Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,757,945 T→G 6.9% intergenic (‑118/+72) yiaY ← / ← selB L‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,757,9450TG 6.9% 538.4 / 21.4 194intergenic (‑118/+72)yiaY/selBL‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor
Reads supporting (aligned to +/- strand):  ref base T (111/67);  new base G (9/4);  total (123/71)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-01

Only 100 of 197 total aligned reads displayed.

CACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTCA  >  NC_000913/3757795‑3758092
                                                                                                                                                      |                                                                                                                                                     
 acaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGTCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAtt                                                                                                                                                       <  1:49561/150‑1 (MQ=255)
  aataaGGAATTAAGAACGTCGAAGCTGCCATAACACACTCCTGTAAGTGTAAAAAAGGGCGGGCAGCTTACTGATGATTTTCATTCGAAAATATGATCAGTATTTTTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTa                                                                                                                                                      <  2:1339122/147‑1 (MQ=255)
   aGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTACTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTaa                                                                                                                                                     <  1:794102/150‑1 (MQ=255)
       ggAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACaa                                                                                                                                                >  1:1036288/1‑150 (MQ=255)
             aaGAACGTTGAAGCTTCCATAACACTATACTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGCt                                                                                                                                          <  2:88687/150‑1 (MQ=255)
             aaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCt                                                                                                                                          >  2:405176/1‑150 (MQ=255)
             aaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCt                                                                                                                                          <  1:1566992/150‑1 (MQ=255)
               gAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttt                                                                                                                                        >  1:981864/1‑150 (MQ=255)
               gAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGAACATTGTATTCATAAAACAATGTGCttt                                                                                                                                        >  2:163459/1‑150 (MQ=255)
                 aCGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttttt                                                                                                                                      >  1:481877/1‑150 (MQ=255)
                 aCGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttttt                                                                                                                                      >  2:352175/1‑150 (MQ=255)
                    ttGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTa                                                                                                                                  <  2:1287239/150‑1 (MQ=255)
                    gtGAATCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAACAGCACATTGTATTCATTAAACAATGTGCTTTTTTTa                                                                                                                                  <  1:713005/149‑1 (MQ=255)
                     tgtaGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTaa                                                                                                                                 <  2:192869/147‑1 (MQ=255)
                     tGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTaa                                                                                                                                 >  2:951576/1‑150 (MQ=255)
                      gAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTaaa                                                                                                                                >  2:32657/1‑150 (MQ=255)
                       aaGCTGCCATAACACACTCCTGAAATTGTAAAAAAGTGCGGGCAGCTTACTGAGGATTTTCATTAGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTtaaa                                                                                                                               <  1:217422/150‑2 (MQ=255)
                           tGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGTCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCAt                                                                                                                           <  1:128572/150‑1 (MQ=255)
                            gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                          <  1:1422641/150‑1 (MQ=255)
                             ccATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATaa                                                                                                                         >  2:1172744/1‑150 (MQ=255)
                             ccATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATaa                                                                                                                         >  2:702263/1‑150 (MQ=255)
                             ccATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATaa                                                                                                                         <  2:927541/150‑1 (MQ=255)
                                tAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGaa                                                                                                                      >  2:372674/1‑150 (MQ=255)
                                   cacacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAAttt                                                                                                                   >  2:1415666/1‑150 (MQ=255)
                                   cacacTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAAttt                                                                                                                   >  1:1067957/1‑150 (MQ=255)
                                      acTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGt                                                                                                                <  1:362614/150‑1 (MQ=255)
                                      acTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGt                                                                                                                <  2:223476/150‑1 (MQ=255)
                                        tCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTAt                                                                                                              >  1:878560/1‑150 (MQ=255)
                                         ccTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTAtt                                                                                                             >  1:705041/1‑150 (MQ=255)
                                         acTGAAATTGTAAAAAATGGCGGGCAGCTTACTGAGGATTTTCATTCGATAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTAtt                                                                                                             <  1:1233645/149‑1 (MQ=255)
                                               aGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAattta                                                                                                       >  1:562307/1‑150 (MQ=255)
                                                   tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAAATTAtttt                                                                                                   >  1:299187/1‑150 (MQ=255)
                                                    aaaaaaGGGCGGGCAGCATACTGAGGATTTTCATTCGAAAAAATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCAAATTGTATTCATTAAACAATTTTCTTTTTTTAAATTCATAAGAAATATGTATTAAATTATTTTa                                                                                                  >  1:258454/1‑150 (MQ=255)
                                                              tggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTTATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatt                                                                                        <  2:269384/149‑1 (MQ=255)
                                                              gggcAGCTTACTGATGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatt                                                                                        <  1:991789/150‑1 (MQ=255)
                                                               ggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatta                                                                                       >  2:910881/1‑150 (MQ=255)
                                                                        cTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTca                                                                              >  1:90988/1‑149 (MQ=255)
                                                                             gATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCattatt                                                                         >  1:323145/1‑150 (MQ=255)
                                                                                 ttCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTc                                                                     <  2:263399/150‑1 (MQ=255)
                                                                                  tCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTcc                                                                    <  2:1036288/150‑1 (MQ=255)
                                                                                  tCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTcc                                                                    <  1:1639851/150‑1 (MQ=255)
                                                                                   cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                   <  1:1391571/150‑1 (MQ=255)
                                                                                   cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                   <  1:955737/150‑1 (MQ=255)
                                                                                    aTTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTcctg                                                                  >  2:536936/1‑148 (MQ=255)
                                                                                    aTTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCgg                                                                  <  2:524237/150‑1 (MQ=255)
                                                                                     ttCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGa                                                                 <  2:560080/150‑1 (MQ=255)
                                                                                     ttCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGa                                                                 >  2:1535571/1‑150 (MQ=255)
                                                                                     ttCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGa                                                                 <  1:1120515/150‑1 (MQ=255)
                                                                                           aaTATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTAATTAAACAATGTGATTTTTTTAAATTAATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCTGAaataaa                                                           >  1:853354/1‑149 (MQ=255)
                                                                                               tGATCAGTATTATTGATTATCAAAATTAATCTAATAATAAGCACATTGTATTCATTTAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaatgatcatt                                                       <  1:1538043/150‑2 (MQ=255)
                                                                                                     gTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTcgc                                                 >  2:589180/1‑150 (MQ=255)
                                                                                                       attattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATTAGAATTTTGTATTAATTTATTTTAAAACTTATTTATTCATTTCCTTATTTTTCCGGAATTTATAATGCGTCgcgc                                               >  2:306717/1‑150 (MQ=255)
                                                                                                       attattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGATTTTTTTAAATTCATAAGAATTTTGTATTAATTTATAATAAAACTTAATTAATCATATCCTTATTTTTCCTGAAATAATAATGCGTcgctc                                               >  1:525296/1‑148 (MQ=255)
                                                                                                        ttattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCa                                              >  1:446016/1‑150 (MQ=255)
                                                                                                          attGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGATTTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAAAAATGCGTCGCGCAAt                                            >  2:123517/1‑150 (MQ=255)
                                                                                                              aTTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAAt                                        >  1:1457550/1‑150 (MQ=255)
                                                                                                               ttATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATTCTTCGCGCAATAAATg                                       >  2:289059/1‑150 (MQ=255)
                                                                                                                tATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCTCAATAAATGa                                      >  1:1306781/1‑150 (MQ=255)
                                                                                                                tATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGa                                      >  2:73725/1‑150 (MQ=255)
                                                                                                                 aTCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGAGCAATAAATGAt                                     >  2:125554/1‑150 (MQ=255)
                                                                                                                  tCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATc                                    <  1:679821/150‑1 (MQ=255)
                                                                                                                  tCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATATTAAAACTTAATTAATCATTTCCTTATTTTACCGGAAATAATAATGCGTATCGCAATAAATGATc                                    >  2:209526/1‑150 (MQ=255)
                                                                                                                        ttAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTcc                              <  1:621154/150‑1 (MQ=255)
                                                                                                                        ttAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTcc                              <  2:611294/150‑1 (MQ=255)
                                                                                                                         tAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCa                             >  2:1548526/1‑150 (MQ=255)
                                                                                                                          aaTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCac                            >  2:49337/1‑150 (MQ=255)
                                                                                                                          aaTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCac                            <  2:1483882/150‑1 (MQ=255)
                                                                                                                           aTCTAATAAAAATCACATTGTATTAATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                           <  1:1258274/150‑1 (MQ=255)
                                                                                                                           aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                           <  2:228981/150‑1 (MQ=255)
                                                                                                                             cTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgac                         >  2:1713322/1‑150 (MQ=255)
                                                                                                                              taataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacg                        >  2:871954/1‑150 (MQ=255)
                                                                                                                              taataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAAATTAATTAATCATTTCCTTATTTTTCCGTAAATAATAATGCGTCGCGCAATAAATGATCATTTCCaagacg                        >  2:891458/1‑150 (MQ=255)
                                                                                                                               taaaaaaaGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                                   <  2:1295377/103‑1 (MQ=25)
                                                                                                                               aataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacga                       >  2:709060/1‑150 (MQ=255)
                                                                                                                               aataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacga                       >  1:1684890/1‑150 (MQ=255)
                                                                                                                                ataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTACGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacgac                      >  2:1205094/1‑150 (MQ=255)
                                                                                                                                   aaaaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGAcgcg                   <  2:981864/150‑1 (MQ=255)
                                                                                                                                   aaaaGCACATTGTTTAATGAATACAATGTGCTTTTTATTAGATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGAcgccgc                    >  2:849992/1‑150 (MQ=37)
                                                                                                                                     aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa                 <  1:530333/150‑1 (MQ=255)
                                                                                                                                      aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAAt                                        >  2:1110936/1‑126 (MQ=255)
                                                                                                                                      aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa                >  2:318929/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa                >  2:594753/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa                >  2:105437/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa                >  1:1215271/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa                >  2:409567/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa                >  1:794034/1‑150 (MQ=255)
                                                                                                                                      aGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa                >  2:748527/1‑150 (MQ=255)
                                                                                                                                       gCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaaa               >  1:810330/1‑150 (MQ=255)
                                                                                                                                       gCACATTGTATTCATTAAACAATGTGCTTTTTTTAAAATCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaaa               >  2:1662436/1‑150 (MQ=255)
                                                                                                                                        cacaTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAg              >  1:1278850/1‑150 (MQ=255)
                                                                                                                                         acaTTTTATTCATTAAAAAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTAATTTTAAAACTTAATTAATCATTTGGTTATTTTTGCGGAAATAATAATGCGTCGCGCAATATATGATCATTTCCACGACGACGCGTAAAGc             <  2:1146399/150‑1 (MQ=255)
                                                                                                                                         acaTTCTATTAATTAAAAAATTTGCTTTTTTTAAATTAATAAGAATTTTGTATTTATTTATTTTTAAACTTAATTAATCATTTCCTTATTTTTCAGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAga             <  1:209526/150‑2 (MQ=255)
                                                                                                                                            ttGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCaa          >  2:1595187/1‑150 (MQ=255)
                                                                                                                                               tATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGc       >  2:169489/1‑150 (MQ=255)
                                                                                                                                                 ttCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCgg     <  1:1150210/150‑1 (MQ=255)
                                                                                                                                                  tCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGt    >  2:1224255/1‑150 (MQ=255)
                                                                                                                                                   cATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTc   >  2:415606/1‑150 (MQ=255)
                                                                                                                                                   aaTTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTc   <  2:723548/149‑1 (MQ=255)
                                                                                                                                                    aTTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTCa  >  2:821587/1‑150 (MQ=255)
                                                                                                                                                    aTTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTCa  >  2:1135443/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATGCGGTCA  >  NC_000913/3757795‑3758092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: