Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,732,146 G→T 5.2% intergenic (+23/‑55) xylF → / → xylG D‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,732,1460GT 5.2% 391.1 / 5.0 140intergenic (+23/‑55)xylF/xylGD‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base G (65/66);  new base T (4/5);  total (69/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 147 total aligned reads displayed.

AGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAAT  >  NC_000913/3731997‑3732295
                                                                                                                                                     |                                                                                                                                                     
aGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTATGCGTTACGCCCCAGCGCGGAGCg                                                                                                                                                       >  1:1098380/1‑150 (MQ=255)
  aTACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGAGACCGATCGATGTGAATATAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCggg                                                                                                                                                     <  1:199986/150‑1 (MQ=255)
  aTACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCggg                                                                                                                                                     >  2:450334/1‑150 (MQ=255)
   tACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTTAGCGTTACGCCCCAGCGCGGAGCgggg                                                                                                                                                    >  1:624972/1‑150 (MQ=255)
   tACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGATGTAAGCGTTACGCCCCAGCGCGGAGCgggg                                                                                                                                                    >  2:1429711/1‑150 (MQ=255)
      cacaCTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCg                                                                                                                                                 >  1:431520/1‑150 (MQ=255)
      cacaCTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCg                                                                                                                                                 <  2:577045/150‑1 (MQ=255)
      aacacTTAATAATGGCCTCAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATTTTAATAAAAACAACATCAAAGATACGGTAATTAAAGACTGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCg                                                                                                                                                 <  2:231334/149‑1 (MQ=255)
        cacTGAATAATGGCCTGAAAGATGGCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACACAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTg                                                                                                                                               <  2:1399468/150‑1 (MQ=255)
         acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTCACGCCCCCGCTCCGCGCTGGGGCGtaa                                                                                                                                              <  2:370253/150‑3 (MQ=255)
          cTGAATAATGGCCTGAACGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTCAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaac                                                                                                                                             <  1:489396/150‑4 (MQ=255)
          cTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaac                                                                                                                                             <  2:1362670/150‑4 (MQ=255)
          atGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAt                                                                                                                                             <  1:622376/149‑1 (MQ=255)
            caataatGGCCTGACAGATGTCCCCACCCGCCTCCTGACACCGCTCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAACGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAttt                                                                                                                                           <  2:518342/149‑1 (MQ=255)
               taatGGCCTGAAAGATGTCCCCTCCCGCCTCATGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctc                                                                                                                                        >  1:440775/1‑150 (MQ=255)
                aatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGACTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctct                                                                                                                                       <  1:701234/150‑1 (MQ=255)
                  tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTcc                                                                                                                                     <  2:878219/150‑1 (MQ=255)
                  tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTcc                                                                                                                                     <  2:1399985/150‑1 (MQ=255)
                   ggCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGCTTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCa                                                                                                                                    >  1:1058659/1‑150 (MQ=255)
                   ggCCTGAAAGATGTCCCCTCCCCCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGCGCGAGCTGTAATCGTAACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCa                                                                                                                                    >  2:1651528/1‑150 (MQ=255)
                    gCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCAt                                                                                                                                   >  2:740555/1‑150 (MQ=255)
                     ccTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATg                                                                                                                                  >  1:674040/1‑150 (MQ=255)
                     ccTGAAAGATGTCCCCTCCCGCATCCTGACACCGATCGATGTGAATAAAAAAAACATCAAAGATACGGTAATTAAAGACGCATTCCACAAAGAGAGCGAGCTTTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGGGATTTCTCTCCCTg                                                                                                                                  >  2:1636996/1‑150 (MQ=255)
                      cTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGc                                                                                                                                 <  2:709460/150‑1 (MQ=255)
                       tGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGcc                                                                                                                                >  2:1703749/1‑150 (MQ=255)
                        taaaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCCCAAAGAGCGCGAGCTGTAAGCGTTCCGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcg                                                                                                                               <  2:214679/149‑1 (MQ=255)
                        gAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGCTTCCACAAAGAGAGCGAGCTCTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGcag                                                                                                                               >  1:279683/1‑148 (MQ=255)
                        gAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcg                                                                                                                               <  2:1280852/150‑1 (MQ=255)
                         aaaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgc                                                                                                                              <  2:1028099/150‑1 (MQ=255)
                            gATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtg                                                                                                                           >  2:1688935/1‑150 (MQ=255)
                              tGTCCCCTCCCGCCTCCTGACACCGATCGATTTGAATAAAAAGAACATCAACGATACGGTAATTAAAGCCGGATTCCAGAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaa                                                                                                                         <  1:1575173/150‑1 (MQ=255)
                               gTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaat                                                                                                                        <  2:1320309/150‑1 (MQ=255)
                                 ccccTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatga                                                                                                                      >  2:1006906/1‑150 (MQ=255)
                                       ccGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGc                                                                                                                >  2:1153219/1‑150 (MQ=255)
                                        cGCCCCCTGATACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCACGTGAATGAATTGGCt                                                                                                               <  2:45631/150‑1 (MQ=255)
                                         tcctgctGACACCGATCGATTTGAATAAAAACAACATCAATGATACGGTAATTAAAGACGGATTCCACATAGAGTGGGAGCGGTATGCGTTACGCCCCAGCGCGGAGCGGGTGCGTGATTTCTCTCCATGCGGCGTGAATGATTTGGCtt                                                                                                              <  1:69651/149‑1 (MQ=255)
                                           ctcctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCAATACAGCGTGAATGAATTGGCTTAg                                                                                                            >  1:634651/1‑150 (MQ=255)
                                               tGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTgg                                                                                                        <  1:1724838/150‑1 (MQ=255)
                                                   acCGATCGATGTGAATAAAAACAACATAAAAGATACGGTAATTAACGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGTGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTc                                                                                                    <  2:1458556/150‑1 (MQ=255)
                                                    ccGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCg                                                                                                   >  2:365346/1‑150 (MQ=255)
                                                      gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                                 <  2:1384609/150‑1 (MQ=255)
                                                      gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                                 >  1:545253/1‑150 (MQ=255)
                                                          gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGc                                                                                             >  1:381208/1‑150 (MQ=255)
                                                          gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCGTAGGTGGAGTCGTTATGc                                                                                             >  1:1564242/1‑150 (MQ=255)
                                                           atGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGcc                                                                                            <  1:379303/150‑1 (MQ=255)
                                                           atGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGGAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGcc                                                                                            <  1:1114521/150‑1 (MQ=255)
                                                            tgtgAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCt                                                                                           >  1:1654271/1‑150 (MQ=255)
                                                             gtgAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCtt                                                                                          >  2:292054/1‑150 (MQ=255)
                                                               gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGTCGCGTGAATGAATTGGGTTAGGTGGAGTCGTTATGCCtttt                                                                                        >  2:482793/1‑148 (MQ=255)
                                                               gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTAt                                                                                        >  1:476753/1‑150 (MQ=255)
                                                                aaTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATc                                                                                       >  1:797442/1‑150 (MQ=255)
                                                                 aTAAAAACAACATCAAAGATACGGTATTTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                                      >  1:1473279/1‑150 (MQ=255)
                                                                 aTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                                      <  2:1534992/150‑1 (MQ=255)
                                                                 aTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                                      >  2:1246329/1‑150 (MQ=255)
                                                                   aaaaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTAc                                                                                    >  2:1011648/1‑150 (MQ=255)
                                                                    aaaaCATCATCAAAGTTACGGTAATTAAAGTCGGTTTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                                   <  2:411883/150‑1 (MQ=255)
                                                                    aaaaCAACCTCAAAGATCGGGTAATTACAGACGGATTACACCAAGAGAGCGAGAGGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                                   <  2:137601/150‑1 (MQ=255)
                                                                      aacaacATCAAAGATACGGTAATTACAGACGGCTTCCCCAAAGAGAGCGTGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTg                                                                                 <  2:1333342/150‑1 (MQ=255)
                                                                            aTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTAATTTAAATga                                                                           >  2:1422406/1‑150 (MQ=255)
                                                                             tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaa                                                                          >  1:1389967/1‑150 (MQ=255)
                                                                              cAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaag                                                                         >  1:248686/1‑150 (MQ=255)
                                                                               aaaGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaaga                                                                        <  1:1703749/150‑1 (MQ=255)
                                                                                 aGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAAc                                                                      <  1:1559207/150‑1 (MQ=255)
                                                                                  gATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACa                                                                     <  1:1135158/150‑1 (MQ=255)
                                                                                  gATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACa                                                                     <  2:475444/150‑1 (MQ=255)
                                                                                        gTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCa                                                               >  1:1021658/1‑150 (MQ=255)
                                                                                        gTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCa                                                               <  1:1490161/150‑1 (MQ=255)
                                                                                        gTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCACCAGCGCGGAGCTGGTGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCa                                                               >  1:278367/1‑150 (MQ=255)
                                                                                          aaTTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGATTTGGCTTAGGTGGAGTCGTTATGCCTGATCTACTTGAAATGAAGACCATTACCaaa                                                             >  2:1068289/1‑150 (MQ=255)
                                                                                           aTTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCaaaa                                                            >  1:350026/1‑150 (MQ=255)
                                                                                            ttAATGACGGAGTCTACAACGACAGCGCGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGTCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGGCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAAc                                                           <  2:281192/150‑1 (MQ=255)
                                                                                               aaGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCtt                                                        >  2:1351892/1‑150 (MQ=255)
                                                                                                 gACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGTAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCg                                                      <  1:1051430/150‑1 (MQ=255)
                                                                                                     gATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTCGCTTAGGTGGAGTCGTTATGCCTTATCTCCTTGAAATGAAGCACATTACCAACACCTTCGGCAg                                                  >  1:55267/1‑150 (MQ=255)
                                                                                                      aTTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAgt                                                 >  1:1053437/1‑150 (MQ=255)
                                                                                                         ccACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGtgtg                                              >  1:1450970/1‑150 (MQ=255)
                                                                                                             aaaGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAgg                                          <  1:1611458/150‑1 (MQ=255)
                                                                                                                   agcgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTg                                    >  2:507401/1‑150 (MQ=255)
                                                                                                                       agcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATaa                                >  1:1049719/1‑150 (MQ=255)
                                                                                                                        gcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTGCATGCCGCGTGAATGAATTGGCGGAGGTGGAGTCGGTATGGCTTATCTACTTGCATTGACGTACATTACCAAAACCTGGGGGCGTGGGATGGGGTTTGATagc                               >  1:330422/1‑148 (MQ=255)
                                                                                                                        gcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAAc                               >  2:1330502/1‑150 (MQ=255)
                                                                                                                        gcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAAc                               <  1:740555/150‑1 (MQ=255)
                                                                                                                         cTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACg                              >  2:387852/1‑150 (MQ=255)
                                                                                                                           gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                            >  2:1193652/1‑150 (MQ=255)
                                                                                                                           gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                            >  2:716711/1‑150 (MQ=255)
                                                                                                                           gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                            <  2:431520/150‑1 (MQ=255)
                                                                                                                            tAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCt                           <  2:797442/150‑1 (MQ=255)
                                                                                                                               gCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCTTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCt                        <  2:737888/150‑1 (MQ=255)
                                                                                                                                  tcaCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGc                     >  2:636401/3‑150 (MQ=255)
                                                                                                                                   caCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCg                    >  2:1586146/2‑150 (MQ=255)
                                                                                                                                    aCGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCgg                   <  1:728160/150‑1 (MQ=255)
                                                                                                                                     cGCCCCATCGCGGAGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTTGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGt                  <  1:689363/150‑1 (MQ=255)
                                                                                                                                     cGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTCTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGt                  >  1:1180647/1‑150 (MQ=255)
                                                                                                                                         ccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCATCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGaa              >  1:571950/1‑150 (MQ=255)
                                                                                                                                             cgcgGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCt          <  1:566852/150‑1 (MQ=255)
                                                                                                                                               cgGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTgg        <  1:1246329/150‑1 (MQ=255)
                                                                                                                                               cgGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTgg        <  1:473230/150‑1 (MQ=255)
                                                                                                                                               cgGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGGCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTgg        <  2:1058659/150‑1 (MQ=255)
                                                                                                                                                  aGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTTCCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGa     <  2:1127232/150‑1 (MQ=255)
                                                                                                                                                  aGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGGGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGa     <  1:1193652/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAAT  >  NC_000913/3731997‑3732295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: