150 (MQ=255)
                   aataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCTttttttatcg                                                                                                                               <  2:188190/150‑7 (MQ=255)
                   aataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGACAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAAc                                                                                                                               <  1:1560650/150‑1 (MQ=255)
                    ataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACg                                                                                                                              <  1:1034399/150‑1 (MQ=255)
                     taaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACgg                                                                                                                             <  2:413585/150‑1 (MQ=255)
                      aaGTTTGTTTCTTCTACTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGc                                                                                                                            <  2:331278/150‑1 (MQ=255)
                        gtttgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGt                                                                                                                          >  1:576047/1‑150 (MQ=255)
                         tttgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGtt                                                                                                                         <  2:1146671/150‑1 (MQ=255)
                           tgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttat                                                                                                                       <  2:131618/150‑1 (MQ=255)
                             tttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttatta                                                                                                                     >  2:1041033/1‑150 (MQ=255)
                              ttcttcTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTtattat                                                                                                                    <  2:717451/150‑1 (MQ=255)
                                cttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTg                                                                                                                  <  1:997703/150‑1 (MQ=255)
                                cttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTg                                                                                                                  <  1:1317783/150‑1 (MQ=255)
                                cttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTg                                                                                                                  <  2:940992/150‑1 (MQ=255)
                                 ttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGc                                                                                                                 <  1:32712/150‑1 (MQ=255)
                                  tctcCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCa                                                                                                                >  1:673841/1‑150 (MQ=255)
                                     ccTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                             >  1:551321/1‑150 (MQ=255)
                                       ttGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGc                                                                                                           >  2:1532257/1‑150 (MQ=255)
                                        tGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCg                                                                                                          >  2:425368/1‑150 (MQ=255)
                                         gATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGa                                                                                                         >  2:1647184/1‑150 (MQ=255)
                                             taATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTTTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAata                                                                                                     <  1:824572/150‑1 (MQ=255)
                                             taATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAata                                                                                                     <  1:68595/150‑1 (MQ=255)
                                              aaTCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAatat                                                                                                    >  1:1450497/1‑150 (MQ=255)
                                                tCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGATGCTGTATAATTTAGGGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATcc                                                                                                  <  1:1124053/150‑1 (MQ=255)
                                                tCCTAAAAAAATCTCATAAAATTAAAATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATcc                                                                                                  <  1:1098671/150‑1 (MQ=255)
                                                 ccTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCg                                                                                                 <  1:768684/150‑1 (MQ=255)
                                                  cTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGc                                                                                                >  1:616552/1‑150 (MQ=255)
                                                  cTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGc                                                                                                >  1:166734/1‑150 (MQ=255)
                                                   tAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCa                                                                                               <  2:659597/150‑1 (MQ=255)
                                                    aaaaaaaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCaa                                                                                              >  2:899099/1‑150 (MQ=255)
                                                     aaaaaaTCTCATAAAATTAATATATTAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATAATTTAGCGATAAAAAAAGCCTGACAGATGGCATGCTATATAAAAACGGCGATATAATTGCAACAGAGAAAAATCCTCAAc                                                                                             >  2:918395/1‑150 (MQ=255)
                                                               aTAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAAc                                                                                   <  2:757283/150‑1 (MQ=255)
                                                                 aaaaTTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAg                                                                                 <  1:1021948/150‑1 (MQ=255)
                                                                       aaTATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTcgcg                                                                           <  1:879724/150‑1 (MQ=255)
                                                                           tatGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGt                                                                       >  2:1101634/1‑150 (MQ=255)
                                                                            atGATATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGtt                                                                      <  2:853801/150‑1 (MQ=255)
                                                                            atGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGtt                                                                      <  1:424327/150‑1 (MQ=255)
                                                                            atGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGtt                                                                      <  1:1599055/150‑1 (MQ=255)
                                                                             tGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGttt                                                                     >  1:951173/1‑150 (MQ=255)
                                                                               agaTAATCTTTATTCAGCAGAAGATTATTCAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtct                                                                   >  1:1574530/1‑150 (MQ=255)
                                                                                gaTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtctc                                                                  >  1:844374/1‑150 (MQ=255)
                                                                                 aTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTcc                                                                 <  2:1689507/150‑1 (MQ=255)
                                                                                  tAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTTCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTACa                                                                >  1:317770/1‑150 (MQ=255)
                                                                                  tAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCa                                                                >  1:1613458/1‑150 (MQ=255)
                                                                                    aTCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTTTTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGc                                                              >  1:956483/1‑150 (MQ=255)
                                                                                     tCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGCCGTTATTATTGCAACAGCGAAATAACCGCAACGCGCAGGAACAGTTCGCGCACTTTCACCAGCa                                                             >  2:320406/1‑150 (MQ=255)
                                                                                        ttATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATgg                                                          <  1:1211846/150‑1 (MQ=255)
                                                                                          aTTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTc                                                        <  1:673555/150‑1 (MQ=255)
                                                                                          aTTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTc                                                        <  1:1422609/150‑1 (MQ=255)
                                                                                           ttCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCa                                                       <  2:184650/150‑1 (MQ=255)
                                                                                             cagcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGa                                                     <  2:166734/150‑1 (MQ=255)
                                                                                                 agaagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGt                                                 <  2:1194797/150‑1 (MQ=255)
                                                                                                 agaagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTACTCCAGCATGGTCAGACGGt                                                 >  2:27712/1‑150 (MQ=255)
                                                                                                     gaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGAt                                             <  2:673841/150‑1 (MQ=255)
                                                                                                     gaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGAt                                             <  2:576047/150‑1 (MQ=255)
                                                                                                     gaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGAt                                             <  2:1197655/150‑1 (MQ=255)
                                                                                                     gaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAAAAACGGCGTTATTATTGAAACAGCGAAATATCCGAAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGACAGACGGTTgct                                             >  1:326552/1‑148 (MQ=255)
                                                                                                         attaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGc                                         >  1:416660/1‑150 (MQ=255)
                                                                                                         attaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGAAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGCCGGTTGATACGc                                         >  1:177616/1‑150 (MQ=255)
                                                                                                           taAAGGTTGCTGAATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCaa                                       >  2:985341/1‑150 (MQ=255)
                                                                                                            aaaGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAAt                                      >  2:583408/1‑150 (MQ=255)
                                                                                                               ggTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCt                                   >  1:1456140/1‑150 (MQ=255)
                                                                                                                   gCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGt                               <  1:393758/150‑1 (MQ=255)
                                                                                                                    cTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtc                              <  2:195716/150‑1 (MQ=255)
                                                                                                                    cTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtc                              <  2:810317/150‑1 (MQ=255)
                                                                                                                        attattTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcatca                          <  1:967975/150‑1 (MQ=255)
                                                                                                                         ttattTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCaa                         <  1:1147977/150‑1 (MQ=255)
                                                                                                                          tattTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAAc                        >  2:627525/1‑150 (MQ=255)
                                                                                                                           attTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAAcc                       <  2:233933/150‑1 (MQ=255)
                                                                                                                           attTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAAcc                       >  2:730210/1‑150 (MQ=255)
                                                                                                                              tAGCGATAAAAAAAGCCTTCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATg                    <  2:408484/150‑1 (MQ=255)
                                                                                                                              tAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATg                    <  1:583408/150‑1 (MQ=255)
                                                                                                                               aGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGa                   <  1:873673/150‑1 (MQ=255)
                                                                                                                                 gttattaaataGCCTGCCATCTGGCAGGCTTTTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGAcc                 >  2:518127/7‑150 (MQ=255)
                                                                                                                                 gttattaaataGCCTGCCATCTGGCAGGCTTTTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGAcc                 >  2:88031/7‑150 (MQ=255)
                                                                                                                                 cGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACAATGAcc                 >  2:1417682/1‑150 (MQ=255)
                                                                                                                                 cGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGGTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGATCAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGAcc                 <  2:1284971/150‑1 (MQ=255)
                                                                                                                                  gATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCa                >  1:979399/1‑150 (MQ=255)
                                                                                                                                    tAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATc              >  1:1577134/1‑150 (MQ=255)
                                                                                                                                       aaataGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTTTTATTGCAACAGCGCAATATCCGCAACGCGCAGGAACAGATCGCGCAGTTACTCCAGCATGGTCAGACGGTTGCTACGCAATTCTTTGTCATCCACCATGACCATCACt           >  1:2559/1‑150 (MQ=255)
                                                                                                                                       aaataGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACt           >  2:562666/1‑150 (MQ=255)
                                                                                                                                       aaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACt           <  1:20981/150‑1 (MQ=255)
                                                                                                                                        aaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACtt          >  1:481212/1‑150 (MQ=255)
                                                                                                                                           aGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTAt       <  2:649856/150‑1 (MQ=255)
                                                                                                                                           aGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTAt       <  2:1167347/150‑1 (MQ=255)
                                                                                                                                            gCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATc      <  2:1467894/150‑1 (MQ=255)
                                                                                                                                            gCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTAGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATc      <  1:221845/150‑1 (MQ=255)
                                                                                                                                             ccTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCg     <  1:1532257/150‑1 (MQ=255)
                                                                                                                                             ccTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCg     <  2:1456140/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAG  >  NC_000913/3722152‑3722445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: