Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,732,136 A→C 5.6% intergenic (+13/‑65) xylF → / → xylG D‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,732,1360AC 5.6% 373.8 / 11.0 141intergenic (+13/‑65)xylF/xylGD‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base A (71/62);  new base C (4/4);  total (75/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

Only 100 of 141 total aligned reads displayed.

ACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAA  >  NC_000913/3731988‑3732283
                                                                                                                                                    |                                                                                                                                                   
 ccAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCgc                                                                                                                                                   >  1:1362670/1‑150 (MQ=255)
       gCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAg                                                                                                                                             >  1:1344066/1‑150 (MQ=255)
         aGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTATGCGTTACGCCCCAGCGCGGAGCg                                                                                                                                           >  1:1098380/1‑150 (MQ=255)
           aTACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGAGACCGATCGATGTGAATATAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCggg                                                                                                                                         <  1:199986/150‑1 (MQ=255)
           aTACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCggg                                                                                                                                         >  2:450334/1‑150 (MQ=255)
               cacaCTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCg                                                                                                                                     >  1:431520/1‑150 (MQ=255)
               aacacTTAATAATGGCCTCAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATTTTAATAAAAACAACATCAAAGATACGGTAATTAAAGACTGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCg                                                                                                                                     <  2:231334/149‑1 (MQ=255)
                acacTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAATAGAGCGAGCTGTAAGCGATACGCCCCACCGCGGAGCGGGGGCGt                                                                                                                                    >  1:411883/1‑150 (MQ=255)
                  acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTgt                                                                                                                                  >  1:631055/1‑149 (MQ=255)
                   cTGAATAATGGCCTGAACGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTCAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaac                                                                                                                                 <  1:489396/150‑4 (MQ=255)
                   cTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaac                                                                                                                                 <  2:1362670/150‑4 (MQ=255)
                   atGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAt                                                                                                                                 <  1:622376/149‑1 (MQ=255)
                     gAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAttt                                                                                                                               >  2:728160/1‑150 (MQ=255)
                     caataatGGCCTGACAGATGTCCCCACCCGCCTCCTGACACCGCTCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAACGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAttt                                                                                                                               <  2:518342/149‑1 (MQ=255)
                         aatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGACTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctct                                                                                                                           <  1:701234/150‑1 (MQ=255)
                           tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTcc                                                                                                                         <  2:1399985/150‑1 (MQ=255)
                           tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTcc                                                                                                                         <  2:878219/150‑1 (MQ=255)
                            ggCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGCTTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCa                                                                                                                        >  1:1058659/1‑150 (MQ=255)
                            ggCCTGAAAGATGTCCCCTCCCCCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGCGCGAGCTGTAATCGTAACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCa                                                                                                                        >  2:1651528/1‑150 (MQ=255)
                             gCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCAt                                                                                                                       >  2:740555/1‑150 (MQ=255)
                              ccTGAAAGATGTCCCCTCCCGCATCCTGACACCGATCGATGTGAATAAAAAAAACATCAAAGATACGGTAATTAAAGACGCATTCCACAAAGAGAGCGAGCTTTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGGGATTTCTCTCCCTg                                                                                                                      >  2:1636996/1‑150 (MQ=255)
                               cTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGc                                                                                                                     <  2:709460/150‑1 (MQ=255)
                                tGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGcc                                                                                                                    >  2:1703749/1‑150 (MQ=255)
                                 gAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGCTTCCACAAAGAGAGCGAGCTCTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGcag                                                                                                                   >  1:279683/1‑148 (MQ=255)
                                 gAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcg                                                                                                                   <  2:1280852/150‑1 (MQ=255)
                                  aaaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgc                                                                                                                  <  2:1028099/150‑1 (MQ=255)
                                   aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGCGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                 >  2:1369206/1‑150 (MQ=255)
                                       tGTCCCCTCCCGCCTCCTGACACCGATCGATTTGAATAAAAAGAACATCAACGATACGGTAATTAAAGCCGGATTCCAGAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaa                                                                                                             <  1:1575173/150‑1 (MQ=255)
                                        gTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaat                                                                                                            <  2:1320309/150‑1 (MQ=255)
                                        gTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaat                                                                                                            >  1:1127232/1‑150 (MQ=255)
                                         tCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTGCTCTCCATGCCGCGtgaatg                                                                                                           >  1:301922/1‑150 (MQ=255)
                                         tCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATAAAAGACGGAATCCACAAAGAGAGCGAGCTGAAAGCGTTACGCCCCAGCGCGCAGCGGGGCCGTGATTTCTCTCAAAGCCGCGtgaatg                                                                                                           >  2:544604/1‑150 (MQ=255)
                                          ccccTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatga                                                                                                          >  2:1006906/1‑150 (MQ=255)
                                             cTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAAtt                                                                                                       <  1:358415/150‑1 (MQ=255)
                                                 cGCCCCCTGATACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCACGTGAATGAATTGGCt                                                                                                   <  2:45631/150‑1 (MQ=255)
                                                    ctcctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCAATACAGCGTGAATGAATTGGCTTAg                                                                                                >  1:634651/1‑150 (MQ=255)
                                                        tGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAGAGAGAGCGCGCTGTAAGCGTTACGCCCCAGAGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTgg                                                                                            >  2:174399/1‑150 (MQ=255)
                                                        tGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTgg                                                                                            <  1:1724838/150‑1 (MQ=255)
                                                        cgACACCGATCGATGTGAATAACAACAACCTCAAAGATACGGTAATTAAAGACGGATACCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTgg                                                                                            <  2:323341/149‑1 (MQ=255)
                                                            acCGATCGATGTGAATAAAAACAACATAAAAGATACGGTAATTAACGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGTGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTc                                                                                        <  2:1458556/150‑1 (MQ=255)
                                                             ccGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCg                                                                                       >  2:365346/1‑150 (MQ=255)
                                                               gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                     <  2:1384609/150‑1 (MQ=255)
                                                               gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                     >  1:545253/1‑150 (MQ=255)
                                                                   gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCGTAGGTGGAGTCGTTATGc                                                                                 >  1:1564242/1‑150 (MQ=255)
                                                                    ttgtgAATAAACACCACATCAACGATACGGTAATTACAGACGGCTTCCACACCGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGcc                                                                                <  1:1471170/149‑1 (MQ=255)
                                                                    atGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGcc                                                                                <  1:379303/150‑1 (MQ=255)
                                                                    atGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGGAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGcc                                                                                <  1:1114521/150‑1 (MQ=255)
                                                                     tgtgAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCt                                                                               >  1:1654271/1‑150 (MQ=255)
                                                                        gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGTCGCGTGAATGAATTGGGTTAGGTGGAGTCGTTATGCCtttt                                                                            >  2:482793/1‑148 (MQ=255)
                                                                        gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTAt                                                                            >  1:476753/1‑150 (MQ=255)
                                                                         aaTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATc                                                                           >  1:797442/1‑150 (MQ=255)
                                                                          aTAAAAACAACATCAAAGATACGGTATTTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                          >  1:1473279/1‑150 (MQ=255)
                                                                          aTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                          >  2:1246329/1‑150 (MQ=255)
                                                                            aaaaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTAc                                                                        >  2:1011648/1‑150 (MQ=255)
                                                                             aaaaCATCATCAAAGTTACGGTAATTAAAGTCGGTTTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                       <  2:411883/150‑1 (MQ=255)
                                                                               aacaacATCAAAGATACGGTAATTACAGACGGCTTCCCCAAAGAGAGCGTGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTg                                                                     <  2:1333342/150‑1 (MQ=255)
                                                                                     aTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATga                                                               <  2:750352/150‑1 (MQ=255)
                                                                                     aTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTAATTTAAATga                                                               >  2:1422406/1‑150 (MQ=255)
                                                                                      tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaa                                                              >  1:1389967/1‑150 (MQ=255)
                                                                                       cAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGATATgaag                                                             >  2:265326/1‑150 (MQ=255)
                                                                                       cAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaag                                                             >  1:248686/1‑150 (MQ=255)
                                                                                        aaaGATACGGTAATTAAATACGGATTCCACAAAGAGATCGAGCTGTAAGCGTTACGCCCCAGCGCGTAGCGGGTGCGTGATTTCTCTACATTCCTCTTTAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaaga                                                            <  1:1184833/150‑1 (MQ=255)
                                                                                        aaaGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaaga                                                            <  1:1703749/150‑1 (MQ=255)
                                                                                          aGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAAc                                                          <  1:1559207/150‑1 (MQ=255)
                                                                                           gATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACa                                                         <  2:475444/150‑1 (MQ=255)
                                                                                             tACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACAtt                                                       >  2:1498799/1‑150 (MQ=255)
                                                                                                 gTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCa                                                   >  1:1021658/1‑150 (MQ=255)
                                                                                                   aaTTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGATTTGGCTTAGGTGGAGTCGTTATGCCTGATCTACTTGAAATGAAGACCATTACCaaa                                                 >  2:1068289/1‑150 (MQ=255)
                                                                                                    aTTAAAGACGTATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCaaaa                                                <  2:937529/150‑1 (MQ=255)
                                                                                                    aTTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCaaaa                                                >  1:350026/1‑150 (MQ=255)
                                                                                                     ttAATGACGGAGTCTACAACGACAGCGCGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGTCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGGCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAAc                                               <  2:281192/150‑1 (MQ=255)
                                                                                                     ttAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATTAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAAc                                               <  2:138992/150‑1 (MQ=255)
                                                                                                       aaaGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACTCCCCAGCGCGGAGCGGGGGCGTGAGTTCTCTCAATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATTGCTTATCTACTTGAAATGAATAACATTACAAAAACCt                                             >  2:431871/1‑150 (MQ=255)
                                                                                                        aaGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCtt                                            >  2:1351892/1‑150 (MQ=255)
                                                                                                          gACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGTAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCg                                          <  1:1051430/150‑1 (MQ=255)
                                                                                                              gATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAg                                      <  1:700093/150‑1 (MQ=255)
                                                                                                              gATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTCGCTTAGGTGGAGTCGTTATGCCTTATCTCCTTGAAATGAAGCACATTACCAACACCTTCGGCAg                                      >  1:55267/1‑150 (MQ=255)
                                                                                                               aTTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAgt                                     >  1:1053437/1‑150 (MQ=255)
                                                                                                                 tCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAgtgt                                   <  1:686531/150‑1 (MQ=255)
                                                                                                                      aaaGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAgg                              <  1:1611458/150‑1 (MQ=255)
                                                                                                                       aaGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGc                             >  2:1426409/1‑150 (MQ=255)
                                                                                                                           gagcgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGCtt                         >  1:250842/1‑150 (MQ=255)
                                                                                                                            agcgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTg                        >  2:507401/1‑150 (MQ=255)
                                                                                                                                agcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATaa                    >  1:1049719/1‑150 (MQ=255)
                                                                                                                                 gcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAAc                   <  1:740555/150‑1 (MQ=255)
                                                                                                                                 gcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAAc                   >  2:1330502/1‑150 (MQ=255)
                                                                                                                                  cTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACg                  >  2:387852/1‑150 (MQ=255)
                                                                                                                                    gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGATTGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGta                >  2:1191474/1‑149 (MQ=255)
                                                                                                                                    gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                <  1:450334/150‑1 (MQ=255)
                                                                                                                                    gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                >  2:1193652/1‑150 (MQ=255)
                                                                                                                                    gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                >  2:716711/1‑150 (MQ=255)
                                                                                                                                      aaGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTg              <  2:647159/150‑1 (MQ=255)
                                                                                                                                        gCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCt            <  2:1098380/150‑1 (MQ=255)
                                                                                                                                          atcaCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGCACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTg          >  1:589384/4‑150 (MQ=255)
                                                                                                                                           tcaCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGc         >  2:636401/3‑150 (MQ=255)
                                                                                                                                            caCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCg        >  2:1586146/2‑150 (MQ=255)
                                                                                                                                             aCGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCgg       <  1:728160/150‑1 (MQ=255)
                                                                                                                                              cGCCCCATCGCGGAGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTTGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGt      <  1:689363/150‑1 (MQ=255)
                                                                                                                                              cGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTCTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGt      >  1:1180647/1‑150 (MQ=255)
                                                                                                                                                  ccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCATCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGaa  >  1:571950/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAA  >  NC_000913/3731988‑3732283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: